• KSBB Journal
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• v.18 no.6
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• pp.435-439
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• 2003

Plant cell culture can be divide into two classes non-organic culture and organic culture. Non-organic culture such as suspension culture has many researches, however organic culture about recombinant protein production has little researches. Recombinant protein produced through organ culture is quite stable and it can make proteins by itself without any grow regulators. Therefore organ culture is much easier than other methods. In this research, we used transformed tobacco seed. At first we germinated the seed then separated stems and leaves from the grown plant. And raised in liquid medium by in vitro vegetative reproduction. Continuing most suitable conditions, we compared the Quantities of recombinant protein from intra cellular with from extra cellular. And adding some permeabilizing agents (Pluronic F-68, Triton X-100, DMSO, PEG8000), we increased the productivity of the recombinant protein.

• Korean Journal of Plant Tissue Culture
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• v.26 no.4
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• pp.223-227
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• 1999

This study was carried out to establish improved techniques on organogenesis from callus culture of Gladiolus. Organogenesis from the callus was effective in the half strength of MS solid medium without 2,4-D at 15 $^{\circ}C$ under 24 hours of daylength. Formation of adventitious root was most effective in the liquid shaking culture, and adventitious shoot induction was effective in the liquid stationary culture. From these results, we could find optimal culture conditions for redifferentiation from callus, in addition, liquid shaking culture revealed as more useful when compared with that of solid culture method for the redifferentiation of callus in Gladiolus `Topaz'.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2023.04a
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• pp.35-35
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• 2023

안정적인 유묘의 확보는 스마트작물생산을 위한 공정육묘 생산에서도 중요하며, 기내배양시 유전적 안정성이 높은 유묘의 대량증식은 유묘생산과 공정육묘생산에서 중요한 과정이다. 기내배양시 배양과 정에서 존재하는 체세포영양계변이(somaclonal variation)라는 장벽을 제거하는 것이 중요하다. 본 연구에서는 화본과 식물인 홍띠(Imperata cylindrica ‘Rubra’)로부터 기관분화 단계별 재분화체를 작성하여 기관분화 시 기내재생체의 유전적 안정성을 조사하였다. ISSR 마커에 기반하여 유전적 변이성을 조사하고자 7종류 총 21개체의 기관분화 단계별 재분화체 및 재분화식물체에 대하여 분석한 결과, 유전적 다형성은 기관분화 단계별 재분화체 및 순화 재분화체에서 대조구인 모식물체(1.4％) 대비 같거나 높게 나타나서 재분화체에서 유전적 안정성이 다소 낮은 것으로 나타났다. 또한, Jaccard 계수(Jaccard coefficient)로 총 21개체들 간의 유전적 유사도 지수를 평가한 결과, 유전적 유사도 지수는 0.747~1.0 사이에 분포하며, 평균 0.868로 나타났다. ISSR 마커 밴드에 기반하여 평균연결법(Average linkage method)으로 군집 분석한 결과, 모든 개체는 유사도 지수 0.809 ~ 1.000 내에 분포하였다. 유전적 유사도 지수 0.809에서 2개 그룹으로 유집되었으며, 모식물체와 실내재배, 노지재배 재분화 녹색 식물체가 같은 그룹으로 분류되었다. 이상의 결과는 화본과 식물의 기내배양에서 기관분화 시 존재하는 체세포영양계변이에 대한 기초 정보를 제공해 준다. 이들 기관분화에 따른 기내재생체의 안정성에 대한 연구자료는 향후 기내식물의 안정적인 대량번식에 있어 유익한 배경을 제공해 줄 것이다.

• Proceedings of the KAIS Fall Conference
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• 2006.11a
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• pp.287-289
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• 2006

곤충 혈림프에서 존재하는 리포포린은 선택적으로 지질을 지질 사용및 저장기관으로 운반한다. 본 연구는 유충지방체, 성충난소 및 정소로 지질의 운반과 유충지방체 및 성충난소로 리포포린 자체가 흡수되는 과정을 조사하였다. 이들의 기능을 조사하기 위해 FITC-labeled 리포포린과 DiI-labeled 리포포린을 사용하였다. 유충지방체, 성충난소 및 정소를 DiI-labeled 리포포린과 배양한 결과 리포포린으로 부터 각 기관으로 지질을 운반함을 알 수 있었고, 또한 receptor-mediated endocytosis 억제제인 suramin, unlabeled 리포포린과 배양한 결과는 리포포린에서 각 기관으로 운반되는 지질의 양이 현저하게 감소함을 알 수 있었다. 또한, 유충지방체와 성충난소를 FITC-labeled 리포포린과 배양한 결과 위에서 언급한 지질 뿐만 아니라 리포포린 자체도 각 기관의 에너지원으로 사용하기 위해 흡수된다는 사실을 알 수 있었으며, suramin과 unlabeled 리포포린과 배양한 결과 리포포린 자체가 흡수되는 양이 현저하게 감소함을 알 수 있었다. 위 실험결과로부터 리포포린에 의한 지질의 운반과정과 리포포린 자체의 흡수과정이 receptor-mediated endocytosis로 이루어짐을 알 수 있었다.

• Parasites, Hosts and Diseases
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• v.25 no.1
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• pp.24-36
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• 1987

The effect of praziquantel on P. westermani exposed in vitro was observed by stereomicroscope, light microscope and scanning electron microscope. Following results were found. 1. The worms incubated in $0.01{\;}{\mu}g/ml$ praziquantel were moving after 26-hour incubation. However, all of them were immobilized immediately after incubation in solutions over $0.01{\;}{\mu}g/ml$ concentration. 2. All of the exposed worms showed severe vacuolization not only in tegument but in subtegument, intestine, ovary, testis, Mehlis' gland and excretory bladder. 3. Vacuoles in tegument burst out to form craters. As incubation time went on, tegumental structure was disintegrated severely. The worms exposed to praziquantel were observed to be immobilized and be vacuolized of all tissues. Disintegration of reproductive organs suggests that praziquantel have suppressive effect on egg production when the flukes are not killed. The drug effects were found more related with incubation time than with drug concentration.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.29 no.4
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• pp.401-408
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• 1984

This study was conducted to determine the optimum concentrations of growth regulators and their responses on the clonal propagation in axillary bud culture. Cultivars, Hongmi and Shinmi, responded differently to the levels of growth regulators, proliferation rate and shoot growth. The shoot and root of Hongmi cultivar in axillary bud culture were conspicuously induced by combination of NAA(0.1mg/l) and Kinetin(1mg/l) while Shinmi cultivar were affected by the single concentration of Kinetin(1mg/l) and BA(0.1mg/l), and also by the combination of NAA(0.1mg/l) and Kinetin(1mg/l). Better shoot growth and root initiation were obtained in the combination of NAA(0.1mg/l) and Kinetin(1mg/l) regardless of cultivars used when 5mm axillary buds were cultured. The shoots regenerated at the high levels of BA(1-5mg/l) were abnormally thicker and narrower leaves than normal plants and short in shoot height. Frequencies of abnormal plants were higher than that of the low level (0.1mg/l) of BA.

• Korean Chemical Engineering Research
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• v.44 no.1
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• pp.58-64
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• 2006

We successfully isolated human anagen hair follicles from human scalp skin by microdissection and tried to culture them under various conditions. First we confirmed negative effect of serum on human hair follicle organ culture. As a next step serum-free medium compositions, Philpott medium, IMDM, and DHGM (Dongguk hair growth medium) were tried. Philpott medium is a general medium for hair organ culture based on Williams' E medium and DHGM is a special self-developed medium containing high amino acids and vitamins (B group) composition. As results, hair follicle in Philpott medium and IMDM showed anagen phase morphological structure, but rapid loss of hair elongation, low alkaline phosphatase expression, and very low expression of CK19. It is thought these hair follicles rapidly regressed from apoptosis. However, hair follicles in DHGM showed long term anagen phase morphological structure, continuous hair elongation, high alkaline phosphatase, and CK19 expression. These results demonstrate that high amino acids and vitamins (B group) composition are essential to in vitro long term human hair follicle organ culture and this culture medium will be useful in basic study of hair biology or application study to the development of alopecia treatment drugs.

• Neonatal Medicine
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• v.18 no.2
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• pp.240-247
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• 2011

Purpose: The rationale for skin surface cultures is that bacterial colonization precedes infection and, as a result, that identification of a potential pathogen is predictive of later systemic infection in preterm infants. We aimed to analyze results of surveillance surface cultures in extremely low birth weight (ELBW) infants and seek for effective ways of performing surveillance surface cultures. Methods: We analyzed the surveillance surface cultures of 113 ELBW infants over a 4-year period. Surveillance cultures were obtained routinely from five sites: axilla, external ear canal, nasopharynx, throat (or tracheal aspirate if intubated) and anus. Each surface culture obtained during the 13 days, prior to the date of the blood culture, was compared with the blood culture obtained when sepsis was suspected. The sensitivity, specificity and positive predictive value (PPV) of the surveillance cultures were calculated among 1894 blood-surface culture pairs by surface sites, recovered organisms and interval between surveillance samples and blood cultures. Results: The overall sensitivity, specificity and PPV of surface cultures were 45.9, 22.4 and 6.8%, respectively. The PPV was highest for the throat/tracheal cultures (11.0%) and lowest for the anal cultures (2.3%). As the time of culturing progressed toward the day of blood culturing, the sensitivity and specificity of the surface cultures significantly increased. Only axillary and throat/tracheal cultures were useful in predicting the microorganisms causing sepsis. Conclusion: Surface cultures could help to predict sepsis pathogens and infection surveillance in preterm infants could be continued with a reduced number of cultured sites focusing on the axilla and throat/trachea.

• Journal of Embryo Transfer
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• v.17 no.3
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• pp.195-201
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• 2002

This study was to assess the developmental capacity of oocytes matured in vitro after 20, 15, 10, 5 and 0 days of organ culture when ovaries were isolated from juvenile mice at 0-, 5-, 10-,15- and 20-day old, respectively, and to develop in vitro culture system that observed a view to morphology of ovaries and nucleus maturation of oocytes. The size of ovaries decreased 35.9%, 8.7%, 1.2% and 14.4% after 20, 15, 10, 5 days of organ culture when the ovaries were isolated from 0-, 5-, 10 and 15-day old mice, respectively. After organ culture, the recovery rates, diameters of oocytes and the number of oocytes progressed from GV to MII were increased as increasing age of mice.

• Journal of Life Science
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• v.14 no.3
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• pp.375-384
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• 2004

Fibroblast growth factors (FGFs) are known to induce multiple functions in early development, including mesoderm formation, gastrulation movement and antero-posterior patterning. The induction of mesoderm from Xenopus presumptive ectoderm and the combination effect on inducing organs of bFGF(basic FGF) and HGF (Hepatocyte Growth Factor) were studied. Explants were cultured in the combined solution for 3 days to normal embryo arrive at St. 43. These effects on combined dose were examined by histological experiment and by immunohistochemical method. The concentrations of growth factors were tested in 0, 0.5, 1, 10 and also tested in 50 ng/ml of bFGF, and 0, 1, 10, 50 and 100ng/ml of HGF respectively. The synergistic effects were seen in the combined-dose of bFGF and HGF rather than in single dose. Various organs were differentiated and highest inducing effects were seen at the combined concentration of 1 ng/ml of bFGF and 10ng/ml of HGF, and at the concentration 10ng/ml of bFGF and 1 ng/ml of HGF. The bFGF induces various organs from cultured animal cap explants and the effects are time and dose-dependent. HGF is also a potent mitogen for renal tubular cells and for mature hepatocytes in primary culture. Eyes were developed in high percentage at the combined concentration of 1 and 10ng/ml of bFGF, and 1 and 10 ng/ml of HGF. From the induced eye and normal embryonic eye, RPE65 was commonly detected by monoclonal antibodies 40All and 25F5 and the localization of RPE65 was seen by AP reaction.