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Organ Culture of Ovary Isolated from Juvenile Mice  

이현주 (대구대학교 자연자원대학)
김지철 (대구대학교 자연자원대학)
김기동 (고려대학교 생명공학원)
이상호 (고려대학교 생명공학원)
송해범 (대구대학교 자연자원대학)
Publication Information
Journal of Embryo Transfer / v.17, no.3, 2002 , pp. 195-201 More about this Journal
Abstract
This study was to assess the developmental capacity of oocytes matured in vitro after 20, 15, 10, 5 and 0 days of organ culture when ovaries were isolated from juvenile mice at 0-, 5-, 10-,15- and 20-day old, respectively, and to develop in vitro culture system that observed a view to morphology of ovaries and nucleus maturation of oocytes. The size of ovaries decreased 35.9%, 8.7%, 1.2% and 14.4% after 20, 15, 10, 5 days of organ culture when the ovaries were isolated from 0-, 5-, 10 and 15-day old mice, respectively. After organ culture, the recovery rates, diameters of oocytes and the number of oocytes progressed from GV to MII were increased as increasing age of mice.
Keywords
organ culture; juvenile mice; ovary; oocyte;
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