Journal of Embryo Transfer (한국수정란이식학회지)

The Korean Society of Embryo Transfer (한국수정란이식학회)
권호 표지

Organ Culture of Ovary Isolated from Juvenile Mice

약령 마우스에서 분리한 난소의 기관배양에 관한 연구

  • 이현주 (대구대학교 자연자원대학) ;
  • 김지철 (대구대학교 자연자원대학) ;
  • 김기동 (고려대학교 생명공학원) ;
  • 이상호 (고려대학교 생명공학원) ;
  • 송해범 (대구대학교 자연자원대학)
  • Published : 2002.12.01
Download PDF
⟨ Previous Next ⟩

Abstract

This study was to assess the developmental capacity of oocytes matured in vitro after 20, 15, 10, 5 and 0 days of organ culture when ovaries were isolated from juvenile mice at 0-, 5-, 10-,15- and 20-day old, respectively, and to develop in vitro culture system that observed a view to morphology of ovaries and nucleus maturation of oocytes. The size of ovaries decreased 35.9%, 8.7%, 1.2% and 14.4% after 20, 15, 10, 5 days of organ culture when the ovaries were isolated from 0-, 5-, 10 and 15-day old mice, respectively. After organ culture, the recovery rates, diameters of oocytes and the number of oocytes progressed from GV to MII were increased as increasing age of mice.

본 실험은 원시난포의 체외배양 체계를 확립할 수 있는 가능성을 검토하기 위해 0, 5, 10, 15 및 20일령 마우스에서 분리한 난소를 20, 15, 10, 5 및 0일동안 organ culture 하여 난소의 성장률, 난자의 회수율과 성장률 및 난자의 핵성숙 단계를 조사하여, 비교 ·검토하였다. 그 결과를 요약하면 다음과 같다. 1. Organ culture 전과 후의 각 일령에 따른 난소의 면적 차이는 0일령 35.9%, 5일령 8.7%, 10 일령 1.2% 및 15일령 14.4%로 15일령을 제외 하고는 배양일령이 증가할수록 면적 차이는 감소하였다. 2. Organ culture 후 난자의 회수율과 난자의 직경은 배양일령이 증가할수록 증대되었다. 3. GV기 이상의 핵성숙은 organ culture 후 배양 일령이 증가할수록 진행되는 단계에 있는 것으로 나타났다.

Keywords

References

  1. Blandau R, Warrick E and Rumery RE. 1965. In vitro cultivation of fetal mouse ovaries. Fertil. Steril., 16;705-715 https://doi.org/10.1016/S0015-0282(16)35761-2
  2. Caroll J, Whittingham DG and Wood MJ. 1991. Effect of gonadotrophin environment on growth and development of isolated mouse primary ovarian follicles. J. Reprod. Fert., 93:71-79 https://doi.org/10.1530/jrf.0.0930071
  3. Chapeakar TN, Nayak GV and Ranadive KJ. 1996. Studies on the functional activity of organoty-pically cultured mouse ovary. J. Embr. Exp. Morph., 15:133-141
  4. Eppig JJ. 1992. Growth and development of mam-malian oocytes in vitro. Arch Pathol Lab. Med., 116:379-382
  5. Eppig JJ and O'Brien MJ. 1996. Development in vitro of mouse oocytes from primordial folli-cles. Biol. Reprod., 54:197-207 https://doi.org/10.1095/biolreprod54.1.197
  6. Eppig JJ and Schroeder AC. 1989. Capacity of mouse oocytes from preantral follicles to un-dergo embryogenesis and development to live young after growth, maturation and fertilization in vitro. Biol. Reprod., 41:268-276 https://doi.org/10.1095/biolreprod41.2.268
  7. Fainstat T. 1968. Organ culture of postnatal rat ovaries in chemically defined medium. Fertil Steril., 19:317-338
  8. Leibfried L and First NL. 1980. Follicular control of meiosis in the porcine oocyte. Biol. Reprod., 23:705-709 https://doi.org/10.1095/biolreprod23.4.705
  9. Ninomiya T, Hoshi M, Mizuno A, Nagao M and Yuki A. 1989. Selection of mouse preimplanta-tion embryos carrying exogenous DNA by polymerase chain reaction. Mol. Reprod. Dev., 1:242-248 https://doi.org/10.1002/mrd.1080010404
  10. Odor DL and Blandau RJ. 1971. Organ cultures of fetal mouse ovaries. I. Light microscopic struc-ture. Am. J. Anat., 131:387-414 https://doi.org/10.1002/aja.1001310402
  11. Racowsky C and Baldwin K.V. 1989. In vitro and in vivo studies reveal that hamster oocyte meiotic arrest is maintained only traiisiently by follicular fluid, but persistently by membrane/cumulus granulosa cell contact. Dev. Biol., 134:297-306 https://doi.org/10.1016/0012-1606(89)90102-4
  12. Tsafriri A and Channing CP. 1975. An inhibitory influence of granulosa cells and follicular fluid upon porcine oocyte meiosis in vitro. Endorcr-inology, 96:922-927
  13. Tsafriri A, Dekel N and Bar-Ami S. 1982. Role of oocyte maturation inhibitorin follicular regula-tion of oocyte maturation. J. Reprod. Fert., 64:541-551 https://doi.org/10.1530/jrf.0.0640541
  14. Whittingham DG. 1971. Culture of mouse ova. J Reprod. Fert. Suppl., 14:7-12