• Proceedings of the Korea Society of Poultry Science Conference
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• 2004.11a
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• pp.31-32
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• 2004

In order to at day evaluate effect of dietary 2.0 % brown seaweed(Undaria pinnatifida) with bean extract on performance and nutrients utilization during acute phase response, one day-old broiler chicks were fed basal diet and diets substituted with 2.0 % brown seaweed and 2.0 % brown seaweed with bean extract instead of wheat bran in basal diet for 4weeks. Dietary 2.0 % brown seaweed with bean extract enhanced dietary ME value and protein accumulation due to the increased NB and decreased UAN excretion. increased feed intake during acute phase response and had the highest body weight among birds fed experimental diets. The resulted indicated that dietary 2.0 % brown seaweed with bean extract improved performance of broiler chicks due to the increased protein accumulation during acute phase response.

• Proceedings of the Korean Society of Applied Pharmacology
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• 1994.04a
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• pp.238-238
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• 1994

녹용의 알려진 약리작용 중에서 녹용의 상용량 투여에 의해 나타나는 것은 강장작용과 창상치유작용이므로 이러한 작용이 진정한 녹용의 효능으로 간주될 수 있으나 이러한 작용을 생물활성 검색법으로 활용하여 녹용의 유효성분을 검색하기에는 재현성과 간편성이라는 면에서 어려운 점이 없지 않다. 녹용의 효능이 생체의 질소평형이 음의 방향으로 진행되었을 때 이를 정상 수준으로 복귀시키는 생물활성에 기인할 것이라는 가정하에서, 실험적으로 생체내 질소편형을 음의 방향으로 전환시킬 수 있는 급성기 반응 (acute-phase responce)을 야기시킨 다음 녹용의 효과를 연구하였다. 흰쥐의 급성기 반응물질 (acute-phase reactants, APR) 중에 서 positive ARP인 ceruloplasmin, $\alpha$-cysteine pretense inhibitor(= T-kininogen)는 비색정량법으로 간편하게 측정될수 있음에 착안하여 이 단백질을 지표로 삼아 turpentine oil로 급성 염증을 유발시킨 전후에 녹용 물추출액의 효과를 측정하였다. 그 결과 녹용의 상용량에서 괴소 4일 이상 경구투여한 흰쥐에서 급성 염증유발 후 4일에서 ceruloplasmin 의 혈중농도가 현저히 감소함을 알수 있었다. 급성 염증 유발후 녹용의 경구투여한 실험에서는 적어도 녹용 상용량의 3배 이상을 투여하여야 만 ceruloplasmin 혈중 농도를 감소시킬 수 있었다. 본 연구에서 수립된 생물활성을 이용하여 녹용의 유효 성분을 단리할 계획이다.

• Proceedings of the Korea Society of Poultry Science Conference
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• 2003.11a
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• pp.73-74
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• 2003

Three experiment were designed to determine the effects of dietary(n-3) fatty acids on the growth-suppressive effects of the inflammatory response and anti-oxidant enzyme activity and balances of nitrogen and energy. Day-old chicks were fed diets containing 1.0 ％ soybean oil in experiment 1, or diets containing 1.0 ％ of fish oil in experiment 2, and then on 7-d of age chicks were fed diets containing 1.0 ％ of either soybean oil or fish oil. In experiment 3, day-old chicks were fed on diets containing 1.0 ％ of either soybean oil or fish oil. In chicks of half pens within each dietary treatments of each experiments, the acute phase response were induced by injecting with Salmonella typhymurium lipopolysaccaride(LPS) i.p. 3 times at 8, 10 and 12-d of age. During acute phase response, chicks fed dietary fish oil from 1-d of age enhanced energy balances and did not give negative effect on nitrogen balance and uric acid excretion, but elevated MnSOD and Cu/ZnSOD activity In liver and erythrocyte cytosols compared with those fed dietary soybean oil. The results indicated that enhanced daily gain and metabolizable energy value of diet in birds fed fish oil from one-d of age seems due to change on the antioxidants systems during acute phase response.

• The Korean Journal of Nuclear Medicine
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• v.32 no.1
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• pp.114-119
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• 1998

We present a case of a young female patient with fulminant hepatitis who showed an altered biodistribution of Ga-67, after being scanned twice at 10 month intervals. On initial scan, uplake of Ga-67 was increased in the liver, kidneys, and skeletons. Increased hepatic Ga-67 uptake may be explained by increased transferrin unbound Ga-67 that was taken up by the inflamed liver. The saturation of iron-binding proteins due to multiple transfusions may lead to increased renal and skeletal Ga-67 uptake. On follow-up scan hepatic Ga-67 uptake was markedly increased. Also increased Ga-67 uptake in the axial skeleton and normalized renal uptake were shown. The findings were consistent with iron deficiency anemia. This case demonstrates altered Ga-67 biodistribution associated with multiple transfusions, fulminant hepatitis, and iron deficiency anemia.

• Proceedings of the Korean Information Science Society Conference
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• 2004.04b
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• pp.307-309
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• 2004

급성 심근경색 진단용 DNA 컴퓨팅 시스템 모듈로서, 트로포닌 I (troponin I, Tnl). 트로포닌 T (troponin T, TnT). 미오글로빈 (myoglobin), C-반응 단백질 (C-reactive protein, CRP) 과 각각 결합할 수 있는 네 가지 종류의 앱타머틀 선정하고, 이의 개발을 시도하여, 그 중 첫 번째로 C-반응 단백질-결합 앱타머를 SELEX 기법을 이용하여 선별해내었다. 또한, 선별된 앱타머 염기서열에 기초하여 각각 10-mer 길이의 FDNA 와 QDNA 를 제작하고, 표적 단백질 (CRP) 과 혼합시켜 형광발현 변화의 추이를 살펴보았다. 앱타머 및 FDNA. QDNA 가 결합할 경우에는 형광감쇄효과가 발생하므로, 형광감쇄효과가 일어나지 않은 경우에 비하여 현저하게 형광측정값이 저조하게 나타나는 현상을 확인할 수 있었다. 향후 연구로, 나머지 세 가지 종류의 앱타머를 SELEX기법을 이용하여 선별해내고. 기확보된 C-반응 단백질-결합 앱타머 모듈과 함께 논리회로를 구성하는 DNA 컴퓨팅 칩을 제작할 예정이다.

• Journal of Animal Science and Technology
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• v.50 no.2
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• pp.185-198
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• 2008

Influences of dietary brown seaweed(BSW) on the nutrient metabolism, anti-oxidant enzyme activity and cell-mediated immune response were studied in broiler chicks activated acute phase response. 72 Hatched male broiler chicks(Ross) were divided into 12 pens, 6 heads per pen, and fed the BSW 0.0% (Basal) or 2.0% diet, respectively, and injected with the Salmonella typhimurium lipopoly saccharide(LPS) for activation of the acute phase response three times at 8, 10 and 12 d of age. During 4 wks of experimental feeding, growth performance of broiler chicks was not affected by dietary BSW and the acute phase response. Compared with control birds, the acute phase response did not affect the daily weight gain in birds fed BSW 2.0% diet, decreased nitrogen balance(NB) or metabolizable energy(ME) utilization per metabolic body size(kg0.75), and enhanced activities of peroxidase or extracellular SOD(EcSOD), tumor necrosis factor-alpha and ovotransferrin in plasma and MnSOD and CuZnSOD in erythrocyte cytosol. Compared to BSW 0.0% diet, 2.0% diet enhanced protein retention(NB) per kg0.75 regardless the acute phase response, did not affect uric acid nitrogen excretion(UAN) per kg0.75 in birds during the acute phase response, decreased(p<0.05) the UAN excretion per kg0.75 in control birds. And BSW 2.0% diet also decreased(p<0.05) plasma peroxide level and erythrocyte peroxidase or MnSOD activity but increased plasma peroxidase and EcSOD activity and interleukin-1 activity secreted from LPS-stimulated PBMC in 4 week broiler chicks.

• Feed Journal
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• v.4 no.10 s.38
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• pp.132-137
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• 2006

목장 경영의 성공과 실패에는 여러 가지 요인이 작용하지만 연중 얼마나 적은 소가 도태되는가 하는 것 또한 아주 중요하게 생각해 보아야 할 사항이다. 낙농에 있어 거의 대부분의 질병이 산욕기에 발병하는데 산욕기(産褥期)란 분만으로 인한 상처가 완전히 낫고, 자궁이 평상시 상태가 되며 신체의 각기관이 임신 전의 상태로 회복되기까지의 기간으로 대개 분만 후 6~8주간을 말한다. 산욕기에 주로 발생할 수 있는 다양한 질병(산욕기 부전마비, 난산, 급성유방염, 제4위 전위증 등)들이 조기에 발견되어 적절히 치료되지 못하고 만성화되면서 전해질불균형, 에너지와 단백질의 섭취부족에 의한 영양장애로 허약과 지방간 및 케토시스 등으로 진행되어 결국 도태됨으로서 목장 경영에 있어 막대한 영향을 끼치는 것이다. 이러한 산욕기 질병의 주 원인은 건유기 사양관리에 있다. 건유기간 동안 체점수(BCS)의 변화를 최대한 줄이면서 분만전의 스트레스를 얼마만큼 줄일 수 있도록 관리하는가에 달려있다. 다시말해 분만 전 마지막 한달간에 건유에서 분만으로의 이행과정을 얼마나 매끄럽게 연결 지어주는가에 따라 산욕기 질병의 발생률이 결정되는 것이다. 본 고에서는 건유기 사양관리 소홀로 발생되는 산욕기 부전마비에 관해서 자세히 설명해 보고자 한다.

• Korean Journal of Biological Psychiatry
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• v.2 no.1
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• pp.70-76
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• 1995

A few researchers have reported that major depression may be associated with higher levels of positive acute phase proteins(APPs), such as haptoglobin(Hp), ${\alpha}1$-antitrypsin(${\alpha}1AT$), ceruloplasmin(Cp) and lower levels of negative APPs(visceral proteins), such as albumin(Alb) and transferrin(Tf). Elevated levels of positive APPs and a drop in negative APPs constitute important indicators of immune activation. This study was designed to investigate whether altered serum concentrations of positive APPs and of negative APPs reflect the state of depression. Twenty patients who fulfilled DSM-III-R criteria for major depressive disorder and for dysthymic disorder and twelve normal healthy controls were included. The authors measured positive APPs(Hp, ${\alpha}1AT$, Cp) and negative APPs(Alb, Tf) using rate nephelometry and bromcresol green method. 1) There were significant increases of ${\alpha}1AT$, Cp in major depressed patients as compared with normal controls. Trends towards higher Hp and lower Alb, Tf in major depressed patients were observed. 2) No significant difference of APPs concentrations between dysthymic patients and normal controls was found. 3) Severity of depression(HDRS, BDI score) was related to Hp, Cp, ${\alpha}1AT$ value positively. Our findings are partially compatible with the hypothesis that major depression may be accompanied by acute phase response with higher levels of positive APPs and lower levels of negative APPs.

• Journal of Life Science
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• v.22 no.10
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• pp.1352-1358
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• 2012

In acute lung injury (ALI) by lipopolysaccharide (LPS), the underlying cause of infiltration and migration of neutrophils into the alveoli is considered to be from increased production of platelet-activating factor (PAF) in the pulmonary surfactant lining the alveolar lumen. In this study I partially confirmed this concept. LPS increased lung leak and the infiltration of neutrophils in the lung of rats given LPS intratracheally. The migration of neutrophils into the lung, which had caused oxidative stress, was also morphologically identified. I verified that the metabolism of the pulmonary surfactant was affected and that there was increased production of PAF in the pulmonary surfactant, both of which are considered to contribute to ALI by LPS in rats.

• Applied Microscopy
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• v.33 no.1
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• pp.1-16
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• 2003

As is well known that N-nitroso-N-methylurethane (NNNMU) causes acute lung injury (ALI) in experimental animals. And ALI caused by NNNMU is very similar to ARDS in human being in its pathology and progress. In its context, we investigated the pathogenetic mechanism of ARDS associated with oxidative stress by neutrophils in Sprague-Dawley rat model of NNNMU-induced ALI. NNNMU had increased lung weight/body weight ratio (L/B ratio), lung myeloperoxidase (MPO) activity, protein content and number of neutrophils in bronchoalveolar fluid (BALF) compared with those of control rat (p<0.001, respectively). In contrast, the amount of pulmonary surfactant in BALF was decreased by NNNMU (p<0.001). Morphologically, light microscopic examination denoted pathological findings such as formation of hyaline membrane, infiltration of neutrophils and perivascular cuffing in the lungs of NNNMU-treated rats. In addition, ultrastructural changes such as the necrosis of endothelial cells, swelling and vacuolization of lamellar bodies of alveolar type II cells, and the degeneration of pulmonary surfactant were identified after treatment of NNNMU. Very interestingly, cerium chloride electron microscopic cytochemistry showed that NNNMU had increased the production of cerrous-peroxide granules in the lung, which signified the increased production of hydrogen peroxide in the lung. Collectively, we conclude that NNNMU causes acute lung leak by the mechanism of neutrophilic oxidative stress of the lung.