• Journal of the Korean Orthopaedic Association
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• v.54 no.6
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• pp.475-477
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• 2019

Stem cell research arose from the need to explore new therapeutic possibilities for intractable and lethal diseases. Although musculoskeletal disorders are basically nonlethal, their high prevalence and the relative ease of performing clinical trials have facilitated the clinical application of stem cells in this field. On the other hand, despite the plethora of in vitro and preclinical studies in stem cell research for regenerative medicine in the musculoskeletal system, few reliable clinical studies have been published. Stem cell therapy can be applied locally for bone, cartilage, and tendon regeneration. The candidate disease modalities in bone regeneration include large bone defects, nonunion of fractures, and osteonecrosis. Focal osteochondral defect and osteoarthritis are the current targets for cartilage regeneration. For tendon regeneration, bone-tendon junction problems, such as rotator cuff tears are hot topics in clinical research. To date, the literature supporting stem cell-based therapies comprises mostly case reports or case series.

• Journal of Veterinary Clinics
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• v.24 no.2
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• pp.244-246
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• 2007

Clinical and histopathologic features of idopathic polymyositis in twenty-month-old Alaskan malamute dog are described. The clinical signs were progressive exercise intolerance with acute exacerbation of weakness, muscle atrophy, synchronous pelvic limb gait, short stiff steps and tip-toeing as like walking on eggshells. Physical and clinical examination revealed no evidence of neurologic, skeletal and secondary muscular disorders associated with other diseases. Therefore muscle biopsy was performed at the most severe muscle atrophy lesions to confirm by histopathology. Histopathologic findings documented mononuclear cell infiltration and necrosis of muscle fiber and it was diagnosed as idiopathic polymyositis. Initial treatment was focused on pain relief. Prednisone at immunosuppressive dose (2 mg/kg) was administered orally twice daily. After 3 weeks of starting treatment, the patient showed improvement of gait, appetite, exercise as well as gradually return to normal state of hematologic and serum chemistry profiles.

• The Journal of the Korean bone and joint tumor society
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• v.6 no.4
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• pp.168-172
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• 2000

Benign soft tissue mesenchymoma is a tumor composed of at least 2 mesenchymal elements, not ordinarily found together in a tumor. Very few cases are reported worldwide and even more, preponderance for cartilage in benign mesenchymoma is extremely rare. Up to our knowledge, total of 11 cases are reported to have cartilaginous rich benign soft tissue mesenchymoma. We are reporting a case of three-year-old male who developed benign soft tissue mesenchymoma rich in cartilaginous materials that occurred on the lower aspect of the back.

• Korean Journal of Poultry Science
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• v.50 no.4
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• pp.261-266
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• 2023

The skeletal muscles of livestock play a crucial role as protein sources for humans, and the consumption of poultry meat is steadily increasing worldwide. Numerous genes, including myogenic regulatory factors, are involved in myogenesis, and precise regulation of them is essential. In this study, genes specifically expressed in muscles were selected, and their promoters were cloned and analyzed. The analysis of gene expression in various tissues of animals revealed that many genes exhibited specific expression patterns in skeletal muscles, with TNNT3, TNNC2, and MYF6 genes showing similar patterns in poultry. The promoter regions of three genes were amplified by polymerase chain reaction to sizes of 1.2 kb, 1.03 kb, and 1.43 kb, respectively. These fragments were then inserted at the front of the enhanced green fluorescent protein gene in vectors. It was confirmed that the sequences of three promoters closely matched the chicken genome sequences. Upon introducing vectors with each promoter into QM7 quail muscle cells, all three promoters successfully induced the expression of the green fluorescent protein. The brightness of the green fluorescence in each promoter was approximately seven times dimmer compared to the control, CMV-IE promoter. It is predicted that more than 230 transcription factors can bind to each promoter, especially various transcription factors expressed in muscles, including myogenic regulatory factors such as MYF5, MYOD, and MYOG. These promoters can be valuable for studying gene expression in poultry muscle cells, and further research is needed to precisely investigate the regulatory region of gene expression in promoters.

• Transactions of the Korean Society of Mechanical Engineers A
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• v.34 no.6
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• pp.675-681
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• 2010

It is well known that mesenchymal stem cell(MSCs) can be differentiated into fibroblasts, chondrocytes, and osteoblasts and that they develop into fibrous tissue, cartilage, or bone, as a result of mechanical stimulation. In this study, we developed a bioreactor system, which is composed of a reactor vessel that provides the required cell culture environment, an environment controlling chamber to control the media, a gas mixer, and a reactor motion control subsystem to apply mechanical stimuli to the cells. For the MSC culture, We used a poly-urethane (PU) scaffold, with a collagen coating to ensure improved cohesion ratio. Then, we transferred the cultivated MSCs in the PU scaffold, cultured the cells in the bioreactor system, and confirmed the proliferation, differentiation, and ossification processes, resulting from mechanical stimuli.

• Journal of the Korean Society of Radiology
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• v.82 no.4
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• pp.936-942
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• 2021

Langerhans cell histiocytosis (LCH) is a rare condition that usually occurs in children and commonly affects the skeletal system. It is extremely rare in adults, especially in the clavicles. In this report, we describe a pathologically confirmed case of LCH in the clavicle of a 50-year-old male. We report various radiological findings, such as plain radiography, CT, MR, and PET-CT, along with a review of the literature.

• The Journal of the Korean bone and joint tumor society
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• v.8 no.3
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• pp.96-105
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• 2002

Purpose: The purpose of this study was to assess the oncologic results and functional outcomes of limb salvage surgery performed in patients of primary bone tumors of the shoulder girdle. Materials and Methods: Twenty-nine patients who underwent limb sparing resection for shoulder girdle neoplasm between 1982 and 2001 were analyzed. Follow up periods averaged 7 years and 1 month. Mean age of the patients was 35 (11~71) years. There were 14 males and 15 females. Primary malignant bone tumors of shoulder girdle (proximal humerus 21, scapula 3, both 1) were 23 cases; osteosarcomas 7, chondrosarcoma 14, parosteal osteosarcoma 1, hemangioendothelioma 1, and giant cell tumor of proximal humerus were 6 cases. Limb salvage surgery was performed by curettage and cementing in 7 patients, by cement molding arthroplasty in 10 patients, and by tumor prosthesis in 7 patients, by other method such as resection only, bone graft, arthrodesis in 5 patients. The Musculoskeletal Tumor Society functional rating system was used to assess functional outcomes. Results: One osteosarcoma and 2 chondrosarcoma patients died, and the survival of the salvaged limb was 88.6% at the final follow-up. There were 6 local recurrences, 2 lung metastases, 2 local recurrences and lung metastases. The functional outcome was 80%. There was statistically significant difference of functional results among the patients treated by cement filling (86%), cement molding arthroplasty and IM nailing (71%), and tumor prosthesis (83%). (p=0.034) There were three complications including 1 radial nerve palsy and 1 axillary nerve palsy, and 1 wound infection. Dislodgement of vascularized fibular graft in one patient was treated by internal fixation. Conclusion: Limb salvage surgery seems to be useful method to treat bone tumors of the shoulder girdle.

• Journal of the Korean Society for Precision Engineering
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• v.26 no.3
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• pp.137-143
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• 2009

It has been reported that mechanical stimulation takes a role in improving eel/ growth in skeletal system. Various research groups have been showed their own bioreactors which stimulate cell-seed three-dimensional scaffold. In this study, we hypothesized that the various conditions of mechanical stimulation would affect cell growth and proliferation. To prove our hypothesis, we designed a custom-made bioreactor capable of applying controlled compression to cell-encapsulated scaffolds. This device consisted of a circulation system and a compression system. Each parts of the bioreactor was fabricated using the rapid prototyping technology By using the rapid prototyping technology, we can modify and improve the bioreactor very rapidly For dynamic cell-culture, cell-encapsulated agarose gel was fabricated in 2% concentration. We performed dynamic cell-culture using this agarose gel and developed bioreactor in 3 days.

• The Journal of the Korean bone and joint tumor society
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• v.19 no.1
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• pp.28-32
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• 2013

Erdheim Chester disease (ECD) is very rare non-Langerhans cell histiocytosis (LCH) which occurs in the skeletal system and multiple organs. As it is progressive, sometimes it causes fatal results. However, it is often misdiagnosed as LCH or multiple bone metastasis and, thus, is very difficult to diagnose. In Korea, only 10 cases were first reported in 1999. In particular, there have been a few orthopedic approaches or reports in English-speaking literatures, and no report has been issued in Korea. The authors performed bone biopsy in patients with knee and lower extremity pain who were referred for the integrated treatment. We attempts to report this diagnosis experience with literature review.

• Proceedings of the Korea Contents Association Conference
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• 2013.05a
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• pp.383-384
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• 2013

본 연구는 스포츠용 탄력 압박 스타킹(Essential compression calf sleeve, SKINS, Australia)을 착용하고 최대 운동을 하였을 때, 하지 부종에 어떠한 효과가 있는지를 알아보고자 한다. 실험 대상자는 젊은 성인 남녀 30명으로(남자 15명, 여자15명) 근 골격계 및 심혈관계에 특이 병력이나 사고에 의한 외상 등이 없었으며, 규칙적으로 운동을 하는 자는 제외하였다. 대상자들의 신체적 특성을 파악하기 위해서 자동 신장계(BSM330, Biospace, Korea)를 사용하였다. 부종정도를 알기위해서 줄자로 운동 전후의 장딴지 근(종아리)의 둘레와 체성분 분석기(InBody 720, Biospace, Korea)를 사용하여 하지의 부종 수치를 측정하였다. 최대의 운동은 호흡가스 분석기와 연결된 트레이드밀(CPEX-1, IRS, Japan)을 사용하여 변형된 BRUCE Protocol TR-3에 따라 호흡 교환율(R) 값이 1.15이상 되거나 all-out 될 때까지 하였다. 실험 대상자들은 동일 집단으로 전이효과를 방지하기 위하여 일주일의 간격을 두고 스포츠 탄력 압박 스타킹 착용 유무에 따른 동일한 실험을 두 번 하였다. 측정은 최대 운동 전후의 장딴지 근(종아리)의 둘레와 부종 수치를 하였다. 스포츠용 탄력 압박 스타킹 착용 유무와 운동 전후를 비교하기 위하여 MANOVA(다변량 분산분석)를 사용하였고 유의수준은 .05로 설정하였다. 탄력 압박 스타킹을 착용한 상태에서 최대 운동 전후의 부종 정도를 측정한 결과 스타킹을 착용하지 않았을 때 운동 전후의 둘레가 유의하게 증가하였고(p<0.05), 스타킹을 착용 하였을 때 운동 전후의 둘레가 유의하게 증가하지 않았다(p>0.05). 부종 수치(세포외액/전체체액)는 탄력 압박 스타킹을 착용하였을 때 오른쪽 다리는 유의하지 않게 감소하였고(p>0.05), 왼쪽 다리는 유의하게 감소하였다(p>0.05). 탄력 압박 스타킹을 착용하고 운동할 경우 하지의 부종 경감에 어느 정도 효과가 있다고 사료된다.