Browse > Article

Development of Bioreactor by Rapid Prototyping Technology  

Park, Jeong-Hun (Department of Precision Mechanical Engineering, Chungbuk National Univ.)
Lee, Seung-Jae (BK21 Mechatronics groups, Chungnam National Univ.)
Lee, In-Hwan (School of Mechanical Engineering, Chungbuk National Univ.)
Cho, Dong-Woo (Department of Mechanical Engineering, POSTECH)
Rhie, Jong-Won (Department of Plastic Surgery, College of medicine, Catholic Univ.)
Publication Information
Journal of the Korean Society for Precision Engineering / v.26, no.3, 2009 , pp. 137-143 More about this Journal
Abstract
It has been reported that mechanical stimulation takes a role in improving eel/ growth in skeletal system. Various research groups have been showed their own bioreactors which stimulate cell-seed three-dimensional scaffold. In this study, we hypothesized that the various conditions of mechanical stimulation would affect cell growth and proliferation. To prove our hypothesis, we designed a custom-made bioreactor capable of applying controlled compression to cell-encapsulated scaffolds. This device consisted of a circulation system and a compression system. Each parts of the bioreactor was fabricated using the rapid prototyping technology By using the rapid prototyping technology, we can modify and improve the bioreactor very rapidly For dynamic cell-culture, cell-encapsulated agarose gel was fabricated in 2% concentration. We performed dynamic cell-culture using this agarose gel and developed bioreactor in 3 days.
Keywords
Bioreactor; Mechanical Stimulation; Rapid Prototyping Technology; Musculoskeletal Cell; Agarose Gel;
Citations & Related Records
Times Cited By KSCI : 1  (Citation Analysis)
연도 인용수 순위
1 Knight, M. M., Ghori, S. A., Lee, D. A. and Bader, D. L., "Measurement of the deformation of isolated chondrocytes in agarose subjected to cyclic compression," Medical Engineering & Physics, Vol. 20, No. 9, pp. 684-688, 1998   DOI   ScienceOn
2 Mauck, R. L., Soltz, M. A., Wang, C. C., Wong, D. D., Chao, P. H., Valhmu, W. B., Hung, C. T. and Ateshian, G. A., "Functional Tissue Engineering of Articular Cartilage Through Dynamic Loading of Chondrocyte-Seeded Agarose Gels," Journal of Biochemical Engineering, Vol. 122, Issue 3, pp. 252-260, 2000   DOI   ScienceOn
3 Meyer, U., Buchter, A., Nazer, N. and Wiesmann, H. P., "Design and performance of a bioreactor system for mechanically promoted three-dimensional tissue engineering," British Journal of Oral and Maxillofacial Surgery, Vol. 44, No. 2, pp. 134-140, 2006   DOI   ScienceOn
4 Khang, G., Kim, M. S., Min, B. H., Lee, I., Rhee, J. M. and Lee, H. B., "Scaffold for Tissue Engineering," Tissue Engineering and Regenerative Medicine, Vol. 3, No. 4, pp. 376-395, 2006
5 Lee, S. J. and Cho, D. W., "Solid Freeform Fabrication Technique in Tissue Engineering," Journal of the Korean Society for Precision Engineering, Vol. 23, No. 12, pp. 7-15, 2006   과학기술학회마을   ScienceOn
6 Awad, H. A., Wickkham, M. Q., Leddy, H. A., Gimble, J. M. and Guilak, F., "Chondrogenic differentiation of adipose-derived adult stem cells in agarose, alginate, and gelatin scaffolds," Biomaterials, Vol. 25, Issue 16, pp. 3211-3222, 2004   DOI   ScienceOn
7 Nugent-Derfus, G. E., Takara, T., O'Neill, J. K., Cahill, S. B., Gortz, S., Pong, T., Inoue, H., Aneloski, N. M., Wang, W. W., Vega, K. I., Klein, T. J., Hsieh-Bonassera, N. D., Bae, W. C., Burke, J. D., Bugbee, W. D. and Sah, R. L., "Continuous passive motion appilied to whole joints stimulates chondrocyte biosynthesis of PRG41," OsteoArthritis and Cartilage, Vol. 15, Issue 5, pp. 566-574, 2007   DOI   ScienceOn
8 Kisiday, J. D., Jin, M., DiMicco, M. A., Kurz, B. and Grodzinsky, A. J., "Effects of dynamic compressive loading on chondrocyte biosynthesis in selfassemblingpe ptide scaffolds," Journal of Biomechanics, Vol. 37, Issue 5, pp. 594 -604, 2004