• Title/Summary/Keyword: 교차조사

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Serological Diagnosis of Human Sparganosis by means of micro-ELISA (효소면역측정법을 이용한 스파르가눔증의 혈청학적 진단)

  • Hyuck Kim;Suk-Il Kim;Seung-Yull Cho
    • Parasites, Hosts and Diseases
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    • v.22 no.2
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    • pp.223-228
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    • 1984
  • Seven cases of surgically proven sparganosis were serologically tested by means of microELISA for their specific IgG antibody levels. For that purpose, crude saline extract of spargana from snake, Natrix tigrina lateralis was prepared and used as antigen. The sparganosis sera were also tested with Paragonimus and Cysticercus antigens to observe the cross reactivity. A total of 71 sera from normal control, ectopic and pulmonary paragonimiasis, clonorchiasis, cysticerCOSIS and Taenia saginata cases were also included. Except for one case of old calcified infection, all of 6 human sparganosis showed higher serum levels of specific IgG antibody when the differential point of positive reaction was set at the absorbance value of 0.25 (the sensitivity being 85.7%). In control and other helminthic infections, all except 3 cases of T. saginata infection showed negative reaction to sparganum antigen (the specificity being 90.7%). None of sparganosis cases showed cress reactivity to Paragonimus and Cysticercus antigens. Undiluted cerebrospinal l1uid also showed high levels of antibody when central nervous system was invaded. The serologic diagnosis by means of micro ELISA could be a useful tool in epidemiological study of human sparganosis in susceptible population, as well as in individual diagnosis.

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Complement-dependent Cytotoxic Crossmatch for Prevention of Hyperacute Rejection in Canine Renal Allografts (개의 신장 동종 이식에서 초급성 거부반응의 예방을 위한 보체의존성 세포독성 DLA 플래스 I 교차 반응)

  • Ghil Heh Myung;Woo Heung-Myung
    • Journal of Veterinary Clinics
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    • v.22 no.4
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    • pp.322-327
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    • 2005
  • DLA class I complement-dependent cytotoxicity (CDC) cross-match method was established to control hyperacute rejections in organ transplantation. The aim of the present study is to investigate if DLA class 1 CDC corssmatch method is effective to prevent hyperacute rejections in canine renal allografts. Seven mongrel dogs of similar age and weight were used. Erythrocyte crossmatch was first performed and only the negatives were used. Among the same blood types, CDC cross-match was performed. Anti-dog serum, Hank's balanced salt solution(HBSS), and tile self-serum was used as a positive-, a negative-, and all auto-control respectively. After the reaction with class I complement, it was stained with eosin and scored by international cytotoxicity scoring system under inverted phase microscope. According to these results, kidneys oi CDC negatives among same blood types were cross-transplanted to observe the incidence of hyperacute rejections. One of four 1.2 B blood type dogs had autoantibodies. here were negative CDC results among each blood type, and also there were negative results between different blood types. Two pairs with the same blood types and negative CDC results underwent allo-transplantation each other. There were no hyperacute rejections. DLA cross-match method studied in this experiment for canine renal allograft can be effective to prevent hyperacute rejections. it may be applicable for the future studies of histocompatibility testing in canine renal allografts.

Measurement of Tumor Dose Using Optically Stimulated Luminescence Detectors (OSLDs) and Ionization Chambers for Primary and Metastatic Lymph Node Cancers with Head and Neck: Comparison of Beam Spoiler and Bolus (광자극발광선량계와 이온함을 이용한 두경부 원발종양 및 림프절 전이성 종양의 선량 측정: 산란판과 볼루스의 비교)

  • Lee, Jeong-Ok;Lee, Jae-Seung;Jeong, Dong-Hyeok
    • Journal of Radiation Protection and Research
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    • v.36 no.3
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    • pp.160-167
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    • 2011
  • This study conducts cross-comparison through verification of treatment planning of using beam spoiler and bolus, according to the dose variation of different tumor bed and metastatic lymph node cancers, against ionization and optically stimulated luminescence detectors(OSLDs), in head and neck radiotherapy. Verification of treatment planning examined the feasibility of inserting detectors through simulated solid dry water slabs under identical irradiated conditions from treatment planning system to measure beam spoiler and 0.5, 1 cm bolus. In addition, two detectors were cross-compared for verification of treatment planning accuracy and reliability within ${\pm}$2%. The study found that, given a beam spoiler thickness of 0.5 cm and beam spoiler-to-skin distance of 10 cm subjected to optimal dose distribution given for metastatic lymph node cancers, the bolus low-level skin dose was less, and the tumor bed dose reduced slightly. Additionally, two detectors were cross-compared for accuracy within ${\pm}$1%. Accordingly, The use of beam spoiler was determined that reduces skin side effects and can deliver an optimal dose distribution for tumor, and to apply to future clinical studies should be performed.

Detection of Peanuts in Commercially Processed Foods by an Enzyme-Linked Fluorescent Immunoassay (Enzyme-linked fluorescent immunoassay에 의한 가공식품 중 땅콩의 검출)

  • Kim, Mi-Hye;Kim, Hyun-Jung;Shon, Dong-Hwa
    • Korean Journal of Food Science and Technology
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    • v.41 no.1
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    • pp.111-115
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    • 2009
  • In this study we analysed for peanuts in processed foods using an enzyme-linked fluorescent immunoassay (ELFA), and compared the results with labeled ingredients. Crude peanut protein (CPP) was immunized into rabbits to produce specific antibodies(Ab). A sandwich ELFA was established using anti-CPP Ab and Ab-horseradish peroxidase (HRP) conjugate. The cross-reactivities of the Ab toward CPP, peanuts, almonds, soybeans, and walnuts were 100, 9.8, $1.1{\times}10^{-2},\;4.4{\times}10^{-3}$, and 0%, respectively. The samples included 19 items consisting of biscuits, snacks, chocolates, and so on. The results from the sandwich ELFA showed that peanuts were contained in 7 of the processed food items, among which, 5 items were labeled as having peanuts present but 2 items were not. One of the 2 items that was peanut-detected but unlabeled was a biscuit labeled to contain almonds and assayed to contain $2.1{\times}10^{-3}%$ peanuts, which might have been due to the weak cross-reactivity of the Ab toward almonds. The other item was a snack labeled to contain soybeans and assayed to contain 0.098% peanuts, which might have been due to peanut cross-contamination during processing, since the crossreactivity of the Ab toward soybeans was very weak. These results suggest that ELFA is a good tool to detect peanuts in processed foods, and allergens in certain processed foods should be labeled correctly.

Development of a Traffic Accident Prediction Model for Urban Signalized Intersections (도시부 신호교차로 안전성 향상을 위한 사고예측모형 개발)

  • Park, Jun-Tae;Lee, Soo-Beom;Kim, Jang-Wook;Lee, Dong-Min
    • Journal of Korean Society of Transportation
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    • v.26 no.4
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    • pp.99-110
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    • 2008
  • It is commonly estimated that there is a much higher potential for accidents at a crossroads than along a single road due to its plethora of conflicting points. According to the 2006 figures by the National Police Agency, the number of traffic accidents at crossroads is greatly increasing compared to that along single roads. Among others, crossroads installed with traffic signals have more varied influential factors for traffic accidents and leave much more room for improvement than ones without traffic signals; thus, it is expected that a noticeable effect could be achieved in safety if proper counter-measures against the hazards at a crossroads were taken together with an estimate of causes for accidents This research managed to develop models for accident forecasts and accident intensity by applying data on accident history and site inspection of crossroads, targeting four selected downtown crossroads installed with traffic signals. The research was done by roughly dividing the process into four stages: first, analyze the accident model examined before; second, select variables affecting traffic accidents; third, develop a model for traffic accident forecasting by using a statistics-based methodology; and fourth, carry out the verification process of the models.

Determination of Hydraulic Conductivities in the Sandy Soil Layer through Cross Correlation Analysis between Rainfall and Groundwater Level (강우-지하수위 상관성 분석을 통한 사질토층의 수리전도도 산정)

  • Park, Seunghyuk;Son, Doo Gie;Jeong, Gyo-Cheol
    • The Journal of Engineering Geology
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    • v.29 no.3
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    • pp.303-314
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    • 2019
  • Surface permeability and shallow geological structures play significant roles in shaping the groundwater recharge of shallow aquifers. Surface permeability can be characterized by two concepts, intrinsic permeability and hydraulic conductivity, with the latter obtained from previous near-surface geological investigations. Here we propose a hydraulic equation via the cross-correlation analysis of the rainfall-groundwater levels using a regression equation that is based on the cross-correlation between the grain size distribution curve for unconsolidated sediments and the rainfall-groundwater levels measured in the Gyeongju area, Korea, and discuss its application by comparing these results to field-based aquifer test results. The maximum cross-correlation equation between the hydraulic conductivity derived from Zunker's observation equation in a sandy alluvial aquifer and the rainfall-groundwater levels increases as a natural logarithmic function with high correlation coefficients (0.95). A 2.83% difference between the field-based aquifer test and root mean square error is observed when this regression equation is applied to the other observation wells. Therefore, rainfall-groundwater level monitoring data as well as aquifer test are very useful in estimating hydraulic conductivity.

A Convergence Study on association of Internet Use Time with Perceived Status in Adolescents (청소년 인터넷 사용시간이 청소년 주관적 상태에 미치는 영향에 대한 융합연구)

  • Baek, Seung Hee;Kim, Ji hyun
    • Journal of the Korea Convergence Society
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    • v.9 no.11
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    • pp.153-159
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    • 2018
  • The purpose of this study is to grasp the internet use time that young people use for purposes other than learning purpose, to grasp the perceived status of the youth according to internet use time and to grasp the interrelationships of them. Using the 2016 youth health behavior online survey, the odds ratios and 95% confidence intervals of perceived status according to internet use time were calculated by binary logistic regression analysis. The main results are as follows. In perceived health and perceived oral health the odds ratios of perceived who feel that they are perceived and unhealthy as the time spent using the Internet increased significantly compared to those who did not use the Internet for learning purposes. In the perceived body type, the odds ratio of being overweight increased significantly with longer internet use time. The odds ratios of perceived happiness were 1.19 times (CI = 1.10-1.30) higher than the perceived expectation of unhappiness when using the Internet for over 300 minutes. The use of the internet for a long time other than the purpose of learning may have a negative effect on the health and happiness of the youth, so we think that the recommended time for using the internet is necessary.

Longitudinal Relationship between Negative Parenting Attitudes Perceived by Adolescents, Self-Esteem, and Life Satisfaction: Application of the Autoregressive Cross-Lagged Model (청소년이 인식한 부모의 부정적 양육태도와 자아존중감, 삶의 만족도 간의 종단적 관계: 자기회귀교차지연모형의 적용)

  • Lee, Seoyeon
    • Journal of Korean Home Economics Education Association
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    • v.34 no.3
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    • pp.49-65
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    • 2022
  • This study aims to examine the mutual influences of negative parenting attitudes, self-esteem, and life satisfaction perceived by adolescent children through longitudinal perspective. Data from the first to third years of the panel of the first year middle school students were used among the 2018 data of the Korean Children and Youth Panel Survey (KCYPS) of the Korea Youth Policy Institute. The longitudinal correlation was analyzed by applying the autoregressive cross-lagged model which can determine the direction of longitudinal causality between variables. As a result of the analysis, it was found that the negative parenting attitudes, self-esteem, and life satisfaction perceived by adolescents had a positive effect on the negative parenting attitude perception, self-esteem, and life satisfaction at later times, respectively. In addition, the higher the negative parenting attitude at the previous point was, the lower the self-esteem or life satisfaction afterward. The higher the life satisfaction, the lower the negative parenting attitude of the parents, and the higher the self-esteem. Recognition of negative parenting attitudes of parents lowered the self-esteem of adolescents, whereas the cross-delay coefficient from self-esteem to negative parenting attitude perception was not significant. Through these results, the longitudinal relationship between negative parenting attitude perception of adolescence, self-esteem, and life satisfaction was confirmed and efforts to form positive self-esteem and improve life satisfaction were discussed.

Cross-resistance of Colletotrichum acutatum s. lat. to Strobilurin Fungicides and Inhibitory Effect of Fungicides with Other Mechanisms on C. acutatum s. lat. Resistant to Pyraclostrobin (Strobilurin계 살균제에 대한 고추탄저병균의 교차저항성과 Pyraclostrobin 저항성균에 대한 다른 기작 살균제의 억제 효과)

  • Park, Subin;Kim, Heung Tae
    • Research in Plant Disease
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    • v.28 no.3
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    • pp.122-131
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    • 2022
  • Colletotrichum acutatum s. lat. 20JDS8 sensitive and 20CDJ6 resistant to pylaclostrobin were used to investigate the cross-resistance with fungicides belonging to the strobilurins and the characteristics of fungicidal controlling activities with different mechanisms against the isolate resistant to the fungicide. The resistant isolate of 20CDJ6 also showed the resistance to azoxystrobin, trifloxystrobin, and kresoxim-methyl, suggesting that there is a cross-resistance relationship. All fungicides with different action mechanisms inhibited mycelial growth of both susceptible and resistant isolates of C. acutatum s. lat., but their disease control effects in fruits were different according to the fungicides. The disease control effect of isopyrazam against 20JDS8 and 20CDJ6 was very low, and fluazinam showed a control effect of 91.9% and 88.1% against 20JDS8 and 20CDJ6 only when it was treated before inoculation by spraying spore suspensions on pepper fruits without wounds. Tebuconazole and prochloraz effectively inhibited not only the mycelial growth of 20JDS8 and 20CDJ6 on potato dextrose agar medium, but also disease incidence in red pepper fruits. As a result of this study, C. acutatum s. lat. 20CDJ6 resistant to pyraclostrobin showed cross-resistance with other strobilurin fungicides. In addition, we think that fluazinam, tebuconazole, and prochloraz can be recommended as alternative fungicides for the control of red-pepper pyranthracnose pathogens resistant pyraclostrobin. However, fluazinam can be effective only if it is treated protectively before the occurrence of the disease.

The Longitudinal Effect between Disability Acceptance and Job Satisfaction of Wage Working Disabled Persons: Adapting the Autoregressive Cross-Lagged Model and Multigroup Analysis

  • Lee, Hyoung-Ha
    • Journal of the Korea Society of Computer and Information
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    • v.27 no.9
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    • pp.177-190
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    • 2022
  • In this study, using data from the second wave of Panel Survey of Employment for The Disabled[PESD] (2016) to the sixth year (2021), the longitudinal changes between disability acceptance and job satisfaction of adult wage workers aged 20 or older with disabilities and the correlation An autoregressive cross-lagged model was applied to analyze the causal relationship. As a result of the analysis, first, the disability acceptance and job satisfaction at the previous time point of the disabled workers were stably significant to the disability acceptance(𝛽=.556~.610) and job satisfaction(𝛽=.554~.585) at the later time point. Second, disability acceptance at the previous point in time for wage workers with disabilities crosses the job satisfaction at the later time point(𝛽=.077~.090), and the job satisfaction at the previous point crosses the disability acceptance at the later time point(𝛽=.087~.092). Third, as a result of model fit analysis according to the gender group of wage workers with disabilities, the difference in the autoregressive effect between disability acceptance and job satisfaction and the cross-lagged effect between disability acceptance and job satisfaction according to the passage of time was not significant.