Browse > Article
http://dx.doi.org/10.5423/RPD.2022.28.3.122

Cross-resistance of Colletotrichum acutatum s. lat. to Strobilurin Fungicides and Inhibitory Effect of Fungicides with Other Mechanisms on C. acutatum s. lat. Resistant to Pyraclostrobin  

Park, Subin (Department of Plant Medicine, Chungbuk National University)
Kim, Heung Tae (Department of Plant Medicine, Chungbuk National University)
Publication Information
Research in Plant Disease / v.28, no.3, 2022 , pp. 122-131 More about this Journal
Abstract
Colletotrichum acutatum s. lat. 20JDS8 sensitive and 20CDJ6 resistant to pylaclostrobin were used to investigate the cross-resistance with fungicides belonging to the strobilurins and the characteristics of fungicidal controlling activities with different mechanisms against the isolate resistant to the fungicide. The resistant isolate of 20CDJ6 also showed the resistance to azoxystrobin, trifloxystrobin, and kresoxim-methyl, suggesting that there is a cross-resistance relationship. All fungicides with different action mechanisms inhibited mycelial growth of both susceptible and resistant isolates of C. acutatum s. lat., but their disease control effects in fruits were different according to the fungicides. The disease control effect of isopyrazam against 20JDS8 and 20CDJ6 was very low, and fluazinam showed a control effect of 91.9% and 88.1% against 20JDS8 and 20CDJ6 only when it was treated before inoculation by spraying spore suspensions on pepper fruits without wounds. Tebuconazole and prochloraz effectively inhibited not only the mycelial growth of 20JDS8 and 20CDJ6 on potato dextrose agar medium, but also disease incidence in red pepper fruits. As a result of this study, C. acutatum s. lat. 20CDJ6 resistant to pyraclostrobin showed cross-resistance with other strobilurin fungicides. In addition, we think that fluazinam, tebuconazole, and prochloraz can be recommended as alternative fungicides for the control of red-pepper pyranthracnose pathogens resistant pyraclostrobin. However, fluazinam can be effective only if it is treated protectively before the occurrence of the disease.
Keywords
Cross resistance; Fungicidal activity; Pyraclostrobin; Red-pepper;
Citations & Related Records
Times Cited By KSCI : 1  (Citation Analysis)
연도 인용수 순위
1 Ishii, H., Fraaije, B. A., Sugiyama, T., Noguchi, K., Nishimura, K., Takeda, T. et al. 2001. Occurrence and molecular characterization of strobilurin resistance in cucumber powdery mildew and downy mildew. Phytopathology 91: 1166-1171.   DOI
2 Kim, Y.-S., Dixon, E. W., Vincelli, P. and Farman, M. L. 2003. Field resistance to strobilurin (QoI) fungicides in Pyricularia grisea caused by mutations in the mitochondrial cytochrome b gene. Phytopathology 93: 891-900.   DOI
3 Kim, J. T., Lee, K. H., Min, J. Y., Cho, I. J., Kang B. K., Park S. W. et al. 2004. Fluctuation of the sensitivity of Colletotrichum spp. causing the red-pepper anthracnose to chlorothalonil. Korean J. Pestic. Sci. 8: 231-237. (In Korean)
4 Kim, J. T., Park, S.-Y., Choi, W., Lee, Y.-H. and Kim, H. T. 2008. Characterization of Colletotrichum isolates causing anthracnose of pepper in Korea. Plant Pathol. J. 24: 17-23.   DOI
5 Kim, A. H., Kim, S. B., Han, K. D. and Kim, H. T. 2014. Monitoring for the resistance of strobilurin fungicide against Botrytis cinerea causing gray mold disease. Korean J. Pestic. Sci. 18: 161-167. (In Korean)   DOI
6 Kim, S., Min, J. and Kim, H. T. 2019. Occurrence and mechanism of fungicide resistance in Colletotrichum acutatum causing pepper anthracnose against pyraclostrobin. Korean J. Pestic. Sci. 23: 202-211. (In Korean)   DOI
7 Rural Development Administration. 2022. Pesticide safety information system. URL https://psis.rda.go.kr/psis/agc/res/agchmRegistStusLst.ps [1 September 2022].
8 Sierotzki, H., Wullschleger, J. and Gisi, U. 2000. Point mutation in cytochrome b gene conferring resistance to strobilurin fungicides in Erysiphe graminis f. sp. tritici field isolates. Pestic. Biochem. Physiol. 68: 107-112.   DOI
9 Tudi, M., Ruan, H. D., Wang, L., Lyu, J., Sadler, R, Connell, D. et al. 2021. Agriculture development, pesticide application and its impact on the environment. Int. J. Environ. Res. Public Health 18: 1112.   DOI
10 Vitoratos, A. G. 2014. Mode of action and genetic analysis of resistance to fluazinam in Ustilago maydis. J. Phytopathol. 162: 737-746.   DOI
11 Wood, P. N. and Fisher, B. M. 2017. The effect of fungicides on spore germination, mycelial growth and lesion development of Phlyctema vagabunda (syn: Neofabraea alba) (bull's eye rot of apples). N. Z. Plant Prot. 70: 112-119.
12 Gisi, U., Sierotzki, H., Cook, A. and McCaffery, A. 2002. Mechanisms influencing the evolution of resistance to Qo inhibitor fungicides. Pest. Manag. Sci. 58: 859-867.   DOI
13 Steinfeld, U., Sierotzki, H., Parisi, S. Poirey, S. and Gisi, U. 2001. Sensitivity of mitochondrial respiration to different inhibitors in Venturia inaequalis. Pest. Manag. Sci. 57: 787-796.   DOI
14 Li, T., Xiu, Q., Zhang, J., Wang, J. X., Duan, Y. B. and Zhou, M. G. 2020. Pharmacological characteristics and efficacy of fluazinam against Corynespora cassiicola, causing cucumber target spot in greenhouses. Plant Dis. 104: 2449-2454.   DOI
15 Ma, Z., Felts, D. and Michailides, T. J. 2003. Resistance to azoxystrobin in Alternaria isolates from pistachio in California. Pestic. Biochem. Physiol. 77: 66-74.   DOI
16 Harp, T. L., Godwin, J. R., Scalliet, G., Walter, H., Stalker, A. D., Bartlett, D. W. et al. 2011. Isopyrazam, a new generation cereal fungicide. Asp. Appl. Biol. 106: 113-120.
17 Heaney, S. P., Hall, A. A., Davies, S. A. and Olaya, G. 2000. Resistance to fungicide in the QoI-STAR cross-resistance group: current perspective. In: Proceeding of the British Crop Protection Conference: Pest and Disease, Vol. 2, pp. 755-762. British Crop Protection Council, Franham, Surrey, UK.
18 Bartlett, D. W., Clough, J. M., Godwin, J. R., Hall, A. A., Hamer, M. and Parr-Dobrzanski, B. 2002. The strobilurin fungicides. Pest. Manag. Sci. 58: 649-662.   DOI
19 Collina, M., Landi, L., Guerrini, P., Branzanti, M. B. and Brunelli, A. 2005. QoI resistance of Plasmopara viticola in Italy: biological and quantitative real-time PCR approaches. In: Modern Fungicides and Antifungal Compounds, IV, eds. by H. W. Dehne, U. Gisi, K. H. Kuck, P. E. Russell and H. Lyr, pp. 81-88. BCPC, Alton, Hants, UK.
20 Isa, D. A., Ahn, S., Park, S. and Kim, H. T. 2020. Monitoring of resistance of Colletotrichum acutatum causing pepper anthracnose to pyraclostrobin using quantitative sequencing and agar dilution method. In: The 2020 KSPP Conference & Special Symposium, The International Year of Plant Health in 2020, p. 83. Korean Society of Platn Pathology, Seoul, Korea.