• Title/Summary/Keyword: 교잡육종

Search Result 191, Processing Time 0.023 seconds

PCR-based markers to select plastid genotypes of Solanum acaule (Solanum acaule 색소체 유전자형 선발을 위한 특이적 분자마커 개발)

  • Park, Tae-Ho
    • Journal of Plant Biotechnology
    • /
    • v.49 no.3
    • /
    • pp.178-186
    • /
    • 2022
  • The tetraploid Solanum acaule is a wild potato species from Bolivia widely used for potato breeding because of its diverse attractive traits, including resistance to frost, late blight, potato virus X, potato virus Y, potato leafroll virus, potato spindle tuber viroid, and cyst nematode. However, the introgression of useful traits into cultivated potatoes via crossing has been limited by differences in endosperm balance number between species. Somatic fusion could be used to overcome sexual reproduction barriers and the development of molecular markers is essential to select proper fusion products. The chloroplast genome of S. acaule was sequenced using next-generation sequencing technology and specific markers for S. acaule were developed by comparing the obtained sequence with those of seven other Solanum species. The total length of the chloroplast genome is 155,570 bp, and 158 genes were annotated. Structure and gene content were very similar to other Solanum species and maximum likelihood phylogenetic analysis with 12 other species belonging to the Solanaceae family revealed that S. acaule is very closely related to other Solanum species. Sequence alignment with the chloroplast genome of seven other Solanum species revealed four InDels and 79 SNPs specific to S. acaule. Based on these InDel and SNP regions, one SCAR marker and one CAPS marker were developed to discriminate S. acaule from other Solanum species. These results will aid in exploring evolutionary aspects of Solanum species and accelerating potato breeding using S. acaule.

'Choha', an Early-maturing and High-quality Apricot (식미 우수한 조생종 살구 '초하(初夏)')

  • Kang, Sang-Jo;Chung, Kyeong-Ho;Kim, Ho-Yeol;Jun, Ji-Hyae;Lee, Don-Kyun
    • Horticultural Science & Technology
    • /
    • v.17 no.1
    • /
    • pp.21-22
    • /
    • 1999
  • 'Choha' was released by the National Horticultural Research Institute in 1997 as a new early-maturing and high-quality apricot variety, which originated from the crossing of 'N.Y. 472' and 'Early Orange' in 1986. This variety was tested as 'Wonkyo Sa-05' at 4 areas for 3 years from 1995. 'Choha' is resistant to fruit cracking by rainy spells just before fruit maturing. Fruits mature in late June, earlier than any other varieties. The content of soluble solids is $12.5^oBx$ and fruit acidity is low. Fruits are relatively large, about 60g in weight.

  • PDF

Breeding of a Seedless Table Grape Cultivar 'Heukisul' (Vitis sp.) with High Quality (고품질 무핵 포도 품종 '흑이슬' 육성)

  • Park, Sung-Min
    • Horticultural Science & Technology
    • /
    • v.29 no.5
    • /
    • pp.507-509
    • /
    • 2011
  • 'Heukisul' (3x) a cross made in 2000 between 'Kyoho' (4x, Vitis sp.) and 'Thompson Seedless' (2x, Vitis vinifera L.) in a seedless grape breeding program, was preliminarily selected in 2004. After regional adaptation testing under the name of 'KTS014' at two sites during 2004-2005, it was finally selected in 2006. 'Heukisul' showed low incidence of berry shattering, resulting in a good berry set compared to 'King Dela'. 'Heukisul' had budburst on April 23, flowering on in June 9, and fruit maturation in September 29 (almost the same as 'King Dela' at Chuncheon), and it was considered a midseason cultivar. The mean berry weight was 4.0 g, about 0.7 g heavier than 'King Dela', and mean soluble solids were $21.9\;^{\circ}Brix$, about $2.7\;^{\circ}Brix$ higher than 'King Dela'. The skin color was dark violet with abundant bloom and the flesh was very firm. Although the cluster was compact, it required no cluster thinning. Also the incidence of berry cracking was very low.

Cultivation method and characterization of commercial strain 'Jinhwang' in Pholiota adiposa (검은비늘버섯 품종 '진황'의 특성 및 재배법)

  • Kong, Won-Sik;Yoo, Young-Bok;Jhune, Chang-Sung;You, Chang-Hyun;Cho, Yong-Hyun;Chang, Who-Bong;Kim, Kwang-Ho
    • Journal of Mushroom
    • /
    • v.4 no.3
    • /
    • pp.106-110
    • /
    • 2006
  • A new commercial strain 'Jinhwang' of Pholiota adiposa was developed by hyphal anastomosis. It was developed with crossing between monokaryotic strains derived from ASI 24001 and ASI 24004. The optimum temperature of mycelial growth was $25^{\circ}C$ and that of fruiting body development was $15{\sim}18^{\circ}C$. The color of pileus was yellowish brown and many scales and gelatinous sheath were on cap. Average number of individuals a bottle was 26 and its stipe was thick. P. adiposa can be produced by box cultivation using cotton waste.

  • PDF

Some Facts in the Course of the Segregation and Selection of the Sex-limited Inheritance Character of Silkworm Larval Marking (한성형잠 분리 선발 과정에서의 몇 가지 사실에 관하여)

  • 이상풍;홍기원;김계명
    • Journal of Sericultural and Entomological Science
    • /
    • v.17 no.1
    • /
    • pp.47-53
    • /
    • 1975
  • This experiment was carried out to obtain a marked larvae in sex-limited inheritance, using F$_1$ hybrid Bo ok X Chun san introduced from Japan. Sequence of backcrossing has been done through the earlier generation with a recessive character of plain marked larvae. 1. It is noted that genotypic segregation of sex-limited larval marking was observed in F$_4$ generation; female possesses larval marking and male shows a plain marking. 2. Larval marking heredity follows a diagram of segregation with an expected genotype such as ♀ : (W$.$+p)/Z$.$P/P and ♂ : Z/Z$.$P/P. 3. It is observed that dissociation was occurred to produce female with a genotype of W/Z$.$P/P in segregation ana selection. 4. Abnormal ratio of sexuality is observed in the course of segregation and segregation and analysis of it is continuously under way. 5, It is observed that the difference of qualitative characteristics between female and male obtained from the original variety shows the same tendency as the normal marked variety.

  • PDF

New Malting Barley Variety "Sacheon# 6" (맥주맥 조숙 양질 다수성 신품종 "사천육호")

  • 박우형;정창해;최창휴;김병무
    • KOREAN JOURNAL OF CROP SCIENCE
    • /
    • v.25 no.4
    • /
    • pp.28-37
    • /
    • 1980
  • New malting barley variety "Sacheon #6" is an early maturing, medium height, lodging resistance, high tillering, good quality and high yielding. This variety was developed from the cross Harupin-Nizo x Hokudai #2 made in 1970 at Sacheon Malting Barley Experiment Station, Doo San Farms Co., This variety was determined to recommend to the all malting barley production areas in southern part of Korea from 1979.from 1979.

  • PDF

Genetic analysis on Some Quantitative Characters in Tobacco(Nicotiana tabacum L.) Breeding (연초(NIcotiana Tabacum L.) 육종을 위한 제형질의 통계유전학적 연구 III. 이면교잡에 의한 유전자 분포상태 및 우성정도추정)

  • Cho, Myung-Cho;Yu, Ik-Sang;Kim, Jin-Hyeong
    • Journal of the Korean Society of Tobacco Science
    • /
    • v.11 no.2
    • /
    • pp.157-179
    • /
    • 1989
  • This Study was conducted to estimate the degree of dominance and gene frequency of some sueful characters in tobacco. The eight parents and a set of 28 crosses of F'1s was F'2s were used as materials, and planted on oriental's and burley cultivated system as randomized block designs, respectively. The observed characters were six agronomic characters which were plant height, number of leaves per plant, leaf length, leaf width, days to flowering and yield, and the data obtained from the experiment were analyzed from methods by Hayman's and Jinks. The results obtained are summarized as follows: 1. In Vr-Wr graphical analysis, number of leaves per plant, leaf length, days to flowering and yield were found to be inherited in partial dominance, and plant height was over dominance to be similar to complete dominance, but leaf width was inherited with partial dominance close to complete dominance. 2. In the gene frequency, two varieties Xanthi-Basma and KA 102, for days to flowering and yield had larger number of dominant genes as those were situated near the point of origen. 3. Additive effects of genes(D) were greater than dominance effects of Genes(H) for six agronomic characters except plant height, and mean degree of dominance over all loci was lower than 1 for days to flowering yield, leaf length and number of leaves per plant.

  • PDF

Characteristics of a new cultivar Pleurotus ostreatus, Hwaseong #2 (신품종 느타리버섯 '화성2호'의 특성)

  • Lee, Jeong-Woo;Han, Yong-Sik;Han, Chul-Hee;Jeong, Chong-Chun
    • Journal of Mushroom
    • /
    • v.9 no.3
    • /
    • pp.96-100
    • /
    • 2011
  • MST247ns(Hwaseong #2) was developed by the method of Di-mon mating between monokaryotic strains derived from "Hwaseong #1" and dikaryotic strain "Suhan #1". The optimum temperature of mycelial growth was $25-30^{\circ}C$. The optimum temperature of primordia formation and fruiting body development were $8-15^{\circ}C$ and $9-14^{\circ}C$. Days of primordia formation were 4-5 days later Suhan #1. The stipes were longer than "Suhan #1". The surfaces of stipe were white and the tissues got harder and more elastic. Therefore, the management of growth environment under low temperatures was relatively easy and storability got much better.

Analysis of Genetic Relationship among Cymbidium germplasms Using RAPD and URP (RAPD와 URP를 이용한 심비디움 유전자원 유연관계 분석)

  • Park, Pue Hee;Kim, Mi Seon;Lee, Young Ran;Park, Pil Man;Lee, Dong Soo;Yae, Byeong Woo
    • FLOWER RESEARCH JOURNAL
    • /
    • v.18 no.3
    • /
    • pp.201-206
    • /
    • 2010
  • The genetic relationship among 48 Cymbidium cultivars was analyzed using randomly amplified polymorphic DNA (RAPD) with eighty 10 mers random primers (Operon Technologies) and twelve 20 mers random primers. Forty eight Cymbidium cultivars included 34 oriental Cymbidium, 7 hybrids, and 7 western Cymbidium. 407 (9.9 per primer) and 56 polymorphic bands (9.5 per primer) were generated by polymerase chain reaction with selected thirty 10 mers primers, and nine 20 mers primers, respectively. The polymorphic fragments ranged from 0.4 to 1.5 kb in size. The dendrogram was constructed by using the UPGMA clustering algorithm based on genetic similarity. Forty eight Cymbidium cultivars were classified into four major groups at similarity coefficient value of 0.638.

Breeding of Lilium × formolonga F1 hybrid 'Eorayeon 1ho' for Cut Flower (절화용 신나팔나리 F1품종 '어라연 1호' 육성)

  • Xuan, Yonghao;Kim, Jong Hwa
    • FLOWER RESEARCH JOURNAL
    • /
    • v.17 no.4
    • /
    • pp.324-327
    • /
    • 2009
  • The white flowered 'Eorayeon 1ho' is the first Kangwon National University cultivar of L. ${\times}$ formolonga, commonly known as seed propagating lily. Crosses were conducted from 1999 to 2003 among the selected selfing lines($S_3$) of L. longiflorum 'Lorina', L. ${\times}$ formolonga 'Augusta' and 'Raizan Herald'. A new cultivar was preliminarily selected as 'E-No.1' in 2003 for its stable upward flowering habit. The evaluation of characteristics was investigated from 2003 to 2004 in Chuncheon and Yungweol, Kangwon province. 'Eorayeon 1ho' blooms in early July at outside, with high resistance to Botrytis and having upward flowering habit. The plant height is 112.4 cm and the percentage of seed germination is 96.4%. This seed propagating cultivar shows good quality as a cut flower and low blasting rate.