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Analysis of Genetic Relationship among Cymbidium germplasms Using RAPD and URP  

Park, Pue Hee (Floriculture Research Division, National Institute of Horticultural & Herbal Science, Rural Development Administration)
Kim, Mi Seon (Floriculture Research Division, National Institute of Horticultural & Herbal Science, Rural Development Administration)
Lee, Young Ran (Floriculture Research Division, National Institute of Horticultural & Herbal Science, Rural Development Administration)
Park, Pil Man (Floriculture Research Division, National Institute of Horticultural & Herbal Science, Rural Development Administration)
Lee, Dong Soo (Floriculture Research Division, National Institute of Horticultural & Herbal Science, Rural Development Administration)
Yae, Byeong Woo (Floriculture Research Division, National Institute of Horticultural & Herbal Science, Rural Development Administration)
Publication Information
FLOWER RESEARCH JOURNAL / v.18, no.3, 2010 , pp. 201-206 More about this Journal
Abstract
The genetic relationship among 48 Cymbidium cultivars was analyzed using randomly amplified polymorphic DNA (RAPD) with eighty 10 mers random primers (Operon Technologies) and twelve 20 mers random primers. Forty eight Cymbidium cultivars included 34 oriental Cymbidium, 7 hybrids, and 7 western Cymbidium. 407 (9.9 per primer) and 56 polymorphic bands (9.5 per primer) were generated by polymerase chain reaction with selected thirty 10 mers primers, and nine 20 mers primers, respectively. The polymorphic fragments ranged from 0.4 to 1.5 kb in size. The dendrogram was constructed by using the UPGMA clustering algorithm based on genetic similarity. Forty eight Cymbidium cultivars were classified into four major groups at similarity coefficient value of 0.638.
Keywords
breeding; dendrogram; diversity; similarity;
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