• 제목/요약/키워드: 공초점 현미경

검색결과 184건 처리시간 0.028초

형광 공초점 주사 현미경의 측정 강도 향상을 위한 반사 광학계의 제안 및 설계 (Proposal and design of reflecting optical system to improve detection intensity in fluorescence confocal scanning microscopy)

  • 강동균;서정우;권대갑
    • 한국정밀공학회:학술대회논문집
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    • 한국정밀공학회 2002년도 춘계학술대회 논문집
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    • pp.187-190
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    • 2002
  • Confocal microscopy is very popular technology in bio-medical inspection due to its ability to reject background signals and to measure very thin slide of thick specimens, which is called optical sectioning. But intensity of detected signal in fluorescence type confocal microscopy is so small that only 0.2% of emitted fluorescence light can be detected in the best case. In this paper, we proposed the reflecting optical system to improve the detection intensity and designed the optical system by optimal design method. At the end of the paper, we analyzed the characteristics of the proposed reflecting optical system.

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물체표면의 3차원 정밀형상측정을 위한 공초점 현미경에 관한 연구 (A Study on Confocal Microscope for A Precise 3-Dimensional Surface Measurement)

  • 송대호;안중근;강영준;채희창
    • 한국정밀공학회:학술대회논문집
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    • 한국정밀공학회 1997년도 추계학술대회 논문집
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    • pp.233-236
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    • 1997
  • In modem industry, the accuracy and the surface-finish requirements for machined parts have been becoming ever more stringent. Optical method in measurements is playing an important role in vibration measurement, crack and defect detection and surface topography with the advent of opto-mechatronics. In this study, the principle of the general confocal microscope is introduced for surface measurement, and the advanced confocal microscope that has better measuring speed than the traditional confocal microscope is developed. A study on improving the resolution of the advanced confocal microscope is followed. Finally, Software for data acquisition and analysis of various parameters in surface geometrical features has been developed.

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공초점현미경과 원자현미경을 이용한 가공된 시료 표면의 형상측정 (Analysis of a processed sample surface using SCM and AFM)

  • 배한성;김경호;문성욱;남기중;권남익;김종배
    • 한국정밀공학회지
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    • 제23권4호
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    • pp.52-59
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    • 2006
  • Surface qualities of a micro-processed sample with a pulse laser have been investigated by making use of scanning confocal microscope(SCM) and atomic force microscope(AFM). Samples are bump electrodes and ITO glass of LCD module used in a mobile phone and a wafer surface scribed by UV laser. A image of $140{\times}120{\mu}m^2$ is obtained within 1 second by SCM because scan speed of a x-axis and y-axis are 1kHz and 1Hz, respectively. AFM is able to correctly measure the hight and width of ITO, and scribing depth and width of a wafer with a resolution less than 300nm. However, the scan speed is slow and it is difficult to distinguish a surface composed of different kinds of materials. Results show that SCM is preferable to obtain a image of a sample composed of different kinds of material than AFM because the intensity of a reflected light from the surface is different for each material.

Monte Carlo 방법을 이용한 공초점 주사 현미경의 오차 분석과 정렬 공차 할당에 관한 연구 (Error Analysis and Alignment Tolerancing for Confocal Scanning Microscope using Monte Carlo Method)

  • 유홍기;강동균;이승우;권대갑
    • 한국정밀공학회지
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    • 제21권2호
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    • pp.92-99
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    • 2004
  • The errors can cause the serious loss of the performance of a precision machine system. In this paper, we proposed the method of allocating the alignment tolerances of the parts and applied this method to get the optimal tolerances of a Confocal Scanning Microscope. In general, tight tolerances are required to maintain the performance of a system, but a high cost of manufacturing and assembling is required to preserve the tight tolerances. The purpose of allocating the optimal tolerances is minimizing the cost while keeping the high performance of the system. In the optimal problem, we maximized the tolerances while maintaining the performance requirements. The Monte Carlo Method, a statistical simulation method, is used in tolerance analysis. Alignment tolerances of optical components of the confocal scanning microscope are optimized to minimize the cost and to maintain the observation performance of the microscope. We can also apply this method to the other precision machine system.

공초점 현미경을 이용한 물체표면 형상측정에 관한 연구 (A Study on the Con-focal Microscope for the Surface Measurements)

  • 강영준;송대호;유원재;백성훈
    • 한국정밀공학회지
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    • 제20권5호
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    • pp.73-81
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    • 2003
  • In case of hollow cylinder extrusion using porthole die, the effects of extrusion parameters-temperature, the speed of extrusion, the shape of the die and mandrel-on metal flow in porthole die extrusion of aluminum have been investigated. However, there have been few studies about condenser tube extruded by porthole die. Original metal flow of condenser tube by porthole die extrusion is similar to hollow cylinder extrusion but the estimation of metal flow for extrusion parameters is different. For example, variation of chamber length in hollow extrusion only affects the welding pressure, however, the welding chamber length in condenser tube extrusion influences to the welding pressure as well as the deflection of mandrel. This study was designed to evaluate metal flow, welding pressure, extrusion load, tendency of mandrel deflection according to angular variation in the bottom of chamber in porthole die. Estimation was carried out using finite element method in as non-steady state. Analytical results can provide useful information the optimal design of porthole die.

LIF 공초점 현미경을 이용한 Y-채널 마이크로믹서의 혼합 효율 평가 (Mixing Efficiency Evaluation in Y-channel Micromixer Using LIF Confocal Microscope)

  • 김경목;신용수;안유민;이도형
    • 대한기계학회논문집B
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    • 제31권2호
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    • pp.159-166
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    • 2007
  • Mixing between two or more reagents is one of important processes in biochemical microfluidics. In efficient micromixer design, it is essential to analyze flow pattern and evaluate mixing efficiency with good precision. In this work, mixing efficiency for Y-channel micromixer is measured by fluorescence intensity using LIF(Laser Induced Fluorescence) Confocal Microscope. The Y-channel micromixers are fabricated with polydimethylsiloxane(PDMS) and those are bonded to glass plate through Plasma bonding. Nile Blue A is injected into the micromixer as a fluorescence dye for measuring of fluorescence intensity by He/Ne laser. For visualization of the flow pattern, dynamic image capturing is carried out using CAM scope. For the comparison with computer simulation, modified SIMPLE algorithm for incompressible flow equation is solved for the same geometry as in the experiment. Throughout the experiments and computer simulation, accurate mixing efficiency evaluation process for a PDMS Y-channel micromixer is established.

공초점반사현미경을 이용한 소나무 유연벽공의 초미세구조 연구 (Investigation of Bordered Pit Ultrastructure in Tracheid of Korean Red Pine (Pinus densiflora) by Confocal Reflection Microscopy)

  • 권오경
    • Journal of the Korean Wood Science and Technology
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    • 제42권3호
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    • pp.346-355
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    • 2014
  • 소나무의 가도관과 유연벽공의 미세구조를 연구하는데 공초점반사현미경법을 이용하여 획득한 3차원 화상을 사용하였다. 가도관 유연벽공의 토러스, 마르고, 벽공연의 미세구조가 명확하게 관찰되었으며, 교분야벽공의 미세구조로 가도관과 방사유세포 사이의 연결구조 및 방사유세포 내의 역학적 지지구조도 관찰할 수 있었다. 가도관 세포벽의 3차원 화상에서는 S1, S2, S3층과 이 층들의 사이에 있는 이행층의 존재도 확인할 수 있었다. 또한 S3층과 S2층의 마이크로 피브릴 배향의 관찰이 가능하였고, 유연벽공 주변의 복잡한 마이크로피브릴 배향 특성도 직접적으로 확인할 수 있었다. 본 연구의 결과 공초점반사현미경법은 소나무 가도관의 세포벽, 유연벽공, 교분야벽공의 미세구조를 연구하는데 유용하게 이용될 수 있는 현미경 기법으로 여겨졌다.

회전원판식 공초점 현미경의 구성과 광학특성 (Fabrication and studies on the properties of a spinning-disk confocal microscope)

  • 신은성;남기봉
    • 한국광학회지
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    • 제8권4호
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    • pp.255-259
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    • 1997
  • 본 연구에서는, Nipkow 원판을 사용하는 공초점 현미겨을 구성하여 시료의 2차원 영상을 얻었으며, 이들을 간단한 알고리즘으로 연결하여 표면의 3차원 영상을 얻었다. 마이크로 필름으로 제작한 Nipkow 원판의 적절한 경로로 통과하는 빛의 투과율은 0.5% 정도였으며, 따라서 반사율이 높은 물체만을 관찰할 수 있었다. 광원으로는 파장 692.7 nm의 반도체 레이저를 사용하였다. 구성된 현미경으로 전산기용 기억소자 칩 표면에 식각된 회로의 깊이와 거칠기를 관찰할 수 있었따. 재생전용 compact disk(CD)도 표면의 보호막을 제거하였을 때 표면에 있는 pit들의 배열 구조가 관찰되었다.

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