Proposal and design of reflecting optical system to improve detection intensity in fluorescence confocal scanning microscopy

형광 공초점 주사 현미경의 측정 강도 향상을 위한 반사 광학계의 제안 및 설계

Confocal microscopy is very popular technology in bio-medical inspection due to its ability to reject background signals and to measure very thin slide of thick specimens, which is called optical sectioning. But intensity of detected signal in fluorescence type confocal microscopy is so small that only 0.2% of emitted fluorescence light can be detected in the best case. In this paper, we proposed the reflecting optical system to improve the detection intensity and designed the optical system by optimal design method. At the end of the paper, we analyzed the characteristics of the proposed reflecting optical system.