• Proceedings of the KSAR Conference
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• 2003.06a
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• pp.70-70
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• 2003

조류의 배자는 포유류의 배자처럼 모체로부터 영양분을 공급받는 것이 아니라 알속에 저장되어 있는 영양물질로 발육한다. 배자의 발육은 대부분 체외에서 진행된다. 난자는 배란된 후 수정되어 난관팽대부에서 1세포기 수정란이 된다. 그 후 난관협부로 이동하여 최초로 분열이 일어나 배자의 발육이 진행되고, 산란시에는 세포수가 약6만여 개에 달한다. 따라서 수정란에 유전조작기법을 사용하기 위해서는 모체의 난관속에서 일어나는 배발생과 난각속에서 일어나는 개체발생을 위한 체외배양법과 대리난각 배양법이 확립되어 있어야 한다. 그와 같은 기술은 닭 수정란의 배 발생 관찰 및 분석과 유용한 물질을 생산하기 위한 형질전환 가금 연구에 중요한 기술로 사용되고 있다. 따라서 본 연구는 1세포기 닭 수정란의 체외배양과 대리난각 배양에 있어서 수정란의 조건과 대리난각의 조건이 배자의 생존율과 부화율에 미치는 영향을 조사하여 체외배양과 대리난각 배양에 의한 병아리 생산 효율을 제고하기 위한 기초자료를 얻고자 실시하였다. 주요 조사 항목은 수정란의 채란위치, 배자의 발생단계, 수정란의 무게, 대리난각용 계란의 보존 기간, 대리난각의 두께, 대리난각 두께의 감소율, 대리난각의 크기 등을 조사하여 배자의 생존율과 부화율에 미치는 영향에 대하여 조사하였다. 본 실험의 결과는 초기 발생이 빠른 배자가 생존율과 부화율이 높았으며, 본 실험에 사용한 대리난각용 계란의 보관기간이 짧을수록 배자의 생존율과 부화율이 높은 것으로 조사되었다(p<0.05). 그러나 대리난각용 계란의 크기와 대리난각의 두께 차이는 배자의 생존율과 부화율에 큰 영향이 없는 것으로 조사되었다.하였을 때 분할율은 68.0%였으며, 이중 12.0%가 상실배 또는 배반포로 발달하였다. 뿐만 아니라 10% FBS가 첨가된 TCM-199 배양액에 난관상피세포와 공배양을 실시하였을 경우는 72.0%가 분할하였으며, 이중 16.7%가 상실배 또는 배반포로 발달하였다. 이상의 결과로 볼 때 활성화 처리는 ionomycin과 6-DMAP 용액처리가 적합하며, 단위발생란의 체외배양은 보다 적합한 배양조건의 확립이 필요한 것으로 생각된다.icalcium lactate 공동배양은 체외배양에 별다른 영향을 미치지 못하는 것으로 나타났다.생산하는 것을 확인할 수 있었다.4시간에 등급별 회수율이 각각 GI(27.4%), GII(14.7%), GIII(43.2%), GIV(14.7%)로 나타났으며, 46~50시간에는 GI(12.0%), GII(12.0%), GIII(28.0%), GIV(48.0%)로 나타났다. 이상의 결과로 볼 때 미성숙 난자의 회수는 hCG 투여 후 29~34시간이 적합한 것으로 생각된다. 가금의 생산에 있어서 매우 효율적이고 주목할 만한 방법으로 사료된다. 것으로 나타났다.적외선.열풍 복합건조방법이 높게 나타나 이것은 곡물 표면에 원적외선 방사에의한 복사열이 전달되어 열장해를 받았기 때문으로 판단되며, 금후 더 연구하여 적정 열풍온도 및 방사체 크기를 구명해야 할 것이다.으로 보여진다 따라서 옻나무 유래 F는 포유동물의 생식기능에 중요하게 작용하는 것으로 사료된다.된다.정량 분석한 결과이다. 시편의 조성은 33.6 at% U, 66.4 at% O의 결과를 얻었다. 산화물 핵연료의 표면 관찰 및 정량 분석 시험시 시편 표면을 전도

• The Journal of the Korea Contents Association
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• v.14 no.6
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• pp.417-427
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• 2014

This study was investigated on the basis of the role of housing cooperatives in sustainable regional business networks(SLEN) in regeneration of residential aged area where the urban poor live. For the analysis, input elements of human capital, social capital, financial capital and ecological capital were examine. For the process, the organization's structure, sustainable business and social networks, and for the result, the stability and community characteristics of the living and the payment possibility of housing construction cost were examined. Through this study, what kinds of issues can be from the housing coop's project in terms of sustainability, and what is the most important factor in order to overcome former mentioned issues were intended to find. For analysis literatures were reviewed and the in-depth interviews and survey were conducted with site visits. The result of this study finds that housing coop have community characteristics through active participation of residents during the regeneration process but the land, as financial and ecological capitals, and housing construction funds were poor. This was investigated that it makes groundless the sustainability of the project and the possibility of the payment. In order to overcome, land concession, inexpensive lental and active funning for housing construction to housing coop is required because housing coop is aiming to supply of social housing for low-income and vulnerable groups.

• Journal of the korean academy of Pediatric Dentistry
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• v.35 no.4
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• pp.597-606
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• 2008

Resorption of alveolar bone in periodontitis is due to excessive differentiation and activation of osteoclasts. Bacterial antigens causing periodontitis activates CD4 T cells, which leads to expressing RANK ligand (RANKL) on CD4 T cells. RANKL binds RANK on preosteoclasts or osteoclasts, and enhances the differentiation preosteoclasts into osteoclasts and the activation of mature osteoclasts. CD137, one of TNF receptor (TNFR) family, expressed on activated T cells binds with CD137 ligand (CD137L) on antigen presenting cells. Cross-linking of CD137 by CD137L acts as T cell co-stimulatory signals and, therefore, enhances the activation of T cell. In this study, I elucidated the biological responses of CD137L on (pre)osteoclasts and RANKL on T cells in the context of in vivo interaction between T cells and osteoclasts. RAW264.7, murine monocytic cells, constitutively express CD137L. Ligation of CD137L with anti-CD137L mAb inhibited RANKL-induced osteoclast formation in a dosedependent manner. Bone marrow cells are expressed CD137L by the treatment with M-CSF. Cross-linking of CD137L abolished M-CSF/ RANKL-evoked the formation of multi-nucleated osteoclasts. Both mouse CD4 and CD8 T cells are expressed RANKL following their activation. Ligation of RANKL with OPG, the decoy receptor for RANKL, inhibited both CD4 and CD8 T cell proliferation. These effects were attributed to RANKL-induced apoptosis. These data indicate that CD137L and RANKL on osteoclasts and T cells, respectively provide them with inhibitory signal.

• Journal of the Economic Geographical Society of Korea
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• v.15 no.4
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• pp.708-720
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• 2012

The main aim of paper is to analyse relationships of objects, methods and main agents for the sustainable growth of community business (CB). Since the 1990s, Korea central-local governments have carried out a variety of policies to revitalize the rural economy, but many policies did not work effectively. The main reason for this is that those policies were simply focused on construct the hardware-based infrastructure without considerations of community capabilities. Recently, to overcome these problems, various kinds of community business policies are carried out across the country. Therefore, to avoid previous problems, the concrete and thorough analysis on the current CB has to be required. To do this analysis, four case studies on Sungmisan Village (in Mapo-Gu, Seoul) were taken and analysed in this paper. Results for this are as follows; Firstly, for the sustained growth of CB, it has to be required that the needs and demands of communities and residents are reflected. In the case of Sungmisan CB, residents were relatively satisfied with their community products, even though tastes and prices of those products were not very attractive. The reason for this is that those products were created by needs of local people. In this process, a market within the village was created and the basis of the management was established. Secondly, in order to secure a stable profit's structure that is necessary at the early stage of business settlement, creating related networks with Sungmisan CB is necessary. The CB established a stable profit structure by using mutual commodities. In particular, they linked closely and mutually so that visitors can buy their commodities. Lastly, for the sustainable management of the CB a common target local people should be set up. Furthermore, a system for income distribution has to be needed for protecting and solving potential conflicts.

• Journal of The Korean Association For Science Education
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• v.34 no.1
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• pp.33-42
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• 2014

There are complex interactions between personal and contextual features in teaching in a science museum. In order to analyze two teachers' holding classes in a science museum, the CHAT (cultural historical activity theory) was used as an analysis tool in a case study. The analyses of the results revealed that both teachers had difficulties teaching in a science museum due to the contradictions among the factors of subject, rules, community, and division of labor in the activity system. Although both teachers had trouble with similar contradictions, there appeared to be different sets of internalization and externalization of such conflicts depending on the difference in the teacher's perceptions and experiences about teaching in a science museum, the passion and the motivation of the teachers. These suggested that efforts should be preceded to foster the activity system providing both emotional and social supports to teachers in order to activate teaching in a science museum. Taking a teacher training course on teaching in a science museum was also found to fail to function as mediating artifacts. The course should provide not only the experience of executing proper teaching strategies for teaching in a science museum and the feedback on their teaching, but also the guidance on how to lead to optimal expansion of the factors in the activity system.

• The Journal of Sustainable Design and Educational Environment Research
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• v.16 no.3
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• pp.9-17
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• 2017

This study investigates and analyzes the convenience facilities to strengthen or mitigate the Barrier Free awareness and Kindergarten Barrier Free for the kindergarten school staff and the public facility officials of the education office. In addition, to improve the Barrier Free, the improvement facilities of the kindergarten were investigated and analyzed. First, from the institutional viewpoint, it is necessary to amend the existing regulations of the Act for the Promotion of the Convenience Promotion of Disabled Persons, Elderly and Pregnant Women in Korea, and to change the facilities for duties and recommended installation of the kindergarten and the Barrier Free certification standards. Second, in order to apply the Barrier Free certification system to the kindergarten in terms of facility environment, it is necessary to activate the barrier kind of the kindergarten so as to receive the Barrier Free certification by lowering the score of the items difficult to improve due to the terrain or building structure. Third, the perception of Barrier Free is still not high in terms of social awareness. The purpose of this study is to investigate the method of creating a Barrier Free for a kindergarten in the education and living space of children who are the socially underprivileged. Therefore, based on this study, it is anticipated that it will be an opportunity to promote change of Barrier Free in Kindergarten if an attempt is made to improve the Barrier Free certification index suitable for public kindergarten.

• The Journal of the Korea Contents Association
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• v.11 no.9
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• pp.506-514
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• 2011

The purpose of this study is to look into whether there is a possibility to develop the image of the local community in conjunction with green tourism, thus to explore the concrete ways to vitalize local community with diversified green tourism programs. In the pursuit of relating the research of community image-building with comprehensive local characteristics, this study selected the case of Jung Bang I village near Daejeon and analyzed the case through both written materials and on-site investigation. As a result of analysis, first, it was found that community image-building contributed positive effects in terms of the commercialization of the local community and its revitalization. Second, it was observed that community image-building led to form a "green route" that played a role as a passage to link townspeople to the country. It was also verified that those country villages that succeeded in image-building provided the places where people could experience the country and arts, which showed the potential of their roles as educational opportunities. In conclusion, this study will be able to suggest the direction to where the green tourism and image-building projects of the small communities near towns should go, and also provide a substantial guideline to related people regarding how to operate and maintain the local community.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.15 no.3
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• pp.1770-1777
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• 2014

Coastal settlements have a diversity of tangible or intangible local assets and are environmentally sensitive areas. The value of tangible or intangible local assets is often overlooked and there is no enough consideration for the inhabitants who have a various and acute stake in the development process of coastal settlements. The development of coastal settlements without consideration for the local circumstance, resident's needs, and local characteristics makes it difficult to achieve sustainable regeneration. There are also alienation, conflicts, and the loss of a authentic sense of place because of physical oriented development without regard to the social, economic, and cultural aspects. This research defines the components and types of coastal settlements and draws the characteristics of them around the fishery ports in Busan in the aspect of sustainable regeneration. It will be the important methods for an integrated regeneration including not only spatial and physical regeneration but also social and economic regeneration which has the preservation of local culture with living conditions, local economy activation, and community building. It will also provides the bases of sustainable development, attractiveness, and competitiveness of coastal settlements.

• Information Systems Review
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• v.13 no.2
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• pp.1-16
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• 2011

Recently, many companies have started to manage and support CoPs formally at the organizational level because of strategic usability of CoP. These companies are also seeking ways to motivate CoP members to actively participate in their groups. Accordingly, this paper proposes one way of increasing CoP activities by rearranging CoP members. In practice, active CoP members often lead their groups. Therefore, rearranging members can, eventually, be one method to motivate more individuals to participate in CoP activities. This paper first suggests a new approach in order to improve knowledge sharing activities at the organizational level based on rearranging members of current CoPs. Second, a mathematical model is presented which maximizes total BLS (Balanced Level Score) of company A with several constraints. Then a real world problem is changed to a popular problem, VRP to solve this problem. Third, the solution program was developed to find a meaningful solution.

• Journal of Digital Convergence
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• v.18 no.3
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• pp.43-48
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• 2020

This study analyzed the level of digital informatization and the level of human relationship satisfaction in the disabled group. The digital informatization level of the disabled refers to the level of smart access, smart capability, and smart utilization. For the sampling of persons with disabilities, the survey was conducted for persons aged 7 to 69 who were registered under the Welfare for Persons with Disabilities Act. Regression analysis utilized SAS 9.3.s through vitalization of local communities, and information education for the disabled is also important. The analysis results are as follows. First, the higher the digital access level of the disabled, the higher the level of interpersonal satisfaction. Second, the higher the digital information capability level of the disabled, the higher the level of interpersonal satisfaction. Third, the higher the level of digital information utilization of the disabled, the higher the level of interpersonal satisfaction. As a result, the higher the digital information level of the disabled, the higher the satisfaction level in the interpersonal relationship of the disabled. In addition, it was found that careful attention and policy review are needed to resolve the digital information gap of the government for the disabled who are marginalized groups. Therefore, it is necessary to provide the expansion of universal services of the marginalized groups including the disabled. In addition, it is important to form a culture in which the community embraces the marginalized class through vitalization of local communities, and information education for the disabled is also important.