• Proceedings of the Plant Resources Society of Korea Conference
• /
• 1999.05a
• /
• pp.53-53
• /
• 1999

• Proceedings of the Plant Resources Society of Korea Conference
• /
• 1999.05a
• /
• pp.52-52
• /
• 1999

• Proceedings of the Korean Society of Postharvest Science and Technology of Agricultural Products Conference
• /
• 2002.08a
• /
• pp.163.2-164
• /
• 2002

• Journal of Korean Society of Forest Science
• /
• v.98 no.1
• /
• pp.106-114
• /
• 2009

This present study was conducted to investigate photosynthetic characteristics, chlorophyll fluorescence, chlorophyll contents of Allium victorialis var. platyphyllum, Ligularia fischeri, Ligularia stenocephala growing under four different light intensity regimes (full sun, and 64~73%, 35~42%, 9~16% of full sun). Three wild vegetables showed good photosynthetic capacity in July. Allium victorialis var. platyphyllum showed best photosynthetic capacity and apparent quantum yield in 35~42% of full sun. But Ligularia fischeri and Ligularia stenocephala showed best photosynthetic capacity and apparent quantum yield in full sun and decreased as the shading level increaese. As the shading level increased, the total chlorophyll contents increased with a significant difference in three wild vegetables.

• Journal of the Korean Society of Food Science and Nutrition
• /
• v.45 no.1
• /
• pp.61-67
• /
• 2016

An HPLC analysis method was developed for standard determinations of chlorogenic acid, 3,4-di-O-caffeoylquinic acid, 3,5-di-O-caffeoylquinic acid, and 4,5-di-O-caffeoylquinic acid as functional health materials in Ligularia fischeri extract. HPLC was performed on a $C_{18}$ Kromasil column ($4.6{\times}250mm$, $5{\mu}m$ column) with a gradient elution of 0.1% (v/v) trifluoroacetic acid and acetonitrile at a flow rate of 1.0 mL/min at $30^{\circ}C$. The analytes were detected at 330 nm. The HPLC method was validated in accordance with the International Conference on Harmonization guideline of analytical procedures with respect to specificity, precision, accuracy, and linearity. The limits of detection and quantitation for the four compounds were 3.0~14.6 and $9.2{\sim}44.4{\mu}g/mL$, respectively. Calibration curves showed good linearity ($r^2$ > 0.999), and the precision of analysis was satisfied (less than 0.9%). Recoveries of quantified compounds ranged from 98.96 to 101.81%. This result indicates that the established HPLC method is very useful for the determination of marker compounds in Ligularia fischeri leaf extracts.

• Korean Journal of Plant Resources
• /
• v.10 no.4
• /
• pp.401-410
• /
• 1997

This experiment was conducted to investigate the opimum media, media amount and planting density of nutri-culture for the high quality and yield of wild vegetable, Liguliaria fischeri Turez. The fried rice hull was recognized best media among perlite, sand and fried rice hull for nutri-culture of Ligularia fischeri Turcz. The yield on fried rice hull media at early growing stage was lower, but higher than in other media at late growing stage. Therefore, total yield in fried rice hull media was 15-29% higher than that of the existing perlite media. Growth and yield of Ligularia fischeri Turcz. were not significantly different between medium amounts, therefore considering economical efficiency and stability, the optimum medium amount was 105 $\iota/m^3$, Yield by planting density increased in the high density and 50 plants/$m^2$ was considered as proper planting density in nutri-culture of Liguliaria fishcheri Turcz.

• Korean Journal of Food Science and Technology
• /
• v.48 no.5
• /
• pp.508-514
• /
• 2016

Effects of the ethyl acetate fraction from gomchwi (Ligularia fischeri) extract against high $glucose/H_2O_2-induced$ oxidative stress and in vitro neurodegeneration were investigated to confirm the physiological property of the extract. The ethyl acetate fraction of gomchwi extract showed the highest total phenolic contents than the other solvent fractions. An anti-hyperglycemic activity of the ethyl acetate fraction was evaluated using the ${\alpha}-glucosidase$ inhibitory assay, and the half maximal inhibitory concentration ($IC_{50}$) value for ${\alpha}-glucosidase$ was found to be $727.64{\mu}g/mL$. In addition, the ethyl acetate fraction showed excellent 2,2'-azino-bis (3-ethylbenzthiazoline-6-sulfonic acid) diammonium salt radical scavenging activity, and inhibition of malondialdehyde production. The ethyl acetate fraction also decreased intracellular reactive oxygen species, whereas neuronal cell viability against high glucose/$H_2O_2$-induced cytotoxicity was found to be increased. Finally, 3,5-dicaffeoylquinic acid as a main phenolic compound in the ethyl acetate fraction was analyzed by high-performance liquid chromatography. These results suggest that gomchwi might be a good natural source of functional materials to prevent diabetic neurodegeneration.

• Journal of Bio-Environment Control
• /
• v.22 no.3
• /
• pp.262-269
• /
• 2013

This study was carried out in order to examine the germination characteristics of Ligularia fischeri seeds, and it was to develop the more efficient pre-treatment and production system of the seeds. It was performed by two ways - temperature control (10, 15, 20 and $25^{\circ}C$) and shading treatment (Full sunlight, 35%, 50%, 75% and 95% of full sunlight). Seed pre-treatment before the each experiment was carried out by temperature (with low temperature and wetting treatment (LTW) for 0, 15, 30, 45 and 60days) and shading treatment (with drying at room temperature (DRT), drying at low temperature (DLT) and water soaking (WS) for 2 days). Seeds of L. fischeri were, regardless of seed pre-treatment, germinated well at $10^{\circ}C$ and the more temperature went up, the more germination rate went down. As a result of surveying shading treatment, 75% shading with DRT was the highest germination rate (68.1%) and 95% shading with WS was the lowest germination rate (48.6%). It was showed over-growth under 95% shading treatment experiment and withered in the full sunlight. As a result of surveying the whole experiment, L. fischeri seeds pre-treated with LTW for 15 days germinated well at $10^{\circ}C$ and under 50~75% shading treatment.

• Research in Plant Disease
• /
• v.21 no.1
• /
• pp.36-39
• /
• 2015

In June 2012 and 2013, a destructive stem rot symptoms of Ligularia fischeri occurred sporadically in Hoengseong-gun and Pyeongchang-gun Gangwon-do, Korea. The typical symptom included water-soaking on the main stem, rotting, wilting and blighting, which eventually leads to death of the plant. White mycelial mats were spread over lesions and brown sclerotia were formed on stems and near soil surface. The sclerotia were white to brown, spherical or irregular, 1-3 mm in size on potato dextrose agar (PDA), The optimum temperature range of hyphal growth was $25-30^{\circ}C$ and the hyphal diameter was $4-10{\mu}m$. The typical clamp connections were observed in the hyphae of the fungus grown on PDA. The resulting sequence of 695 bp was deposited in GenBank. A BLAST search revealed that sequences of the this isolates showed >99% identity with those of Sclerotium rolfsii. On the basis of the morphological characteristics and phylogenetic analyses of molecular markers ITS rDNA, the fungi were identified as S. rolfsii. A pathogenicity test was carried out to fulfill Koch's postulates. To our knowledge, this is the first report of S. rolfsii on Ligularia fischeri in Korea.

• Horticultural Science & Technology
• /
• v.28 no.4
• /
• pp.638-647
• /
• 2010

The genetic relationships in 5 species of $Ligularia$ were investigated using RAPD (Randomly Amplified Polymorphic DNA) and ITS (Internal Transcribed Spacer) sequences analyses. In RAPD analysis, sixty three of 196 arbitrary primers showed polymorphism. The amplified fragments ranged from 0.2 to 1.6 kb in size. The dendrogram was constructed by the UPGMA clustering algorithm based on genetic similarity of RAPD markers. A total of 16 accessions were classified into 5 major groups corresponding each species at the similarity coefficient value of 0.77. In the ITS sequence analysis, the size of ITS 1 was varied from 248 to 256 bp, while ITS 2 was varied from 220 to 222 bp. The 5.8S coding region was 164 bp in lengths. Forty nine sites (10.2%) of the 478 nucleotides were variable, and the G+C content of ITS region ranged from 49.4 to 53.5%. In the ITS tree, five species of $Ligularia$ were monophyletic, and $L.$ $taquetii$ was the first branching within the clade. $Ligularia$ $intermedia$ formed a clade with $L.$ $fischeri$ var. $spiciformis$ (BS=79), and $L.$ $stenocephala$ and $L.$ $fischeri$ were also claded. Two data sets were congruent, except of the position of $L.$ $fischeri$ var. $spiciformis$.