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http://dx.doi.org/10.9721/KJFST.2016.48.5.508

Effect of gomchwi (Ligularia fischeri) extract against high glucose- and H2O2-induced oxidative stress in PC12 cells  

Park, Sang Hyun (Division of Applied Life Science (BK21 plus), Institute of Agriculture and Life Science, Gyeongsang National University)
Park, Seon Kyeong (Division of Applied Life Science (BK21 plus), Institute of Agriculture and Life Science, Gyeongsang National University)
Ha, Jeong Su (Division of Applied Life Science (BK21 plus), Institute of Agriculture and Life Science, Gyeongsang National University)
Lee, Du Sang (Division of Applied Life Science (BK21 plus), Institute of Agriculture and Life Science, Gyeongsang National University)
Kang, Jin Yong (Division of Applied Life Science (BK21 plus), Institute of Agriculture and Life Science, Gyeongsang National University)
Kim, Jong Min (Division of Applied Life Science (BK21 plus), Institute of Agriculture and Life Science, Gyeongsang National University)
Lee, Uk (Division of Special Purpose Tree, National Institute of Forest Science)
Heo, Ho Jin (Division of Applied Life Science (BK21 plus), Institute of Agriculture and Life Science, Gyeongsang National University)
Publication Information
Korean Journal of Food Science and Technology / v.48, no.5, 2016 , pp. 508-514 More about this Journal
Abstract
Effects of the ethyl acetate fraction from gomchwi (Ligularia fischeri) extract against high $glucose/H_2O_2-induced$ oxidative stress and in vitro neurodegeneration were investigated to confirm the physiological property of the extract. The ethyl acetate fraction of gomchwi extract showed the highest total phenolic contents than the other solvent fractions. An anti-hyperglycemic activity of the ethyl acetate fraction was evaluated using the ${\alpha}-glucosidase$ inhibitory assay, and the half maximal inhibitory concentration ($IC_{50}$) value for ${\alpha}-glucosidase$ was found to be $727.64{\mu}g/mL$. In addition, the ethyl acetate fraction showed excellent 2,2'-azino-bis (3-ethylbenzthiazoline-6-sulfonic acid) diammonium salt radical scavenging activity, and inhibition of malondialdehyde production. The ethyl acetate fraction also decreased intracellular reactive oxygen species, whereas neuronal cell viability against high glucose/$H_2O_2$-induced cytotoxicity was found to be increased. Finally, 3,5-dicaffeoylquinic acid as a main phenolic compound in the ethyl acetate fraction was analyzed by high-performance liquid chromatography. These results suggest that gomchwi might be a good natural source of functional materials to prevent diabetic neurodegeneration.
Keywords
gomchwi; high glucose; oxidative stress; Ligularia fischeri;
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1 Kerem Z, Bilkis I, Flaishman MA, Sivan L. Antioxidant activity and inhibition of $\alpha$-glucosidase by trans-resveratrol, piceid, and a novel trans-stilbene from the roots of israeli Rumex bucephalophorus L. J. Agr. Food Chem. 54: 1243-1247 (2006)   DOI
2 Shang YF, Kim SM, Song DG, Pan CH, Lee WJ, Um BH. Isolation and identification of antioxidant compounds from Ligularia fischeri. J. Food Sci. 75: C530-C535 (2010)   DOI
3 Rice-Evans CA, Miller NJ, Paganga G. Structure antioxidant activity relationship of flavonoids and phenolic acids. Free Radical Biol. Med. 20: 933-956 (1996)   DOI
4 Kim SS, Park RY, Jeon HJ, Kwon YS, Chun W. Neuroprotective effects of 3,5-dicaffeoylquinic acid on hydrogen peroxide-induced cell deathin SH-SY5Y cells. Phytother. Res. 54: 243-247 (2005)
5 Donath MY. Targeting inflammation in the treatment of type 2 diabetes: Time to start. Nat. Rev. Drug Discov. 13: 465-476 (2014)   DOI
6 Brownlee M. The pathobiology of diabetic complications a unifying mechanism. Diabetes 54: 1615-1625 (2005)   DOI
7 Krentz AJ, Bailey CJ. Oral antidiabetic agents: current role in type 2 diabetes mellitus. Drugs 65: 385-411 (2005)   DOI
8 Papa S, Skulachev VP. Reactive oxygen species, mitochondria, apoptosis and aging. Mol. Cell. Biochem. 174: 305-319 (1997)   DOI
9 Comporti M. Free radicals, oxidative stress, and antioxidants. J. Siena Acad. Sci. 2: 13-26 (2012)   DOI
10 Heo HJ, Kim DO, Choi SJ, Shin DH, Lee CY. Potent inhibitory effect of flavonoids in Scutellaria baicalensis on amyloid $\beta$ protein- induced neurotoxicity. J. Agr. Food Chem. 52: 4128-4132 (2004)   DOI
11 Bidchol AM, Wilfred A, Abhijna P, Harish R. Free radical scavenging activity of aqueous and ethanolic extract of Brassica oleracea L. var. italic. Food. Bioprocess Tech. 4: 1137-1143 (2011)   DOI
12 Kwak JH, Choi GN, Park JH, Kim JH, Jeong HR, Jeong CH, Heo HJ. Antioxidant and neuronal cell protective effect of purple sweet potato extract. J. Agric. Life Sci. 57-66 (2010)
13 Piao XL, Kim HY, Yokozawa T, Lee YA, Piao XS, Cho EJ. Protective effects of broccoli (Brassica oleracea) and its active components against radical-induced oxidative damage. J. Nutr. Sci. Vitaminol. 51: 142-147 (2005)   DOI
14 Cho SD, Kim GH. Food product development and quality characteristics of Ligularia fischeri for food resources. Korean J. Food Preserv. 12: 43-47 (2005).
15 Bae JH, Yu SO, Kim YM, Chon SU, Kim BW, Heo BG. Physiological activity of methanol extracts from Ligularia fischeri and their hyperplasia inhibition activity of cancer cell. J. Bio-Environ. Control 18: 67-73 (2009)
16 Kim, DO, Jeong SW, Lee CY. Antioxidant capacity of phenolic phytochemical from various cultivars of plums. Food Chem. 81: 321-326 (2003)   DOI
17 Apostolidis E, Kwon YI, Shetty K. Inhibitory potential of herb, fruit, and fungal-enriched cheese against key enzymes linked to type 2 diabetes and hypertension. Innov. Food Sci. Emerg. 8: 46- 54 (2007)   DOI
18 Chang ST, Wu JH, Wang SY, Kang PL, Yang NS, Shyur LF. Antioxidant activity of extracts from Acacia confusa bark and heartwood. J. Agr. Food Chem. 49: 3420-3424 (2001)   DOI
19 Heo HJ, Cho HY, Hong BS, Kim HK, Kim EK, Kim BG, Shin DH. Protective effect of 4',5-dihydroxy-3',6,7-trimethoxyflavone from Artemisia asiatica against $A{\beta}$-induced oxidative stress in PC12 cells. Amyloid 8: 194-201 (2001)   DOI
20 Liu MH, Yuan C, He J, Tan TP, Wu SJ, Fu HY, Liu J, Yu S, Chen YD, Le QF, Tian W, Hu HJ, Zhang Y, Lin XL. Resveratrol protects PC12 cells from high glucose-induced neurotoxicity via PI3K/Akt/FoxO3a pathway. Cell Mol. Neurobiol. 35: 513-522 (2015)   DOI
21 Ha GJ, Lee DS, Seung TW, Park CH, Park SK, Jin DE, Kim NK, Shin HY, Heo HJ. Anti-amnesic and neuroprotective effect of Artemisia argyi H. (Seomae mugwort) extracts. Korean J. Food Sci. Technol. 47: 380-387 (2015)   DOI
22 Kim HY, Woo KS, Hwang IG, Lee YR, Jeong HS. Effects of heat treatments on the antioxidant activities of fruits and vegetables. Korean J. Food Sci. Technol. 40: 177-170 (2008)
23 Kim KH, Kim NY, Kim SH, Han IA, Yook HS. Study on antioxidant effects of fractional extracts from Ligularia stenocephala leaves. J. Korean Soc. Food Sci. Nutr. 41: 1220-1225 (2012)   DOI
24 Wright E, ScismBacon JL, Glass LC. Oxidative stress in type 2 diabetes: The role of fasting and postprandial glycaemia. Int. J. Clin. Pract. 60: 308-314 (2006)   DOI
25 Re R, Pellegrini N, Proteggente A, Pannala A, Yang M, Rice- Evans C. Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radical Bio. Med. 26: 1231- 1237 (1999)   DOI
26 Lovell MA, Xie C, Markesbery WR. Acrolein is increased in alzheimer's disease brain and is toxic to primary hippocampal cultures. Neurobiol. Aging 22: 187-194 (2001)   DOI
27 Hsieh HL, Yang CM. Role of redox signaling in neuroinflammation and neurodegenerative diseases. Biomed. Res. Int. 2013: 484613 (2013)
28 Gao Z, Huang K, Xu H. Protective effects of flavonoids in the roots of Scutellaria baicalensis georgi against hydrogen peroxideinduced oxidative stress in HS-SY5Y cells. Pharmacol. Res. 43: 173-178 (2001)   DOI
29 Ha JS, Park SK, Park CH, Seung TW, Guo TJ, Kang JY, Lee DS, Kim JM, Heo HJ. Neuronal cell protective effect of new green extract against $H_2O_2$-induced oxidative stress and analysis of bioactive compounds. Korean J. Food Sci. Technol. 47: 673- 679 (2015)   DOI
30 Zhao B. Natural antioxidants protect neurons in alzheimer's disease and Parkinson's disease. Neurochem. Res. 34: 630-638 (2009)   DOI