• Proceedings of the KAIS Fall Conference
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• 2008.11a
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• pp.400-402
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• 2008

본 연구는 오염토양 내 구리를 효과적으로 고정화시키는 조건을 찾고자 고정화제의 주입농도를 변화시켜가며 실험을 진행하였으며, 실험결과 고정화제의 주입농도가 높을수록 구리제거효율도 높아지는 것으로 나타났으며 3mole의 인산염을 주입했을 때 98%이상의 높은 제거효율을 보였다. 또한 잔류인의 농도를 낮추고 고정화반응을 촉진시키기 위해 알칼리제를 투입하였을 때, 알칼리제의 농도가 높을수록 구리오염 농도와 잔류인의 농도가 더욱 낮아짐을 알 수 있었다.

• Proceedings of the KIPE Conference
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• 2019.11a
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• pp.100-101
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• 2019

본 논문은 유도전동기 고정자 자속 기준 벡터제어에서, 슬립 관계식과 순환 최소자승법을 이용하여 회전자 저항 및 고정자 과도 인덕턴스를 동시에 추정하는 알고리즘을 제안한다. 모의실험을 수행하여, 추정 회전자 저항과 고정자 과도 인덕턴스가 제안된 방법에 의해 각각 실제 값에 수렴함을 보인다.

• Proceedings of the Korea Multimedia Society Conference
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• 2002.11b
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• pp.114-117
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• 2002

본 논문에서는 미세한 차이를 식별할 수 있는 Chaos 이론을 숫자 패턴 인식 분야에 응용한다. 먼저, 숫자 영상의 특징 정보들을 시계열 데이터로 변환한 후, 제안된 수정된 에농 시스템으로부터 숫자 어트랙터를 재구성하고, 어트랙터의 특성 분석을 위해 프랙탈 차원 특징을 나타내는 정보 차원값을 이용하여 숫자를 인식하는 새로운 알고리즘을 제안함으로써, 특수한 용도로 숫자를 전문적으로 빠르고 정확하게 인식하는 고정도 숫자 인식 시스템을 구현하였다.

• KSBB Journal
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• v.21 no.5
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• pp.384-388
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• 2006

The production stability of high-yielding mutants of Streptomyces lincolnensis immobilized on celite beads was examined in repeated batch cultures. We also explored the feasibility of immobilization of vegetative mycelial cells on pre-wetted celite beads, which is practical method for cell immobilization. Repeated transfer of immobilized cells into fresh medium every 10 days increased productivity of immobilized cells and maximum concentration of lincomycin, 1007 $({\pm}256)$ mg/L, was obtained at the end of the ninth cycle. A 1.4-fold higher productivity was obtained in immobilized-cell culture than that obtained by suspended-cell culture. When pre-wetted beads were inoculated with vegetative mycelia and cultured a slightly higher amount of immobilized cells and lincomycin was obtained more than those obtained by culture of spores immobilized on dry beads. This result indicates that immobilization of mycelial cells on pre-wetted beads was readily available. This technique is simple and no additional facilities are required for cell immobilization.

• Applied Biological Chemistry
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• v.48 no.3
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• pp.189-200
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• 2005

Biological nitrogen fixation is an important process for academic and industrial aspects. This review will briefly compare industrial and biological nitrogen fixation and cover the characteristics of biological nitrogen fixation studied in Azotobacter vinelandii. Various organisms can carry out biological nitrogen fixation and recently the researches on the reaction mechanism were concentrated on the free-living microorganism, A. vinelandii. Nitrogen fixation, which transforms atmospheric $N_2$ into ammonia, is chemically a reduction reaction requiring electron donation. Nitrogenase, the biological nitrgen fixer, accepts electrons from biological electron donors, and transfers them to the active site, FeMo-cofactor, through $Fe_4S_4$ cluster in Fe protein and P-cluster in MoFe protein. The electron transport and the proton transport are very important processes in the nitrogenase catalysis to understand its reaction mechanism, and the interactions between FeMo-cofactor and nitrogen molecule are at the center of biological nitrogen fixation mechanism. Spectroscopic studies including protein X-ray crystallography, EPR and $M{\ddot{o}}ssbauer$, biochemical approaches including substrate and inhibitor interactions as well as site-directed mutation study, and chemical approach to synthesize the FeMo-cofactor model compounds were used for biological nitrogen fixation study. Recent research results from these area were presented, and finally, a new nitrogenase reaction mechanism will be proposed based on the various research results.

• KSBB Journal
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• v.18 no.5
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• pp.412-417
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• 2003

We first investigated basic characteristics of reticulated polyurethane (PU) foams as microbial carriers. In general, the specific surface area of PU foams increases with respect to decreasing pore sizes. However, the number of microbes adhered on the unit surface of reticulated PU foams decreases with respect to decreasing pore sizes. Thus, as a result of totally considering all effects such as apparent density, hydrolysis rate, and adhesion, we can know that PU foams with 45 PPI is the most appropriate microbial carrier. In this study, we can also investigate the effect of various physico-chemical surface treatments on the adhesion of microbes on the surface of PU foams. We used a chitosan treatment, a PEI (Polyethylene Imine) treatment, a xanthane treatment and a plasma treatment. As a result of comparing all surface treatments, the plasma surface treatment was the best.

• Journal of the Institute of Electronics Engineers of Korea SD
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• v.49 no.8
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• pp.73-81
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• 2012

In this paper, a novel phase locked loop has been proposed using an analog band-selection loop. When the PLL is out-lock, the PLL has a fasting locking characteristic with the analog band-selection loop. When the PLL is near in-lock, the bandwidth becomes narrow with the fine loop. A frequency voltage converter is introduced to improve a stability and a phase noise performance. The proposed PLL has been designed based on a 1.8V $0.18{\mu}m$ CMOS process and proved by HSPICE simulation.

• Proceedings of the Korean Society of Precision Engineering Conference
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• 2002.05a
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• pp.1-13
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• 2002

최근 자동차가 편리성과 안전성을 추구함에 따라서 자동차 부품도 과거의 기계식 동작 방법에서 전자 제어 장치가 부착된 전자식 동작 방법으로 발전되어 가고 있는 추세입니다. 또한 연비 향상을 위하여 부품의 소형화가 급속히 추진되고 있습니다. 이러한 전자화, 소형화를 실현시키기 위해 자동차 부품에서의 정밀성이 요구되어 지고 있고, 부품 제조 측면에서는 고속화, 정확성이 요구되고 있는 실정입니다. 당사의 경우 CBS (Conventional Brake System)를 생산하다가 전자 제어 장치가 부착된 ABS (Anti-lock Brake System)의 생산을 시작할 때, 부품의 정밀도를 좌우하는 각 치수의 공차 영역이 전체적으로 CBS 대비 약 1/5 수준밖에 되지 않고, 가공 속도는 약 2 배가 빨라야 하기 때문에 생산 준비 단계에서 많은 시행 착오를 겪어야만 했습니다. 여기서 당사가 ABS를 양산하면서 겪은 경험을 토대로 기술토록 하겠습니다. 가공 기술의 경우 고속, 정밀 가공을 실현시키기 위해서 크게 4 가지 측면을 고려해야만 하며, 그 4가지는 설비, Tool Holder, Tool, Chip 측면입니다. 먼저 설비가 고정도가 되기 위해 고강성을 필요로 하는 것은 물론 가동중에 필연적으로 발생하는 열변형에 대한 대책을 수립하지 않으면 안됩니다. 이러한 열변형은 설비 설계 단계에서 대책을 수립할 수 있는 것도 있지만 주위 온도 변화에 의한 열변형처럼 사용 중에 발생되는 것은 설비 사용자가 장시간에 걸친 Data 를 확보하여 대책을 수립하지 않으면 안됩니다. 또한 Tool Holder의 정도도 일반적으로 사용하는 것보다는 고정도의 것이 요구되어지므로 Tool Holder Maker 에 반드시 요구 정도를 명기하여 주문하여야 합니다. Tool도 고속화를 실현시키기 위해 고정도의 다이아몬드 공구가 필요하였고 이를 국내에서 개발하기 위해 많은 시행 착오를 겪어야만 했습니다. 또한 Chip 배출이 고정도의 필수 고려 항목으로서 당사에서는 경우에 따라 여러 가지의 방법을 사용하고 있습니다. 조립 기술의 경우 스테인레스 박판을 열변형을 최소로 하면서 용접을 하기 위해서 레이저 용접을 도입하였고, 부품을 압입하면서 정확하고 정밀한 치수 셋팅을 위하여 서버 모타를 이용한 압입 방법을 사용하였습니다.

• Microbiology and Biotechnology Letters
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• v.13 no.3
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• pp.251-255
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• 1985

Nitrogen-fixing bacteria were isolated from the rice rhizosphere of various paddy fields in our country. The screening of 235 isolates for nitrogen-fixing ability resulted in the isolation of Enterobacter agglomerans NFB264 and three Klebsiella pneumoniae NFB 3, NFB 320. Plasmids of various molecular weight from 1.7 to more than 84 Mal. were detected by agarose gel electrophoresis in three out of four isolates. But, these plasmids had not any nitrogen-fixing genes. Hybridization experiments using Klebsiella pneumoniae M5al nitrogen-fixing genes, nif Q-K and nif DH, as probes revealed the presence of homologous sequences in the chromosomal DNA of all isolates. However the restriction patterns of nif genes of the isolates by various restriction endonucleases were different to those of Klebsiella pneumoniae M5al.

• KSBB Journal
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• v.6 no.4
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• pp.403-406
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• 1991

Lactic acid production yield was examined for commercial use by immobilizing Rhizopus oryzae with radiation induced polymer. The supporting material was synthesized by the low temperature radiation polymerization method, in which the microorganism was entrapped physically by contacting together in culture broth shaking for 24 hours. Support polymers with 5∼10vol-% monomers were able to increase their catalytic activities, consuming 65g/1 of glucose, producing 56g/1 of lactic acid, thus yielding 88% of product in general. But in free culture, the microorganism consumed almost all carbon sources and produced lactic acid only 60% even after 96 hours. The yield of the experiment under discussion was significantly high compared with conventional immobilization procedure.