Production of L(+)-Lactic Acid by Rhizopus oryzae after Immobilization in Polymer Supports with Gamma-ray Induced Polymerization

방사선중합체로 고정화된 Rhizopus oryzae의 유산생성

Lactic acid production yield was examined for commercial use by immobilizing Rhizopus oryzae with radiation induced polymer. The supporting material was synthesized by the low temperature radiation polymerization method, in which the microorganism was entrapped physically by contacting together in culture broth shaking for 24 hours. Support polymers with 5∼10vol-% monomers were able to increase their catalytic activities, consuming 65g/1 of glucose, producing 56g/1 of lactic acid, thus yielding 88% of product in general. But in free culture, the microorganism consumed almost all carbon sources and produced lactic acid only 60% even after 96 hours. The yield of the experiment under discussion was significantly high compared with conventional immobilization procedure.

방사선 중합체로 고정화한 Rhizopus oryzae의 유산생성 방사선 공중합 고분자로 Rhizopus oryzae를 흡착 고정화하였다. 이 고정화 균체는 고정화하지 않은 생균체보다 반응시간도 짧았으며 제품수율도 아주 높았다. 즉 글루코스를 주 탄소원으로 한 경우 고정화 하지 않은 균체 배양은 반응후 96시간에 탄소원을 거의 소비하고 유산생성 수율이 약60%에 다다랐으나 고정화 균체는 반응후 48시간 안에 수율이 약 88%이상으로 되었다. 또 중합체 중 모노머의 비율이 5-10%의 것이 고정화에 좋은 결과를 보였다.