• Title/Summary/Keyword: 건조기술

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Methodology of Chromosome Preparation and Banding Analysis in Gallus domesticus (닭 염색체의 분리 분석 방법에 관한 연구)

  • 손시환;오봉국
    • Korean Journal of Poultry Science
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    • v.14 no.2
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    • pp.89-96
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    • 1987
  • The purpose of this paper to present morphological normal chick chromosomes and develope avian cytogenetic techniques including chromosome preparation and banding technique. The early chick embryos provide a consistent source of material with hish mitotic cells. Although chick embryo tissue gives excellent preparations, the 4-5 days embryo is somewhat incovenient materials, Most imp of ant for avian Chromosome analysis are the technical protocols to achieve adequate preservation, spreading, and staining of the full chromosome complement. To precise chromosome analysis, pro-metaphase states are required. Best results of banding will be obtained from air dried slides prepared from early chick embryos that have been aged at least 1 week. Good G-banding differentiation is achieved by adequate trypsin digestion fellowed by staining in Goe,sa dye. The results of C-banding is influenced by many factors including the conditions of Ba(OH)$_2$, HCl treatment, and state of rinsing. In addition to precisely interprets banding patterns, the densitometric analysis is recommended.

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Fabrication of BCP/Silica Scaffolds with Dual-Pore by Combining Fused Deposition Modeling and the Particle Leaching Method (압출 적층 조형법과 입자 추출법을 결합한 이중 공극 BCP/Silica 인공지지체의 제작)

  • Sa, Min-Woo;Kim, Jong Young
    • Transactions of the Korean Society of Mechanical Engineers A
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    • v.40 no.10
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    • pp.865-871
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    • 2016
  • In recent years, traditional scaffold fabrication techniques such as gas foaming, salt leaching, sponge replica, and freeze casting in tissue engineering have significantly limited sufficient mechanical property and cell interaction effect due to only random pores. Fused deposition modeling is the most apposite technology for fabricating the 3D scaffolds using the polymeric materials in tissue engineering application. In this study, 3D slurry mould was fabricated with a blended biphasic calcium phosphate (BCP)/Silica/Alginic acid sodium salt slurry in PCL mould and heated for two hours at $100^{\circ}C$ to harden the blended slurry. 3D dual-pore BCP/Silica scaffold, composed of macro pores interconnected with micro pores, was successfully fabricated by sintering at furnace of $1100^{\circ}C$. Surface morphology and 3D shape of dual-pore BCP/Silica scaffold from scanning electron microscopy were observed. Also, the mechanical properties of 3D BCP/Silica scaffold, according to blending ratio of alginic acid sodium salt, were evaluated through compression test.

A Study on the Development of Ship Building for the Wooden Canoe by Piling-up Laminated Wooden Plates Kit [1] - A Design for canoe hull and laminating wooden plates for piling-up kit - (적층식 평판 키트형 카누 건조 기술 개발에 관한 연구 [1] - 카누 선체의 설계 및 적층식 평판 키트 분할 기법 -)

  • Kim, Heui-Jung;Kim, Shung-Hyun;Jeong, Jae-Hyun
    • Journal of Advanced Marine Engineering and Technology
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    • v.33 no.5
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    • pp.762-769
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    • 2009
  • A canoe is one of the most popular boats in leisure on the water. Generally canoe has been built by good wooden strips. And canoe built by FRP is used for business recently. But by the users' demands for good quality and the restriction to environmental pollution the wooden canoes are required for personal and canoe building business. The modern wooden canoes were built by the strip construction method are used typically. However it is not suitable for the mass productivity requires effective resource operations and managements of men, materials, times, and price. On this paper the new construction method, called the piling-up laminated construction method, is studied to building a canoe using the piling-up with the laminated wooden plates gives more productivity than the others. First a canoe with various curved surfaces is designed from 3D design system. And the hull of canoe model is divided horizontally to generating the laminated plates that will be converting real wooden plates available from the market and will be routed by the manufacturing machine. After the simulating and analyzing of piling-up with the laminated plates, the canoe is building with less times, less men, less resources, and lower price than other method, avoid of the requirements of additional building tools. On the next paper the constructing of a real canoe using the manufactured wooden plates will be studied.

Culture Condition and Inoculum Volume of Liquid Spawn on the Bottled Cultivation of Agrocybe cylindracea (버들송이(Agrocybe cylindracea)의 액체종균 배양조건과 접종량)

  • Cheong, Jong-Chun;Hong, In-Pyo;Jang, Kab-Yeul;Park, Jeong-Sik
    • The Korean Journal of Mycology
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    • v.31 no.2
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    • pp.94-97
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    • 2003
  • In order to shorten cultivation periods of Agrocybe cylindracea, liquid spawn was used in place of sawdust spawn. Optimum culture condition of liquid spawn was PSB (potato extract sugar broth) medium, $25^{\circ}C$ temperature and pH 7.0. And, optimum inoculum volume was $10{\sim}15ml$. Two grams per liter of dry weight of the fungal mycelia were obtained with 10l of culture bottle with liquid spawn after 10 days. In 850 ml-bottle of sawdust media, cultivation period using liquid spawn (30 days) was five days shorter compared with the cultivation period in sawdust spawn (35 days). Also, complete growth of sawdust media was increased to 92% (liquid spawn) from 75% (sawdust spawn) and yield of fruiting bodies was increased to about 10%.

Evaluation of the Basic Properties of Materials for Application of Functional Plaster Mortar (기능성 미장 모르타르의 현장 적용을 위한 재료별 기초 물성에 관한 평가)

  • Cho, Do-Young;Kim, Gyu-Yong;Miyauchi, Hiroyuki
    • Journal of the Korea Institute of Building Construction
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    • v.12 no.2
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    • pp.152-160
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    • 2012
  • The development of building must be accompanied with construction technology and performance of materials. In particular, wet processes have a high level of dependence on manpower and a low level of diversification of materials used. This study aimed to determine the applicability of various materials for wet process, mechanized construction and eco-friendly building materials through a comparison with dry premixed mortar. As a result, it was found that resin plaster and gypsum plaster's strength is lower than that of dry cement mortar, but their mechanization application, construction simplification, smoothness and bond strength are higher than that of dry cement mortar. And estimate that is valid as workability, bonding strength, eco-friendly building material in occasion of gypsum plaster.

Test Run of the Pilot Plant for Recycling of the Waste Aluminum Dross (알루미늄 페드로스 재활용 파일롯트플랜트 시운전 결과)

  • Park, Hyung-Kyu;Lee, Hoo-In;Choi, Young-Yoon
    • Resources Recycling
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    • v.14 no.4 s.66
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    • pp.41-46
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    • 2005
  • Waste aluminum dross is a major waste in the aluminum scrap smelters and its major components are alumina and metallic aluminum. In this study, waste aluminum dross was leached with sodium hydroxide solution to extract the remained aluminum into the solution, and aluminum hydroxide was recovered from the leached solution. The dross residue generated at the leaching step was recycled into alumina base ceramic materials through a series of treatments such as washing, drying and roasting. Also, a pilot plant was constructed and tested to demonstrate the developed technology. Four tons of waste aluminum dross could be processed per day. From the test run of the pilot plant, it was confirmed that the developed technology could be applied to commercialization.

Cavitation Erosion Behavior in Seawater of Gray Cast Iron Treated by High Hardness Electroless Nickel Plating (고경도 무전해 니켈도금된 회주철의 해수 내 캐비테이션 침식 손상 거동)

  • Park, Il-Cho;Kim, Seong-Jong
    • Proceedings of the Korean Institute of Surface Engineering Conference
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    • 2017.05a
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    • pp.119.2-119.2
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    • 2017
  • 무전해 니켈도금은 전기 공급 없이 환원재의 화학반응에 의해 도금이 진행되며, 복잡한 형상의 제품에도 균일한 도금 층을 형성시킬 수 있어 널리 적용되는 기술이다. 특히, 전기 니켈도금 층에 비해 무전해 니켈도금 층의 내식성과 내마모성이 우수하여 산업현장에서 가장 많이 사용되고 있다. 그러나 해양환경에서 빠른 유속 변화에 의해 발생되는 캐비테이션-침식 방지를 위한 무전해 니켈도금의 적용은 전무한 실정이다. 따라서 본 연구에서는 회주철의 캐비테이션-침식 방지를 위해 최적의 무전해 니켈도금 조건을 규명하고, 그 캐비테이션 저항성을 평가하고자 하였다. 무전해 니켈코팅을 위한 모재는 gray cast iron (FC250)을 $19.5mm{\times}19.5mm{\times}5mm$의 크기로 제작하였다. 회주철의 인장강도는 $330N/mm^2$이며, 그 성분 조성(wt.%)은 3.23 C, 1.64 Si, 0.84 Mn, 0.016 P, 0.013 S 그리고 나머지는 Fe이다. 시험편은 SiC 페이퍼 grit #1200까지 연마하였으며, 시험편의 표면 거칠기(centre line average, Ra)는 $1.6-2.1{\mu}m$ 범위 내로 제작하였다. 연마된 시험편은 증류수(distilled water) 세척 후 hot air로 건조하였다. 무전해 도금 전 시험편은 탈지를 위해 아세톤 용액(room temperature, RT)에서 3분간 초음파 세척하고, $90^{\circ}C$의 알카리 수용액으로 5분간 세척하였다. 그리고 표면활성화를 위한 산세척(acid pickling)은 5% sulfuric acid 용액에서 30초 동안 실시하였다. 무전해 Ni-P(electroless nickel, EN) 도금 전과 모든 과정마다 증류수로 시험편을 철저하게 세척하였다. EN 도금을 위한 도금욕(the bath)은 기존 문헌 연구를 통해 조성성분, 도금조건 및 변수들(the parameters)의 적절한 범위를 결정하였다. 도금조로 500mL 비커를 사용하였으며, 모든 시험편은 2시간 동안 EN deposition을 실시하였다. 캐비테이션 실험 결과 EN 도금의 표면경도가 증가함에 따라 캐비테이션 저항성도 현저하게 향상되었다.

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Application of Gamma Ray Irradiation to the Microbiological Safety of Dried Seafood Products (건조 수산물의 미생물학적 안전성 확보를 위한 감마선 조사 기술의 이용)

  • Choi, Jong-Il;Kim, Hyun-Joo;Kim, Jae-Hun;Ahn, Dong-Hyun;Chun, Byung-Soo;Lee, Ju-Woon
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.43 no.2
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    • pp.169-173
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    • 2010
  • This study evaluated the effects of gamma ray irradiation on the safety of dried seafood products. Dried salted squid, Engraulis japonica, Hizikia fusiformis, Mytilus coruscus, and Porphyra tenera were gamma-irradiated at doses of 0, 1, 3, and 5 kGy. The total bacterial populations were then enumerated on total plate count agar, and bacteria isolated from the samples were identified by 16S rDNA sequencing. In addition, the isolated strains were inoculated in the products to determine $D_{10}$ values. The total bacterial populations in the dried seafood products ranged from 3.40 to 6.59 log CFU/g, and those of yeasts and molds ranged from 2.21 to 4.56 log CFU/g. The sequence analysis identified Staphylococcus sp. as the most common species in the dried seafood products, except for dried P. tenera. The $D_{10}$ values of the contaminating bacteria were lower than 0.7 kGy, except for Deinococcus sp., which had a value of 1.39 kGy. This study demonstrated that gamma irradiation could be used to improve the safety of dried seafood products.

Detection of Genetically Modified Maize Safety-approved in Korea Using PCR (PCR을 이용한 국내에서 안전성이 확인된 유전자재조합 옥수수의 분석 방법)

  • Heo, Mun-Seok;Kim, Jae-Hwan;Park, Sun-Hee;Woo, Geon-Jo;Kim, Hae-Yeong
    • Korean Journal of Food Science and Technology
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    • v.35 no.6
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    • pp.1033-1038
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    • 2003
  • Four lines (MON810, GA21, NK603, and TC1507)of genetically modified maize(GMM) were recently approved after a safety-assessment by the Korea Food and Drug Administration (KFDA). In this study, five pairs of specific oligonucleotide primers, based on the gene sequences inserted into maize and zein gene as internal standards, were designed and a method using PCR was developed for monitoring GMM and GMM derived foods circulating in the market. MON810 and GA21 were detected in raw materials of feed and food in the Korean market.

Estimation of Bearing Capacity of Subbase and Subgrade Using Pavement Dynamic Cone Penetrometer (포장동적관입시험기(PDCP)에 의한 보조기층 및 노상 지지력 측정)

  • Roo, Myung-Chan
    • International Journal of Highway Engineering
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    • v.5 no.1 s.15
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    • pp.35-45
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    • 2003
  • This paper presents a theoretical approach for estimation of CBR-value of subbase course and subgrade using a portable pavement dynamic cone penetrometer(PDCP). The PDCP used in this paper was based on a design from South Africa and extensive studies by Kleyn(1982) and more recently by Liveneh and Ishai(1987) and Chua(1988). To date, California Bearing Ratio[CBR] value was studied mainly for application of pavement structural design. This study was initiated to develop a method of obtaining the in situ CBR-values of subbase and subgrade for the structural evaluation of pavements in the swift and inexpensive manner. PDCP tests were implemented at 20 different kinds of soil samples in the lab and test results were analysed by a theoretical approach introduced. The procedure presented provides acceptable and promising results.

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