• Title/Summary/Keyword: 건조기술

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Isolation and characterization of a monodehydroascorbate reductase gene in poplar (Populus alba × P. glandulosa) (현사시나무 monodehydroascorbate reductase (MDHAR) 유전자의 분리 및 발현특성)

  • Yoon, Seo-Kyung;Park, Eung-Jun;Bae, Eun-Kyung;Choi, Young-Im;Kim, Joon-Hyeok;Lee, Hyoshin
    • Journal of Plant Biotechnology
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    • v.41 no.4
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    • pp.194-200
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    • 2014
  • Monodehydroascorbate reductase (MDHAR) is an important enzyme that plays a role in the detoxification of reactive oxygen species (ROS) by maintaining reduced pool of ascorbate through recycling the oxidized form of ascorbate. In this study, we isolated a PagMDHAR1 gene from Populus alba ${\times}$ P. glandulosa, and investigated its expression characteristics. The PagMDHAR1 cDNA encodes a putative 434 amino acids containing FAD- and NAD(P)H-binding domains. Southern blot analysis indicated that a single nuclear gene encodes this enzyme. Northern hybridization analysis revealed that PagMDHAR1 is highly expressed in both suspension cells and flower tissues, while its expression levels were enhanced by drought, salt, cold, wounding and ABA. Therefore, PagMDHAR1 might be expressed in response to abiotic stress through the ABA-mediated signaling pathway in this poplar species, suggesting that the PagMDHAR1 plays an important role in the defense mechanisms against oxidative stress.

Distribution of Habitats and Ecology of Weedy Melons (Cucumis melo var. agrestis Naud.) in Korea (우리나라 야생잡초 참외의 자생지 분포지역 및 생태)

  • Lee, Woo-Sung
    • Horticultural Science & Technology
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    • v.31 no.5
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    • pp.652-655
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    • 2013
  • Natural habitats of weedy melons were distributed on the islands along and on the west and south coasts of Korean peninsula including Boryeong, Seosan (Taean), Seocheon, Okgu, Buan, Gochang, Yeonggwang, Muan, Shinan, Haenam, Jindo, Wando, Goheung, Yeocheon, Hadong, Namhae, Goseong, Tongyeong, Geoje, and Jeju islands including Jeju city, Bukjeju-gun and Nam Jeju-gun. Weedy melons were found growing wildly in or around the cultivated lands in these regions. Natural habitats of weedy melons were in and around the cultivated lands. Weedy melon plants were found most often in soybean fields, followed by fields of mungbean, sweet potato, pepper, sesame, cotton, and peanuts. The plants were also found growing wild in foxtail millet fields, rice paddy levees along the streams, upland field edges, watermelon fields, corn fields, vegetable gardens near farmhouse, orange fields, compost piles, fallow fields, roadside and home gardens. They inhabited in sunny and a little dry spaces in relatively low-height crop plant fields in general. The time of fruit maturity was from early July to late October with the most frequency in September according to post survey answer. Fruits dropped off from the fruit stalk when matured. This phenomenon was thought beneficial for perpetuation in the wild. The fruits were being used commonly for food and toys for children. It was thought that weedy melons were perpetuating through the cycle of human and animal feeding of the fruits, human and animal droppings, often mixed in compost, and application of the compost to crop fields by human.

Biofilm Formation and Low pH Viability of Cronobacter spp. (Enterobacter sakazakii) Isolated from Powdered Infant Formula and Infant Foods in Korea (국내 분유 및 영.유아식품에서 분리된 Cronobacter spp. (Enterobacter sakazakii)의 Biofilm 생성 특성 및 내산성 비교)

  • Kim, Sun-Ae;Lee, Yu-Mi;Oh, Se-Wook;Gwak, Hyo-Sun;Hwang, In-Gyun;Kang, Dong-Hyun;Woo, Gun-Jo;Rhee, Min-Suk
    • Food Science of Animal Resources
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    • v.29 no.6
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    • pp.702-708
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    • 2009
  • We investigated biofilm formation in various media, growth in low pH, and the hemolytic activity of 14 strains of Cronobacter spp. (Enterobacter sakazakii) isolated from a variety of foods including powdered infant formula (n=75), infant cereal (n=100), honey (n=30), and other infant foods (n=100) in Korea. The Cronobacter spp. adhered and formed biofilms on polyethylene, and a greater extent of biofilm was observed in nutrient-rich media. No clear difference in biofilm-forming ability was noted among the media constituents and the pattern of biofilm formation was strain-dependent. Seven strains out of 14 strains (50%) grew at pH 4.1, indicating that the acid resistance of these Cronobacter spp. isolated in Korea was relatively low. Hemolytic activity was not observed in any of the strains. This study provides basic information for the physiological and biochemical characteristics of Cronobacter spp. isolated from a variety of infant foods in Korea.

A study for High Efficiency Dewatering of Sludge Contained Fine Particles (미세입자(微細粒子)를 함유(含有)한 슬러지의 고효율(高效率) 탈수(脫水) 연구)

  • Lee, Jung-Eun;Lee, Jae-Keun
    • Resources Recycling
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    • v.15 no.4 s.72
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    • pp.36-43
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    • 2006
  • There was some difficulty dewatering properties due to small porosity diameter of cake, when pigment sludge contained fine particle was formed by cake under the dewatering. It was difficult to dewater the sludge with fine particles with the conventional mechanical dewatering method. This study was to improve the dewatering rate as discharging the water from porosity of cake easily, supplying the low heat to the cake layer. Thermal dewatering equipment of piston type to keep up constant temperature on the cake was set up and relative experiment was conducted for sludge of 200 g with fine pigment particle. As test results. filtration of 176.8 g, cake weight of 19.4 g, cake thickness of 4.2 mm was measured, and it was analyzed that the water content of cake was 47 wt% and dewatering velocity, which moaned the residual d교 sloid amount per dewatering area, was $2.1DS\;m^{2}{\cdot}cycle$. This results showed that filtration increased, cake weight and thickness decreased and dewatering velocity increased against mechanical dewatering method. And water content of cake decreased about 30%, so the result which dewatering rate improved was drew generally. The reason is that the inner vapor pressure working at the cake porosity increased as applying the low heat to the cake layer, which lead to discharge the water from porosity easily. Therefore, this study was estimated by the useful technology for sludge reduction.

Utilization of Kiwifruit Branches as a Wreath Material (화환 재료로서 참다래나무 절지의 이용)

  • Lee, Kyung Suk;Im, Myung Hee;Park, Yong Seo;Lim, Dong Guen;Park, Yun Jum;Heo, Buk Gu
    • FLOWER RESEARCH JOURNAL
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    • v.16 no.3
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    • pp.179-185
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    • 2008
  • This study was conducted to determine the characteristics and utilization of pruned kiwifruit branches as wreath materials. Amount of pruned branches produced from one 11~15 year-old kiwi plant cv. 'Hayward' can be used for one wreath with 76cm in diameter. Making a wreath with 76cm in diameter with the pruned kiwifruit branches required 75 branches with 31~40cm length. And the cost to make a wreath with 76cm in diameter for the Keaki and the paper bush were cheeper than those of winged spindle tree and Korean dogwood. Floral designers evaluated that the glossy, the delicacy and the unique form of cut branches, and their various hue were lower than that of the commonly-used cut branches, however, the beauty of curved line, the characteristics after drying, and the utilization and prospect of pruned kiwi branches were higher by 'over good' than that of the commonly-used branches. Consequently, it was assumed that the utilization of pruned kiwifruit branches can be contributed to the improvement of side income for a farm household, and the diversity wreath materials.

A Study for Remediation of Railroad Ballast Gravel Using Dry Washing Method (건식세척기술을 이용한 철도 도상자갈 정화 연구)

  • Bae, Jiyong;Jeong, Taeyang;Kim, Jae Hun;Lee, Sang Tak;Joo, Hyung Soo;Oh, Seung-Taek;Cho, Youngmin;Park, Duckshin
    • Journal of the Korean Society for Railway
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    • v.20 no.3
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    • pp.365-373
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    • 2017
  • This study proposes a newly developed dry washing method for removing pollutants such as total petroleum hydrocarbon (TPH) and oxidized iron from the surface of ballast gravel. A batch-type dry washing method showed a good performance in a previous study. In this study, a continuous-type dry washing system, instead of a batch-type system, was prepared to improve the efficiency of the system. A drier and a separator were also applied to this system as pre-treatment process, and the performance of this system was evaluated. In this experiment, blasting media was blasted on the polluted gravels through 12 nozzles by a pressure of $5-6kg/cm^2$ for 20-30 mins to remove TPH and oxidized iron. It was found to be possible to remove 80-90% of TPH and oxidized iron by using this system. Several ways to improve the performance were suggested in this study.

Cultivation of Paecilomyces tenuipes using Mini-kit, small culture container (소규모 재배상을 이용한 생동충하초 재배)

  • Nam, Sung-Hee;Lee, Kwang Gill;Yeo, Joo Hong;Lee, Heui Sam;Hwang, Jae Sam;Choi, Young-Cheol;Park, Kwan-Ho
    • Journal of Sericultural and Entomological Science
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    • v.50 no.2
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    • pp.116-121
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    • 2012
  • Cordyceps and its allies fungi has been described as a secret medicine that gives eternal youth and a long life. Some species of Cordyceps are sources of biochemicals, such ascordycepin, with interesting biological and pharmacological properties. Hence, it has been studied to uncover its pharmacological effect. We attempted to study the formation of fruiting bodies and to develop means of mass production Korean isolate of Paecilomyces tenuipes has been inoculated into silkworms, where it reproduced using culture container, mini-kit successfully. Culture container, mini-kit is composed of a cylinder-shaped body and lid. The container is made of translucent polyethylene terephthalate. The size of the container is $82{\times}75mm$, reduced by 10 times as compared with the conventional culture kit. The mini kit has many advantages - high culture amount, ability of maintaining optimal humidity, parasite-free cultivation and high-end appearance. With the kit, the optimal cultivation condition is under $22^{\circ}C$, culture period of 53 days. And synnemata of P. tenuipes could be kept fresh for 14 days at the temperature of under $10^{\circ}C$. Therefore, the Min-kit can be used in both ways as a culture container and a packing kit for end-user customers.

A Study on the Retouching Materials for Oil Paintings - Using Restoration Colors and Acrylic Gouache - (유화 작품 색맞춤용 재료 특성 연구 - 복원용 물감과 아크릴과슈를 중심으로 -)

  • Choi, Hee Jin;Kang, Dai Ill
    • Journal of Conservation Science
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    • v.37 no.5
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    • pp.426-439
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    • 2021
  • In this study, we compare the properties and estimate the durability of five oil colors that are the most popularly used colors in the conservation of oil paintings. A set of these colors was obtained form four manufacturers each, and their properties were analyzed by conducting deterioration experiments. Subsequently, we observed the colors and performed X-ray fluorescence analysis. As a result of colour observation and XRF analysis, it was confirmed that there are the differences according to the pigment types, mixing rations and the manufacturers even for colors having the same product name. The deterioration test indicated differences in the appearance of the colors ; for instance, the color difference was above 12.0 in most of the samples, including restoration color and acrylic gouache. In the case of Lemon Yellow a lot of discoloration and cracking occurred, and difference in gloss was ob served in Viridian of manufacturer C. Most cracks were ob served in the restoration color obtained from manufacturer B and were assumed to be because of the resin used by the manufacturer. Nevertheless, additional research will have to be conducted by controlling variables in order to find out the cause. Through this study, we demonstrated that retouching materials for conservation of oil painting differed in their physical properties according to the color and manufacturer. Therefore a conservator should be mindful during the selection and use of materials for retouching oil paintings.

Comparison of the physicochemical components of Lentinula edodes cultivars cultivated in sawdust medium (톱밥배지에서 재배된 표고버섯 품종별 이화학적 성분 비교)

  • Choi, Ji Yeon;Kim, Min-Sun
    • Journal of Mushroom
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    • v.19 no.3
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    • pp.184-190
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    • 2021
  • With the development of sawdust medium cultivation technology, Lentinula edodes (shiitake mushroom) has become the most extensively produced and consumed mushroom globally. In this study, the approximate composition, nutritional components, and bioactive compounds of L. edodes were analyzed and compared for the five sawdust-cultivated shiitake mushrooms cultivars namely Sanjo701ho, Sanjo707ho, Sanjo715ho, Chamaram, and L808. The approximate range of the composition of freeze-dried shiitake mushrooms was 4.06-5.92 g/100 g of ash, 0.75-1.02 g/100 g of crude fat, and 21.24-29.15 g/100 g of crude protein. Sanjo701ho had the highest trehalose content (9.60±0.08 g/100 g), total polyphenol content (3.49±0.04 mg GAE/g), and total flavonoid content (1.33±0.03 mg QE/g) among the other shiitake mushroom cultivars. The total amino acid content was as follows: glutamic acid>aspartic acid>leucine. Glycine, alanine, valine, isoleucine, leucine, and phenylalanine contents were significantly decreased in the following sequence: Sanjo707ho, Sanjo715ho, Sanjo701ho, Chamaram, and L808. Crude protein, trehalose, and six types of amino acids were identified as classification indicators for the five cultivars of sawdust-cultivated shiitake mushrooms.

Effects of Pollen Viability and Pistil Receptivity on Seed Set for Artificial Pollination in Strawberry (딸기 인공 수분시 화분 활력 및 암술의 수정 능력이 결실률에 미치는 영향)

  • Kim, Dae-Young;Yoon, Moo- Kyung;Do, Kyung-ran;Kim, Tae il
    • Korean Journal of Breeding Science
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    • v.41 no.4
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    • pp.496-501
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    • 2009
  • In order to promote the efficiency of strawberry breeding programs, pollen viability of strawberry, 'Seolhyang' was investigated using the fluorochromatic reaction test and seed set under controlled environment. Pistil receptivity was also assessed by hand pollination. Four varieties including 'Maehyang' were used for the test of pistil receptivity with 'Seolhyang' as a pollen parent. Pollen viability remained high for several days under dry conditions as below 33% relative humidity while the greatest loss of viability occurred at 76% relative humidity. The viability was rapidly decreased at high humidity and almost all grains were unviable in 7 days after storage. Pollen viability does not appear to be drastically reduced if the relative humidity is low. Therefore, humidity is more important factor than temperature for the pollen viability in Fragaria${\times}$ananassa. The rate of seed set by hand pollination lasted higher than the average of 77.2% from 2 to 8 days after emasculation when the daily average temperature was around $15^{\circ}C$ in plastic house. It began to decline gradually from 10 days and had decreased dramatically after 12 days except several cultivars. Based on the daily mean accumulated temperature, it is recommended to have an artificial pollination between the range of $45{\sim}140^{\circ}C$ after the emasculation to increase the rate of seed set in strawberry.