Food Science of Animal Resources (한국축산식품학회지)

Korean Society for Food Science of Animal Resources (한국축산식품학회)
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Biofilm Formation and Low pH Viability of Cronobacter spp. (Enterobacter sakazakii) Isolated from Powdered Infant Formula and Infant Foods in Korea

국내 분유 및 영.유아식품에서 분리된 Cronobacter spp. (Enterobacter sakazakii)의 Biofilm 생성 특성 및 내산성 비교

  • Kim, Sun-Ae (Division of Food Bioscience and Technology, College of Life Sciences and Biotechnology, Korea University) ;
  • Lee, Yu-Mi (Technical Laboratory, Givaudan Korea) ;
  • Oh, Se-Wook (Food Safety Research Group, Division for Food Industry Platform Technology, Korea Food Research Institute) ;
  • Gwak, Hyo-Sun (Food Microbiology Division, Food Safety Evaluation Department, National Institute of Food and Drug Safety Evaluation) ;
  • Hwang, In-Gyun (Food Microbiology Division, Food Safety Evaluation Department, National Institute of Food and Drug Safety Evaluation) ;
  • Kang, Dong-Hyun (Department of Food Science and Human Nutrition, Washington State University) ;
  • Woo, Gun-Jo (Division of Food Bioscience and Technology, College of Life Sciences and Biotechnology, Korea University) ;
  • Rhee, Min-Suk (Division of Food Bioscience and Technology, College of Life Sciences and Biotechnology, Korea University)
  • 김선애 (고려대학교 생명과학대학 생명공학과) ;
  • 이유미 ;
  • 오세욱 (한국식품연구원 산업원천기술연구본부 안전성연구단) ;
  • 곽효선 (식품의약품 안전평가원 식품위해평가부 미생물과) ;
  • 황인균 (식품의약품 안전평가원 식품위해평가부 미생물과) ;
  • 강동현 (워싱턴 주립대학교) ;
  • 우건조 (고려대학교 생명과학대학 생명공학과) ;
  • 이민석 (고려대학교 생명과학대학 생명공학과)
  • Published : 2009.12.31
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Abstract

We investigated biofilm formation in various media, growth in low pH, and the hemolytic activity of 14 strains of Cronobacter spp. (Enterobacter sakazakii) isolated from a variety of foods including powdered infant formula (n=75), infant cereal (n=100), honey (n=30), and other infant foods (n=100) in Korea. The Cronobacter spp. adhered and formed biofilms on polyethylene, and a greater extent of biofilm was observed in nutrient-rich media. No clear difference in biofilm-forming ability was noted among the media constituents and the pattern of biofilm formation was strain-dependent. Seven strains out of 14 strains (50%) grew at pH 4.1, indicating that the acid resistance of these Cronobacter spp. isolated in Korea was relatively low. Hemolytic activity was not observed in any of the strains. This study provides basic information for the physiological and biochemical characteristics of Cronobacter spp. isolated from a variety of infant foods in Korea.

본 연구는 국내에서 시판되고 있는 분유 및 영 유아식품에서 분리한 Cronobacter spp.(Enterobacter sakazakii) 14개 균주의 biofilm 생성 능력, 산성 환경에서의 생장, 용혈성을 관찰하였다. 연구 결과, 14개의 분리균주는 polyethylene에서 biofilm을 생성하였으며 영양성분이 풍부할수록 biofilm을 강하게 생성하였다. 영양성분의 종류에 따른 biofilm 생성 능력 차이는 뚜렷한 경향을 나타내지 않았으며, Cronobacter spp.의 biofilm 생성 능력은 균주별 특성에 의한 것으로 판단된다. 다양한 pH 환경에서 생장 특성을 관찰한 결과, 14개 중 7개의 균주(50%)가 pH 4.1에서도 생장할 수 있어 국외 균주에 비해 내산성이 비교적 낮게 나타났다. 한편 분리된 모든 균주에서 용혈성은 관찰되지 않았다. 본 연구는 국내 영 유아용 식품에서 분리된 Cronobacter spp.의 생리 생화학적 특성을 제시하였으며 이는 향후 Cronobacter spp. 연구 및 관련 식품산업의 저감화 기술 개발에 기초 자료로 활용될 수 있을 것으로 판단된다.

Keywords

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