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http://dx.doi.org/10.5010/JPB.2014.41.4.194

Isolation and characterization of a monodehydroascorbate reductase gene in poplar (Populus alba × P. glandulosa)  

Yoon, Seo-Kyung (Division of Forest Biotechnology, Korea Forest Research Institute)
Park, Eung-Jun (Division of Forest Biotechnology, Korea Forest Research Institute)
Bae, Eun-Kyung (Division of Forest Tree Improvement, Korea Forest Research Institute)
Choi, Young-Im (Division of Forest Biotechnology, Korea Forest Research Institute)
Kim, Joon-Hyeok (Division of Forest Biotechnology, Korea Forest Research Institute)
Lee, Hyoshin (Division of Forest Tree Improvement, Korea Forest Research Institute)
Publication Information
Journal of Plant Biotechnology / v.41, no.4, 2014 , pp. 194-200 More about this Journal
Abstract
Monodehydroascorbate reductase (MDHAR) is an important enzyme that plays a role in the detoxification of reactive oxygen species (ROS) by maintaining reduced pool of ascorbate through recycling the oxidized form of ascorbate. In this study, we isolated a PagMDHAR1 gene from Populus alba ${\times}$ P. glandulosa, and investigated its expression characteristics. The PagMDHAR1 cDNA encodes a putative 434 amino acids containing FAD- and NAD(P)H-binding domains. Southern blot analysis indicated that a single nuclear gene encodes this enzyme. Northern hybridization analysis revealed that PagMDHAR1 is highly expressed in both suspension cells and flower tissues, while its expression levels were enhanced by drought, salt, cold, wounding and ABA. Therefore, PagMDHAR1 might be expressed in response to abiotic stress through the ABA-mediated signaling pathway in this poplar species, suggesting that the PagMDHAR1 plays an important role in the defense mechanisms against oxidative stress.
Keywords
ABA; PagMDHAR1; Populus alba ${\times}$ P. glandulosa; ROS; stress;
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