• KOREAN JOURNAL OF CROP SCIENCE
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• v.65 no.4
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• pp.365-376
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• 2020

Immature embryogenesis is a useful process in wheat tissue culture, including transgenic technology, because of its high regeneration efficiency compared to that in other tissues. However, it is a very labor-intensive and time-restrictive method, because the preparation of immature embryos is limited to the optimal time after flowering. In this experiment, 'Speed Breeding', a breeding technique that accelerates breeding generation advancement by extending the photoperiod, was applied to the wheat variety 'Bobwhite'. A controlled growth room was constructed by adjusting the photoperiod (22-hour light/2-hour dark) using LED lights at temperature of 22℃. After vernalization of the Bobwhite seeds at 4℃ for 4 weeks, the seedlings were grown in a controlled growth room and a greenhouse to compare the heading date. In both conditions, calli were induced from immature embryos on the 11^th day after flowering. After 4 weeks, the calli were transferred to a regeneration medium. Regeneration efficiencies under greenhouse conditions and Speed Breeding conditions were determined as 45.05% and 43.18%, respectively. Antioxidant enzyme activity and reference gene expression analysis were performed to confirm the presence of stress due to an extremely long-day photoperiod. As a result, the antioxidant enzyme activity was not distinguished from that of the greenhouse condition. The reference gene expression analysis revealed that the PsaA and CDC genes were highly expressed under the Speed Breeding condition. However, expression of PsbA was similar expression in both conditions. These results will provide useful information for the application of immature embryogenesis to the wheat transformation system.

• Economic and Environmental Geology
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• v.37 no.6 s.169
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• pp.585-601
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• 2004

A general study of domestic black sandstone and black shale quarries has not been made. For this reason it is difficult to know how the matter really stands. The objectives of this study are to achieve systematical and scientific study of the distribution, occurrences and rock quality of black sandstone and black shale resources exploited in existing quarries in the Boryeong area. The black sandstone bed survey was made from 54 mine claims on 4 sheets. In the area, 140 black sandstone and 22 black shale quarries were ascertained in 37 mine claims. The general development information data from that existing quarries were collected and synthesized. Among these black sandstone quarries for gravestones, monuments and black shale quarries for inkstone are in operation. Most of the black sandstone quarries were closed throughout the Gaewhari, Suburi, Seongjuri district in the investigated area even though these quarries had played a prominent part in the production of black sandstone. In view of commercial dimension stones, raw materials from black sandstone are classified as corestone and fresh rock body according to the characteristics of their occurrences and shape. Black sandstone beds are characteristically well-jointed and are particularly subdivided into cubic or quadrangular blocks in 3 joint sets. The colors of these black sandstones show medium dark $gray\~grayish$ black judging from the Rock Color Chart. The black sandstone beds which are intercalated in the Amisan, Jogeri, Baegunsa, Seongjuri Formations of the Daedong Supergroup are about $1\~10\;m$ in thickness.

• Horticultural Science & Technology
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• v.29 no.5
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• pp.407-412
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• 2011

The germination of the buds in 'Hongisul' grapes is poor and especially the high ratio of shoots where the flowers failed to emerge it was difficult to secure a sufficient amount of harvest. Thus, in this study, the necrosis and the periodical change in the buds were observed morphologically and also the cause of necrosis of the bud was investigated in order to understand what was causing the low germination and flowering. There was no change in the size of the bud after June and based upon external observation, there wasn't any retrogression or withering. However, based on a microscopic examination, the ratio of whole bud and main bud necrosis of the 'Hongisul' grapes continued to increase after August, and specially the ratio of the main bud necrosis continued to increase up to October. As for the size of the buds on the shoots, the buds located on the $1^{st}-3^{rd}$ nodes from the basal part were small whereas the buds located on the $4-10^{th}$ nodes were comparatively larger in its size. The ratio of necrosis of the bud was the highest at the $1^{st}$ and $2^{nd}$ bud, meaning that the buds located at the basal part of the shoot were defective compared to those located at the end of the shoot. It was also found that when the growth of the shoot is active and the shoot diameter is thick, it hindered the development of the buds. Therefore, it was judged that long pruning of 'Hongisul' grapes would help the emergence of the flowers as you could use the buds that have comparatively developed better.

• Journal of The Korean Society of Grassland and Forage Science
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• v.5 no.2
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• pp.106-110
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• 1985

This trial was carried out to find out the optimum ridge and seeding rate for the seed production of Festuca arundinacea S. (variety Alta). It was composed of three ridge level (12, 18 and 24cm) and four seeding rates (10, 15, 20 and 25kg/ha) in a split-plot design with 4 replications. The experiment lasted from September 1979 to October 1981. 1. The beginning of heading state was May 12 and the date of full flowering stage was June 2. The optimum stage for the seed harvesting of Festuca arundinacea was about 43 days after beginning of heading stage. 2. The number of panicles were 157 per square meter from the best treatment 18cm ridge with 15kg seed/ha. And the 1000 grain weight was 2.79g for the treatment 12cm ridge with 10kg seed/ha. 3. The average seed yield for two years was 681kg/ha and the largest 831kg/ha for the treatment 18cm ridge with 25kg seed/ha. 4. the average germination rate of the harvested seed was 90.7 percent, and it showed a little higher germination rate from the lower level of seeding rate. 5. The average total DM production with two cuttings of aftermath was about 6715kg/ha. According to the increasing seeding rate and narrowing the ridge, DM yield was higher.

• Journal of Plant Biotechnology
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• v.42 no.4
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• pp.312-325
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• 2015

In this review, we summarized the trends of genomics and transcriptomics research on peach, a model species of Rosaceae. Peach genome maps have been developed from various progeny groups with many next-generation sequencing (NGS) based single nucleotide polymorphism markers. Molecular markers of qualitative traits and quantitative trait loci (QTL) such as fruit characteristics, blooming date, and disease resistance have been analyzed. Among many characteristics, markers related to flesh softening and flesh adhesion are useful for marker assisted selection. Through comparative genomics, peach genome has been compared to the genome of Arabidopsis, Populus, Malus, and Fragaria species. Through transcriptomics and proteomics, fruit growth and development, and flavonoid synthesis, postharvest related transcriptomes and disease resistance related proteins have been reported. Recently, development of NGS based markers, construction of core collection of germplasm, and genotyping of various progenies have been preceded. In the near future, accurate QTL analysis and identification of useful genes are expected to establish a foundation for effective molecular breeding.

• Korean Journal of Soil Science and Fertilizer
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• v.17 no.4
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• pp.356-362
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• 1984

A field experiment was carried out to investigate the sulfur effects on yield, growth and nutritive qualities of soybean in the different fertilizer application methods. Ammonium sulfate and super-phosphate were applied to the soil with levels of 0, 2, 4, and 6Kg S/10a and potassium sulfate, ammonium sulfate and thiourea were applied by foliar application with 2KgS/10a. The results obtained were as follows; 1. Grain and dry matter yield of soybean were increased by the increase of sulfur application and sulfur application showed positive effect on yield components. 2. Sulfur application increased the sulfur content and decreased N/S ratio in grain of soybean. 3. Protein content was increased by raising sulfur application up to 4Kg S/10a. Sulfur application influenced on amino acid composition of soybean protein. Cysteine and methionine contents were increased by sulfur application. 4. Lipid content of soybean grain and inorganic ion absorption by soybean plant were not influenced significantly by sulfur application. 5. In application effects, there was no significant difference among sulfur fertilizers, but foliar application was more effective than soil application.

• Journal of Bio-Environment Control
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• v.20 no.1
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• pp.45-49
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• 2011

This research was carried out to investigate frost damage of mulberry tree (Morus alba) according to topographic characteristics in Buan province. The first bud-break, leafing, blooming and harvest date of mulberry tree in 2010 were two, four, fourteen and eight days later than those in 2009, respectively. These results were that daily mean temperature during March and April in 2010 were lower than those in 2009 by $2.3^{\circ}C$ and $2.4^{\circ}C$. Frost damage of orchards at flat-bottomed valley, flat near hill and lake, and plain were 50.0%,12.0%, and 4.2%, respectively. Also, frost damage of branch of below 15 mm in diameter was serious than that of branch over 16 mm, but orchard at flat-bottomed valley was high as the range of 46.2~54.0%. These results in 2010 were caused by occurrence of below zero temperatures in leafing stage. Since then, many shoots came out at accessary bud on proximal and the top part of the branches. Therefore, frost damage of mulberry tree in Buan province in 2010 was caused by occurrence of below zero temperatures on April and topographic characteristics of orchard.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.37 no.4
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• pp.339-346
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• 1992

Self-and reciprocal-graftings were made between soybean varieties(cv.Hwangkeumkong, Paldalkong, Bokwangkong and Jangkyongkong) to assertain the relative role of shoot and root in soybean growth and yield determination. The influence of grafting per se on plant height and leaf number on main stem were negligible as evidenced by the insignificant differences between self-and reciprocal-graftings. The effect of grafting on flowering was different between different set of varieties grafted reciprocally. Self grafting delayed flowering 2 days and reciprocal grafting delayed another 2 days, in general. The effects of grafting on dry weights of different plant parts except pod were also insignificant. Reciprocal grafting increased pod dry weight in Hwangkeumkong and Paldalkong regardless whether they were used as scion or stock. Reciporcal grafting also influenced dry weights of Bokwangkong and Jangkyongkong significantly, but the response was different from those of Hwangkeumkong or Paldalkong. Jangkyongkong, when used as stock, decreased total dry weight of Bokwangkong significantly through the reduction of pod and stem dry weights. Stem diameter was increased by grafting, however, grafting decreased number of branches in all varieties. The average yields of self-and reciprocal-grafting were decreased by 11% and 24%, respectively, comparing to that of non-grafting, while average yield of reciprocal grafting was 14% lower than that of self grafting. Stem weight of grafted soybeans was decreased by 29-32% comparing to that of non-grafted soybeans, but the difference between self and reciprocal graftings was negligible. The genetic characteristics of scion was found to be more important than those of stock in determination of yield and stem weight. It was also indicated that varieties and characteristics are different in response to grafting.

• Applied Biological Chemistry
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• v.36 no.6
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• pp.547-552
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• 1993

Soybean nuclear extracts and S-100 were prepared to examine the soybean embryo factors which bind to the upstream region of soybean ${\beta}-conglycinin$ ${\alpha}'$ subunit gene. SEF3(soybean embryo factor 3), which is presumed to be a trans-acting factor for the expression of the gene, was detected in gel mobility shift assay using the DNA probe containing two AACCCA hexanucleotides. DNA probe containing CATGCAT or AACACA was used to find any other soybean embryo factor interacting with the upstream region of ${\beta}-Conglycinin$ ${\alpha}'$ subunit gene. It was found that there was no common DNA binding protein detected both in nuclear extracts and S-100. The relative levels of SEF3 binding activity both in nuclear extracts and S-100 of maturing soybean seeds were determined. SEF3 activity of nuclear extracts was first detected around 20 days after pollination and significantly increased around 32 days after pollination.

• Journal of The Korean Society of Grassland and Forage Science
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• v.27 no.1
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• pp.1-8
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• 2007

This experiment was conducted to determine the effect of growth stage and variety on the yield and quality of whole crop rice (WCR) at National Livestock Research Institute, RDA from 2003 to 2005. Two types of rice ("Chucheong" for food and "Hamasari" for feed) were harvested at six different growth stages (heading, flowering, milk, dough, yellow ripening and fully ripening stage). Crude protein content decreased with progressed maturity at harvest. As growth stage delayed, TDN (total digestible nutrient) content of all rice varieties increased. The average TDN content of two type of rice was about 60% (59.3) which was calculated from ADF(acid detergent fiber) content. The contents of ADF and NDF(neutral detergent fiber) decreased with harvest maturity. The highest DM(dry matter) yield was at the ripening stage (p<0.05) and "Chucheong" gave higher yields than for "Hamasari". In this experiment, the DM yield of all varieties was low. The yield of TDN followed a similar trend to DM yield. According to this results that dough to yellow ripening stage of harvest maturity will be recommendable as proper harvest time for whole crop rice silage.