• Journal of Life Science
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• v.12 no.5
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• pp.610-621
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• 2002

Conserved genes are importantly used to understand the major function in survival and replication of living organism. This study was focused on identification of conserved genes in microbial species and measuring the degree of conservation. For this purpose, in silico analysis was performed to search conserved genes based on the conservation level within microbial species. The ortholog list of COGs (Clusters of Orthologous Groups of proteins) in NCBI was used and whole genomes of 43 microbial species were included in that list. The distance value, derived from CLUSTALW multiple alignment program, was used as a descriptor of the conservation level of orthologs. It was revealed that 43 microbial genomes hold 72 conserved orthologs in common. The majority(72.2%) of the conserved genes was related to "translation, ribosomal structure and biogenesis" functional category. A GTPase-translation elogation factor(COG0050) was the best conserved gene from the distance value analysis. The 72 conserved genes, found in this research, would be useful not only to study minimal function genes but also new drug target among pathogens and to make a model of the virtual cell.tual cell.

• Korean Journal of Microbiology
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• v.54 no.4
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• pp.445-447
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• 2018

Leuconostoc lactis CCK940, which was isolated from kimchi obtained from a Korean traditional market, produced an oligosaccharide with a degree of polymerization of more than 4. In this study, the draft genome sequence of L. lactis CCK940 was reported by using PacBio 20 kb platform. The genome of this strain was sequenced and the genome assembly revealed 2 contigs. The genome was 1,741,511 base pairs in size with a G + C content of 43.33%, containing 1,698 coding sequences, 12 rRNA genes, and 68 tRNA genes. L. lactis CCK940 contained genes encoding glycosyltransferase, sucrose phosphorylase, maltose phosphorylase, and ${\beta}$-galactosidase which could synthesize oligosaccharide.

• Journal of Life Science
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• v.18 no.4
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• pp.565-571
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• 2008

Due to rapid development of bioinformatics technologies, various biological data have been produced in silico. So now days complicated and large scale biodata are used to accomplish requirement of researcher. Developing visualization and annotation tool using them is still hot issues although those have been studied for a decade. However, diversity and various requirements of users make us hard to develop general purpose tool. In this paper, I propose a novel system, Genome Viewer and Annotation tool (GenoVA), to annotate and visualize among genomes using known information and multiple relation graph. There are several multiple alignment tools but they lose conserved area for complexity of its constrains. The GenoVA extracts all associated information between all pair genomes by extending pairwise alignment. High frequency conserved area and high BLAST score make a block node of relation graph. To represent multiple relation graph, the system connects among associated block nodes. Also the system shows the known information, COG, gene and hierarchical path of block node. In this case, the system can annotates missed area and unknown gene by navigating the special block node's clustering. I experimented ten bacteria genomes for extracting the feature to visualize and annotate among them. GenoVA also supports simple and rough computational annotation of new genome.

• Korean Journal of Microbiology
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• v.54 no.2
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• pp.161-163
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• 2018

The genome of Flavivirga eckloniae $ECD14^T$ isolated from a seaweed Ecklonia cava was sequenced. The genome comprises a single circular 5,665,358 bp chromosome with a G + C content of 33.9%, 4,647 total genes, 4,595 protein-coding genes, 44 pseudo genes, and 52 RNA genes. CRISPER genes and sequences were not found and there were some phage remnants and transposons. This strain contains alginate lyase and ${\beta}$-glucosidase genes responsible for the degradation of seaweed polysaccharides.

• Korean Journal of Microbiology
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• v.54 no.4
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• pp.477-479
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• 2018

A Gram-positive, rod-shaped, ivory colored, and motile, Lactobacillus koreensis 26-25 was isolated from Korean kimchi. Strain 26-25 showed the ability of conversion from major ginsenosides into minor ginsenosides for which whole genome was sequenced. The whole genome sequence of Lactobacillus koreensis 26-25 consisted of one circular chromosome comprised of 3,006,812 bp, with a DNA G + C content of 49.23%. The whole genome analysis of strain 26-25 showed many glycosides hydrolase genes, which may contribute to identify the genes responsible for transformation of major ginsenosides into minor ginsenosides for its high pharmacological effects.

• The Proceedings of the Korean Institute of Illuminating and Electrical Installation Engineers
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• v.15 no.2
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• pp.31-38
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• 2001

LCD는 비발광형 소자이므로 배면광이 필요하다. 현재의 LCD 배면광용 냉음극 형광램프는 전기-광학적 특성을 좋게 하기 위하여 수은방전을 사용한다. 그러나 수은의 이용은 외부 온도에 따라 특성이 변하는 결점과 환경 문제가 있다. 이 결점을 보완하기 위하여 원통방전형, 미세방전형 그리고 평면방전형 등 세가지 방식의 무수은 램프가 개발되어 왔다. 원통 방전형 무수은 램프는 수은 대신 Xe을 사용한다. Xe 방전이 수축되는 것을 막기 위하여 한쪽 전극은 외벽에 코일형태로 감아서 사용한다. 그리고 코일형태의 전극의 권선 간격을 조절하여 균일한 방전을 얻는다. 이 형태는 무수은 냉음극 형광램프의 두배의 광속을 얻을 수가 있다. 미세방전형 무수은 램프는 두 개의 절연체로 절연되 금속 전극사이의 방전공간에 수많은 미세방전을 일으켜 발광시킨다. 이 방식은 대향 방전구조와 면 방전구조의 두가지가 있다. 이 방식은 전극이 유전체로 둘러쌓여 있으므로 수명이 높다. 새로운 평면방전형 무수은 램프를 개발하였다. 이 램프는 두 개의 유리평판 사이에 방전공간을 만들고 한쪽 유리면의 양쪽 가장자리에 두 개의 전극을 설치하여 면방전을 유도한다. 양쪽 유리면에는 삼원색 형광체를 도포하고 Xe을 봉입하여 Xe의 진공자외선으로 형광체를 발광시킨다. 이 램프는 전극이 유전체로 덮혀있어 수명이 길다. 실험결과 기체압력 6.7[kPa], 구동전압 1,130[V]에서 최대휘도 9,200[$cd/m^2$], 광효율 20.4[lm/W]을 었었고, 기체 압력 2.7[kPa] 구동전압 1,120[V]에서 최대효율 34.1[lm/W], 휘도 1,080[$cd/m^2$]을 얻었다. 현재 무수은 램프는 수은 램프에 비해서 광학적 특성이 좋지 못하다. 무수은 램프에서 좋은 광학적 특성을 얻기 위해 가장 중요한 것은 수축이 없이 방전을 확산시키는 것이다. 이를 위해서 램프구조와 구동법을 최적화하는 것이 필요하다. 또한 기체압력을 높임으로서 Xe의 여기복사를 얻을 수 있었다.

• Korean journal of applied entomology
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• v.17 no.4 s.37
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• pp.193-199
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• 1978

Five parents and their 10 $F_1$ crosses of maize (Zea mays L.) were tested by means of diallel analysis for the inheritance of peroxidase activity following Puccinia sorghi rust infection. Peroxidase activity was measured at day zero and at 2 days and 8 days after inoculation. Peroxidase activity was increased significantly by P. sorghi infection in all 15 genotypes after 8 days, but not after 2 days. Highly significant differences in peroxidase activity were detected among the 15 genotypes. The highly genera] resistant inbred, CM105, and its hybrids showed exceptionally high peroxidase activity in both healthy and infected plants. However, another highly resistant inbred, Oh545, showed exceptionally low peroxidase activity. Significant general combining ability (GCA) and specific combining ability (SCA) means quares were detected for peroxidase activity independent of disease. GCA mean spuares, however, were consistently a major contiribution to the inheritance of peroxidase activity in the infected plants whereas SCA men square contributions were minor. Rust resistant maize plants controlling mongenic dominant $Rp_1^d$gene showed stronger peroxidase reroxidase responses than their susceptible counterparts in the gel electrophoresis and densiometric tracings. The increased peroxidase activity occurred in both major leaf peroxidases, $Px_3\;and\; Px_7$.

• Journal of Korean Society of Forest Science
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• v.95 no.4
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• pp.435-442
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• 2006

A total of 167 peculiar haplotypes confirmed from 28 cpSR variants that were observed in 19 populations of Japanese red pine in Korea through cpSSR marker analysis. Thirteen individuals that showed identical haplotype dispersed evenly in 10 populations, and the average number of effective haplotype within population was 13.37. Estimate of genetic diversity (He) was 0.987 on the basis of cpSSR haplotype variants that was equivalent to or higher than the estimates reported in other studies on some forest tree species. Estimation of genetic diversity (S.I.) on the basis of cpSSR variants composing each haplotype revealed the highest estimate of 1.109 for the population of Gangwon-Yeongwol and the lowest estimate of 0.411 for the population of Gyeongbuk Mungyeong with the average of 0.887. Most of observed cpSSR variants appeared to exist commonly in 19 populations (97.62%), and genetic differentiation of cpSSR variants among populations was turned out to be weak (${\Phi}_{ST}=0.024$). Relatively fast rate of mutation of cpSSR marker might be a major cause for such weak population differentiation. There was no identical haplotype shared between 39 population pairs of 173 pair-wise population pairs. Estimation of genetic distance among 19 populations on the basis of population pairs was also impossible, that might be resulted from restricted migration among 19 populations. Considering the observed distribution patterns of cpSSR variants in addition to the previous studies on I-SSR variants, informations on the present geographic location and genetic status of populations should be considered together for effective sustainable management of the genetic resources of Japanese red pine in Korea.

• Microbiology and Biotechnology Letters
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• v.49 no.4
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• pp.534-542
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• 2021

Single-molecular guide RNA (sgRNA) plays a role in recognizing the DNA target sequence in CRISPR technology for genome editing and gene expression control. In this study, we systematically compared the length of the target recognition sequence in sgRNAs required for genome editing using Cas9-NG (an engineered Cas9 recognizing 5'-NG as PAM sequence) and gene expression control using deactivated Cas9-NG (dCas9-NG) by targeting the gal promoter in E. coli. In the case of genome editing, the truncation of three nucleotides in the target recognition sequence (TRS) of sgRNA was allowed. In gene expression regulation, we observed that target recognition and binding were possible even if eleven nucleotides were deleted from twenty nucleotides of the TRS. When 4 or more nucleotides are truncated in the TRS of the sgRNA, it is thought that the sgRNA/Cas9-NG complex can specifically bind to the target DNA sequence, but lacks endonuclease activity to perform genome editing. Our study will be helpful in the development of artificial transcription factors and various CRISPR technologies in the field of synthetic biology.