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Distribution Pattern of cpSSR Variants in Korean Populations of Japanese Red Pine  

Hong, Yong-Pyo (Department of Forest Genetic Resources, Korea Forest Research Institute)
Kwon, Hae-Yun (Department of Forest Genetic Resources, Korea Forest Research Institute)
Kim, Yong-Yul (Department of Forest Genetic Resources, Korea Forest Research Institute)
Publication Information
Journal of Korean Society of Forest Science / v.95, no.4, 2006 , pp. 435-442 More about this Journal
Abstract
A total of 167 peculiar haplotypes confirmed from 28 cpSR variants that were observed in 19 populations of Japanese red pine in Korea through cpSSR marker analysis. Thirteen individuals that showed identical haplotype dispersed evenly in 10 populations, and the average number of effective haplotype within population was 13.37. Estimate of genetic diversity (He) was 0.987 on the basis of cpSSR haplotype variants that was equivalent to or higher than the estimates reported in other studies on some forest tree species. Estimation of genetic diversity (S.I.) on the basis of cpSSR variants composing each haplotype revealed the highest estimate of 1.109 for the population of Gangwon-Yeongwol and the lowest estimate of 0.411 for the population of Gyeongbuk Mungyeong with the average of 0.887. Most of observed cpSSR variants appeared to exist commonly in 19 populations (97.62%), and genetic differentiation of cpSSR variants among populations was turned out to be weak (${\Phi}_{ST}=0.024$). Relatively fast rate of mutation of cpSSR marker might be a major cause for such weak population differentiation. There was no identical haplotype shared between 39 population pairs of 173 pair-wise population pairs. Estimation of genetic distance among 19 populations on the basis of population pairs was also impossible, that might be resulted from restricted migration among 19 populations. Considering the observed distribution patterns of cpSSR variants in addition to the previous studies on I-SSR variants, informations on the present geographic location and genetic status of populations should be considered together for effective sustainable management of the genetic resources of Japanese red pine in Korea.
Keywords
cpSSR variants; Japanese red pine; genetic diversity; population differentiation; genetic resources;
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1 노은운, 이재순, 최영임, 한무석, 한상억, 이효신, 이용섭. 2005. 잣나무의 엽록체 유전체. 국립산림과학원 연구자료 251호. pp. 74-139
2 Birky, C.W.Jr., F. Paul, and M. Takeo. 1989. Organelle gene diversity under migration, mutation, and drift: Equilibrium expectations, attroach to equilibrium, effects of heteroplasmic cells, and comparison to nucler genes. Genetics 121: 613-627
3 Nei, M. 1987. Molecular evolutionary genetics. Columbia University Press, New York
4 Vendramin G.G., L. Lelli, P. Rossi, and M. Morgante. 1996. A set of primers for the amplification of 20 chloroplast microsatellites in Pinaceae. Mol. Ecol. 5: 111-114
5 Wakasugi T., J. Tsudzuki, S. Ito, M. Shibata, and M. Sugiura. 1994. A physical map and clone bank of the black pine (Pinus thunbergii) chloroplast genome. Plant Mol. BioI. Rep. 12: 227-241   DOI
6 Ribeiro, M.M., C. Plemion R. Petit, G.G. Vendramin, and A.E. Schmidts. 2001. Variation in chloroplast singlesequence repeats in Portuguese maritime pine (Pinus pinaster Ait.). Theoretical Applies Genetics 102: 97-103   DOI
7 Hong, Y.-P., H.-Y. Kwon, K.-S. Kim, K.-N. Hong, and Y.Y. Kim. 2004. Discordance between geographical distribution and genetic relationship among populations of Japanese red pine in Korea revealed by analysis of I-SSR markers. Silvae Genetica 53(3): 89-92
8 김영중, 송정호, 조경진, 김용울, 구영본. 2002. 準人工交 配에 의한 리기다X테다 소나무 잡종종자 大量生産과 雌花芽의 生長特性. 한국육종학회지 34(3): 228-235
9 Richardson, B.A., S.J. Brunsfeld, and N.B. Klopfenstein. 2002. DNA from birds-dispersed seeds and wind-disseminated pollen provides insights into postglacial colonization and population genetic structure of white bark pine (Pinus albicaulis). Molecular Ecology 11: 215-227   DOI   ScienceOn
10 Provan, J., N. Soranzo, N.J. Wilson, D.B. Goldstein, and W. Powell. 1999. A low mutation rate for chloroplast microsatellites. Genetics 153: 943-947
11 Provan, J., N. Soranzo, N.J. Wilson, J.W.McNicol, G.I. Forrest, J. Cottrell, and W. Powell. 1998. Gene pool variation in Caledonian and European Scots pine (Pinus sylvestris L.) revealed by chloroplast simple-sequence repeats. Proceedings of the Royal Society of London. Series B. 265: 1697-1705
12 Black, W. C, IV. 1996. RAPDDIST 1.0. Department of Microbiology, Colorado State University, Fort Collins, CO. USA
13 Excoffier, L., P. Smouse, and J. Quattro. 1992. Analysis of molecular variance inferred from metric distance among DNA haplotypes: application to human mitochondrial DNA restriction data. Genetics 131 : 479-491
14 Yeh, F.C., R.C. Yang and T. Boyle. 1999. POPGENE v 1.31. Microsoft window-based freeware for population genetic analysis. Dept. of Renewable Resources. Univ. of Chicago Press, Chicago, USA
15 Shannon, C.E. 1948. A mathematical theory of communication. Bell System Tech. J. 27: 379-423   DOI
16 Felsenstein, J. 1993. PHYLIP (Phylogeny Inference Package) version 3.5c. Distributed by the author. Department of Genetics. University of Washington. Seattle, WA. USA
17 Parducci, L., A.E. Schmidts, A. Madaghi, M. Anzidei, and Go. Vendramin. 2001. Genetic variation at chloroplast microsatellite (cpSSRs) in Abies nebrodensis (Lojac.) Mattei and three neighboring Abies species Theoretical Applies Genetics 102: 733-740
18 Schneider, S., D. Roessli and L. Excoffier. 2000. Arlequin V2.000. A software for population genetics data analysis. Dept. of Anthropology and Ecology, University of Geneva, Geneva, Switzerland
19 Slatkin, M. 1987. Gene flow and the Geographic structure of natural populations. Science 236: 787-792   DOI
20 Tautz, D. 1993. Notes on the definition and nomenclature of tandemly repetitive DNA sequences. In: Pena S.D.J., R. Chakraborty, J.T. Epplen, and A.J. Jeffreys (eds.). DNA Fingerprinting: State of the Science. Birkhauser Verlag, Basel, Switzerland, pp. 21-28
21 Hong, Y-P., H.-Y. Kwon, and I.-S. Kim. 2006. I-SSR markers revealed inconsistent phylogeographic patterns among populations of Japanese red pine in Korea. Silvae Genetica (인쇄중)
22 Anzidei, M., A. Madaghiele, C. Sperisen, B. Ziegenhagen, and Go. Vendramin. 1999. Chloroplast microsatellites for analysis of the geographic distribution of diversity in conifer species. In: Gillet, E.M. (ed.). Which DNA Marker for Which Purpose? Final Compendium of the Research Project Development, optimization and validation of molecular tools for assessment of biodiversity in forest trees in the European Union DGXII Biotechnology FW IV Research Programme Molecular Tools for Biodiversity. URL http://webdoc.sub.gwdg.de/ebook/y/1999/ whichmarker/ index.htm