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Combined Effects of Bacteriocin of Enterococcus faecalis MJ-213 and Organic Acid on Listeria monocytogenes Inactivation (Listeria monocytogenes에 대한 Enterococcus faecalis MJ-213이 생산한 박테리오신과 유기산 혼합 처리의 항균활성)

  • Lim, Sung-Mee
    • Korean Journal of Microbiology
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    • v.45 no.1
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    • pp.41-47
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    • 2009
  • In this study, the effect of combining oragnic acid and bacteriocin of E. faecalis MJ-213 isolated from Meju against L. monocytogenes KCTC 3569 growth in BHI broth and ground pork was investigated. In combination, the effects of 256 BU/ml bacteriocin and 1.5% acetic acid, citric acid or lactic acid were synergistic and effective than those compounds alone in controlling the viable cell counts of L. moncytogenes. The addition of increasing concentrations of the bacteriocin or organic acids led to a marked decrease in the number of L. monocytogenes. The combining treatment of the bacteriocin (256 BU/ml) and organic acid (1.5%) in ground pork inoculated with L. monocytogenes (5 log CFU/ml) resulted in 1 to 2 log CFU/ml reduction of cell counts during storage at $4^{\circ}C$ for 60 h. Also, the bacteriocin of E. faecalis MJ-213 was relatively stable at $100^{\circ}C$ for 20 min.

대면적 Transformer coupled Plasma Source에서 파워결합에 관한 실험적 연구

  • 김희준;손명근;황용석
    • Proceedings of the Korean Vacuum Society Conference
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    • 1998.02a
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    • pp.166-166
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    • 1998
  • 반도체 공정에서 기존보다 큰 30cm 웨이퍼훌 이용하기 위해서 기존의 ECR, Helicon, ICP, 등 공정용 고 밀도 플라즈마 원들의 대면적화에 대한 연구가 세계적으로 진행되고 있다 현 상황에서는 평판형 안테 나룰 이용한 TCP가 대면적용 폴라즈마 원의 가장 유력한 후보로 여겨지고 있다 TCP롤 대면적화 하는 데 있어서 중요한 문제점으로는 대면적에서의 큰 안테나 인되턴스로 인한 임피던스 정합과 대면적에서 의 유전울질의 기계적 강도이다. 앓은 유전물질올 사용힐 수 있도록 대면적 TCP 플라즈마 원올 실계 저l작하였고 이차원 가열이론올 이용한 TCPRP code 률 이용하여 안테나의 반경옳 결정하였디 안테나의 인덕턴스 값올 줄이기 위해서는 주m수는 13.56MHz 보다 낮은 4-5MHz 부근에서 작동하는 RF 파워룰 선택하였다 이 파워 서플라이는 보통 사용되는 50n 흩력 입묘$\mid$던스훌 갖는 형태가 Of니라 LC 공진현상 올 이용하여 부하에 파워률 전달하는 형태이다 .. TCP 장치에 사용할 수 있도록 파워 서플라이 흩력 단에 안테나와 직혈로 가변 콘덴서를 달아서 임11I던스 정합올 힐 수 있게 하였다 안테나에 직훌로 달Of줌으 로써 안테니의 인덕턴스훌 훌여주는 효과훌 얻올 수 있다 안테나에 흐르는 전류룰 측정하기 위해서 사 각형 루프로 전류 픽업 코일을 만들었고 진공상태에서 RF 파워률 인가하고 안테나의 전류와 전압을 측정하여 픽업 코일걸과훌 조정하였다. 발생기체로는 헬륨올 사용하였고 1-100mTorr 의 압력범위에서 실험을 하였다 플라즈마롤 빌샘시키고 파워를 증가 시킴에 따라 E-H mode transition 현상이 관찰되었고 그 때의 임계 전류 값을 측정하였다. 압력이 낮올수록 모드 변화가 일어나는 전류의 값이 작았다 임계 전류는 압력에 대해서 선형적인 특성을 보였다 이는 압력이 낮을수록 유도걸힘이 더 잘 된다는 것을 의미한다 1 1 mTorr에서는 H-mode에서 안테나의 전류가 파워훌 증가시킴에 따라 계속 증가하였으니, 압력이 올라 갈수록 조금씩 증가하는 정도가 줄어들고. 100mTorr에서는 포화된 값을 나타냈다 H-mode로 넘어간 후 에는 파워가 증가황에 따라 안테나의 임피던스 값이 모든 압력영역에서 줄어드는 경황을 보였고, 이는 플라즈마의 인덕턴스에 의해서 안테나의 인덕턴스 기 감소되기 때문이다, 파워가 증가할수록 안테U오} 플라즈마 루프사이의 상호걸합이 증가하는 걸로 해석힐 수 있다 안테나의 인되턴스 변화보다는 저항.성 분의 변화가 컸다 하지만 전체 임피던스로 볼 때 저항성분이 상대적으로 작기 때문에 인덕턴스의 감소 가 더 큰 영향을 미치는 걸로 볼 수 있다. 하지만 플라즈마로의 파워 전달에는 저항성분만이 영향올 미 치므로 저항성분의 큰 변화는 파워가 많이 전달될올 의미한다 피워전달 효율을 계산해 본 결과 수 r mTorr 부근이 80-90% 정도의 높은 효율올 보였고 5mTorr 일 때가 가장 좋았다.

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Survival, isolation and characterization of Bacillus cereus from Sunshik (선식에서 Bacillus cereus의 분리 및 특성 조사와 열에 대한 사멸률 연구)

  • Cho, Yong-Sun;Jung, Eun-Young;Lee, Myung-Ki;Yang, Cheul-Young;Shin, Dong-Bin
    • Journal of Food Hygiene and Safety
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    • v.23 no.4
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    • pp.343-347
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    • 2008
  • Recently, Sunshik has been issued because of easy-cook and well-being food. Sunshik basically was made of the heated cereals. Amount of spore-forming Bacillus cereus was detected and it has been caused some problem of food safety. B. cereus was isolate from 57 out of 161 Sunshik samples resulting in the isolation rate of 35.4%. Quantitative analysis of 57 samples showed that 21 samples were less than 100 CFU/g, 33 samples were between 100 and 1,000 CFU/g and distinctively even 3 (1.9%) samples had over 1,000 CFU/g. Typical morphology of B. cereus isolated from Sunshik was observed on MYP agar and then further characteristics was identified by using VITEK 2 (Biomeriux, France). 53 strains out of 57 strains isolated from Sunshik (about 93.0%) produced diarrheal enterotoxin in brain heart infusion broth which was detected by the Bacillus cereus enterotoxin reversed passive latex agglutination test kit (Oxoid England). The D-values of the B. cereus spores were $75^{\circ}C$ (37.1mim), $80^{\circ}C$ (22.5mim), $85^{\circ}C$ (4.9mim), and $90^{\circ}C$ (3.1mim) respectively. The Z-value was calculated $12.8^{\circ}C$ in Sunshik sample inoculated with B. cereus. Therefore, the management of B. cereus in Sunshik is required for the food-safety.

Changes in Physicochemical Characteristics of Immature Barley Kernels during Roasting (미숙보리곡립의 볶음중 이화학적 특성변화)

  • Lee, Young-Tack;Seog, Ho-Moon;Kim, Sung-Soo;Kim, Kyung-Tack;Hong, Hee-Do
    • Korean Journal of Food Science and Technology
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    • v.26 no.3
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    • pp.336-342
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    • 1994
  • Changes in physicochemical characteristics were investigated for immature barley kernels roasted at $160{\sim}220^{\circ}C$ for $1{\sim}12$ min. Only small differences in chemical constituents including starch, protein, fat, ash, total dietary fiber, and ${\beta}-glucan were observed between immature and mature barley kernels. The amounts of 75% ethanol-soluble sugars and amino acids present in immature barley kernels were considerably higher than those in mature kernels, and gradually decreased in the process of roasting. Of free sugars, sucrose, raffinose, glucodifructose($GF_{2}$) and maltose were reduced by roasting. Glucose and fructose, simple reducing sugars, decreased at the early stage of roasting, followed by a slight increase at the later stage. Starch and nitrogen contents decreased slowly, while TDF(total dietary fiber) had a tendency to increase slightly. Stacking volume of immature barley kernels increased markedly, especially at the higher temperatures. L value of immature barley decreased throughout roasting, and a, b values increased at the early stage of roasting but b value decreased with continued roasting. The degree of roasting was strongly affected by the roasting temperature. Darkness of immature barley kernel, depending on the degree of roasting, was highly associated with concentrations of brown pigments extracted from roasted immature barley kernels.

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Studies on Thermal Resistance of Selected Yeast Strain for Pasteurization of Solid Packed Peach (복숭아 Solid Pack 적정(適定) 살균조건(殺菌條件) 구명(究明)을 위(爲)한 선발(選拔) 효모(酵母)의 열저항성(熱抵抗性)에 관(關)한 연구(硏究))

  • Koo, Young-Jo;Lee, Dong-Sun;Shin, Dong-Hwa;Yu, Tae-Jong
    • Korean Journal of Food Science and Technology
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    • v.13 no.1
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    • pp.43-52
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    • 1981
  • A series of thermal destruction studies of the most heat resistant yeast strain No. 15 among 61 isolates were conducted in order to establish the optimum pasteurization condition of peach solid pack. The results obtained are summarized as follows: 1. A survival curve of the selected yeast strain No. 15 at $58^{\circ}C$ consisted of heat labile and heat stable fraction, showing broken curve. 2. The actively growing cell showed less recovery rate than 10 day rested cell after agitation-growing for 90 hr. For heating menstrua, peptone solution gave higher recovery rate than peach juice. For recovery medium, YM agar gave higher recovery rate than peach juice agar. The selected yeast was more resistant to heat at pH 4.0 than at pH 3.5 in both heating menstrua and recovery medium. 3. Z value of TDT curve of the selected yeast (heating : at pH 3.5 in peach juice. recovery: at pH 3.5 in peach juice agar) was $4.8^{\circ}C$. 4. The selected yeast No. 15 was identified as Torulopsis candida. 5. In the inoculated pack test of 4 oz can, it was concluded that the optimum P.U. 70/5 was 168 (center temp; $78.5^{\circ}C$, initial temp; $18^{\circ}C$, processing time; 18 min, initial yeast count; $1.0{\times}10^7$ per can).

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Comparison of Carcass and Meat Quality Characteristics between Korean Native Black Pigs and Commercial Crossbred Pigs (재래흑돼지와 개량종 돼지의 도체 및 육질 특성 비교)

  • Choi, Yeom-Soon;Park, Beom-Young;Lee, Jong-Moon;Lee, Sung-Ki
    • Food Science of Animal Resources
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    • v.25 no.3
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    • pp.322-327
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    • 2005
  • This study was intended to provide basic information which might be useful in increasing added values to the pork from Korean native black pigs (KNP), through comparative evaluation on carcass characteristics and meat qualities between KNP and four exotic breed groups including Duroc (DD), Landrace (LL), Yorkshire (YY), and LYD-cross (1/4LL 1/4YY, 1/2DD), and the results were summarized as follows. Live weight carcass weight, dressed meat weight, skin weight, fat weight and bone weight of KNP were significantly (p<0.05) lower than those of DD, LL, YY and LYD-cross. Carcass yield and dressed meat yield were also significantly (p<0.05) lower in KNP than other 4 groups, however, total fat, skin, bone, kidney and kidney fat in percentage were significantly (p<0.05) higher for KNP than others. Dressed meat yield was highest for YY, followed by LYD-cross and KNP. Weight of belly was 6.12, 9.43, and 9.52 kg for KNP, YY and LYD-cross, respectively. Percent belly of KNP was $11.11\%$ while other groups ranged from 11.5 to $12.2\%$ Shear force value was not different among the treatments. KNP had significantly (p<0.05) lower water holding capacity (WHC), and cooking loss, but significantly (p<0.05) higher in juiciness comparatively. KNP had significantly (p<0.05) higher $a^{*}$ value while DD had significantly (p<0.05) lower than other breeds.

Comparison of Quality Characteristics of Korean Native Black Pork and Modern Genotype Pork during Refrigerated Storage after Thawing (해동시킨 재래흑돼지육과 개량종 돼지육의 냉장저장중 품질비교)

  • Kang, Sun-Moon;Kang, Chang-Gie;Lee, Sung-Ki
    • Food Science of Animal Resources
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    • v.27 no.1
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    • pp.1-7
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    • 2007
  • This study was carried out to compare the quality characteristics of korean native black pork (KNP) and modern genotype pork (MGP) during refrigerated storage after thawing. M. longissimus from 5 Korean native black barrows of 65.3 kg and 5 modern genotype barrows ($Landrace{\times}Yorkshire{\times}Duroc$) of 114.3 kg were frozen at $-80^{\circ}C$ for 1 month and stored at $2{\pm}0.3^{\circ}C$ for 7 days. The carcass weight and dressing percentage was significantly lower in pork from Korean native black pigs than modern genotype pigs (p<0.05). The crude fat and sensory quality were significantly higher in KNP than in MGP (p<0.05), and moisture, myristic acid, gamma-linolenic acid, linolenic acid, and cis-5,8,11,14,17-eicosapentaenoic acid levels were significantly lower in KNP (p<0.05). The pH value was significantly higher in KNP than in MGP after 5 days of storage (p<0.05). Drip loss was significantly lower in KNP than in MGP during storage (p<0.05). The TBARS value was significantly higher in KNP than in MGP after 5 days of storage (p<0.05). Overall, KNP had lower moisture and unsaturated fatty acid levels relative to MGP and showed less oxidative stability during storage, however it had higher crude fat content, water-holding capacity, sensory quality and color stability than MGP.

Effect of Sawdust-Bedded Thickness in Floors of Hanwoo on Meat Quality of M. Longissimus after Slaughter (한우우사 바닥의 톱밥두께가 도축 후 등심의 육질에 미치는 영향)

  • Kang, Sun-Moon;Park, Yeon-Soo;Lee, Ik-Sun;Kim, Tae-Sil;Pan, Jo-No;Lee, Sung-Ki
    • Food Science of Animal Resources
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    • v.28 no.2
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    • pp.196-203
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    • 2008
  • This study was carried out to investigate the effect of sawdust-bedded thickness in floors (SBTF) of Hanwoo on meat quality of M. longissimus after slaughter. The 7 mon-aged bulls (22 heads) were housed in single sheds ($3{\times}8\;m^2$/animal) bedded with sawdust of 10, 15, and 20 cm thickness for 20 mon prior to slaughter. The M. longissimus from carcasses were then stored at $4{\pm}0.2^{\circ}C$ for 9 d. SBTF had no significant effect on carcass traits and pH, water-holding capacity, drip loss, cooking loss, Warner-Bratzler shear force, aroma pattern with electronic nose, and sensory attributes of beef. The 20 cm group had the highest crude fat and the lowest n6/n3 (p<0.05). At 9 d, the 10cm group showed the highest total reducing ability and the lowest TBARS content (p<0.05). During storage, the 10 and 20 cm groups had lower MetMb content, higher OxyMb content, and redder meat color than the 15 cm group (p<0.05).

Growth of Ti on Si(111)-)-$7{\times}7$ Surface and the Formation of Epitaxial C54 $TiSi_2$ on Si(111) Substrate (Si(111)-$7{\times}7$ 면에서 Ti 성장과 C54 $TiSi_2$/Si(111) 정합 성장에 관하여)

  • Kun Ho Kim;In Ho Kim;Jeoung Ju Lee;Dong Ju Seo;Chi Kyu Choi;Sung Rak Hong;Soo Jeong Yang;Hyung Ho Park;Joong Hwan Lee
    • Journal of the Korean Vacuum Society
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    • v.1 no.1
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    • pp.67-72
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    • 1992
  • The growth of Ti on Si(111)-$7{\times}7$ and the formation of epitaxial C54 $TiSi_2$ were investigated by using reflection high energy electron diffraction(RHEED) and high resolution transmission electron microscopy(HRTEM). Polycrystalline Ti layer is grown on the amorphous Ti-Si interlayer which is formed at the Ti/Si interface by Ti deposition on Si(111)-$7{\times}7$ at room temperature (RT). HRTEM lattice image and transmission electron diffraction(TED) showed that epitaxial C54 $TiSi_2$ grown on Si substrate with 160 ML of Ti on Si(111)-$7{\times}7$ surface at RT, followed by annealing at $750^{\circ}C$ for 10 min in UHV. Thin single crystal Si overlayer with [111] direction is grown on $TiSi_2$ surface when $TiSi_2$/Si(111) is annealed at ${\sim}900^{\circ}C$ in UHV, which was confirmed by Si(111)-$7{\times}7$ superstructure.

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Fabrication and Scintillation Characteristics of LiPO3 glass scintillators with the lanthanides activators (란탄계열 원소를 활성체로 첨가한 LiPO3 유리 섬광체의 제작과 섬광특성)

  • Whang, J.H.;Lee, J.M.;Jung, S.J.;Choi, S.H.;Sumarokov, S. Yu.
    • Journal of Sensor Science and Technology
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    • v.12 no.3
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    • pp.139-148
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    • 2003
  • $LiPO_3$ glass scintillators were fabricated, and lanthanides(except Pm) oxides or chlorides were used as an activator. For the fabrication of $LiPO_3$ glasses, optimum heating conditions were obtained, and the photoluminescence of the glasses was measured by the monochromator. For the best transparency of the glass samples, optimum heating temperature and time are $950^{\circ}C$ and 90 min, respectively. It was found that Pr, Nd, Gd, Ho, Er, Tm, Yb and Lu do not work as activator; emission spectrums of samples with them were equal to those of samples without activators. In the case of samples with Europium, the peaks of emission spectrum of $Eu^{2+}$ and $Eu^{3+}$ were 420 nm and 620 nm respectively. And samples with $Ce^{3+}$ were about 380 nm, and $Tb^{3+}$ were about 550 nm. Glass scintillators with $Be^{3+}$, $Eu^{2+}$, and $Ce^{3+}$ were found to be more applicable to neutron detection. The result of neutron detection by Ra-Be sources showed that $Ce^{3+}$ was found to be the best activator of $LiPO_3$.