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Microbial Forensics: Comparison of MLVA Results According to NGS Methods, and Forensic DNA Analysis Using MLVA (미생물법의학: 차세대염기서열분석 방법에 따른 MLVA 결과 비교 및 이를 활용한 DNA 감식)

  • Hyeongseok Yun;Seungho Lee;Seunghyun Lim;Daesang Lee;Sehun Gu;Jungeun Kim;Juhwan Jeong;Seongjoo Kim;Gyeunghaeng Hur;Donghyun Song
    • Journal of the Korea Institute of Military Science and Technology
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    • v.27 no.4
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    • pp.507-515
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    • 2024
  • Microbial forensics is a scientific discipline for analyzing evidence related to biological crimes by identifying the origin of microorganisms. Multiple locus variable number tandem repeat analysis(MLVA) is one of the microbiological analysis methods used to specify subtypes within a species based on the number of tandem repeat in the genome, and advances in next generation sequencing(NGS) technology have enabled in silico anlysis of full-length whole genome sequences. In this paper, we analyzed unknown samples provided by Robert Koch Institute(RKI) through The United Nations Secretary-General's Mechanism(UNSGM)'s external quality assessment exercise(EQAE) project, which we officially participated in 2023. We confirmed that the 3 unknown samples were B. anthracis through nucleic acid isolation and genetic sequence analysis studies. MLVA results on 32 loci of B. anthracis were analysed by using genome sequences obtained from NGS(NextSeq and MinION) and Sanger sequencing. The MLVA typing using short-reads based NGS platform(NextSeq) showed a high probability of causing assembly error when a size of the tandem repeats was grater than 200 bp, while long-reads based NGS platform(MinION) showed higher accuracy than NextSeq, although insertion and deletion was observed. We also showed hybrid assembly can correct most indel error caused by MinION. Based on the MLVA results, genetic identification was performed compared to the 2,975 published MLVA databases of B. anthracis, and MLVA results of 10 strains were identical with 3 unkonwn samples. As a result of whole genome alignment of the 10 strains and 3 unknown samples, all samples were identified as B. anthracis strain A4564 which is associated with injectional anthrax isolates in heroin users.

A Dynamic Study of Women's Labor Market Transitions: Career Interruptions and its Determinants (여성의 동태적 노동공급 - 취업연속성과 첫 노동시장 퇴출행태를 중심으로 -)

  • 김영옥
    • Korea journal of population studies
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    • v.25 no.2
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    • pp.5-40
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    • 2002
  • Using detailed data of women's work history, this study analyses the transition process between employment and non-employment over the life history in order to identity individual and structural determinants in the processes. Korean women comprise very heterogeneous groups in terms of work continuity: one group having a continuous work history and another having an interrupted work experience. While 4.0% of total women have stayed in the labor market since leaving school, 17.3% have not worked outside at all and remaining 87.9% have experienced into and out of the labor market at least once. On the average, the cumulated time of employment per woman is 8.2 years and the cumulated time of unemployment is 13.1 years. Thus Korean women work a total of only 38.5% of their whole lifetime after leaving school. We can conclude that the increase of the employment rate of married women in Korea since the 1970s has been due to the increase of the new entrants with short or little working careers into the labor market, not to the increase of women's work continuity on the whole. A women's educational achievement does not seem to be positively related to employment duration, contrary to the suggestion of the human capital theory, Rather, family variables, especially the existence of the child under 6 yens old, is a more significant determining factor for an individual's exit from employment. And there is little difference among different age cohorts which implies little improvement in the employment continuity of younger women. This study also documents the importance of structural variables, such as the type of occupation, as significant determining factors for the hazard rate. Specially women with professional jobs tend to stay longer in the labor market. Therefore, women's entry into more professional occupations is expected to contribute to the continuity of employment. Our results also show that duration-dependence is not spurious. When unobserved heterogeneity is controlled, the negative relation between the rate from employment and the duration of employment does not disappear.

Design and Fabrication of on Oscillator with Low Phase Noise Characteristic using a Phase Locked Loop (위상고정루프를 이용한 낮은 위상 잡음 특성을 갖는 발진기 설계 및 제작)

  • Park, Chang-Hyun;Kim, Jang-Gu;Choi, Byung-Ha
    • Journal of Navigation and Port Research
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    • v.30 no.10 s.116
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    • pp.847-853
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    • 2006
  • In this paper, we designed VCO(voltage controlled oscillator} that is composed of a dielectric resonator and a varactor diode, and the PLDRO(phase locked dielectric resonator oscillator) that is combined with the sampling phase detector and loop filter. The results at 12.05 GHz show the output power is 13.54 dBm frequency tuning range approximately +/- 7.5 MHz, and power variation over the tuning range less than 0.2 dB, respectively. The phase noise which effects on bits error rate in digital communication is obtained with -114.5 dBc/Hz at 100 kHz offset from carrier, and The second harmonic suppression is less than -41.49 dBc. These measured results are found to be more improved than those of VCO without adopting PLL, and the phase noise and power variation performance characteristics show the better performances than those of conventional PLL.

Integral Imaging System Enabling Enhanced Depth of Field Incorporating a Birefringent Liquid Crystal Lens Array (복굴절 특성을 갖는 액정 렌즈어레이를 이용한 깊이감이 향상된 집적영상 시스템)

  • Park, Chan-Kyu;Hwang, Yong-Seok;Lee, Sang-Shin
    • Korean Journal of Optics and Photonics
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    • v.19 no.6
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    • pp.394-399
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    • 2008
  • An integral imaging system enabling enhanced depth of field by incorporating a pair of liquid-crystal (LC) lens arrays was proposed and demonstrated. The lens arrays exhibit two different refractive indexes depending on the light polarization. The proposed LC lens array I and II were implemented by depositing a ZLI-4119 LC and an E-7 LC, respectively, on top of a lens-array substrate in glass. When the two LC lens arrays were aligned appropriately, a birefringence was obtained for a specific light polarization in such a way that the incoming light sees different refractive indexes for them. As a result, the focal length associated with the imaging system utilizing the LC lens arrays was adaptively varied, thereby enhancing the depth of field for the image reconstruction. We have theoretically analyzed the proposed integral imaging system with the $LightTools^{(R)}$ to confirm that the focal length could be adjusted with the help of the birefringent lens array. Finally the proposed imaging system successfully reconstructed the objects. The birefringent lens array employing the ZLI-4119 LC produced a real image with the focal length of 680 mm, while the other using the E-7 LC yielded a virtual image with the focal length of -29 mm.

High-Order Temporal Moving Average Filter Using Actively-Weighted Charge Sampling (능동-가중치 전하 샘플링을 이용한 고차 시간상 이동평균 필터)

  • Shin, Soo-Hwan;Cho, Yong-Ho;Jo, Sung-Hun;Yoo, Hyung-Joun
    • Journal of the Institute of Electronics Engineers of Korea SD
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    • v.49 no.2
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    • pp.47-55
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    • 2012
  • A discrete-time(DT) filter with high-order temporal moving average(TMA) using actively-weighted charge sampling is proposed in this paper. To obtain different weight of sampled charge, the variable transconductance OTA is used prior to charge sampler, and the ratio of charge can be effectively weighted by switching the control transistors in the OTA. As a result, high-order TMA operation can be possible by actively-weighted charge sampling. In addition, the transconductance generated by the OTA is relatively accurate and stable by using the size ratio of the control transistors. The high-order TMA filter has small size, increased voltage gain, and low parasitic effects due to the small amount of switches and sampling capacitors. It is implemented in the TSMC $0.18-{\mu}m$ CMOS process by TMA-$2^2$. The simulated voltage gain is about 16.7 dB, and P1dB and IIP3 are -32.5 dBm and -23.7 dBm, respectively. DC current consumption is about 9.7 mA.

Principles and Current Trends of Neural Decoding (뉴럴 디코딩의 원리와 최신 연구 동향 소개)

  • Kim, Kwangsoo;Ahn, Jungryul;Cha, Seongkwang;Koo, Kyo-in;Goo, Yong Sook
    • Journal of Biomedical Engineering Research
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    • v.38 no.6
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    • pp.342-351
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    • 2017
  • The neural decoding is a procedure that uses spike trains fired by neurons to estimate features of original stimulus. This is a fundamental step for understanding how neurons talk each other and, ultimately, how brains manage information. In this paper, the strategies of neural decoding are classified into three methodologies: rate decoding, temporal decoding, and population decoding, which are explained. Rate decoding is the firstly used and simplest decoding method in which the stimulus is reconstructed from the numbers of the spike at given time (e. g. spike rates). Since spike number is a discrete number, the spike rate itself is often not continuous and quantized, therefore if the stimulus is not static and simple, rate decoding may not provide good estimation for stimulus. Temporal decoding is the decoding method in which stimulus is reconstructed from the timing information when the spike fires. It can be useful even for rapidly changing stimulus, and our sensory system is believed to have temporal rather than rate decoding strategy. Since the use of large numbers of neurons is one of the operating principles of most nervous systems, population decoding has advantages such as reduction of uncertainty due to neuronal variability and the ability to represent a stimulus attributes simultaneously. Here, in this paper, three different decoding methods are introduced, how the information theory can be used in the neural decoding area is also given, and at the last machinelearning based algorithms for neural decoding are introduced.

LED lighting control system using the variable FOV according to movements of stage actors based on multi sensor (멀티센서기반 무대배우 이동에 따른 FOV가변형 LED조명 제어 시스템)

  • Koo, EunJa;Cha, Jaesang;Kim, Daeho;Park, Myungsook
    • Journal of Satellite, Information and Communications
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    • v.7 no.3
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    • pp.16-21
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    • 2012
  • Recently, an importance of culture industry has been emphasized through an increased income level, spare time and changed values of modern people. And demands of the performance, arts, exhibit are steadily being increased. However the stage equipment depends on foreign manufactures on account of the inactive domestic technical skills. Especially in the lighting direction part, it is essential to control the lighting source and detect the moving line of actors but it generally uses the manual control type and realization of actor's moving line regardless of existing IT-based technologies. Also the system operation of existing sensor-based tracking and detecting technologies depends on the main lighting source of the stage. Therefore, this paper proposed LED lighting control system using the variable FOV and multi sensor-based tracking algorithm, which are possible to efficiently track the stage actors and direct the stage lights. Also we demonstrated the practicality and possibility of realization through the integrated experiment of the proposed system and implementation of the salient hardware, software. Additionally, the usefulness of proposed system was demonstrated using performance simulations and actual measurements of implemented sensor output.

Optimal Design of Network-on-Chip Communication Sturcture (Network-on-Chip에서의 최적 통신구조 설계)

  • Yoon, Joo-Hyeong;Hwang, Young-Si;Chung, Ki-Seok
    • Journal of the Institute of Electronics Engineers of Korea SD
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    • v.44 no.8
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    • pp.80-88
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    • 2007
  • High adaptability and scalability are two critical issues in implementing a very complex system in a single chip. To obtain high adaptability and scalability, novel system design methodology known as communication-based system design has gained large attention from SoC designers. NoC (Network-on-Chip) is such an on-chip communication-based design approach for the next generation SoC design. To provide high adaptability and scalability, NoCs employ network interfaces and routers as their main communication structures and transmit and receive packetized data over such structures. However, data packetization, and routing overhead in terms of run time and area may cost too much compared with conventional SoC communication structure. Therefore, in this research, we propose a novel methodology which automatically generates a hybrid communication structure. In this work, we map traditional pin-to-pin wiring structure for frequent and timing critical communication, and map flexible and scalable structure for infrequent, or highly variable communication patterns. Even though, we simplify the communication structure significantly through our algorithm the connectivity or the scalability of the communication modules are almost maintained as the original NoC design. Using this method, we could improve the timing performance by 49.19%, and the area taken by the communication structure has been reduced by 24.03%.

A Study on the Design and Fabrication of Phase Locked Dielectric Resonance Oscillator (위상고정 유전체 공진형 발진기의 설계 및 제작에 관한 연구)

  • Seo Gon;Park hang-Hyun;Kim Jang-Gu;Choi Byung-Ha
    • Journal of the Institute of Electronics Engineers of Korea TC
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    • v.42 no.3 s.333
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    • pp.25-32
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    • 2005
  • In this papers, we first, therefore, designed VCO(voltage controlled oscillator) that is composed of the dielectric resonator and the varactor diode, and then designed and fabricated PLDRO(phase locked dielectric resonator oscillator) that is combined with the sampling phase detector and loop filter. The measured results of the fabricated PLDRO at 12.05 [GHz] show the output power is 13.54 [dBm], frequency tuning range approximately +/- 7.5 [MHz], and Power variation over the tuning range less than 0.2 [dB], respectively. The phase noise which effects on bits error rate in digital communication is obtained with -114.5 [dBc/Hz] at 100 [KHz] offset from carrier, and The second harmonic suppression is less than -41.49 [dBc]. These measured results are found to be more improved than those of VCO without adopting PLL, and the phase noise and power variation performance characteristics show the better performances than those of conventional PLL.

Predicting the Suitable Habitat of Amaranthus viridis Based on Climate Change Scenarios by MaxEnt (MaxEnt를 활용한 청비름(Amaranthus viridis)의 기후변화 시나리오에 의한 서식지 분포 변화 예측)

  • Lee, Yong Ho;Hong, Sun Hee;Na, Chae Sun;Sohn, Soo In;Kim, Myung Hyun;Kim, Chang Seok;Oh, Young-Ju
    • Korean Journal of Environmental Biology
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    • v.34 no.4
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    • pp.240-245
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    • 2016
  • This study was conducted to predict the changes of potential distribution for invasive alien plant, Amaranthus viridis in Korea. The habitats of A. viridis were roadside, bare ground, farm area, and pasture, where the interference by human was severe. We used maximum entropy modeling (MaxEnt) for analyzing the environmental influences on A. viridis distribution and projecting on two different representative concentration pathways (RCP) scenarios, RCP 4.5 and RCP 8.5. The results of our study indicated annual mean temperature, elevation and precipitation of coldest month had higher contribution for A. viridis potential distribution. Projected potential distribution of A. viridis will be increased by 110% on RCP 4.5, 470% on RCP 8.5.