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Identification of a Major QTL, qSTV11SG, Associated with Resistance to Rice Stripe Virus Disease Originated from Shingwangbyeo in Rice (Oryza Sativa L.) (신광벼 유래의 벼 줄무늬잎마름병 저항성 주동 QTL qSTV11SG탐색)

  • Kwak, Do-Yeon;Lee, Bong-Chun;Choi, Ilyoung;Yeo, Un-Sang;Cho, Jun-Hyun;Lee, Ji-Yoon;Song, You-Chun;Yun, Yeong-Nam;Park, Dong-Soo;Kang, Hang-Won;Nam, Min-Hee;Lee, Jong-Hee
    • Korean Journal of Breeding Science
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    • v.43 no.5
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    • pp.464-469
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    • 2011
  • Virus diseases often cause serious damage to rice production in Asia. The lack of information on virus resistance genes has been a major obstacle for the breeding of resistant varieties. In order to identify DNA marker associated with resistance against rice stripe virus (RSV), the quantitative trait locus (QTL) was carried out using advanced backcross population developed from a cross between RSV-resistant tongil type cultivar Shinkwang and susceptible japonica cultivar Ilpum. A RSV resistance QTL $qSTV11^{SG}$ explaining 44.2% of the phenotypic variation was identified on chromosome 11 of Tongil type rice cultivar 'Shingwang'. $qSTV11^{SG}$ was tightly linked to DNA marker RM6897. The RM6897 divided as resistance type allele and susceptible type alleles. Twenty seven resistant varieties showed the resistant-type allele and 23 susceptible varieties were susceptible-type allele to the marker of RM6897. This results and the molecular markers presented here may be useful in rice breeding for improving RSV resistance in japonica rice.

Variation of Nuclear DNA Content in Interspecific Allium cepa L.×A. fistulosum L. hybrids and Their Successive Backcross Lines (양파와 파간의 종간잡종 F1과 여교잡계통의 핵 DNA 함량)

  • Kim, Cheol-Woo;Kim, Hwa-Young;Lee, Eul-Tai;Choi, In-Hu;Bang, Jin-Ki
    • Korean Journal of Breeding Science
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    • v.41 no.4
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    • pp.463-467
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    • 2009
  • Interspecific hybrid plants between Allium cepa L. (2n=2X=16) and A. fistulosum L. (2n=2X=16)and their backcross lines were developed by artificial pollination in order to introduce new desirable characters of A, cepa to A. fistulosum. The 2C nuclear DNA content has been estimated by flow cytometry in 5 Allium fistulosum inbreed lines, 2 interspecific hybrid lines of A. cepa${\times}$A. fistulosum and 34 their backcross lines $BC_1F_1$ to $BC_2F_2$, using propidium iodide (PI) as a fluorescence dye. Estimated 2C DNA values ranged from 22.2 pg to 23.7 pg in 5 A. fistulosum inbreed lines, 37.9 pg in F1 hybrid between A. cepa and A. fistulosum, 24.3 pg to 27.3 pg in 7 backcross lines in $BC_1F_1$, 21.9 pg to 24.4 pg in 9 $BC_1F_2$, 22.9 pg to 25.1 pg in 14 $BC_2F_1$, 22.6 pg to 23.4 pg in 4 $BC_2F_2$. This study showed mean 2C nuclear DNA content of $F_1$ hybrid was higher than their backcross progeny lines, while it was lower than female parental line, A. cepa (2C DNA=33.2 pg). Mean 2C DNA content of backcross lines, $BC_1F_1$ to $BC_2F_2$ was not significantly different but their 2C DNA contents in the more progress generation from $BC_1F_1$ to $BC_2F_2$ were reduced.

A study of the Implications of French vocabularies and the de-locality in LEE Sang's Poems (이상(李箱)의 시 작품에 구사되는 프랑스어와 탈 지방성)

  • Lee, Byung-soo
    • Cross-Cultural Studies
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    • v.53
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    • pp.1-24
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    • 2018
  • This following research is a study on the use of French and de-locality in the modern Korean poet Lee Sang's poetry (1910-1937). His hometown was Kyung Sung, Seoul. He mainly wrote his works in Korean, Chinese character, and Japanese, using the language of education and his native language at that time. So then, what was the spirit that he wanted to embody through use of French words? By using words like "ESQUISSE", "AMOUREUSE", Sang's French was not a one-time use of foreign words intended to amuse, but to him the words were as meticulously woven as his intentions. French words were harmonized with other non-poetic symbols such as "${\Box}$, ${\triangle}$, ${\nabla}$", and described as a type of typographical hieroglyphics. Instead of his mother-tongue language, French was applied as a surrealistic vocabulary that implemented the moral of infinite freedom and imagination, and expressed something new or extrasensory. Subsequently, the de-localized French (words) in his poetry can be seen as poetic words to implement a "new spirit", proposed by western avant-garde artists. Analysis of French in his poetry, showed a sense of yearning for the scientific civilization, calling for his sense of defeat and escape from the colonized inferior native land. Most of all, comparing his pursuit of western civilization and avant-garde art to French used in his poetry, is regarded as world-oriented poetry intended to implement the new tendency of the "the locomotive of modernity," transcending the territory of the native country.

Analysis of Quantitative Trait Loci (QTL) Associated with the Gel Consistency in Rice (쌀의 호응집성에 대한 QTLs 분석)

  • Kim, Tae-Heon;Sohn, Jae-Keun;Kim, Kyung-Min
    • Korean Journal of Breeding Science
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    • v.41 no.4
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    • pp.474-481
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    • 2009
  • The purpose of this study was to identify the quantitative trait loci (QTL) associated with gel consistency (GC), and to evaluate the relationships between GC and physico-chemical properties related to eating quality in rice. A total of 120 doubled haploid (DH) lines derived from a cross between 'Samgang' and 'Nagdong' and 26 varieties were used for this study. The 120 DH lines were divided into four groups, soft (61~100 mm), medium (41~60 mm), hard (26~40 mm) and whole population, according to the gel length. A negative correlation between GC and amylose content was observed only in the soft group. The GC negatively correlated with lipid content in the medium, and the whole population, but positively correlated with lipid content in the soft group. The positive correlation between GC and protein content was observed in the soft group, while negative correlations were observed in the medium group and the whole population. Two significant QTLs regarding GC were detected on chromosomes 4 and 11. They collectively explained 23% of phenotypic variation with LOD score of 3.1 and 2.9, respectively. The DNA markers of S4026 and RM287 were tightly linked to GC in the DH population and 26 varieties, respectively.

Mapping Grain Weight QTL using Near Isogenic Lines from an Interspecific Cross (벼 종간잡종 유래 근동질 유전자계통 이용 종자중 관여 유전자 분석)

  • Kang, Ju-Won;Yang, Paul;Yun, Yeo-Tae;Ahn, Sang-Nag
    • Korean Journal of Breeding Science
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    • v.43 no.4
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    • pp.304-310
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    • 2011
  • In previous studies, we reported QTLs for grain weight (GW), qGW3 and for spikelets per panicle (SPP), qSPP3 linked to RM60 on chromosome 3 using advanced backcross lines derived from a cross between Oryza sativa ssp. Indica cv. Milyang 23 and O. glaberrima. The O. glaberrima alleles at this locus increased GW and spikelets per panicle in the Milyang 23 background. To further confirm and narrow down the position of the QTLs on chromosome 3, substitution mapping was performed using five lines containing the target O. glaberrima segment on chromosome 3. The size and position of the O. glaberrima segment on chromosome 3 were different in each line. These lines possessed 3-10 non-target O. glaberrima introgressions in the Milyang 23 background. These five lines were evaluated for seven agronomic traits including 1,000 grain weight and spikelets per panicle and also genotyped with seven SSR markers. Four lines were informative in delimiting the position of QTLs, qGW3 and qSPP3. Two lines with the O. glaberrima segment flanked by SSR markers, RM60 and RM523 displayed significantly higher values than Milyang 23 in GW and SPP whereas two lines without that O. glaberrima segment displayed no difference in GW and SPP compared to Milyang 23. The result indicates that two QTL, qGW3 and qSPP3 are located in the interval between RM60 and RM523 which are 1.2-Mb apart. Introgression lines having QTLs, qGW3 and qSPP3 would be useful materials not only to indentify the relationship between these two yield QTLs, but also to develop high yielding variety via marker-aided selection technology.

Emotional Labor between Service Job vs. Non-Service Job and Effect of Emotional Labor on Depression and quality of Life (서비스직과 비서비스직의 감정노동 및 감정노동이 우울과 삶의 질에 미치는 영향)

  • Kim, Hwan;Han, Sumi;Choi, Hyera
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.20 no.4
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    • pp.177-188
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    • 2019
  • Emotional labor is the process of regulating feelings or emotions and expressing them in the way that fulfill job requirement. There have been many studies about characteristics or related variables of service workers' emotional labor, but are few studies comparing emotional labor between service workers and non-service workers. Therefore we are to examine the differences of emotional labor among the different types of workers. And as depression and lowered quality of life are well known negative consequences of emotional labor, we also intend to study the relationship between depression, quality of life, and emotional labor. Data were collected from 125 sales workers, and 186 cyber university full time students. And as to assure the student participants to be non-service workers, we limited the participant job as administrator, soldier or housewife. To compare differences of groups, one-way ANOVA was performed with Fisher's LSD as post hoc comparison. On overload in customer reception, service workers showed significantly higher scores, and on demand of emotional regulation/emotional dissonance/depression, both service workers and housewives showed significantly higher scores. Also analysis of multiple regression was performed, and the result showed that, emotional dissonance increased depression but decreased quality of life, while support/care increased quality of life, but decreased depression. With the result, implications and limitations of this study were discussed.

Construction of Yeast Strain Suitable for Bioethanol Production by Using Fusion Method (융합법을 이용한 바이오에탄올 생산에 적합한 효모균주의 구축)

  • Kim, Yeon-Hee
    • Journal of Life Science
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    • v.29 no.3
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    • pp.376-381
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    • 2019
  • To construct useful yeast strain for bioethanol production, we improved yeast harboring various phenotypes by using yeast protoplast fusion method. In this study, S. cerevisiae BYK-F11 strain which have ethanol tolerance, thermotolerance and ${\beta}-glucanase$ activity and P. $stipitis{\Delta}ura$ strain which has xylose metabolism pathway were fused by genome shuffling. P. $stipitis{\Delta}ura$ strain was constructed for protoplast fusion by URA3 gene disruption, resulting in uracil auxotroph. By protoplast fusion, several fused cells were selected and BYKPS-F8 strain (fused cell) showing both karyotypes from two parent strains (S. cerevisiae BYK-F11 and P. $stipitis{\Delta}ura$ strain) among 22 fused cells was finally selected. Sequentially, various phenotypes such as ${\beta}-glucanase$ activity, xylose utility, ethanol tolerance, thermotolerance and ethanol productivity were analyzed. The BYKPS-F8 strain obtained ${\beta}-glucanase$ activity from BYK-F11 strain and 1.2 fold increased xylose utility from P. $stipitis{\Delta}ura$ strain. Also, the BYKPS-F8 strain showed thermotolerance at $40^{\circ}C$ and increased ethanol tolerance in medium containing 8% ethanol. In this fused cell, 7.5 g/l ethanol from 20 g/l xylose was produced and the multiple phenotypes were stably remained during long term cultivation (260 hr). It was proved that novel biological system (yeast strains) is easily and efficiently bred by protoplast fusion among yeasts having different genus.

Assessment of Equivalent Heights of Soil for the Lateral Earth Pressure Against Retaining Walls Due to Design Truck Load (표준트럭하중에 의해 옹벽에 작용하는 수평토압의 등가높이 산정)

  • Kim, Duhwan;Jin, Hyunsik;Seo, Seunghwan;Park, Jaehyun;Kim, Dongwook;Chung, Moonkyung
    • Journal of the Korean Geosynthetics Society
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    • v.17 no.4
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    • pp.119-128
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    • 2018
  • Limit state design has been implemented in Korea since 2015; however, there exists no specification of lateral load determination on retaining wall due to the Korean standard traffic load on retaining wall's backfill surface. The lateral load from traffic depends on lane number, standard truck's axle loads and locations, loading distance from the inner wall. The concept of equivalent height of soil accounting for traffic loadings is typically used for design of retaining walls to quantify the traffic loads transmitted to the inner wall faces. Due to the different characteristics of the standard design trucks between Korea and US (AASHTO), the direct use of the guidelines from AASHTO LRFD leads to incorrect estimation of traffic load effects on retaining walls. This paper presents the results of evaluation of equivalent height of soil to reflect the Korean standard truck, based on the findings from analytical solutions using Bounessq's theory and numerical assessment using 2D finite element method. Consequently, it was found that the equivalent heights of soil from the Korean standard truck load were lower for lower retaining wall height.

Development of Construction Manager's Competency Evaluation Index Using Sports Analysis Techniques - Focused on Notational Analysis - (스포츠 분석 기법을 활용한 건설관리자 역량 평가지표 개발 - 부호화 분석을 중심으로 -)

  • Lee, Jinsol;Park, Moonseo;Lee, Hyeon-soo;Cho, Jongwoo;Yoon, Inseok
    • Korean Journal of Construction Engineering and Management
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    • v.20 no.4
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    • pp.34-45
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    • 2019
  • To drive construction project successful, a construction manager with good competency is needed. In order to select appropriate construction manager, there should be a standard and a method for appropriately evaluating the competency of construction manager. There have been many researches on the competency of construction managers, but there are two points to be supplemented. One is that construction manager's competency measurement was not concrete and objective, and the other is that construction project performance directly reflects individual manager's performance. The purpose of this study is to derive a construction manager competency evaluation index by using the Notational Analysis technique that is used when deriving an indicator that measures the athlete's competency in sports. This analysis was drawn with Delphi method. Finally, the 'measurable behaviors'were derived that can evaluate the competency of construction managers. Through this, it is possible to express each manager's expertise, leadership, and personal characteristics in numerical expression. This will help decision makers when selecting construction managers. It is anticipated that decision makers will be able to make the decision to select the necessary construction managers easier because it can evaluate the construction managers through a scientific approach based on the record by supplementing the existing method which only relied on intuition.

Categorization of Citiesin Gyeonggi-do Using Ecosystem Service Bundles (생태계서비스 번들을 이용한 경기도 도시의 유형화)

  • Kim, Ilkwon;Kim, Sunghoon;Lee, Jooeun;Kwon, Hyuksoo
    • Journal of Environmental Impact Assessment
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    • v.28 no.3
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    • pp.201-214
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    • 2019
  • The concept of ecosystem services is important for the effective management of regional ecological resources. Multiple ecosystem services provided by regional ecosystems are represented as ecosystem service bundles, which define the co-occurrent ecosystem services in a specific region. Bundles provide useful information to identify regional characteristics of ecosystem services and categorize sub-regions with similar patterns of ecosystem service provision. We assessed eleven ecosystem services using modeling approaches and statistical data and produced bundles of cities in Gyeonggi-do.We also conducted principal component analysis and cluster analysis to categorize these cities according to the characteristics of ecosystem services. The results indicated that the cities in Gyeonggi-do were categorized into three groups depending on the types of provision,regulation, and cultural services, and were designated as urbanized, urban-forest, agriculture, or forest cities. These groups were influenced by land use patterns reflecting regional social-environmental features. The results provide useful information for identifying regional ecosystem services and facilitate decision-making in regional ecosystem service management.