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A Study on the Cytotoxicity of Lead in Cultured Fibroblasts (납(Pb)이 배양 섬유모세포에 미치는 세포독성에 관한 연구)

  • 정연태;최민규;김정중;문연자;김재민;백순기
    • Environmental Mutagens and Carcinogens
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    • v.15 no.2
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    • pp.122-130
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    • 1995
  • In order to evaluate the cytotoxicity of lead in cultures of Balb/c mouse 3T3 cell line, various cytotoxic assays were carried out after expose cells to various concentrations of lead nitrate. Cytotoxic assays using this study were included NR assay, MTT assay, measurement of LDH and protein, synthetic rate of DNA and UDS. Intrace!!ular Ca$^{2+}$ level was also measured. Light and electron microscopic studies were done for morphological changes of lead-treated cell cultures. The results were as follows; 1. The absorbances of NR and MTT were decreased dose-dependently, and NR, and MTT, values of lead nitrate were 3.4 mM and 1.5 mM, respectively. 2. Amount of LDH released into the medium was increased in dose-dependently and LDH activity at 5 mM concentration of lead nitrate was increased to 335 % of control. 3. Amount of total protein was decreased dose-dependently, and which was half of control at 2 mM concentration of lead nitrate. 4. The synthetic rate of DNA was decreased dose-dependently, and also which was remarkably decreased at 3 mM and 5 mM concentrations of lead nitrate. 5. The synthetic rate of UDS was increased at 1 mM concentration of lead nitrate, but which was remarkably decreased at 3 mM and 5 mM concentrations of lead nitrate. 6. Intrace!lular Ca$^{2+}$ level was remarkably increased at 1 mM concentration of lead nitrate, compared with control. 7. In light microscopy, number of cells and processes were decreased according to the increase of dosage of lead nitrate. Electron microscopic findings showed that many vacuoles and cisternal dilatation of rough endoplasmic reticulum were seen in the cytoplasm at 1 mM concentration of lead nittale. From the above results, high dosage treatment of lead nitrate (>3 mM) damaged genetic malerials and it also showed cytotoxicity in mouse 3T3 cell line cultures by injury of cell organelles and Ca$^{2+}$ channel.

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Effects of Ethyl methanesuifonate and Ultraviolet light on Induction of the Adaptive Response in Chinese Hamster Ovary and Sarcoma 180 Cells

  • Kim, Gyoo-Cheon;Lee, Dong-Wook;Shin, Eun-Joo;Um, Kyung-Il
    • Environmental Mutagens and Carcinogens
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    • v.16 no.1
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    • pp.19-23
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    • 1996
  • This study was performed by the sister chromatid exchanges (SCEs) and micronuclei (MN) assays to investigate the adaptive response to ultraviolet light (UV) or ethyl methanesulfonate (EMS) in Chinese hamster ovary (CHO) and Sarcoma 180 (S180) cells. The pretreatment with 1 J/m$^2$ UV or 2 mM EMS decreased the frequency of SCEs induced by the treatment with 5 J/m$^2$ UV or 8 mM EMS in CHO cells. The pretreatment with UV (1 or 2 J/m$^2$) or EMS (1, 2 or 3 mM) did not affect the SCEs induced by the treatment with 7 J/m$^2$ UV or 10 mM EMS in S180 cells. On the other hand, the pretreatment with 1 J/m$^2$ UV or 2 mM EMS decreased the frequency of MN induced by the treatment with 5 J/m$^2$ UV or 8 mM EMS in CHO cells. The pretreatment with UV (1 or 2 J/m$^2$) or EMS (1, 2 or 3 mM) did not affect the frequency of MN induced by the treatment with 7 J/m$^2$ UV or 10 mM EMS in S180 cells. It is suggested that there are adaptive responses at the level of chromosome and micronuclei to UV and EMS in CHO cells.

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Effects of Root-knot Nematodes, Meloidogyne hapla, M. incognita, M. arenaria and M. javanica on Growth and Yield of Tomato (토마토에 대한 뿌리혹선충의 주요 종별 피해정도)

  • Cho H. J.;Kim C. H.;Park J. S.;Jeoung M. G.
    • Korean Journal Plant Pathology
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    • v.3 no.3
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    • pp.164-167
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    • 1987
  • The effects of infection severity of susceptible tomato varieties, Rutgers and Boksu 2, by root-knot nematode, meloidogyne hapla, M. arenaria or M. javanica on plant growths and tomato yields were investigated. The inoculum levels of each nematode species were 0, 1,000 and 10,000 nematodes per 42cm diam. pot. Tomato yield was reduced by the nematode infection. Severity of infection was on the decreasing order of M. javanica, M. incognita, M. arenaria, M. hapla, ranging from $80\%$ by M. javanica to only $7\%$ by M. hapla. Yield reduction by infection of M. hapla was more prominent in Boksu 2 than in Rutgers. However the results were vice versa for the other nematode species, The top fresh-weight of Rutgers inoculated with 10,000 nematodes was greater than root weight, regardless of the nematode species, whereas plant height and top fresh weight decreased with increasing root weight when inoculated with inoculum density of 1,000/pot.

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Design and Implementation of M2M Platform based on PWW (PWW 기반 사물통신 플랫폼의 설계 및 구현)

  • Choi, Bokdong;Eun, Seong Bae;Kim, Byungho
    • Journal of the Korea Institute of Information and Communication Engineering
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    • v.17 no.3
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    • pp.740-746
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    • 2013
  • Machine to machine (M2M) communication is a converged ICT(Information and Communications Technology) platform which can utilize communications services among people and things anytime, any place in realtime. The M2M platform consists of three components; M2M area networks sensing and generating data of things by various sensors and devices; public networks transporting those data; and M2M applications utilizing the aggregated data. There exist however two practical obstacles to realize the M2M communications in terms of interoperability between the WCDMA-based public network and the local networks based on W-Fi or ZigBee; one is the cost problem required to access the public network, and the other is the lack of a cost-effective modem or gateway to connect two different networks. In this paper, we proposed and implemented a novel M2M platform based on PWW (Person Area Network) which can resolve those two issues by using a proposed gateway connecting the local network and smartphones as application devices without access the WCDMA-based public network. We have shown that the proposed platform is very practical to adopt to the real environment by actual testing with the own developed smartphone applications.

RELATIVE AGE DIFFERENCE BETWEEN THE METAL-POOR GLOBULAR CLUSTERS M53 AND M92

  • CHO, DONG-HWAN;SUNG, HYUN-IL;LEE, SANG-GAK;YOON, TAE SEOG
    • Journal of The Korean Astronomical Society
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    • v.49 no.5
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    • pp.175-192
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    • 2016
  • CCD photometric observations of the globular cluster (GC), M53 (NGC 5024), are performed using the 1.8 m telescope at the Bohyunsan Optical Astronomy Observatory in Korea on the same nights (2002 April and 2003 May) as the observations of the GC M92 (NGC 6341) reported by Cho and Lee using the same instrumental setup. The data for M53 is reduced using the same method as used for M92 by Cho and Lee, including preprocessing, point-spread function fitting photometry, and standardization etc. Therefore, M53 and M92 are on the same photometric system defined by Landolt, and the photometry of M53 and M92 is tied together as closely as possible. After complete photometric reduction, the V versus B − V , V versus V − I, and V versus B − I color-magnitude diagrams (CMDs) of M53 are produced to derive the relative ages of M53 and M92 and derive the various characteristics of its CMDs in future analysis. From the present analysis, the relative ages of M53 and M92 are derived using the Δ(B − V ) method reported by VandenBerg et al. The relative age of M53 is found to be 1.6 ± 0.85 Gyr younger than that of M92 if the absolute age of M92 is taken to be 14 Gyr. This relative age difference between M53 and M92 causes slight differences in the horizontal-branch morphology of these two GCs.

ON ENDOMORPHISM RING OF H-INVARIANT MODULES

  • Bae, Soon-Sook
    • East Asian mathematical journal
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    • v.6 no.2
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    • pp.167-182
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    • 1990
  • The relationships between submodules of a module and ideals of the endomorphism ring of a module had been studied in [1]. For a submodule L of a moudle M, the set $I^L$ of all endomorphisms whose images are contained in L is a left ideal of the endomorphism ring End (M) and for a submodule N of M, the set $I_N$ of all endomorphisms whose kernels contain N is a right ideal of End (M). In this paper, author defines an H-invariant module and proves that every submodule of an H-invariant module is the image and kernel of unique endomorphisms. Every ideal $I^L(I_N)$ of the endomorphism ring End(M) when M is H-invariant is a left (respectively, right) principal ideal of End(M). From the above results, if a module M is H-invariant then each left, right, or both sided ideal I of End(M) is an intersection of a left, right, or both sided principal ideal and I itself appropriately. If M is an H-invariant module then the ACC on the set of all left ideals of type $I^L$ implies the ACC on M. Also if the set of all right ideals of type $I^L$ has DCC, then H-invariant module M satisfies ACC. If the set of all left ideals of type $I^L$ satisfies DCC, then H-invariant module M satisfies DCC. If the set of all right ideals of type $I_N$ satisfies ACC then H-invariant module M satisfies DCC. Therefore for an H-invariant module M, if the endomorphism ring End(M) is left Noetherian, then M satisfies ACC. And if End(M) is right Noetherian then M satisfies DCC. For an H-invariant module M, if End(M) is left Artinian then M satisfies DCC. Also if End(M) is right Artinian then M satisfies ACC.

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Serum 25-Hydroxy Vitamin $D_3$ Analysis of Korean People (한국인 일반인의 혈청 25-Hydroxy Vitamin $D_3$의 분석)

  • Kim, Bo-Kyung;Jung, Hyun-Mi;Kim, Yun-Kyung;Kim, So-Young;Kim, Jee-Hyun
    • The Korean Journal of Nuclear Medicine Technology
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    • v.14 no.1
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    • pp.133-137
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    • 2010
  • Purpose: The main function of vitamin D is the mineralization of the brain by increase of calcium and phosphorus, in case it is insufficient in children, lime deposition on cartilage cannot occur so it leads to rachitis, and in adults, it leads to osteomalacia or osteoporosis. It is also strongly believed in the academic world that vitamin D can restrict the growth of cancer cells and prevent heart diseases, which is also somewhat proven in epidemiological researches. While the right density of vitamin D is still being studied, 20-32 ng/mL is believed to be the most ideal density. Therefore, I wanted analyze how much density of 25-Hydroxyvitamin D3 that Koreans possess. Materials and Methods: From February 20th, 2008 to April 21st, 2009, the collection of 2800 serums, from medical examination treated subjects by Neodin Medical Institute, have been tested. The targets were tested by 25-Hydroxyvitamin D (125I Kit: Diasorin, USA), and were analyzed by dividing into many different categories (gender, age, season, region). Results: The average density of male were 20 ng/mL, female 17.08 ng/mL. Per age groups, the density of males were as follows: 10~20-18 ng/mL, 21~30-17 ng/mL, 31~40-19 ng/mL, 41~50-21 ng/mL, 51~60-22 ng/mL, 61~70-22 ng/mL, 71~80-22 ng/mL and 81~90-19.9 ng/mL. Average density of females per age groups, were as follows: 10~20-16 ng/mL, 20~30-15.26 ng/mL, 30~40-16 ng/mL, 40~50-17 ng/mL, 50~60-19 ng/mL, 60~70-19 ng/mL, 70~80-19 ng/mL, and 80~90-17 ng/mL. Per seasons, From December to May, the subjects showed the density of 15.97 ng/mL, while from June to November, it showed 21.60 ng/mL. On density of males from January to April regionally, Seoul+Gyeonggi-Do-15.52 ng/mL, Gangwon-Do-15.33 ng/mL, Choongchung-Do-18.03 ng/mL, Jeonla-Do-18.68 ng/mL, Gyungsang-Do-18.76 ng/mL and Cheju Do-21.23 ng/mL. Conclusions: The vitamin D of Koreans is has been insufficient compared to the suggested amount. Ultraviolet rays, which is the main source of vitamin D is critical, therefore it is suggested that more outdoor activities can definitely help.

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A Research of the suitable Area and Module in Elementary School Classroom - Focusing on Elementary Schools of Northern Province of Gyeonggi-do - (초등학교 일반교실의 필요 면적과 모듈에 관한 연구 - 경기북부지역 초등학교를 중심으로-)

  • Yoon, Hee-Cheol
    • The Journal of Sustainable Design and Educational Environment Research
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    • v.20 no.3
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    • pp.1-10
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    • 2021
  • Before long the number of Elementary School students per class will be 20 in Korea, but the current Area and Module of Elementary School are planned for 30 students. Therefore, necessary Area and Module for less than 20 students should be prepared. The purpose of this research is to find out necessary Area and Module for less than 20 students in Elementary School Classroom. I researched 60 Classrooms of 10 Elementary Schools before 2 researches of mine, and researched the sizes of every path in the classrooms. With the Plans for 20 students, I found the conclusion as follows: First, the one-way class requires a minimum of 5.4m×8.1m (43.74m2), a maximum of 5.4m×8.7m (48.6m2). Second, the 3-row alignment class requires a minimum of 7.2m×7.2m (51.84m2), a maximum of 7.5m×7.5m (56.25m2). Third, the group study class requires a minimum of 6.0m×8.7m (52.2m2), a maximum of 6.3m×9.3m (58.59m2). Fourth, the group study class requires a minimum of 2.34m2, a maximum of 14.85m2 more than the one-way class. Fifth, the suitable module which fits both 2-row alignment class and group study class except the 3-row alignment class is 6.0m×8.7m (52.2m2).

ON M-CONTINUITY

  • Min, Won Keun;Chang, Hong Soon
    • Korean Journal of Mathematics
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    • v.6 no.2
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    • pp.323-329
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    • 1998
  • In this paper, we introduce a new class of sets, called $m$-sets, and the notion of $m$-continuity. In particular, $m$-sets and $m$-continuity are used to extend known results for ${\alpha}$-continuity and semi-continuity and precontinuity.

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ON MINIMAL PRECONTINUOUS FUNCTIONS

  • Min, Won Keun;Kim, Young Key
    • Journal of the Chungcheong Mathematical Society
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    • v.22 no.4
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    • pp.667-673
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    • 2009
  • In this paper, we introduce the notions of minimal precontinuity, m-preclosed graph, almost m-precompact and m-precompact and investigate some properties for such notions.

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