• Title/Summary/Keyword: $alpha$-glucosidase inhibitory activity

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Antioxidant and α-Glucosidase Inhibition Activity of Solvent Fractions from Prunus mume Ethanol Extract (매실 순차분획물의 용매별 항산화 활성 및 α-glucosidase 억제 효과)

  • Kim, Jeong-Ho;Cho, Hyun-Dong;Won, Yeong-Seon;Park, Wool-Lim;Min, Hye-Ji;Han, Sim-Hee;Moon, Kwang-Deog;Seo, Kwon-Il
    • Journal of Life Science
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    • v.29 no.10
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    • pp.1111-1119
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    • 2019
  • Prunus mume, known as maesil in Korea, has been widely cultivated in East Asia and used as medication and food. However, because most of the previous studies concerning P. mume had been investigated its under extract state, detailed studies are still required for its extensive utilization. In this study, we evaluated the antioxidant and ${\alpha}-glucosidase$ inhibitory activities of solvent fractions of P. mume ethanol extracts. The ethyl acetate fraction showed higher DPPH radical scavenging activity, ABTS radical scavenging activity, reducing power, and hydrogen peroxide scavenging activity than other fractions. The DPPH radical scavenging activities of ethyl acetate fraction was 67.79%; ABTS radical scavenging activity was 60.03%; reducing power ($OD_{670}$) was 1.26; and hydrogen peroxide scavenging activity was 93.18% at $500{\mu}g/ml$. Also, the ethyl acetate and methanol fraction showed effective levels of ${\alpha}-glucosidase$ inhibition activity (69.25% and 72.29% at $500{\mu}g/ml$). Total polyphenol contents and total flavonoid contents of the ethyl acetate fraction were 88.28 mg/g (gallic acid equivalent) and 70.38 mg/g (quercetin equivalent), respectively. These results suggest that the physiological activities of the ethyl acetate fraction are associated with its polyphenol and flavonoid contents. Therefore, this study can be used as basic data for developing natural antioxidants and potential functional material using P. mume.

Antioxidative and digestion enzyme inhibitory activity of Ganoderma lucidum depends on the extraction solvent (추출용매에 따른 영지버섯(Ganoderma lucidum)의 항산화 및 소화효소 저해활성)

  • Joo, Ok Soo;Hwang, Chung Eun;Hong, Su Young;Sin, Eui Cheol;Nam, Sang Hae;Cho, Kye Man
    • Food Science and Preservation
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    • v.25 no.1
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    • pp.124-135
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    • 2018
  • This study investigated the nutritional properties and biological activities of Ganoderma lucidum (GL). The round type of GL contained higher carbohydrate content, while the Nokgak type of GL contained higher crude ash, crude fat, and crude protein content. The most abundant amino acid, fatty acid, mineral, and soluble vitamin observed were valine (round type: 11.90 mg/g and Nokgak type: 17.18 mg/g), linoleic acid (round type: 47.56% and Nokgak type: 75.68%), potassium (round type: 116.50 mg/100 g and Nokgak type: 184.36 mg/100 g), and vitamin B3 (round type: 1.78 mg/100 g and Nokgak type: 1.81 mg/100 g), respectively. In addition, the ${\beta}$-glucan content were 34.15 g/100 g (round type) and 30.07 g/100 g (Nokgak type). The GL 70% ethanol extract at $40^{\circ}C$ showed higher radical scavenging as well as carbohydrate and lipid enzyme inhibition than other conditions. At 1 mg/mL of treatment with the 70% ethanol extract at $40^{\circ}C$ of round type GL, the DPPH, ABTS, hydroxyl radical scavenging, and ${\alpha}$-glucosidase, ${\alpha}$-amylase, and pancreatic lipase inhibition activities obtained were approximately 92.85, 99.74, 58.09, 89.68, 44.68, and 67.56%, respectively.

Development of functional soy-based stew sauce including hot water extract of Cornus officinalis S. et Z (산수유 열수추출물을 첨가한 찜류용 기능성 간장소스 개발)

  • Kim, Jun-Ho;Oh, Hae-Sook
    • Journal of the Korean Society of Food Culture
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    • v.21 no.5
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    • pp.550-558
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    • 2006
  • We developed a kind of soy-based sauce for stews using three recipes and sensory evaluation processes, and examined the biological activities such as antioxidative, fibrinolytic and ${\alpha}$-glucosidase inhibitory activities of hot water extract of Cornus officinalis S. et Z in order to use as functional ingredient. The developed stew was composed of soy sauce $1\frac{1}{2}$ Tbsp, sugar $1\frac{1}{4}$ Tbsp, garlic, small green onion, powdered sesame and sesame seed oil $\frac{1}{2}$ tsp, respectively, ginger extract $\frac{1}{4}$ tsp, black pepper $\frac{1}{8}$ and water $\frac{2}{3}$C per 300 g of main ingredients(pork rib, chicken and dried pollack). It was evaluated more highly acceptable than the original recipes by sensory test. Replacement of water in the recipe of the developed stew sauce with hot water extract of Cornus officinalis S. et Z did' nt affect the sensory quality. From the above results. we concluded that hot water extract of Cornus officinalis S. et Z might be a excellent source to provide health functionality.

Physiological activity of methanol extract and fractions from Citrus grandis Osbeck pericarp (당유자 과피 메탄올 추출물 및 분획물의 생리활성 검정)

  • Ko, Hyun Min;Kim, Ju-Sung
    • Journal of Plant Biotechnology
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    • v.45 no.3
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    • pp.279-286
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    • 2018
  • Citrus grandis Osbeck pericarp is used as tea, herbal medicine, etc., but is not commercialized in various ways. So, in this study, we identified potential for use of Citrus grandis Osbeck as health functional foods, cosmetics and food preservatives. Methanol extract of Citrus grandis Osbeck pericarp was fractionated with hexane, dichloromethane, ethyl acetate and butanol, to quantitatively analyze total phenol and flavonoid, and investigate antioxidative and enzyme inhibitory activities. Total phenol and flavonoid contents were highest in ethyl acetate fraction, FRAP and ORAC results also revealed highest activity in proportion to total phenol content. DPPH radical scavenging activity revealed that ethyl acetate, butanol and dichloromethane fraction manifested highest activity without significant difference. However, dichloromethane fraction revealed higher TEAC value and tyrosinase inhibitory activity than ethyl acetate fraction, and hexane fraction manifested best results with superoxide radical scavenging activity and ${\alpha}-glucosidase$ inhibitory activity. Antimicrobial activity also revealed best effect in dichloromethane and hexane fractions. So, based on the following results, use of dichloromethane fraction as material of natural functional cosmetics, ethyl acetate fraction for health functional foods, and hexane fractions for pharmaceuticals and food preservatives, would be most practical for product development.

Phenolic Constituents of Boehmeria pannosa and α-Glucosidase Inhibitory Activity (왕모시풀의 페놀성 성분 및 항당뇨 활성)

  • Ryu, Se Hwan;Kim, Seon Beom;Yeon, Sang Won;Turk, Ayman;Jo, Yang Hee;Hwang, Bang Yeon;Ahn, Mi-Jeong;Lee, Mi Kyeong
    • Korean Journal of Pharmacognosy
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    • v.50 no.4
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    • pp.239-244
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    • 2019
  • Boehmeria pannosa (Urticaceae) is a perennial herb widely distributed in Korea. In this study, investigation on the constituents of B. pannosa was conducted by chromatographic techniques and spectroscopic analysis. As a result, nine compounds including eight phenolic compounds, 3-hydroxy-1-(4-hydroxyphenyl)propan-1-one (1), β-hydroxypropiovanillone (2), C-veratroylglycol (3), 3-hydroxy-1-(4-hydroxy-3,5-dimethoxyphenyl)-propan-1-one (4) 2,3-dihydroxy-1-(4-hydroxy-3,5-dimethoxyphenyl) -1-propanone (5), p-coumaric acid (6), 4-hydroxybenzoic acid (7), vanillic acid (8) and one lignan, (-)-(7R,8S)-dihydrodehydrodiconiferyl alcohol (9) were isolated from the EtOAc-soluble fraction of B. pannosa. Among them, compounds 5, 6 and 9 inhibited α-glucosidase inhibitory activity more than 50% at the concentration of 100 μM.

Antiviral activity of methanol extract from Ephedra sinica Stapf (마황 추출물의 항바이러스 활성)

  • Lee, Doseung;Lee, Dong-Sun
    • Food Science and Preservation
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    • v.21 no.5
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    • pp.735-739
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    • 2014
  • Ephedra sinica Stapf, known as a medicinal plant, inhibited not only syncytium formation, but also trafficking of viral glycoprotein, hemagglutinin-neuramidase (HN) to the cell-surface. Trafficking of viral glycoprotein to the surface of infected-cells results in syncytium formation in Newcastle disease virus (NDV)-infected baby hamster kidney (BHK) cells. Viral glycoprotein in the infected-cell is processed within the endoplasmic reticulum during routing into surface. The processing of viral glycoprotein like a N-linked oligosaccharide trimming by ${\alpha}$-glucosidase in cell is necessary for virus infection. Methanol extracts showed inhibitory activities ($IC_{50}$ $15{\mu}g/mL$) against ${\alpha}$-glucosidase. This suggested that E. sinica extracts inhibited the cell-surface expression of NDV-HN glycoprotein without significantly affecting HN glycoprotein synthesis in NDV-infected BHK cells.

Comparison of Biological Activities of Extracts from Different Parts of Seabuckthorn (Hippophae rhamnoides L.) (비타민나무(Seabuckthorn, Hippophae rhamnoides L.) 부위별 추출물의 생리활성 비교)

  • Park, Yu-Hwa;Lim, Sang-Hyun;Ham, Hun-Ju;Jeong, Haet-Nim;Lee, Kwang-Jae;Kim, Kyung-Hee;Kim, Song-Mun
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.39 no.7
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    • pp.975-979
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    • 2010
  • Biological activities of different parts (stems, leaves, roots, fruits) and solvents (water, ethanol) of seabuckthorn (Hippophae rhamnoides L.) grown in Korea were tested as follows. In the experiment of inhibiting $\alpha$-glucosidase activity, ethanol extract of Hippophae rhamnoides L. stem showed the highest inhibitory activity by 93% and the next highest was the ethanol extract of its leaf by 88.7%. In the case of these two extracts, the effect of inhibiting $\alpha$-glucosidase activity was extraordinarily great when comparing with control group, acarbose. In the experiment of inhibiting $\alpha$-amylase activity, water extract of leaf showed the highest result by 54.7%, among all extracts. Regarding anticancer effect for HT-29 cell and DU-145 cell, water extract of root showed 47.1% and 32.3% activities, respectively. The experiment on antibacterial activity showed that the ethanol extract from the leaf inhibitory activity of Clostridium butyricum, Proteus mirabilis, and Shigella flexneri which are the several food borne pathogenic strains. In future research, materials for biological activity appear isolated and purified and research should continue.

Change of Biological Activity of Melon (Cucumis melo L.) according to Frozen Storage Period (냉동저장기간에 따른 멜론(Cucumis melo L.)의 생리활성 변화)

  • Cho, Jun-Gu;Youn, Sun-Joo;Lee, Eun-Tag;Kim, Tae-Wan;Kwoen, Dae-Jun
    • Journal of Applied Biological Chemistry
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    • v.52 no.4
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    • pp.200-204
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    • 2009
  • The change of biological activities of melon were investigated during frozen storage. The total phenolic concentrations in melon juice and water extract were 296.25 and $433.25\;{\mu}g/mL$, respectively. The total flavonoid contents in melon juice and water extract were 20.83 and $53.58\;{\mu}g/mL$, respectively. Antioxidant activities of melon juice and water extract were determined. The DPPH of water extract of melon (85.84%) was higher than the melon juice (60.58%). ABTS of melon juice and water extract were 94.50 and 99.30%, respectively. SOD-like activity and xanthine oxidase inhibitory activity of melon of water extracts were higher than those of melon juice. $\alpha$-Glucosidase inhibitory activity of melon juice and water extract were 22.42 and 23.43%, respectively. The changes in the antioxidant activity of melon was insignificant until 6 months of frozen storage. Therefore, it was expected that frozen storage of melon was useful preservation expedient for consistent supply of raw materials.

Inhibitory Activities of Digestive Enzymes and Antioxidant Activities of Fermented Beverages Using Momordica charantia L. (여주를 첨가한 발효음료의 소화효소 억제와 항산화 활성)

  • Park, Suin;Yeo, Seoungsoon;Lee, Youngseung;Jeong, Yoonhwa;Kim, Misook
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.46 no.11
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    • pp.1308-1315
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    • 2017
  • This study was conducted to develop Momordica charantia L. juice fermented by four Lactobacillus species such as Lactobacillus paracasei (LPA), Lactobacillus plantarum (LPL), Lactobacillus rhamnosus (LRH), and Lactobacillus reuteri (LRE) as well as to investigate their inhibitory effects against digestive enzymes and antioxidant activities. Fermentation was performed at $37^{\circ}C$ without nutrient supplementation for 72 h. The pH and total lactic acid contents were within the ranges of 3.75~3.96 and 5.21~10.04% in fermented juices, respectively. The type of starter culture and fermentation time induced changes in flavonoid contents more than total phenolic contents. All juices fermented for 48 h strongly inhibited ${\alpha}$-glucosidase activity with the percentage of inhibition ranging of 91.24~95.05%. Antioxidant activities of all juices mostly increased after 48 h of fermentation. Our results suggest that fermented juice possesses inhibitory activity against digestive enzymes and antioxidant activity, and they can be used as health functional beverages.

Inhibitory Effect of Endarachne binghamiae Extract on Melanin Synthesis (미역쇠(Endarachne binghamiae)추출물의 멜라닌 생성 억제 효과)

  • Jeon, Youngsic;Jung, Yujung;Youm, Jong-Kyung;Kim, Yong Kee;Kim, Su-Nam
    • Korean Journal of Plant Resources
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    • v.26 no.5
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    • pp.526-532
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    • 2013
  • In this study, we investigated the effect of 88 marine algae extracts on melanin synthesis to develop new whitening agents. Among varieties of marine algae tested, the ethyl acetate extracts from Endarachne binghamiae (EB), Scytosiphon lomentaria, Sargassum yezoense, Ecklonia cava and Sargassum fusiforme inhibited melanin synthesis in melan-a cells. EB treatment showed the strongest inhibitory activity in melanin synthesis, compared with that of other extracts. EB-mediated inhibition of melanin synthesis appeared to be associated with inhibition of ${\alpha}$-glucosidase-dependent glycosylation of tyrosinase in melan-a cells. In addition, EB treatment did not affect mushroom tyrosinase or cell-extracted tyrosinase activity in vitro. Taken together, our findings suggest that anti-browning effect of EB on skin is mediated through regulation of ${\alpha}$-glucosidase activity and subsequent inhibition of tyrosinase activity and melanin synthesis, and further development of EB as a potential agent for skin whitening.