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Establishment of an Analytical Method for Prometryn Residues in Clam Using GC-MS (GC-MS를 이용한 바지락 중 prometryn 잔류분석법 확립)

  • Chae, Young-Sik;Cho, Yoon-Jae;Jang, Kyung-Joo;Kim, Jae-Young;Lee, Sang-Mok;Chang, Moon-Ik
    • Korean Journal of Food Science and Technology
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    • v.45 no.5
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    • pp.531-536
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    • 2013
  • We developed a simple, sensitive, and specific analytical method for prometryn using gas chromatography-mass spectrometry (GC-MS). Prometryn is a selective herbicide used for the control of annual grasses and broadleaf weeds in cotton and celery crops. On the basis of high specificity, sensitivity, and reproducibility, combined with simple analytical operation, we propose that our newly developed method is suitable for use as a Ministry of Food and Drug Safety (MFDS, Korea) official method in the routine analysis of individual pesticide residues. Further, the method is applicable in clams. The separation condition for GC-MS was optimized by using a DB-5MS capillary column ($30m{\times}0.25mm$, 0.25 ${\mu}m$) with helium as the carrier gas, at a flow rate of 0.9 mL/min. We achieved high linearity over the concentration range 0.02-0.5 mg/L (correlation coefficient, $r^2$ >0.998). Our method is specific and sensitive, and has a quantitation limit of 0.04 mg/kg. The average recovery in clams ranged from 84.0% to 98.0%. The reproducibility of measurements expressed as the coefficient of variation (CV%) ranged from 3.0% to 7.1%. Our analytical procedure showed high accuracy and acceptable sensitivity regarding the analytical requirements for prometryn in fishery products. Finally, we successfully applied our method to the determination of residue levels in fishery products, and showed that none of the analyzed samples contained detectable amounts of residues.

Comparison of Fermentation Characteristics of Italian Ryegrass (Lolium multiflorum Lam.) and Guineagrass (Panicum maximum Jacq.) during the Early Stage of Ensiling

  • Shao, Tao;Zhang, Z.X.;Shimojo, M.;Wang, T.;Masuda, Y.
    • Asian-Australasian Journal of Animal Sciences
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    • v.18 no.12
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    • pp.1727-1734
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    • 2005
  • The fermentation characteristics and mono- and di-saccharides compositions during the early stage of ensiling were studied with a temperate grass, Italian ryegrass (Lolium multiflorum Lam.) and a tropical grass, guineagrass (Panicum maximum Jacq.). The laboratory silos were kept in the room set at 25$^{\circ}C$, and then were opened on 0.5, 1, 2, 3, 5 and 7 days (14 days in Italian ryegrass) after ensiling, respectively. The Italian ryegrass silage showed a fast and large pH decrease caused by a fast and large production of lactic acid during the first 5 days of ensiling and succeeded to achieve lactic acid type fermentation; high lactic acid/acetic acid and lactic acid content at the end of ensiling (14 days), low values of pH (3.74), acetic acid, ethanol and ammonia-N/total nitrogen, none or only small amounts of Butyric acid, valeric acid and propionic acid. The guineagrass silage showed a slow decrease in pH and a slow increase in lactic acid content during the full ensiling period, causing a high final pH value, low contents of lactic acid, acetic acid, total volatile fatty acids and total organic acids. In Italian ryegrass silage, mono- and di-saccharides compositions decreased largely within the initial 0.5 day (12 h) of ensiling. Sucrose disappeared rapidly within the initial 0.5 day of ensiling, but fructose and glucose contents showed an initial rise by the activity of enzymes in plant tissues, and then decreased gradually. On the other hand, the contents of monoand di-saccharides in guineagrass showed the largest decreases due mainly to plant respiration within the initial 0.5 day of ensiling, and no initial rises in fructose and glucose contents during the early stage of ensiling because of the absence of fructans which are hydrolyzed into fructose and glucose in temperate grasses. In both silages, the rate of reduction in mono- and di-saccharides compositions within the initial 5 days of ensiling was ranked in the order of glucose>fructose>sucrose, suggesting that glucose and fructose might be more favorably utilized than sucrose by microorganisms and glucose is the first fermentation substrate. It was concluded that the silage made from Italian ryegrass with high moisture content had a good fermentation quality owing to the dominance of lactic acid bacteria and active lactic acid fermentation during the initial stage of ensiling. These results can be explained by rapid plant sap liberation and the high activity of plant enzyme hydrolyzed fructans into fructose and glucose within the initial 2 days of ensiling, which stimulate the homofermentative lactic acid bacteria growth. In ensiling a temperate grass, the physical characteristics may ensure the rapid onset of fermentation phase, which results from the smaller losses of water-soluble carbohydrates during the initial stage of ensiling and providing sufficient water-soluble carbohydrates for lactic acid bacteria. The silage made from guineagrass with intermediate dry matter and high initial mono- and di-saccharides content was stable silage. This could be explained by the higher incorporation of air during the very early stage of ensiling and the restriction of cell breakdown and juice release due to the properties of a tropical grass with coarse porosity and stemmy structures. These physical characteristics delayed the onset of lactic acid bacteria fermentation phase by extending the phases of respiration and aerobic microorganisms activity, causing the higher loss of water-soluble carbohydrates and the shortage of lactic acid bacteria fermentation substrates.

Effects of Addition Level and Chemical Type of Propionate Precursors in Dicarboxylic Acid Pathway on Fermentation Characteristics and Methane Production by Rumen Microbes In vitro

  • Li, X.Z.;Yan, C.G.;Choi, S.H.;Long, R.J.;Jin, G.L.;Song, Man K.
    • Asian-Australasian Journal of Animal Sciences
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    • v.22 no.1
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    • pp.82-89
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    • 2009
  • Two in vitro experiments were conducted to examine the effects of propionate precursors in the dicarboxylic acid pathway on ruminal fermentatation characteristics, $CH_4$ production and degradation of feed by rumen microbes. Fumarate or malate as sodium salts (Exp. 1) or acid type (Exp. 2) were added to the culture solution (150 ml, 50% strained rumen fluid and 50% artificial saliva) to achieve final concentrations of 0, 8, 16 and 24 mM, and incubated anaerobically for 0, 1, 3, 6, 9 and 12 h at $39^{\circ}C$. For both experiments, two grams of feed consisting of 70% concentrate and 30% ground alfalfa (DM basis) were prepared in a nylon bag, and were placed in a bottle containing the culture solution. Addition of fumarate or malate in both sodium salt and acid form increased (p<0.0001) pH of culture solution at 3, 6, 9 and 12 h incubations. The pH (p<0.0001) and total volatile fatty acids (VFA, p<0.05) were enhanced by these precursors as sodium salt at 3, 6 and 9 h incubations, and pH (p<0.001) and total VFA (p<0.01) from fumarate or malate in acid form were enhanced at a late stage of fermentation (9 h and 12 h) as the addition level increased. pH was higher (p<0.001) for fumarate than for malate as sodium salt at 3 h and 6 h incubations. Propionate ($C_3$) proportion was increased (p<0.0001) but those of $C_2$ (p<0.05) and $C_4$ (p<0.01 - p<0.001) were reduced by the addition of sodium salt precursors from 3 h to 12 incubation times while both precursors in acid form enhanced (p<0.011 - p<0.0001) proportion of $C_3$ from 6h but reduced (p<0.018 - p<0.0005) $C_4$ proportion at incubation times of 1, 3, 9 and 12 h. Proportion of $C_3$ was increased (p<0.05 - p<0.0001) at all incubation times by both precursors as sodium salt while that of $C_3$ was increased (p<0.001) from 6h but $C_4$ proportion was decreased by both precursors in acid form as the addition level increased. Proportion of $C_3$ was higher (p<0.01 - p<0.001) for fumarate than malate as sodium salt from 6 h incubation but was higher for malate than fumarate in acid form at 9 h (p<0.05) and 12 h (p<0.01) incubation times. Increased levels (16 and 24 mM) of fumarate or malate as sodium salt (p<0.017) and both precursors in acid form (p<0.028) increased the total gas production, but no differences were found between precursors in both chemical types. Propionate precursors in both chemical types clearly reduced (p<0.0001 - p<0.0002) $CH_4$ production, and the reduction (p<0.001 - p<0.0001) was dose dependent as the addition level of precursors increased. The $CH_4$ generated was smaller (p<0.01 - p<0.0001) for fumarate than for malate in both chemical types. Addition of fumarate or malate as sodium type reduced (p<0.004) dry matter degradation while both precursors in both chemical types slightly increased neutral detergent fiber degradability of feed in the nylon bag.

Policies for Improving Thermal Environment Using Vulnerability Assessment - A Case Study of Daegu, Korea - (열취약성 평가를 통한 열환경 개선 정책 제시 - 대구광역시를 사례로 -)

  • KIM, Kwon;EUM, Jeong-Hee
    • Journal of the Korean Association of Geographic Information Studies
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    • v.21 no.2
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    • pp.1-23
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    • 2018
  • This study aims to propose a way for evaluating thermal environment vulnerability associated with policy to improve thermal environment. For this purpose, a variety of indices concerning thermal vulnerability assessment and adaptation policies for climate change applied to 17 Korean cities were reviewed and examined. Finally, 15 indices associated with policies for improving thermal environment were selected. The selected indices for thermal vulnerability assessment were applied to Daegu Metropolitan City of South Korea as a case study. As results, 15 vulnerability maps based on the standardized indices were established, and a comprehensive map with four grades of thermal vulnerability were established for Daegu Metropolitan City. As results, the area with the highest rated area in the first-grade(most vulnerable to heat) was Dong-gu, followed by Dalseo-gu and Buk-gu, and the highest area ratio of the first-grade regions was Ansim-1-dong in Dong-gu. Based on the standardized indices, the causes of the thermal environment vulnerability of Ansim-1-dong were accounted for the number of basic livelihood security recipients, the number of cardiovascular disease deaths, heat index, and Earth's surface temperature. To improve the thermal environment vulnerability of Ansim-1-dong, active policy implementation is required in expansion and maintenance of heat wave shelters, establishment of database for the population with diseases susceptible to high temperature environments, expansion of shade areas and so on. This study shows the applicability of the vulnerability assessment method linked with the policies and is expected to contribute to the strategic and effective establishment of thermal environment policies in urban master district plans.

Changes of lateral cephalometric values according to the rotation of head (두부회전에 따른 측모두부방사선 계측치의 변화)

  • Kim, Kwang-Soo;Hwang, Mee-Sun;Choi, Eui-Hwan;Kim, Kwang-Won;Yoon, Young-Jooh
    • The korean journal of orthodontics
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    • v.30 no.1 s.78
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    • pp.53-66
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    • 2000
  • This study was performed to find out the effect of projection errors on cephalometric linear and angular measurements according to head rotation during taking lateral cephalometric radiographs. Seventeen skulls with permanent dentition and no gross asymmetry were obtained from the Department of Anatomy, Medical School, Chosun University. Total 527 x-ray films were taken with $1^{\circ}$ interval from the reference position($0^{\circ}$) to ${\pm}15^{\circ}$ around the vertical axis (Z axis) which is perpendicular to the midpoint of the line connecting the center of two ear rods in submento-vertex direction. Statistical analysis was performed by paired t-test if there were statistically significant differences between the mean of the reference position($0^{\circ}$) and that of each rotation angle. The following results were obtained. 1. The projection errors of angular measurements were smaller than those of linear measurements. 2. The projection errors of angular measurements including midline landmarks were smaller than those including bilateral landmarks. 3. The horizontal linear measurements were gradually decreased when the stroll was rotated toward the film, but slightly increased and then decreased when the skull was rotated toward the focal spot. However, the changes were smaller in focal direction. 4. The projection errors of horizontal linear measurements were larger than those of vertical linear measurements. 5. The projection errors of vertical linear measurements were increased with increased distance from the rotation axis to vertical measurements. It is concluded that the use of angular measurements rather than linear measurements is recommended to minimize the projection errors.

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A proposal of soft tissue landmarks for craniofacial analysis using three-dimensional laser scan imaging (3차원 레이저 스캔을 이용한 안면 연조직 분석을 위한 계측점의 제안)

  • Baik, Hyoung-Seon;Lee, Hwa-Jin;Jeon, Jai-Min
    • The korean journal of orthodontics
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    • v.36 no.1 s.114
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    • pp.1-13
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    • 2006
  • Three-dimensional (3-D) laser scans can provide a 3-D image of the face and it is efficient in examining specific structures of the craniofacial soft tissues. Due to the increasing concerns with the soft tissues and expansion of the treatment range, a need for 3-D soft tissue analysis has become urgent. Therefore, the purpose of this study was to evaluate the scanning error of the Vivid 900 (Minolta, Tokyo, Japan) 3-D laser scanner and Rapidform program (Inus Technology Inc., Seoul, Korea) and to evaluate the mean error and the magnification percentage of the image obtained from 3-D laser scans. In addition, soft tissue landmarks that are easy to designate and reproduce in 3-D images of normal, Class II and Class III malocclusion patients were obtained. The conclusions are as follows; scanning errors of the Vivid 900 3-D laser scanner using a manikin were 0.16 mm in the X axis, 0.15 mm in the Y axis, and 0.15 mm in the Z axis. In the comparison of actual measurements from the manikin and the 3-D image obtained from the Rapidform program, the mean error was 0.37 mm and the magnification was 0.66%. Except for the right soft tissue gonion from the 3-D image, errors of all soft tissue landmarks were within 2.0 mm. Glabella, soft tissue nasion, endocanthion, exocanthion, pronasale, subnasale, nasal alare, upper lip point, cheilion, lower lip point, soft tissue B point, soft tissue pogonion, soft tissue menton and preaurale had especially small errors. Therefore, the Rapidform program can be considered a clinically efficient tool to produce and measure 3-D images. The soft tissue landmarks proposed above are mostly anatomically important points which are also easily reproducible. These landmarks can be beneficial in 3-D diagnosis and analysis.

Factors Affecting Complete Fetal Loss Following Multifetal Pregnancy Reduction (다태임신 감수술 (Multifetal Pregnancy Reduction) 후 완전태아손실에 영향을 미치는 인자)

  • Kim, Hye-Ok;Kim, Mun-Young;Song, Hyun-Jeong;Park, Chan-Woo;Hur, Girl;Kim, Jin-Yeong;Yang, Kwang-Mun;You, Keun-Jae;Song, In-Ok;Jun, Jong-Young;Koong, Mi-Kyoung;Kang, Inn-Soo
    • Clinical and Experimental Reproductive Medicine
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    • v.30 no.1
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    • pp.39-45
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    • 2003
  • Objective : To identify the factors affecting the complete fetal loss following multifetal pregnancy reduction (MFPR). Design: Retrospective clinical study. Methods : A total of 256 consecutive treatments of MFPR in IVF-ET cycles performed between 1992 through 2000 in Samsung Cheil hospital were analyzed. MFPR was done around 8 weeks of gestation by transvaginal ultrasono-guided aspiration in multiple pregnancies and reduced to singleton or twins. Stepwise logistic regression was performed to identify the factors affecting the final outcome of pregnancy after MFPR. Dependent variable was complete fetal loss and the independent variables were maternal age, paternal age, initial number of gestational sac (iGSNO), initial number of fetal heart beat, the number of remaining live fetus after MFPR, and chorionicity. Results: The total survival rate was 87.9%, and total fetal loss rate after MFPR was 12.1%. Total fetal loss occurred within four weeks from MFPR procedure was 1.95%. Total loss occurred after four weeks of procedure and before 24 gestational weeks was 8.2%. Seventy nine percent (202/256) of pregnancies delivered after 34 weeks of gestation. The survival rate of pregnancies reduced to singleton was significantly higher than that of pregnancies reduced to twins (93.5% vs. 86.7%, p<0.05). The mean ($\pm$SEM) gestational age at delivery was $36.2{\pm}1.0$ and $34.1{\pm}0.5$ weeks for pregnancies reduced to singletons and twins, respectively (p=0.065). Logistic regression analysis revealed that the maternal age, the number of initial gestational sac (iGSNO), and the number of remaining live fetus after MFPR significantly affected the rate of total fetal loss (Z = 0.174'age + 0.596'iGSNO + 1.324'remaining fetuses -12.07), (p<0.05). Conclusions: MFPR seems to be a relatively safe and efficient method to improve the obstetric outcome in high order multiple pregnancy. Because the maternal age, the number of initial gestational sac and the remaining live fetuses after MFPR affect the total fetal loss rate, restriction of the number of transferred embryos according to the age and MFPR to singleton fetus could be considered for the better obstetric outcome in IVF pregnancy.

Growth and Physiological Characteristics of Pinus densiflora Seedlings in Response to Open-field Experimental Warming using the Infrared Lamp (적외선등을 이용한 실외 실험적 온난화 처리가 소나무 묘목의 생장과 생리적 특성에 미치는 영향)

  • Lee, Sun Jeoung;Han, Saerom;Yoon, Tae Kyung;Han, Seung Hyun;Jung, Yejee;Yun, Soon Jin;Son, Yowhan
    • Journal of Korean Society of Forest Science
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    • v.102 no.4
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    • pp.522-529
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    • 2013
  • Climate change will affect the physiological traits and growth of forest trees. This study was conducted to investigate the effects of an experimental warming on growth and physiological characteristics of Pinus densiflora S. et Z. seedlings. One-year-old P. densiflora seedlings were planted in control and warmed plots in April 2010. The air temperature of warmed plots was increased by $3^{\circ}C$ using infrared lamps from November 2010. We measured shoot height, root collar diameter, above and below ground biomass, chlorophyll contents and leaf nitrogen concentration from March 2011 to March 2013. Seedling height and root collar diameter showed no significant difference between warmed and control plots except for root collar diameter measured in June 2012. Seedling leaf biomass was lower in the warmed ($23.94{\pm}2.10g$) than in the control ($26.08{\pm}1.72g$) plots in 2013. Shoot to root ratio (S/R ratio) was lower in the warmed ($1.09{\pm}0.07$) than in the control ($1.31{\pm}0.10$) plots in 2013. Leaf nitrogen concentrations and chlorophyll contents were not significantly different between warmed and control plots except for leaf nitrogen concentration in 2011. Leaf C/N ratio was increased in 2012 under the warming treatment. Low growth and S/R ratio in warmed plots might be related to the higher temperature and water stress. In the future, the below-ground carbon allocation of P. densiflora might be increased by global warming due to temperature and water stress.

Development of Compound Laminated Wood and Analysis of Bending Processing Properties with Major Softwoods Grown in Korea (I) - Solid wood-bending of Pinus densiflora, Pinus rigida and Larix leptolepis by steaming - (국산(國産) 주요(主要) 침엽수종(針葉樹種)의 복합집성재개발(複合集成材開發) 및 휨가공성(加工性) 분석(分析)(I) - 소나무, 리기다소나무 및 낙엽송의 증자(蒸煮)에 의한 소재(素材)휨가공(加工) -)

  • So, Won-Tek;Lim, Kie-Pyo
    • Journal of the Korean Wood Science and Technology
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    • v.17 no.3
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    • pp.8-19
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    • 1989
  • The proportion of imported wood has been now over 80% of raw materials needed in the wood industry, and therefore, many of studies on the substitution of domestic wood for imported wood and on the increasing the utilization rate of domestic species have been attempted and considered as very important projects to solve. But these trials were practically restricted by several disadvantages of domestic species. such as a small diameter, variety of species, and irregularity of wood physical or chemical characteristics, etc. From a this point of view, this study was carried out to investigate solid wood bending properties of sample trees and then to develop their end uses. The species of sample trees were Pinus densiflora S. et Z., Pinus rigida Mill., and Larix leptolepis Gord. which have large growing stocks but Pinus rigida and Larix leptolepis have not been used well because of their poor qualities. The results obtained were as follows: 1. The optimum conditions for solid wood bending processing of Pinus densiflora, Pinus rigida, and Larix leptolepis are showed in Table 6. 2. The minimum solid-bending radii of Pinus densifjora, Pinus rigida, and Larix ieptolepls were 260mm, 240mm, and 300mm, respectively in steaming treatment.

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The Effects of Smartphone Addiction on Nursing Students' Adaptation to College Life : Focusing on Mediation Effect of Social Support (간호대학생의 스마트폰 중독이 대학생활 적응에 미치는 영향 : 사회적지지의 매개효과를 중심으로)

  • Park, Ju Young;Park, Seong En;Park, Min Jo
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.19 no.1
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    • pp.218-229
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    • 2018
  • The purpose of this study was to provide basic data on smartphone addiction intervention as well as college adaptation education and policy development by confirming the mediating effect of social support in relation to the effect of smartphone addiction on college life adaptation in nursing college students. The subjects of this study were 173 students who agreed to participate in this study among 4-year nursing college students in D city. The collected data were analyzed by descriptive statistics, One-way ANOVA, Pearson's Correlation, Hierarchical Multiple Regression, and Sobel test using the SPSS 23.0 statistical program. In the results, the number of subjects showing smartphone addiction was 47 in the high risk group, 29 in the potential risk group, and 97 in the general group, and the average level of adaptation to college life in the smartphone addiction group was statistically lower as the level of addiction increased. The average social support level of the smartphone addiction group was not significant. There was a negative correlation between students' adaptation to college life and smartphone addiction (r=-.34, p<.001) and a positive correlation with social support (r=.38, p<.001). Social support showed a significant negative correlation with smartphone addiction (r=-.17, p=.023). Moreover, social support was partially mediated. Based on the results of the study, it was found that social support and related college policies are needed to improve students' adaptation to college life.