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http://dx.doi.org/10.9721/KJFST.2013.45.5.531

Establishment of an Analytical Method for Prometryn Residues in Clam Using GC-MS  

Chae, Young-Sik (Pesticide and Veterinary Drug Residues Division, Department of Food Safety Evaluation, National Institute of Food and Drug Safety Evaluation, Ministry of Food and Drug Safety)
Cho, Yoon-Jae (Pesticide and Veterinary Drug Residues Division, Department of Food Safety Evaluation, National Institute of Food and Drug Safety Evaluation, Ministry of Food and Drug Safety)
Jang, Kyung-Joo (Pesticide and Veterinary Drug Residues Division, Department of Food Safety Evaluation, National Institute of Food and Drug Safety Evaluation, Ministry of Food and Drug Safety)
Kim, Jae-Young (Pesticide and Veterinary Drug Residues Division, Department of Food Safety Evaluation, National Institute of Food and Drug Safety Evaluation, Ministry of Food and Drug Safety)
Lee, Sang-Mok (Pesticide and Veterinary Drug Residues Division, Department of Food Safety Evaluation, National Institute of Food and Drug Safety Evaluation, Ministry of Food and Drug Safety)
Chang, Moon-Ik (Pesticide and Veterinary Drug Residues Division, Department of Food Safety Evaluation, National Institute of Food and Drug Safety Evaluation, Ministry of Food and Drug Safety)
Publication Information
Korean Journal of Food Science and Technology / v.45, no.5, 2013 , pp. 531-536 More about this Journal
Abstract
We developed a simple, sensitive, and specific analytical method for prometryn using gas chromatography-mass spectrometry (GC-MS). Prometryn is a selective herbicide used for the control of annual grasses and broadleaf weeds in cotton and celery crops. On the basis of high specificity, sensitivity, and reproducibility, combined with simple analytical operation, we propose that our newly developed method is suitable for use as a Ministry of Food and Drug Safety (MFDS, Korea) official method in the routine analysis of individual pesticide residues. Further, the method is applicable in clams. The separation condition for GC-MS was optimized by using a DB-5MS capillary column ($30m{\times}0.25mm$, 0.25 ${\mu}m$) with helium as the carrier gas, at a flow rate of 0.9 mL/min. We achieved high linearity over the concentration range 0.02-0.5 mg/L (correlation coefficient, $r^2$ >0.998). Our method is specific and sensitive, and has a quantitation limit of 0.04 mg/kg. The average recovery in clams ranged from 84.0% to 98.0%. The reproducibility of measurements expressed as the coefficient of variation (CV%) ranged from 3.0% to 7.1%. Our analytical procedure showed high accuracy and acceptable sensitivity regarding the analytical requirements for prometryn in fishery products. Finally, we successfully applied our method to the determination of residue levels in fishery products, and showed that none of the analyzed samples contained detectable amounts of residues.
Keywords
prometryn; GC-MS; clam; herbicide; analytical method;
Citations & Related Records
Times Cited By KSCI : 1  (Citation Analysis)
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1 Kim JH. Pesticide and its importance. Vol. 2, pp 48-52. In: Safe Food Forum. The Korean Society of Food Hygiene and Safety, Seoul, Korea (2007)
2 Lang DH, Rettie AE, Bocker RH. Identification of enzymes involved in the metabolism of atrazine, terbuthylazine, ametryne, and terbutryne in human liver microsomes. Chem. Res. Toxicol. 10: 1037-1044 (1997)   DOI   ScienceOn
3 Brvar M, Okrajsek R, Kosmina P, Staric F, Keps R, Kozelj G, Bunc M. Metabolic acidosis in prometryn (triazine herbicide) self-poisoning. Clin. Toxicol. 46: 270-273 (2008)   DOI   ScienceOn
4 Navarro S, Vela N, Gimenez MJ, Navarro G. Persistence of four s-triazine herbicides in river, sea and groundwater samples exposed to sunlight and darkness under laboratory conditions. Sci. Total Environ. 329: 87-97 (2004)   DOI   ScienceOn
5 Sabik H, Jeannot R, Rondeau B. Multiresidue methods using solid-phase extraction techniques for monitoring priority pesticides, including triazines and degradation products, in ground and surface waters. J. Chromatogr. A 885: 217-236 (2000)   DOI   ScienceOn
6 Dean JR, Wade G, Barnabas IJ. Determination of triazine herbicides in environmental samples. J. Chromatogr. A 733: 295-335 (1996)   DOI   ScienceOn
7 Sanchez-Ortega A, Unceta N, Gomez-Caballero A, Sampedro MC, Akesolo U, Goicolea MA, Barrio RJ. Sensitive determination of trianines in underground waters using stir bar sorptive extraction directly coupled to automated thermal desorption and gas chromatography-mass spectrometry. Anal. Chim. Acta 641: 110-116 (2009)   DOI   ScienceOn
8 EPA. Reregistration eligibility decision (RED) prometryn. U.S. Environmental Protection Agency, Washington, DC, USA (1996)
9 Chunhong Z, Yun L, Wei Y, Xiaogang C, Zhengxing C. Simultaneous determination of residues of triazine herbicides in wheat by ultra performance LCTM. Mod. Sci. Instrum. 4: 70-73 (2007)
10 MFDS. Food code. Ministry of Food and Drug Safety, Cheongwon, Korea. pp. appendix.4.52. (2012)
11 MFDS. Food code. Ministry of Food and Drug Safety, Cheongwon, Korea. pp. 10.4.478-483 (2012)
12 MFDS. Food code. Ministry of Food and Drug Safety, Cheongwon, Korea. pp. 10.4.2-10 (2012)
13 Oh JH, Kwon CH, Jeon JS, Choi DM. Management of veterinary drug residues in food. Korean J. Environ. Agric. 28: 310-325 (2009)   과학기술학회마을   DOI   ScienceOn
14 Codex. Codex Guidelines for the Establishment of a Regulatory Programme for Control of Veterinary Drug Residues in Foods, CAC/GL 16. Codex Alimentarius Commission, Roma, Italy (1993)
15 AOAC. Official Method of Analysis of AOAC Intl. 18th ed. Method 2007.01. Association of Official Analytical Communities, Arlington, VA, USA (2010)
16 U.S. FDA. Pesticide analytical manual, Vol 1: Multi-residue method, 3rd ed. U.S. Food and Drug Administration, Silverspring, MD, USA (1999)
17 Lee YD. Manual of analytical method for residue pesticide on Korean food standards codex. Ministry of Food and Drug Safety, Cheongwon, Korea. pp. 11-25 (2012)