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Ameliorating effect of the ethyl acetate fraction of Pteridium aquilinum on glucose-induced neuronal apoptosis (포도당으로 유도된 신경세포 손상에 대한 고사리 아세트산에틸 분획물의 개선 효과)

  • Park, Seon Kyeong;Guo, Tian Jiao;Kim, Jong Min;Kang, Jin Yong;Park, Sang Hyun;Kang, Jeong Eun;Kwon, Bong Seok;Lee, Chang Jun;Lee, Uk;Heo, Ho Jin
    • Korean Journal of Food Science and Technology
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    • v.49 no.4
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    • pp.430-437
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    • 2017
  • The protective effect of Pteridium aquilinum on high glucose-induced cytotoxicity was examined in vitro to investigate the relationship between diabetic condition and neuronal dysfunction. The ethyl acetate fraction of P. aquilinum (EFPA), with total phenolic content of 265.08 mg gallic acid equivalent/g, showed higher 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid)/2,2-diphenyl-1-picrylhydrazyl radical scavenging activities and lipid peroxidation inhibitory effect than any other fraction. In addition, EFPA showed a significant reduction in the inhibitory effect on ${\alpha}$-glucosidase activity ($IC_{50}$ value=$205.26{\mu}g/mL$) compared to the acarbose positive control. The anti-oxidative effect in PC12 cells, protective effects on high glucose-induced oxidative stress in neuronal cells, and neurotoxicity were measured using 2',7'-dichlorofluorescin diacetate, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazoliumbromide, and lactate dehydrogenase assays, respectively. EFPA showed conspicuous inhibitory effect on cellular reactive oxygen species production and neuronal cell apoptosis. Finally, kaempferol-3-glucoside was identified as the main phenolic compound of EFPA using high performance liquid chromatography.

Evaluation on the Purification Capacity of Pollutants in the Tidal Flat (갯벌의 오염물질 정화능력 평가)

  • YOU Sun-Jae;KIM Jong-Gu
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.32 no.4
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    • pp.409-415
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    • 1999
  • To evaluate the purification capacity of pollutants (COD, $PO_4^{3-}-p$, $NO_3^{-}-N$, Cu, Cd, Pb) in the three tidal flats, Eueunri, Gyewhado, Chunjangdae, the experiments were carried out with the batch reactor equipped with artifical tidal flats. Eueunri tidal flat was $98.8\%$ content of silt and clay and Chungjangdae was $97.84\%$ content of sand. The organic matters (I.L., $COD_{sed}$, POC) in Eueunri tidal flat were 2$\~$8 times higher than other tidal flats. The purification capacity of COD was 0.75 kg/ha/12 hr in Eueunri, 0.60 kg/ha/12 hr in Gyewhado and 0.55 kg/ha/12 hr in Chunjangdae. The mean purification capacity of COD in three tidal flats was 1.27 kg/ha/day. The calculated purification capacity of COD was 25.4 ton/day in the disappeared tidal flat areas (20,000 ha) of the Saemangeum reclamation. The purification capacity of phosphorus was 0.21 kg/ha/12 hr in Gyewhado, 0.39 kg/ha/12 hr in Eueunri and 0.22 kg/ha/12hr in Chunjangdae. The nitrate was 0.53, 0.74 and 0.43kg/ha/12hr, respectively. The purification capacity of heavy metals (Cu, Cd, Pb) were 88.9g/ha/12hr, 11.0g/ha/12hr, 1.7g/ha/12hr in Gyewhado, 89.1g/ha/12hr, 18.0g/ha/12hr, 2.6g/ha/12hr in Eueunri and 55.3g/ha/12hr, 18.0g/ha/12hr, 2.1g/ha/12hr in Chunjangdae, respectively. Accordingly, the purification capacity of pollutants in Eueunri tidal flat with high contents of organic matter were higher than other tidal flats. So, the purification capacity of pollutants were affected by physical and/or chemical characteristics of tidal flats.

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Effects of specimens dimension on the flexural properties and testing reliability of dental composite resin (치과용 복합레진의 굽힘 특성과 시험 신뢰도에 미치는 시편 크기의 영향)

  • Im, Yong-Woon;Hwang, Seong-sig;Kim, Sa-hak;Lee, Hae-Hyoung
    • Korean Journal of Dental Materials
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    • v.44 no.3
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    • pp.273-280
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    • 2017
  • The aim of the present study was to investigate the effects of specimen dimension on the flexural properties and testing reliability of dental composite resin. The composite resin was prepared experimentally by mixing a resin matrix with silanated micrometer glass filler at 50 vol%. Flexural specimens with various dimension in specimen's width were fabricated by light curing using a split metal mold; $25{\times}2{\times}2mm$, $25{\times}2{\times}4mm$, $25{\times}2{\times}6mm$ in length ${\times}$ height ${\times}$ width. The flexural strength and modulus were determined according to ISO 4049 test protocol at a span length of 20 mm (normal-flexural strength; NFS). Another flexural test was conducted using mini-sized specimens ($12{\times}2{\times}2mm$, $12{\times}2{\times}4mm$, $12{\times}2{\times}6mm$) from the broken specimens at a span length of 10 mm (mini-flexural strength; MFS). Data were analyzed with ANOVA and Duncan's post-hoc test and the test reliability was evaluated by Weibull analysis. Results showed that there are generally no significant difference in flexural strength with the increase in the specimen width in NFS and MFS tests. However, the test reliability of flexural strength based on Weibull analysis was largely changed with the variables in the dimension of width and span length. The flexural modulus of NFS was increased as the dimension of specimens width increased while there was no trend in flexural modulus of MFS test. Overall results recommend that the evaluation of flexural properties and the reliability of dental composite resins should be performed with more than one test method.

Effect of Fertilizer Component on Turfgrass Growth and Quality of Golf Putting Greens under Traffic Stress (답압하에서 질소, 인산, 칼륨 변화가 골프코스 그린 잔디의 생육과 품질에 미치는 영향)

  • Lee, Sung-Woo;Lee, Jae-Pil;Kim, Doo-Hwan
    • Asian Journal of Turfgrass Science
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    • v.21 no.2
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    • pp.137-146
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    • 2007
  • Traffic causes more and more stress and injury to grasses on golf course green in Korea due to the increased playing. This study compared the effect of fertilizer component (Nitrate, Phosphate, Potassium) on turfgrass growth and quality golf course green under traffic stress during early winter. Experiment was conducted by using different fertilizer components for 2 months(Oct. 1 to Nov. 30, 2005). Turfgrass leaf color, leaf texture, density and traffic tolerance were evaluated visually, and the root length(cm) and tiller density(tiller/$cm^2$) were measured. Creeping bentgrass(Agrostis palustris cv. 'Seaside II') fromthe nursery of Incheon Grand Golf Club was used. Results of this study are as followings: 1). Turfgrass color was the best in A6(20-15-10) and A5(15-15-10) treatments in the N study. Leaf texture was not different among treatments. Turf quality and traffic tolerance were the highest In A5 and A6. Root length was the longest(15.8cm) in A6 (20-15-10). Root length increased with nitrogen levels. 2). Turf color of A9(5-7.5-5) and A10(15-22.5-15) was darkest in the comparison of P study. Leaf texture was the best in A4(10-15-10) and A9. Turf quality was the best in A10. A7(10-0-10). 3). In general, to recover turfgrass damage on the putting greens during low temperature period, fertilizer amount need to be increased; and nitrogen is better than phosphate and potassium for that purpose.

Establishment on Fertilizer Recommendation and Soil Characteristics of Rice Paddy with Environment-friendly Cultivation (농가실천 친환경 벼재배 논의 토양특성 및 시비추천량)

  • Yang, Chang-Hyu;An, Seung-Hyun;Kim, Taek-Kyum;Kim, Sun;Baek, Nam-Hyun;Choi, Weon-Young;Lee, Jang-Hee;Jeong, Jae-Hyeok;Kim, Si-Ju
    • Korean Journal of Soil Science and Fertilizer
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    • v.44 no.3
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    • pp.347-352
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    • 2011
  • Environment-friendly agriculture (EFA) are aimed to reduce use of chemical inputs as possible the recycling of resources and the environment while preserving the local resources in the long term to ensure a constant productivity and profitability for producing safe food. This study was conducted to investigate characteristics on soil environment at paddy field of environment-friendly agriculture regions (EFARs) in Honam area. Survey point of EFARs Chungnam, Jeonbuk and Jeonnam, rice bran farming method in two districts, rice bran + snail farming method in eight districts, snail farming method in five districts and ducks farming method in three districts a total of 18 districts were selected. Annual of farming method, friendly-environment certification, amount of applied fertilizer, and history of cultivation to the farm household were surveyed. The content of available phosphate and silicate among the soil chemical properties in EFA paddy field were a little lower than optimum level, and those of agricultural methods fertilized with rice bran were a little lower than those of others. Hardness among the soil physical properties in EFA paddy field were a little lower than conventional practices, and that of agricultural methods fertilized with rice bran were a little lower than those of others. We showed fertilizer recommendation dose about soil nutritional shortages according to fertilization prescriptions index by crops.

Mulberry leaf yield and optimal amount of silkworms rearing in different mulberry cultivars for mulberry fruit production (오디 생산용 뽕나무 품종별 뽕잎 생산량 및 적정 누에 사육량)

  • Lim, Ju Rak;Moon, Hyung Cheol;Kwon, Suk Ju;Kim, Dong Wan;Kwak, Dong Ok
    • Journal of Sericultural and Entomological Science
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    • v.53 no.2
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    • pp.82-86
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    • 2015
  • This study was carried out to develop the silkworm rearing technique in unused mulberry leaves after harvesting mulberry fruit. The growth of Gwasang No. 2 and Suhyang was very good compared to control cultivar Chungil in leaves size and new branch growth, but new branch and leaves of Daeshim was similar or small to control cultivar Chungil. The number of leaves of Gwasang No. 2 and Suhyang was lower than Chungil, but weight of leaves per tree was heavyer than Chungil. Mulberry leaf yield was Gwasang No. 2 521 kg/10a, Suhyang 189 kg/10a, Daeshim 73 kg/10a, Chungil 1,095 kg/10a. Content of mineral element of all three mulberry cultivars leaf for mulberry fruit production was higher than Chungil in N, P, K, Ca etc. Feeding quantity of silkworm of Gwasang No. 2 and Suhyang was much more than Chungil. Feeding quantity was highest at Suhyang in 96 kg/box. Growth duration of silkworm larvae was not different in all four mulberry culivars but weight of silkworms (5th instar 3rd day) was heavy at Gwasang No. 2 (2.07 g/head) and Suhyang (2.11 g/head) compared to control cultivar Chungil (1.92 g/head). Mortality of silkworms was 14.6% (Gwasang No. 2), 13.3% (Suhyang), 13.9% (Daesim), 12.6% (Chungil) and than higher at elder instar stage. The production amount of silkworm (5th instar 3rd day) was 35.4 kg/box (Gwasang No. 2), 36.6 kg/box (Suhyang), 35.0 kg/box (Daeshim), 33.6 kg/ box (Chungil). Amount of possible rearing silkworms was estimated 3.4box/10 a (Gwasang No. 2), 1.3box/10a (Suhyang), 0.5box/10a (Daeshim), 8.7box/10a (Chungil).

A Study on the Improvement of Pasture Productivity in a horse grazing low productive pasture (말방목 부실초지의 목초생산성 향상 연구)

  • Kim, Young Jin;Song, Sang Taek;Hwang, Kyung Jun;Kim, Si Hyun;Park, Nam Gun
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.38 no.4
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    • pp.266-272
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    • 2018
  • In this study, we created grassland establishment(paddock No.39), grassland improvement(Paddock No.44), and tall fescue seeding(paddock No.64), and the rate botanical composition, DM yield, nutritive value were examined through 2016 to 2017. The rate of botanical composition was 81% in 2016, 75% in 2017, up to 21% higher than No.39 67%, 60%, No.44 58% and 54%. The annual average DM yield was the highest at 13,234kg/ha in the district, followed by No.39, No.44, followed by 10,636kg/ha and 10,235kg/ha, respectively. The crude protein content was the highest at No.39 12.16%, with No.44 and No.64 showing 10.7%, respectively. NDF content (average for two years) was 55.90% in No.44 and No.39, slightly lower than 58.42% and 57.00%, respectively. The two-year ADF average content was 31.07% for the same trend as the NDF capacity in the No.44, below 31.71% and 32.65%, respectively. To sum up the results, Although there was a high level of incentive sowing plot(No.39) in nutritive value, considering the botanical composition and productivity of the pasture, it is also deemed desirable to have a tall fescue plot(No.64).

Spatio-temporal Distribution Patterns of Lotic Benthic Macroinvertebrate Communities in Namhan-River Weir Section (남한강 보 구간 유수성 저서성 대형무척추동물의 시·공간적 분포 특성)

  • Kim, Jin-Young;Lee, Seung-Hyun;Lee, Kyung-Lak;Noh, Seongyu;Shin, Yuna;Lee, Su-Woong;Lee, Jaekwan;Won, Doo-hee;Lim, Sung-ho;Kown, Yongju;Kong, Dongsoo
    • Korean Journal of Ecology and Environment
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    • v.51 no.4
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    • pp.331-344
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    • 2018
  • Lotic organisms in streams are affected by natural and anthropogenic disturbances such as increase of heavy rainfall event caused by climate change and flow regime change caused by weir constructions. Based on domestic and foreign literature, 157 Korean benthic macroinvertebrate taxa were selected species as potential lotic candidates. Three shoreline sites (total 54 samples) were surveyed consecutively before ('08~'09), during ('10~'12) and after ('13~'16) the construction of the weirs (Gangcheon, Yeoju and Ipo weir) in the Namhan-River for tracing changes of lotic communities. As a result, water flow of the Ipo-wier and water quality variables such as T-N, T-P, BOD5, etc. of the weir section revealed no significant changes. Physical habitat conditions such as the flow velocity and streambed substrate evidently changed. Particulary, flow velocity measured at sampling points along with each microhabitat drastically decreased and particle size of streambed substrate steadily decreased after weir constructions. Lotic organisms also decreased after construction, especially Hydropsychidae (insecta: Trichoptera) acutely decreased from $3,526ind.\;m^{-2}$ to $2ind.\;m^{-2}$ As a result of CCA, lotic species such as Hydropsyche valvata, Hydropsyche kozhantschikovi, Cheumatopsyche brevilineata, Cheumatopsyche KUa, Macrostemum radiatum, etc. correlated with the flow velocity, streambed substrate. Therefore, the decrease of the flow velocity and substrate size after weir construction seemed to be closely related to the decrease of the individual abundances of the lotic organisms independently of water quality. In order to evaluate the influence of the ecosystem on the flow regime change more accurately, it is necessary to study the indicator species based on the resistance or preference of the flow.

Effects of Salt Stress on Dry Matter, Glucose, Minerals Content and Composition in Potato (Solanum tuberosum L.) (염스트레스가 감자(Solanum tuberosum L.)의 건물, 환원당, 무기성분의 함량 및 조성에 미치는 영향)

  • Im, Ju Sung;Kim, Mi Ok;Hong, Me Soon;Kim, Mi Suk;Cheun, Chung ki;Park, Yeong Eun;Cho, Ji Hong;Cho, Kwang Soo;Chang, Dong Chil;Choi, Jang Gyu;Lee, Jong Nam
    • Korean Journal of Environmental Agriculture
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    • v.38 no.1
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    • pp.38-46
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    • 2019
  • BACKGROUND: Salinity is one of the main environmental stresses deteriorating qualities as well as yields of food crops. This study was conducted to identify the effects of salt stress on dry matter ratio, glucose content, and mineral content and composition in potatoes (Solanum tuberosum L.). METHODS AND RESULTS: Four potato varieties were grown in plastic pots (diameter 20 cm and height 25 cm) with three salinity levels (EC: 1.0, 4.0, and 8.0 dS/m) in a glasshouse. Dry matter ratio, specific gravity, starch, and glucose content in tubers harvested at 90 days after sowing were analyzed. Also, mineral contents (T-N, T-C, $P_2O_5$, $K^+$, $Ca^{2+}$, $Mg^{2+}$, $Na^+$) in stem, leaf, and tuber were investigated and statistically analyzed for analysis of variance (ANOVA). Dry matter ratio, specific gravity, and starch content in tubers were reduced in all varieties as the salt concentration increased. Glucose content tended to decrease according to the salt concentration. In ANOVA analysis of mineral contents, there were significant differences in $K^+$ and $Mg^{2+}$ of leaf and stem, in $Na^+$ of leaf and tuber, and also in $Ca^{2+}$ of leaf by the interactions of variety and salinity. In the case of $K^+/Na^+$ and $Ca^{2+}/Na^+$, the stem was more sensitively influenced by the salt treatment than the leaf or the tuber. The $K^+/Na^+$ and $Ca^{2+}/Na^+$ decreased in leaf, stem, and tuber of four varieties, as the salt concentration became higher. The decreasing level varied according to the varieties. 'Kroda' and 'Duback' maintained relatively higher $K^+/Na^+$ and $Ca^{2+}/Na^+$ than 'Atlantic' or 'Goun' under the salt stress conditions. CONCLUSION: The composition and accumulation of minerals in potato plant as well as dry matter ratio, starch, and glucose contents were significantly influenced by salt stress. The respond patterns were different depending on the varieties and it was related to the salt tolerance among varieties.

Coarse Woody Debris (CWD) Respiration Rates of Larix kaempferi and Pinus rigida: Effects of Decay Class and Physicochemical Properties of CWD (일본잎갈나무와 리기다소나무 고사목의 호흡속도: 고사목의 부후등급과 이화학적 특성의 영향)

  • Lee, Minkyu;Kwon, Boram;Kim, Sung-geun;Yoon, Tae Kyung;Son, Yowhan;Yi, Myong Jong
    • Journal of Korean Society of Forest Science
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    • v.108 no.1
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    • pp.40-49
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    • 2019
  • Coarse woody debris (CWD), which is a component of the forest ecosystem, plays a major role in forest energy flow and nutrient cycling. In particular, CWD isolates carbon for a long time and is important in terms of slowing the rate of carbon released from the forest to the atmosphere. Therefore, this study measured the physiochemical characteristics and respiration rate ($R_{CWD}$) of CWD for Larix kaempferi and Pinus rigida in temperate forests in central Korea. In summer 2018, CWD samples from decay class (DC) I to IV were collected in the 14 forest stands. $R_{CWD}$ and physiochemical characteristics were measured using a closed chamber with a portable carbon dioxide sensor in the laboratory. In both species, as CWD decomposition progressed, the density ($D_{CWD}$) of the CWD decreased while the water content ($WC_{CWD}$) increased. Furthermore, the carbon concentrations did not significantly differ by DC, whereas the nitrogen concentration significantly increased and the C/N ratio decreased. The respiration rate of L. kaempferi CWD increased significantly up to DC IV, but for P. rigida it increased to DC II and then unchanged for DC II-IV. Accordingly, except for carbon concentration, all the measured characteristics showed a significant correlation with $R_{CWD}$. Multiple linear regression showed that $WC_{CWD}$ was the most influential factor on $R_{CWD}$. $WC_{CWD}$ affects $R_{CWD}$ by increasing microbial activity and is closely related to complex environmental factors such as temperature and light conditions. Therefore, it is necessary to study their correlation and estimate the time-series pattern of CWD moisture.