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http://dx.doi.org/10.9721/KJFST.2017.49.4.430

Ameliorating effect of the ethyl acetate fraction of Pteridium aquilinum on glucose-induced neuronal apoptosis  

Park, Seon Kyeong (Division of Applied Life Science (BK21 plus), Institute of Agriculture and Life Science, Gyeongsang National University)
Guo, Tian Jiao (Division of Applied Life Science (BK21 plus), Institute of Agriculture and Life Science, Gyeongsang National University)
Kim, Jong Min (Division of Applied Life Science (BK21 plus), Institute of Agriculture and Life Science, Gyeongsang National University)
Kang, Jin Yong (Division of Applied Life Science (BK21 plus), Institute of Agriculture and Life Science, Gyeongsang National University)
Park, Sang Hyun (Division of Applied Life Science (BK21 plus), Institute of Agriculture and Life Science, Gyeongsang National University)
Kang, Jeong Eun (Division of Applied Life Science (BK21 plus), Institute of Agriculture and Life Science, Gyeongsang National University)
Kwon, Bong Seok (Division of Applied Life Science (BK21 plus), Institute of Agriculture and Life Science, Gyeongsang National University)
Lee, Chang Jun (Division of Applied Life Science (BK21 plus), Institute of Agriculture and Life Science, Gyeongsang National University)
Lee, Uk (Division of Special Purpose Tree, National Institute of Forest Science)
Heo, Ho Jin (Division of Applied Life Science (BK21 plus), Institute of Agriculture and Life Science, Gyeongsang National University)
Publication Information
Korean Journal of Food Science and Technology / v.49, no.4, 2017 , pp. 430-437 More about this Journal
Abstract
The protective effect of Pteridium aquilinum on high glucose-induced cytotoxicity was examined in vitro to investigate the relationship between diabetic condition and neuronal dysfunction. The ethyl acetate fraction of P. aquilinum (EFPA), with total phenolic content of 265.08 mg gallic acid equivalent/g, showed higher 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid)/2,2-diphenyl-1-picrylhydrazyl radical scavenging activities and lipid peroxidation inhibitory effect than any other fraction. In addition, EFPA showed a significant reduction in the inhibitory effect on ${\alpha}$-glucosidase activity ($IC_{50}$ value=$205.26{\mu}g/mL$) compared to the acarbose positive control. The anti-oxidative effect in PC12 cells, protective effects on high glucose-induced oxidative stress in neuronal cells, and neurotoxicity were measured using 2',7'-dichlorofluorescin diacetate, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazoliumbromide, and lactate dehydrogenase assays, respectively. EFPA showed conspicuous inhibitory effect on cellular reactive oxygen species production and neuronal cell apoptosis. Finally, kaempferol-3-glucoside was identified as the main phenolic compound of EFPA using high performance liquid chromatography.
Keywords
${\alpha}$-glucosidase; antioxidant; kaempferol-3-glucoside; neuronal cell protection; Pteridium aquilinum;
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