• The Plant Pathology Journal
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• v.30 no.4
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• pp.343-354
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• 2014

Rice blast disease caused by Magnaporthe oryzae is one of the most serious diseases of cultivated rice (Oryza sativa L.) in most rice-growing regions of the world. In order to investigate early response genes in rice, we utilized the transcriptome analysis approach using a 300 K tilling microarray to rice leaves infected with compatible and incompatible M. oryzae strains. Prior to the microarray experiment, total RNA was validated by measuring the differential expression of rice defense-related marker genes (chitinase 2, barwin, PBZ1, and PR-10) by RT-PCR, and phytoalexins (sakuranetin and momilactone A) with HPLC. Microarray analysis revealed that 231 genes were up-regulated (>2 fold change, p < 0.05) in the incompatible interaction compared to the compatible one. Highly expressed genes were functionally characterized into metabolic processes and oxidation-reduction categories. The oxidative stress response was induced in both early and later infection stages. Biotic stress overview from MapMan analysis revealed that the phytohormone ethylene as well as signaling molecules jasmonic acid and salicylic acid is important for defense gene regulation. WRKY and Myb transcription factors were also involved in signal transduction processes. Additionally, receptor-like kinases were more likely associated with the defense response, and their expression patterns were validated by RT-PCR. Our results suggest that candidate genes, including receptor-like protein kinases, may play a key role in disease resistance against M. oryzae attack.

• Fisheries and Aquatic Sciences
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• v.21 no.4
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• pp.10.1-10.8
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• 2018

Substitution effect of white radish' by-product (WRB) and tunic of sea squirt (TSS) for Undaria in feed on growth and carcass composition of abalone was determined. Eight-hundred forty juveniles were distributed into 12 containers. Three formulated diets were prepared in triplicate. A 15% Undaria was included in the control diet. The 15% Undaria was substituted with a same amount of WRB and TSS, referred to as the DRG and TSS diets, respectively. Finally, dry Undaria was also prepared to compare effect of the formulated diets on performance of abalone. The experimental diets were fed to abalone once a day at a satiation level with a little leftover. The feeding trial lasted for 16 weeks. Crude protein, crude lipid, and ash content of the experimental diets changed over all period of time. Higher crude protein and lipid and ash contents retained in all formulated diets compared to the Undaria were observed at 24 and 48 h after seawater immersion. Survival of abalone was not affected by the experimental diets. Weight gain and specific growth rate (SGR) of abalone fed the TSS diet was greater than all other diets. Weight gain and SGR of abalone fed the control diet was not different from those of abalone fed the WRB diet. Proximates of the soft body of abalone was affected by the diets. In conclusion, WRB and TSS are the promising feed ingredients to replace Undaria in abalone feed; especially, TSS is superior to either Undaria or WRB. The formulated diets produced improved growth performance of abalone over the single Undaria.

• Journal of the Institute of Electronics Engineers of Korea SP
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• v.46 no.6
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• pp.163-174
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• 2009

Applying the maximally polyphase decomposition and noble identity to the adaptive filter in subband structure, the conventional fullband affine projection algorithm is translated to the subband affine projection (SAP) algorithm. The Maximally polyphase decomposed SAP (MPDSAP) algorithm is a special version of the SAP algorithm, and its adaptive sub-filters have unity projection dimension. The weight updating formular of the MPDSAP is similar to that of the NLMS algorithm, so it may be more proper algorithm than other AP-type algorithms for many practical applications. This paper presents a new statistical analysis of the MPDSAP algorithm. The analytical model is derived for autoregressive (AR) inputs and the nonunity adaptive gain in the subband structure with the orthonormal analysis filters (OAF), The pre-whitening by the OAF allows the derivation of a simple-analytical model for the MPDSAP with the AR inputs and the nonunity adaptive gain.

• Journal of The Korean Association of Information Education
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• v.23 no.6
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• pp.543-550
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• 2019

In 2006, Janet Wing defined computational thinking and operated SW education as a formal curriculum in the UK in 2013. This study collected related research papers by using computational thinking, which has recently increased in importance, and analyzed it using text mining. In the first, CONCOR analysis was conducted with the keyword of computational thinking. In the second, text mining of the components of computational thinking was selected by the repr23esentative academic journals at domestic and foreign. As a result of the two-time analysis, first, abstraction, algorithm, data processing, problem decomposition, and pattern recognition were the core of the study of computational thinking component. Second, research on convergence education centered on computational thinking and science and mathematics subjects was actively conducted. Third, research on computational thinking has been expanding since 2010. Research and development of the classification and definition of computational thinking and components and applying them to education sites should be conducted steadily.

• Journal of Digital Convergence
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• v.14 no.7
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• pp.385-395
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• 2016

This research applied Aromatherapy Foot Massage on Community elders and examined its effects on their chronic pain, sleep. The study subjects were 43 female elders in the community of including 22people for the experimental group and 21 for the control group. The aromatherapy was carried out 6times for 2weeks to the experimental group, 3times a week and 10minutes per session. The components of aroma essential oil included Chamomile roman, Lavender, Marjoram sweet, and Orange sweet that were diluted 20% into 100mL of Jojoba oil which was the carrier oil. The results, the experimental group that participated in the aromatherapy improved in chronic pain, quality of sleep, and the number of urination during sleep decreased compared to the control group. The research results demonstrated that due to the aroma foot massage, chronic pain decreased, sleep improved.

• Journal of Korean Society of Water and Wastewater
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• v.33 no.2
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• pp.95-101
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• 2019

High voltage impulse(HVI) has been gained attention as an alternate technique controlling $CaCO_3$ scale formation. Investigation of key operational parameters for HVI is important, however, those had not been reported yet. In this study, the effect of temperature and applied voltage of HVI on $Ca^{2+}$ concentration was studied. As the applied voltage from 0 to 15kV and the temperature increased from 20 to $60^{\circ}C$, the $Ca^{2+}$ concentration decreased, indicating that the aqueous $Ca^{2+}$ precipitated to $CaCO_3$. The $Ca^{2+}$ concentration decreased up to 81% under the condition of 15kV and $60^{\circ}C$. Rate constant for the precipitation reaction, k was determined under different temper1ature and voltage. The reaction rate constant under the 15kV and $60^{\circ}C$ condition was evaluated to $66{\times}10^{-3}L/(mmol{\cdot}hr)$, which was 5 times greater than the k of the reaction without HVI at same temperature. The increases in k by HVI at higher temperature region(40 to $60^{\circ}C$) was much greater than at lower temperature region(20 to $40^{\circ}C$), which implies temperature is more important parameter than voltage for reducing $Ca^{2+}$ concentration at high temperature region. These results show that the HVI induction accelerates the precipitation to $CaCO_3$, particularly much faster at higher temperature.

• Korean Journal of Medicinal Crop Science
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• v.28 no.6
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• pp.435-444
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• 2020

Background: As the Positive List System (PLS) is implemented in broad application to agricultural products, attention to the correct use of pesticides is also needed in the production of mulberry leaves and fruit. In this regard, three types of pesticides against mulberry popcorn disease were applied 2 - 3 times both in the field and greenhouses to prepare safety standards. Residual pesticide analysis was conducted on mulberry fruits and leaves. Methods and results: Three pesticieds, thiophanate-methyl, thiophanate-methyl·triflumizole and fluopyram registered as PLS pesticides for mulberry popcorn disease, were sprayed in the Wanju and in Buan regions, after which residual pesticide analysis was conducted using high-performance liquid chromatography (HPLC). As three pesticieds were either undetected or below the permissible level in mulberry fruit, demonstrating that they were suitable for safe spraying. However, 5.6 mg/kg of thiophanate-methyl was detected in the greenhouse after three application, which was slightly above maximum residue limit (MRL). Furthermore the level of thiophanate-methyl·triflumizole was higher than 0.2 mg/kg ("Gwasang No. 2" variety, spraying twice) or similar to 0.09 mg/kg ("Daesim" variety, spraying thrice) the permissible level (0.1T mg/kg) as the thiophanate-methyl was detected in mulberry leaves in the greenhouse. Conclusions: The spraying frequency for controlling mulberry popcorn disease in greenhouses should be limited to two times or less, especially when mulberry leaves are treated with thiophanate-methyl·triflumizole careful consideration is required if the leaves are to be used as food materials.

• Journal of Animal Reproduction and Biotechnology
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• v.38 no.3
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• pp.131-142
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• 2023

Background: This study has mainly focused on finding pharmacological effects of ginsenosides that can reduce the unwanted side effects of the cytotoxic anticancer drugs and are highly effective on prostate cancer, colorectal cancer, liver cancer, hormone-dependent breast cancer, triple-negative breast cancer, and brain cancer (neuroblastoma). Methods: Minor and rare ginsenosides (GS) of Rh2 which have a high absorption ability and excellent pharmacological actions were treated with the 6 different types of cancer cell lines and their anticancer activities were investigated by analyzing gene expressions associated with various cancers through qPCR and other relevant methods. Results: In cancer cells exposed to Rh2, cell viability and cell migration were reduced, and apoptosis was induced. Each cancer cell was divided into three groups according to the cell proliferation response by Rh2; 1) A group in which the cell viability decreases inversely to an increase in Rh2 treatment concentration; 2) A group in which the cell viability rapidly decreases in Rh2 treatment above a certain level of concentration; 3) A group in which the cell viability was not suppressed below 20-30% even with 100 μL of Rh2, the highest concentration used in this study. Conclusions: It was shown that Rh2 has a significant effect on inhibiting the proliferation of prostate cancer cells and hormone-dependent breast cancer cells.

• Journal of the Korean Society of Fisheries and Ocean Technology
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• v.59 no.4
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• pp.336-343
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• 2023

The aging fishery training vessels from the past have mostly been decommissioned, and many universities are introducing state-of-the-art large fishery training vessels. The purpose of these training vessels is to train marine professionals and above all, safety to prevent marine accidents should be of utmost priority as many students embark on the vessel. This study estimated the impact of the hydrodynamic interaction forces acting on the model vessel (fishery training vessel) from the bank when the vessel pass near the semi-circle bank wall in various conditions through the numerical calculation, especially concerning maneuvering motions of the vessel. For estimation, variables were mainly set as the size of the semi-circle shape, the lateral distance between the bank and the model vessel, and the depth near the bank. As a result, it was estimated that, in order for the model vessel to safely pass the semi-circle bank wall at a speed of 4 knots, the water depth to the vessel draft ratio should be 1.5 or more (approximately 8 m of water depth), and the lateral distance from the semi-circle bank wall should be 0.4 times the model vessel's length (L_pp) or more (a distance of 34 m or more). Under these conditions, it was expected that the model vessel would pass without significantly being affected by the bank wall.

• IMMUNE NETWORK
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• v.21 no.6
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• pp.44.1-44.15
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• 2021

Tumor peptides associated with MHC class I molecules or their synthetic variants have attracted great attention for their potential use as vaccines to induce tumor-specific CTLs. However, the outcome of clinical trials of peptide-based tumor vaccines has been disappointing. There are various reasons for this lack of success, such as difficulties in delivering the peptides specifically to professional Ag-presenting cells, short peptide half-life in vivo, and limited peptide immunogenicity. We report here a novel peptide vaccination strategy that efficiently induces peptide-specific CTLs. Nanoparticles (NPs) were fabricated from a biodegradable polymer, poly(D,L-lactic-co-glycolic acid), attached to H-2K^b molecules, and then the natural peptide epitopes associated with the H-2K^b molecules were exchanged with a model tumor peptide, SIINFEKL (OVA_257-268). These NPs were efficiently phagocytosed by immature dendritic cells (DCs), inducing DC maturation and activation. In addition, the DCs that phagocytosed SIINFEKL-pulsed NPs potently activated SIINFEKL-H2K^b complex-specific CD8⁺ T cells via cross-presentation of SIINFEKL. In vivo studies showed that intravenous administration of SIINFEKL-pulsed NPs effectively generated SIINFEKL-specific CD8⁺ T cells in both normal and tumor-bearing mice. Furthermore, intravenous administration of SIINFEKL-pulsed NPs into EG7.OVA tumor-bearing mice almost completely inhibited the tumor growth. These results demonstrate that vaccination with polymeric NPs coated with tumor peptide-MHC-I complexes is a novel strategy for efficient induction of tumor-specific CTLs.