The Plant Pathology Journal

The Korean Society of Plant Pathology (한국식물병리학회)
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Transcriptome Analysis of Early Responsive Genes in Rice during Magnaporthe oryzae Infection

  • Wang, Yiming (Department of Plant Microbe Interaction, Max Planck Institute for Plant Breeding Research) ;
  • Kwon, Soon Jae (Department of Plant Bioscience, Life and Industry Convergence Research Institute, Pusan National University) ;
  • Wu, Jingni (Department of Plant Microbe Interaction, Max Planck Institute for Plant Breeding Research) ;
  • Choi, Jaeyoung (Department of Agricultural Biotechnology, Center for Fungal Genetic Resources and Center for Fungal Pathogenesis, Seoul National University) ;
  • Lee, Yong-Hwan (Department of Agricultural Biotechnology, Center for Fungal Genetic Resources and Center for Fungal Pathogenesis, Seoul National University) ;
  • Agrawal, Ganesh Kumar (Research Laboratory for Biotechnology and Biochemistry (RLABB)) ;
  • Tamogami, Shigeru (Laboratory of Biologically Active Compounds, Department of Biological Production, Akita Prefectural University) ;
  • Rakwal, Randeep (Research Laboratory for Biotechnology and Biochemistry (RLABB)) ;
  • Park, Sang-Ryeol (Molecular Breeding Division, National Academy of Agricultural Science, RDA) ;
  • Kim, Beom-Gi (Molecular Breeding Division, National Academy of Agricultural Science, RDA) ;
  • Jung, Ki-Hong (Graduate School of Biotechnology and Crop Biotech Institute, Kyung Hee University) ;
  • Kang, Kyu Young (Plant Molecular Biology and Biotechnology Research Center/Division of Applied Life Science (BK21 Program), Gyeongsang National University) ;
  • Kim, Sang Gon (Plant Molecular Biology and Biotechnology Research Center/Division of Applied Life Science (BK21 Program), Gyeongsang National University) ;
  • Kim, Sun Tae (Department of Plant Bioscience, Life and Industry Convergence Research Institute, Pusan National University)
  • Received : 2014.07.04
  • Accepted : 2014.07.30
  • Published : 2014.12.01
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Abstract

Rice blast disease caused by Magnaporthe oryzae is one of the most serious diseases of cultivated rice (Oryza sativa L.) in most rice-growing regions of the world. In order to investigate early response genes in rice, we utilized the transcriptome analysis approach using a 300 K tilling microarray to rice leaves infected with compatible and incompatible M. oryzae strains. Prior to the microarray experiment, total RNA was validated by measuring the differential expression of rice defense-related marker genes (chitinase 2, barwin, PBZ1, and PR-10) by RT-PCR, and phytoalexins (sakuranetin and momilactone A) with HPLC. Microarray analysis revealed that 231 genes were up-regulated (>2 fold change, p < 0.05) in the incompatible interaction compared to the compatible one. Highly expressed genes were functionally characterized into metabolic processes and oxidation-reduction categories. The oxidative stress response was induced in both early and later infection stages. Biotic stress overview from MapMan analysis revealed that the phytohormone ethylene as well as signaling molecules jasmonic acid and salicylic acid is important for defense gene regulation. WRKY and Myb transcription factors were also involved in signal transduction processes. Additionally, receptor-like kinases were more likely associated with the defense response, and their expression patterns were validated by RT-PCR. Our results suggest that candidate genes, including receptor-like protein kinases, may play a key role in disease resistance against M. oryzae attack.

Keywords

References

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