• The Korean Journal of Mycology
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• v.14 no.1
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• pp.37-41
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• 1986

The production of cellulolytic enzymes by Pleurotus sajor-caju JAFM 1017 was stimulated by folic acid and thiamine-HCl. Among the inorganic salts, optimum concentrations of $KH_2PO_4$ and $MgSO_4{\cdot}7H_2O$ were 0.2% (w/v) and 0.04% (w/v), respectively, but other inorganic salts were not effective for the production of the enzymes. The optimum culture temperature and pH for the production were $25^{\circ}C$ and 5.5 for avicelase, and $30^{\circ}C$ and 5.0 for CMCase, and $30^{\circ}C$ and 6.5 for ${\beta}-glucosidase$, respectively.

• Applied Biological Chemistry
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• v.32 no.3
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• pp.309-314
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• 1989

The cultural condition of Enterobacter agglomerans U-1, a polysaccharide producing soil bacterium, was examined. The optimal medium composition was that contains the following components per liter of distilled water; $sucrose(23.75\;g/L),\;peptone(2.06\;g/L),\;yeast\;extract(0.5\;g/L),\;KH_2PO_4(1.0\;g/L)$ and $MgSO_4{\cdot}7H_2O(1.0 g/L)$. The optimum temperature for polysaccharide production was $30^{\circ}C$, where 8.5 g/L of polysaccharide was produced. The apparent viscosity of fermentation broth after 3 days was 240 mPa.s. at $70sec^{-1}$. The product yield and specific productivity were 36% and 142.07 mg/g/h.

• Microbiology and Biotechnology Letters
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• v.21 no.5
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• pp.435-439
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• 1993

For the overproduction of glutathione, Candida sp. mutant was isolated by the treatment with U.V. light. The highest glutathione production of Candida sp. mutant was obtained after shaking culture for 48 hours in the cullture medium containing glucose 1.5%(w/v), yeast extract 4.0% (w/v), KH2PO4 0.04%(w/v), biotin 5 ng/ml, and L-cysteine 0.04%(w/v). The optimal pH and temperature for the glutathione production were pH 6.0 and 25C, respectively.

• Microbiology and Biotechnology Letters
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• v.34 no.4
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• pp.318-322
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• 2006

The intracellular invertase gene of alkalophilic and thermophilic Bacillus sp. TA-11 which was isolated from compost was cloned and expressed in E. coil HB101 using pUC19 as a vector. The invertase of the recombinant E. coli (pYC 17) was maximally produced when it was incubated at 37$^{\circ}C$ for 9 h in a SY medium containing 0.25% sucrose, 0.5% yeast extract, 0.1% each of $K_2HPO_4$ and $KH_2PO_4$, with an initial pH of 8.0. The final enzyme activity under the above condition was 47.7 U per ml of cell-free extract.

• Research in Plant Disease
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• v.11 no.1
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• pp.66-71
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• 2005

The effect of medium components such as wheat bran, rice bran, oat meal, and soybean meal as basic ingredients and KH2PO4, glucose, and molasses as additives on mass production and anti-potato common scab activ ity of a streptomycete A020645 strain as a biocontrol agent (BCA) candidate was investigated. Of basicingredients, oat meal was the best one for mass poduction and enhancement of anti-potato common scabactivity. The biomass production of the active strain was more enhanced when 0.1-0.01.% glucose or molassesas additive were added into the basic medium. These information may have important implications in applying for effective formulation of BCA.

• Journal of Mushroom
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• v.11 no.1
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• pp.15-20
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• 2013

This study was performed to obtain the scientific data an artificial culture of ear mushroom(Auricularia auricula). Liquid cultural of Auricularia auricula. mycelium was characterized with respect to temperature, pH, compositions of inorganic, carbon and nitrogen. The its optimum temperature and media pH were at $25^{\circ}C$ and pH 5.0~6.0 on the mycelial growth, respectively. The optimum inorganic compositions and concentration for mycelial growth were $MgSO_4$ 0.05%(w/v) and $KH_2PO_4$ 0.1%(w/v), respectively. The optimum carbon sources and nitrogen sources were black-sugar 2%(w/v) and soybean powder at 0.3~0.5%(w/v) with7 days optimum culture time.

• Proceedings of the Korean Society for Bio-Environment Control Conference
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• 2002.11a
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• pp.227-232
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• 2002

냉온처리전, 후 ABA의 토양 및 엽면처리는 오이묘의 생존율을 유의하게 증가시켰고 세포의 전해질 누출량도 현저하게 감소시켰으며, 수량의 감소를 경감시켰다. 또한 ABA 처리는 냉온처리후보다는 냉온처리전에, 엽면처리보다는 토양에 처리하는 것이 보다 효과적이었다. 요소(0.2%)와 KH$_2$PO$_4$의 엽면살포는 오이의 생장과 수량에 미치는 냉온피해를 경감시켰다.

• Analytical Science and Technology
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• v.25 no.6
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• pp.460-466
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• 2012

An environmentally friendly, so-called green, high performance liquid chromatography method was developed and validated for the determination of trans, trans-muconic acid (t,t-MA) in human urine as a biomarker of benzene exposure. After urinary t,t-MA was extracted and enriched using solid-phase extraction, a MF-Ph1 SG80 ($150mm{\times}2.0mm$ I.D., 5 ${\mu}m$) column with a mobile phase of 10 mM $KH_2PO_4$ containing 0.1% $H_3PO_4$ was used for isocratic separation of t,t-MA with UV detection at 259 nm. The calibration curve was constructed in the range of 0.1-5.0 mg/L with good linearity ($r^2$=0.9992). The intra-day and inter-day precision (as RSD) were 0.9-8.5% and 3.1-4.5%, respectively. The average recovery ranged from 97.5% to 101.7%. The green sample preparation and separation with no organic solvents were successfully achieved. The validated method would be suitable for the routine biological monitoring of benzene exposure in the occupational settings.

• Microbiology and Biotechnology Letters
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• v.11 no.3
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• pp.217-222
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• 1983

By the treatment of Gibberella fujkuroi I-892 with mutagen such as UV light and N-methyl-N'-nitro-N-nitrosoguanidine, a mutant G. fujkuroi G-471 was selected as the highest producer of gibberellic acid among 800 mutant strains. It showed 30% increase of production yield compared with that of the parent strain. At optimum medium composition (saccharose 1.0%, ammonium tartarate 50mM, malt extract 1.0% KH$_2$PO$_4$ 0.5%, MgSO$_4$0.5%, FeSO$_4$0.0002%, trace element sol.0.002% (v/v), the yield of submerged culture increased by 30% after 7 days culture at 24$^{\circ}C$ (253mg/$\ell$). In submerged culture, the initial pH showed much effects on the increase of gibberellic acid production. The highest yield of the production was attained with pH adjustment to 4.0 at the initial stage of fermention.

• Journal of Life Science
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• v.22 no.2
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• pp.200-208
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• 2012

The characteristics of mycelium growth of Sparassis crispa KGFS08 and KFRI746 in liquid culture were investigated. The optimum growth of the mycelium of S. crispa was observed in the KTM medium. The best carbon source was starch. In terms of nitrogen sources, tryptone affected mycelial growth in the liquid culture. The optimal culture conditions were pH 4.0-5.0 in STK medium [3% (w/v) starch, 0.3% (w/v) tryptone, 0.1% (w/v) $KH_2PO_4$, and 0.1% (w/v) folic acid].