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http://dx.doi.org/10.5352/JLS.2012.22.2.200

Optimization of Medium Composition for the Mycelial Growth of Sparassis crispa  

Kim, Jin-Woo (Gyeongsangbuk-Do Forest and Environment Research Institute)
Cheon, Woo-Jae (Gyeongsangbuk-Do Forest and Environment Research Institute)
Chai, Kyung-Hee (Gyeongsangbuk-Do Forest and Environment Research Institute)
Kim, Dong-Gwan (Institute of Biotechnology, Vitrosys Incoporation)
Son, Sung-Ho (Institute of Biotechnology, Vitrosys Incoporation)
Kim, Jong-Guk (School of Life Science and Biotechnology, Kyungpook National University)
Lim, Hee-Jae (Gyeongsangbuk-Do Forest and Environment Research Institute)
Publication Information
Journal of Life Science / v.22, no.2, 2012 , pp. 200-208 More about this Journal
Abstract
The characteristics of mycelium growth of Sparassis crispa KGFS08 and KFRI746 in liquid culture were investigated. The optimum growth of the mycelium of S. crispa was observed in the KTM medium. The best carbon source was starch. In terms of nitrogen sources, tryptone affected mycelial growth in the liquid culture. The optimal culture conditions were pH 4.0-5.0 in STK medium [3% (w/v) starch, 0.3% (w/v) tryptone, 0.1% (w/v) $KH_2PO_4$, and 0.1% (w/v) folic acid].
Keywords
Sparassis crispa; mycelial growth; liquid culture; carbon source; nitrogen source;
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