• Title/Summary/Keyword: $K^+-channel$

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Separation and growth monitoring of oyster mushroom spores using gravitational field-flow fractionation (GrFFF) (중력 장-흐름 분획법을 이용한 느타리버섯 포자의 분리 및 성장 모니터링)

  • Kim, Sun Tae;Ji, Eun Young;Lee, Jae Chan;Lee, Seungho
    • Analytical Science and Technology
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    • v.26 no.4
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    • pp.262-267
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    • 2013
  • Gravitational field-flow fractionation (GrFFF) is a separation technique that utilizes earth's gravity as the external field. GrFFF is a convenient tool for the size and/or density-based separation of micron-sized particles of various origins. In this study, GrFFF was employed for size-based separation of oyster mushroom spores. Oyster mushroom spores have smooth surface and are of cylindrical to narrow kidney-shapes with 5 to 12 im in longer dimension and 3 to 4 im in shorter dimension, as was confirmed by optical microscope (OM). GrFFF conditions were optimized for separation and characterization of spores by varying the channel flow rate from 0.5 to 1 mL/min. During the GrFFF elution of the spores, 3 fractions were collected to confirm the growth of oyster mushroom spore. The collected fractions were incubated for 30 days in water to examine the influence of the size on the growth of the spores. Results suggested that the oyster mushroom spores collected at the middle part of the GrFFF fractogram grew faster than those collected at the beginning or at the end of the fractogram.

Depositional Environments of the Continental Shelf Sediments between Geoje and Namhae Islands (거제-남해간 해저퇴적물의 퇴적환경에 관한 연구)

  • KIM Dae-Choul;SONG Si-Tae;YOO Byeong-Jo
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.20 no.2
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    • pp.166-174
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    • 1987
  • Distribution, bathymetry and textural parameters of the bottom sediment deposited between Geoje and Namhae Islands were studied to understand the depositional environment of the area. The study area is divided into three different provinces. Except for the Gwangyang Bay and Changseon Channel, mud dominates in the western part whereas sandy mud and muddy sand prevail in the southeastern part including the eastern area of the Yogji Island. The relict sediment is located in the eastern part of the area. Generally, the Holocene sediment, located in the northern part of the area, is considered to be transported and deposited by a pelagic suspension mode. Influence of strong tidal currents results in some depressions in the vicinity of Changseon Island and the eastern part of the Yogji Island, The Tsushima Warm Current is supposed to affect the southern part of the area. The two parallels sand ridges lying in the southeastern part of the area are covered with very thin Holocene mud. This seems to be caused by the winnowing effect of the current. The similarity between the two directions of the current and the ridges encourages this idea. The boundary between the Holocene and relict sediment, however, lies further south the ridges.

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A Study on the Entrance Channel of Restrictions on Passage of Oil Tankers in Yeosu-Gwangyang Port (여수·광양항 중심의 유조선통항금지해역 출입 항로에 관한 연구)

  • Kwon, Yu-Min;Lee, Hong-Hoon;Lee, Chang-Hyun
    • Journal of the Korean Society of Marine Environment & Safety
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    • v.23 no.5
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    • pp.439-446
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    • 2017
  • The Maritime Safety Act established restrictions for the passage of oil tankers, prohibiting vessesl carrying more than 1,500 kiloliters of oil or 1,500 tons of a hazardous liquid substance. Prohibited vessels that do not satisfy the restrictions are allowed to enter and depart from a nearby port from the outer sea area to minimize the time and distance the oil tanker must navigate in the prohibited area. Therefore, such regulation should not be construed as referring to inshore traffic. In this study, the traffic volume of coastal tankers that do not use the approaching channels for specific sea areas near Yeosu and Gwangyang Port was analyzed, and the cargo loads of these ships were investigated. The results of this study should be used to allow tankers to minimize the time and distance of navigation in prohibited areas. According to the survey, 16 vessels, 51.6 % of the 31 vessels using inshore traffic included in the study, were loaded with more than 1,500 tons of cargo. This is not appropriate according to the legislation for oil tanker passage. Therefore, in this study, sea routes have been proposed that connect with the approaching channels of specific sea areas, from the outer sea areas of restricted passages. Regulations have also been proposed for the entry and departure of oil tankers around Yeosu and Gwangyang Port.

A Political Proposal for the Private Brand Activation (유통업체 PB상품 활성화를 위한 정책연구)

  • Cho, Hye-Jeong;Lee, Seung-Chang;Ryu, Sung-Min
    • Journal of Distribution Research
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    • v.17 no.5
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    • pp.113-128
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    • 2012
  • The growth of market share of distributors' brands, also known as private brand, has accelerated in recent years. Sales volumes and market shares of private brands, as well as their appeal to consumers have steadily increased. Carrying private brands comes with numerous advantages, one of which is the relatively high gross margin, which can be 20 - 30% higher compared to manufacturer brands. Recently, many big discount stores are expanding private brand for higher sales volume. Thus, private brands play an important strategic role for retailers. The tendency of growing private brand will decrease sales revenue of both channel members, distributer and manufacturer. The disadvantage for manufacturer is obvious, especially for the manufactures who not only produce their own brands but also retailer brands competing against their own. There are also possible to weaken the brand awareness of manufacturer's brand. The purpose of this study is to explore the perception gap between retailers and manufacturers. we investigated to identify how consumers perceive private brand. In other to study the impact of private brands on distributors, we surveyed the actual condition of private brand and perception towards private brands among consumers, retailers and manufacturers. Based these analysis, we recommended proposal for private brand policies as follows: First, it need to correct imbalance between large retailer and manufacturer. second, we suggest "win-win growth policy", Third, by registering trademark right of national brand, manufacturers have a way of protecting their brands. Forth, manufacturers are encouraged to produce PNB(Private National Brand).

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A Study of an Association Admission Method for Public Wireless LAN Systems (고속 공중 무선랜 시스템을 위한 효율적인 연결 수락 제어방법의 연구)

  • Lee, Hyun-Woo;Yoon, Hong;Yoon, Chong-Ho
    • Journal of Advanced Navigation Technology
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    • v.7 no.2
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    • pp.149-155
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    • 2003
  • This paper generally relates to the field of wireless local area networks(WLANs), and more particularly, to AP selection and association methods for the performance of a station. In these days, IEEE 802.11 Wireless LAN network is widely deployed and used as an emerging service to connect high-speed Internet in the public wireless environment. But, if there are many users in hot spot area, they suffer a severe decrease of performance. Thus it needs an association and access control mechanism especially when it is used in the public environment. In this paper, we suggest a selection and association method using Beacon or Probe Response frames based IEEE 802.11. Station selects AP using the information of the capacity area in a Beacon or a Probe Response frame. According to the present paper, an association method for a public WLAN service, which includes a WLAN user terminal and an AP for relaying WLAN communications to and from the user terminal, includes the steps of the user terminal asking the AP's states for access to a radio channel; and the station selects and associates with the AP. According to the above-described selection in a high speed wireless Internet service based on public WLAN technologies that are currently in operations. Further, it is possible to improve the efficiency of network management.

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Effects of Amiloride on $A_{1}$ Adenosine Receptor-Adenylyl Cyclase System in Rat Adipocytes (흰쥐 지방세포에 있어서 Amiloride의 $A_{1}$ Adenosine Receptor- Adenylyl Cyclase System에 대한 작용)

  • Park, Kyung-Sun;Lee, Myung-Soon;Kim, Kyung-Hwan
    • The Korean Journal of Pharmacology
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    • v.29 no.2
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    • pp.245-252
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    • 1993
  • Amiloride is a potassium sparing duretic which specifically inhibits $Na{^+}$ channels. In the present study, we investigated the possible interaction of amiloride with $A_1$ adenosine receptors-adenylyl cyclase system in crude adipocytic plasma membrane fractions prepared from Sprague-Dawley rats. When the function of $G_i$ protein (inhibitory guanine nucleotide binding protein) was assessed by determining the effects of GTP on isoproterenol-stimulated adenylyl cyclase activity, the inhibitory effect of high concentrations of GTP was not observed in the presence of amiloride. In contrast, the adenosine receptor-mediated inhibition of the enzyme activity, as determined empolying 2-chloroadenosine, was either unchanged or even more enhanced by amiloride depending on the concentrations of 2-chloroadenosine. Thus, it appears that GTP- and receptor-mediated inhibitory function of $G_{i}$ proteins can be separated from one another. Receptor-mediated function of $G_{s}$ protein did not appear to be significantly affected by amiloride, since the inhibition of isoproterenol-stimulated adenylyl cyclase activity by propranolol under the same conditions was not significantly altered by amiloride. The enhancement of 2-chloroadenosine-mediated inhibition of adenylyl cyclase by amiloride was maintained in the presence of 150 mM NaCl. In summary, these results suggest that amiloride interacts both with $A_{l}$ adenosine receptors and with $G_i$ proteins in adipocytic membranes. Its binding to the $A_1$ adenosine receptors appears to facilitate the coupling of the receptors with $G_i$ proteins thereby enhancing the inhibition of isoproterenol-stimulated adenylyl cyclase activity by $A_1$ adenosine agonist, and the direct interaction with $G_i$ proteins appears to remove the GTP-dependent inhibitory effect on adenylyl cyclase activity.

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A Study on the estimation of traffic congestion for Pyeongtaek·Dangjin port development (평택·당진항의 내항개발에 따른 교통혼잡도 평가에 관한 연구)

  • Gug Seung-Gi;Kim Se-Won;Kim Jung-Hoon
    • Journal of Navigation and Port Research
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    • v.29 no.6 s.102
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    • pp.481-485
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    • 2005
  • The port entry system of the inner harbor in Pyeongtaek${\cdot}$Dangjin was planned as lock-gate in 'Master plan project on port planning in Asan industry base(1990)‘, but was changed to tidal harbor in 'Project maintaining Master plan for comprehensive development of Pyeongtaek${\cdot}$Dangjin port(2001)'. Accordingly, southern sea bank constructed under the lower part of Seohae-bridge will be removed so that inbound/outbound vessels for the inner harbor can navigate at all times. However, in the view of the safety on passing through the lower of Seohae-bridge, navigating conditions for the inner harbor will be restricted in the single-way of 50,000 DWT vessel and the two-way of vessel less than 30,000 DWT Therefore, this study carried out the estimation of traffic congestion arising from these vessels with above restrictions q[ter supposing annual inbound/outbound vessel's numbers for loading and unloading cargo surveyed on the inner harbor.

Numerical Analysis of Resin Filling Process for a Molded Dry-type Potential Transformer (몰드형 건식 계기용 변압기 제작을 위한 수지 충진 해석 연구)

  • Kim, Moosun;Jang, Dong Uk;Kim, Seung Mo
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.17 no.12
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    • pp.511-517
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    • 2016
  • Current oil-type potential transformers for trains are filled with insulating oil, which could have problems like explosions due to rising inner pressure during train operation. Therefore, mold and dry-type potential transformers are being developed to prevent explosions. One problem in manufacturing mold-type transformers is preventing void formation around the coiled core inside the mold during epoxy filling, which could cause an electrical spark. Micro voids can remain in the resin after filling, and macro voids can occur due to the structure shape. A transformer that is being developed has a cavity at the junction of the core and the coil for better performance, and when highly viscous epoxy flows inside the cavity channel, macro voids can form inside it. Therefore, in this study, the free-surface flow of the mold filling procedure was analyzed numerically by applying the VOF method. The results were used to understand the phenomena of void formation inside the cavity and to modify the process conditions to reduce voids.

Effect of Transverse Convex Curvature on Turbulent Fluid Flow in Fuel Channel (핵연료 수로내 난류 유동에 대한 횡방향 볼록구배의 영향)

  • Lee, Yung;Ahn, Seung-Hoon;Kim, Hyong-Chol
    • Nuclear Engineering and Technology
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    • v.26 no.3
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    • pp.440-452
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    • 1994
  • Nuclear fuel bundles are designed such that the heat flux at a-fuel pin surface should not exceed the critical heat flux (CHF) during normal operation and anticipated transient. Therefore, evaluation of the CHF for fuel bundle is demanded in an exact and reliable manner. One of the major concerns with the current application of CHF correlations is that the CHF based on circular tubes is applied to the fuel bundle subchannel analysis, mainly in terms of the hydraulic diameter with correction factors which may result in a source of possibly large uncertainties in CHF prediction. The hydraulic diameter does not recognize the local properties of fluid nor such effect as the surface curvature; the turbulence action on the convex surface is much more pronounced than that on the concave surface. Even for the tube having concave curvature, the effect of tube diameter on CHF becomes important with decreasing diameter. These facts imply that the convex curvature effect is significant and crucial to the reliable CHF prediction. This paper reviews and discusses analytical and experimental aspects of effect of transverse convex curvature in incompressible turbulent flow and heat transfer, and on CHF. Flow models to quantify this effect are briefly mentioned and future works are recommended.

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Assessment on Flood Characteristics Changes Using Multi-GCMs Climate Scenario (Multi-GCMs의 기후시나리오를 이용한 홍수특성변화 평가)

  • Son, Kyung-Hwan;Lee, Byong-Ju;Bae, Deg-Hyo
    • Journal of Korea Water Resources Association
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    • v.43 no.9
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    • pp.789-799
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    • 2010
  • The objective of this study is to suggest an approach for estimating probability rainfall using climate scenario data based GCM and to analyze changes of flood characteristics like probability rainfall, flood quantile and flood water level under climate change. The study area is Namhan river basin. Probability rainfalls which is taken 1440 minutes duration and 100-year frequency are estimated by using IPCC SRES A2 climate change scenario for each time period (S0: 1971~2000; S1: 2011~2040; S2: 2041~2070; S3: 2071~2100). Flood quantiles are estimated for 17 subbasins and flood water level is analyzed in the main channel from the downstream of Chungju dam to the upstream of Paldang dam. Probability rainfalls, peak flow from flood quantile and water depth from flood water level have increase rate in the range of 13.0~15.1 % based S0 (142.1 mm), 29.1~33.5% based S0 ($20,708\;m^3/s$), 12.6~13.6% in each S1, S2 and S3 period, respectively.