• Title/Summary/Keyword: $In_{2}O_{3}$

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Effects of Donor Cells and Estrus Synchronization on the Production of Cloned Korean Native Goat (공핵 세포 및 발정 동기화가 복제 재래 산양 생산에 미치는 영향)

  • Park H.S.;Kim T.S.;Jung S.Y.;Park J.K.;Lee J.S.;Jung J.Y.
    • Journal of Embryo Transfer
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    • v.21 no.2
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    • pp.137-146
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    • 2006
  • The objective of this study was to examine the effect of donor cell types, the source of recipient oocytes and estrous synchronization on pregnancy and delivery rates of somatic cell nuclear transfer (SCNT) embryos in Korean native goats. Recipient oocytes were surgically collected after superovulation. Ear cells and fetal fibroblasts were collected and cultured in serum-starvation condition (TCM-199 + 0.5% FBS) for cell confluence. The zonae pellucidae of in vivo- and in vitro-matured oocytes were partially drilled using a laser system. Single somatic cell was transferred into the enucleated oocyte. The reconstructed oocytes were electrically fused with 0.3 M mannitol. After the fusion, embryos were activated by Ionomycin+6-DMAP. NT embryos were cultured in mSOF medium supplemented with 0.8% BSA at $39^{\circ}C$ in an atmosphere of 5% $CO_2$, 5% $O_2$, 90% $N_2$ for 12 to 20 hr. One hundred and two SCNT embryos were transferred into 20 recipients and pregnancy rate at days 30 was 20.0%. Of them, one developed to term and delivered 1 kid. Ear cells showed significantly higher fusion (63.8 vs. 26.5%) and pregnancy rates (20.0 vs. 0.0%) than those of fetal fibroblast (p<0.05). The recipients synchronized by CIDR showed significantly lower pregnancy rates compared to that of recipient in natural estrus ($0.0{\sim}25.0%$ vs. 100%) (p<0.05). Cloned kid was born from the recipient in natural estrus. For the synchronization of estrus between recipient and donor, there was no difference between treatments (${\pm}0$ vs. +12 hr) in pregnancy rate. The first healthy cloned kid (Jinsoonny) was produced by transfer of SCNT embryos derived from in vivo oocytes and ear cells into a recipient goat whose estrus was synchronized with the donor. These results imply that donor cells for nuclear transfer may affect the success rate, and the estrus synchronization between donor and recipient animals can also be important.

Development of Pozzolanic material from clay

  • Alaskar, Abdulaziz;Shah, S.N.R.;Keerio, Manthar Ali;Phulpoto, Javed Ali;Baharom, Shahrizan;Assilzadeh, Hamid;Alyousef, Rayed;Alabduljabbar, Hisham;Mohamed, Abdeliazim Mustafa
    • Advances in concrete construction
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    • v.10 no.4
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    • pp.301-310
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    • 2020
  • The following paper concentrates on the objective of studying the influences of extent of duration and temperature on the Pozzolanic properties as well as reactivity of locally existing natural clay of Nai Gaj, district Dadu, Sindh Pakistan. The activation of the clay only occurs through heating when temperature in a furnace chamber reaches 600, 700 and 800oC for 1, 2 and 3 hours and at 900 and 1000℃ for 1 and 2 hours. Furthermore, the strength activity index (SAI) of advanced pozzolanic material happens to be identified through 20% cement replacement for different samples of calcined clay as per ASTM C-618. The compressive strength test of samples had been operated for 7 and 28-days curing afterwards. The maximum compressive strength had been seen in mix E in which cement was replaced with clay calcined at 700℃ for 1 hour that is 27.05 MPa that is 24.31% more than that of control mix. The results gathered from the SAI verdicts the optimal activation temperature is 700℃ within a one-hour time period. The SAI at a temperature of 700℃ with a one-hour duration at 28 days is 124.31% which happens to satisfy the requirements of the new Pozzolanic material, in order to be applied in mortar/concrete (i.e., 75%). The Energy- dispersive spectrometry (EDS) along with the X-ray diffraction (XRD) have been carried out in means of verifying whether there is silica content or amorphous silica present in metakaolin that has been developed. The findings gathered from the SAI were validated, as the analysis of XRD verified that there is in fact Pozzolanic activity of developed metakaolin. Additionally, based on observation, the activated metakaolin holds a significant influence on the increase in mortar's compressive strength.

Determination of Nitrogen Application Level for Chinese Cabbage with Application of Poultry Manure Compost in Highland (계분퇴비 시용시 고랭지 배추에 대한 질소 시비량 결정)

  • Lee, Choon-Soo;Shin, Kwan-Yong;Lee, Jeong-Tae;Lee, Gye-Jun;Ahn, Jae-Hoon
    • Korean Journal of Soil Science and Fertilizer
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    • v.36 no.5
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    • pp.280-289
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    • 2003
  • The purpose of this study was to establish a model for recommendable application level of nitrogen fertilizer based on soil testing for summer chinese cabbage in highland. A field experiment was carried out with various nitrogen application levels in sand loamy soil with and without poultry manure compost. The application level of N in poultry manure compost plot was found to be $291kg\;ha^{-1}$ for maximum yield of chinese cabbage, and it was 87% of the required N application level, $335kg\;ha^{-1}$, for maximum yield of chinese cabbage in nonmanure plot. In the treatment of poultry manure, approximately $174kg\;ha^{-1}$ of N was required to obtain the same yield of chinese cabbage as the maximum yield obtained in the treatments without poultry manure application. Therefore, with poultry manure application, N application level can be reduced by 40%. Using these results, a new equation for N recommendation for chinese cabbage in highland soil was proposed. With the average organic matter content of $33g\;kg^{-1}$ in highland field, the application levels of N for chinese cabbage were in the range of $215-129kg\;ha^{-1}$, which means that 32.8-59.7% of current application amount of N fertilizer can be reduced.

$In_{0.64}Al_{0.36}Sb$층의 성장온도 및 도핑에 따른 광학적 특성

  • O, Jae-Won;Kim, Hui-Yeon;Ryu, Mi-Lee;Im, Ju-Yeong;Sin, Sang-Hun;Kim, Su-Yeon;Song, Jin-Dong
    • Proceedings of the Korean Vacuum Society Conference
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    • 2010.02a
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    • pp.160-160
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    • 2010
  • 테라헤르츠 소스로 저온 InGaAs를 대체하기 위해 저온에서 성장한 $In_{0.64}Al_{0.36}Sb$의 성장 온도에 따른 광학적 photoluminescence (PL)과 time-resolved PL (TRPL) 측정을 이용하여 분석하였다. 또한 Be 도핑 농도에 따른 p형 $In_{0.64}Al_{0.36}Sb$의 PL과 TRPL 특성을 undoped $In_{0.64}Al_{0.36}Sb$와 Si-doped $In_{0.64}Al_{0.36}Sb$ 결과와 비교 분석하였다. 본 연구에 사용한 시료는 분자선 엑피탁시 (molecular beam epitaxy)법으로 GaAs 기판 위에 $In_{0.64}Al_{0.36}Sb$을 다양한 성장온도에서 ${\sim}3.7\;{\mu}m$두께 성장하였다. $In_{0.64}Al_{0.36}Sb$의 성장온도는 $400^{\circ}C$ 에서 $460^{\circ}C$까지 변화시키며 성장하였으며, Si과 Be 도핑한 $In_{0.64}Al_{0.36}Sb$ 시료는 약 $420^{\circ}C$에서 성장하였다. 모든 시료의 PL 피크는 ~1450 nm 근처에서 나타나며 단파장 영역에 shoulder 피크가 나타났다. 그러나 가장 낮은 온도 $400^{\circ}C$에서 성장한 시료는 1400 nm에서 1600 nm에 걸쳐 매우 넓은 피크가 측정되었다. PL 세기는 $450^{\circ}C$ 에서 성장한 시료가 가장 강하게 나타났으며, $435^{\circ}C$에서 성장한 시료의 PL 세기가 가장 약하게 나타났다. 방출파장에 따른 PL 소멸곡선을 측정하였으며 double exponential function을 이용하여 운반자 수명시간을 계산하였다. 운반자 수명시간은 빠른 소멸성분 $\tau_1$과 느린 소멸성분 $\tau_2$가 존재하고 빠른 성분 $\tau_1$의 PL 진폭이 약 80%로 느린 성분 $\tau_2$보다 우세하게 나타났다. 각 PL 피크에서의 운반자 수명시간 $\tau_1$은 ~1 ns로 성장온도에 따른 변화는 관찰되지 않았다. 또한 방출파장이 1400 nm에서 1480 nm까지 PL 피크 근처에서 운반자 수명시간은 거의 일정하게 나타났다. Be-doped 시료의 PL 피크는 1236 nm에서 나타나며, Si-doped 시료는 1288 nm, undoped 시료는 1430 nm에서 PL 피크가 측정되었다. PL 피크에서 PL 소멸곡선은 Be-doped 시료가 가장 빨리 감소하였으며, Si-doped 시료가 가장 길게 나타났다. 이러한 결과로부터 $In_{0.64}Al_{0.36}Sb$의 광학적 특성은 성장 온도, dopant type, 도핑 농도에 따라 변화하는 것을 확인하였다.

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Fresh-water Algae Occurred in Paddy Rice Fields II. Identification of Blue-green Algae (논발생(發生) 담수조류(淡水藻類)에 관(關)한 연구(硏究) II. 람조류(籃藻類)의 동정(同定))

  • Lee, H.K.;Park, J.E.;Ryu, G.H.;Lee, J.O.;Park, Y.S.
    • Korean Journal of Weed Science
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    • v.12 no.2
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    • pp.166-172
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    • 1992
  • The identification on blue-queen algae which were collected from paddy rice fields of the whole nation was carried out in 1991. The blue-green algae identified were 20 species in 5 families including 7 species in the Chroococcaceae. 1 species in the Chamaesiphonaceae. 1 species in the Pleurocapsaceae, 6 species in the Nostocaeae and 5 species in the Oscillatoriaceae. Among the blue-green algae, 6 species in 4 genera including Cylindrospernum, Anabaenopsis, Anabaena and Nostoc were characterized by heterocysts concerning with nitrogen fixation capacity. A concentrated population of Oscillatoria spp. induced soil flakes with blue tint in paddy rice fields. The genera. Spirulina and Oscillatoria, showed their specific motility.

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Sulfate Reduction in the Marine Environments: Its Controlling Factors and Relative Significance in Mineralization of Organic Matter (해양환경의 황산염 환원율 조절요인 및 유기물 분해에 있어 황산염 환원의 중요성)

  • 현정호;이홍금;권개경
    • The Sea:JOURNAL OF THE KOREAN SOCIETY OF OCEANOGRAPHY
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    • v.8 no.2
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    • pp.210-224
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    • 2003
  • Sulfate reduction is a microbiological process which occurs ubiquitously in anaerobic marine environment. Sulfate reducing bacteria play a significant role in anaerobic decomposition of organic matter and regeneration of inorganic nutrients which supports the primary production in the water column (i.e., benthic-pelagic coupling) and, in special case, could be responsible for the harmful algal bloom in the coastal marine environment. Summary of the sulfate reduction rates reported in various marine sedimentary environments revealed that supply of organic substrates and presence of various electron acceptors (i.e., $O_2$, NO$_{3}$$^{[-10]}$ , Fe(III) and Mn(IV), etc.) for other aerobic and anaerobic respiration directly affect the sulfate reduction rate and relative significance of sulfate reduction in organic matter mineralization. Significance of temperature, macrophytes and bioturbation is discussed as factors controlling supply of organic substrates and distribution of electron acceptors. Finally, we suggest studies on the anaerobic microbiological processes associated with biogeochemical element cycles in the coastal environments of Korea where massive operation of organic enriched fish cage farm, frequent occurrence of toxic algal bloom and hypoxia and conservation of tidal flat are of major environmental issues.

Agrobacterium-Mediated Transformation of Phalaenopsis by Using Protocorm-Like Body (Protocorm-like body를 이용한 호접란 형질전환 연구)

  • Hur, Yeon-Jae;Kim, Eun-Young;Yang, Won-Tae;Lee, Young-Byoung;Lee, Jae-Hun;Jung, Young-Soo;Nam, Jae-Sung;Yun, Dae-Jin;Yi, Ki-Hwan;Kim, Doh-Hoon
    • Journal of Life Science
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    • v.19 no.3
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    • pp.378-383
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    • 2009
  • Agrobacterium tumefaciens-mediated transformation procedure for the phalaenopsis orchid, established by using Protocorm-like bodies (PLBs), was aimed at the introduction of target genes into individuals with divergent genetic backgrounds. PLBs obtained from the axillary bud of a peduncle were maintained on a hyponex medium supplemented with 1 g/l of activated charcoal, 30 g/l of sucrose and 0.1 mg/l thiamine. The multiplication rate of PLBs was about 90% in case of subculture PLBs to be cut transversely into 1/3 part from top position. The PLBs were inoculated with Agrobacterium strain EHA105 harboring both $\beta$-glucuronidase (GUS) and hygromycin-resistant genes for 20 minutes after dipping treatment. Transformation efficiency was the highest with a Agrobacterium culture medium and dipping treatment of O.D. 0.8. Newly induced PLBs were put on selection medium containing 1 mg/l hygromycin for 2 months. Hygromycin-resistant phalaenopsis plants that regenerated after the selection culture of PLBs showed histochemical blue staining due to GUS. Transgene integration of the hygromycin-resistant plants was confirmed by PCR and Southern blot using GUS specific primers and probe.

A STUDY ON THE TEMPERATURE CHANGES OF BONE TISSUES DURING IMPLANT SITE PREPARATION (임플랜트 식립부위 형성시 골조직의 온도변화에 관한 연구)

  • Kim Pyung-Il;Kim Yung-Soo;Jang Kyung-Soo;Kim Chang-Whe
    • The Journal of Korean Academy of Prosthodontics
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    • v.40 no.1
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    • pp.1-17
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    • 2002
  • The purpose of this study is to examine the possibility of thermal injury to bone tissues during an implant site preparation under the same condition as a typical clinical practice of $Br{\aa}nemark$ implant system. All the burs for $Br{\aa}nemark$ implant system were studied except the round bur The experiments involved 880 drilling cases : 50 cases for each of the 5 steps of NP, 5 steps of RP, and 7 steps of WP, all including srew tap, and 30 cases of 2mm twist drill. For precision drilling, a precision handpiece restraining system was developed (Eungyong Machinery Co., Korea). The system kept the drill parallel to the drilling path and allowed horizontal adjustment of the drill with as little as $1{\mu}m$ increment. The thermocouple insertion hole. that is 0.9mm in diameter and 8mm in depth, was prepared 0.2mm away from the tapping bur the last drilling step. The temperatures due to countersink, pilot drill, and other drills were measured at the surface of the bone, at the depths of 4mm and 8mm respectively. Countersink drilling temperature was measured by attaching the tip of a thermocouple at the rim of the countersink. To assure temperature measurement at the desired depths, 'bent-thermocouples' with their tips of 4 and 8mm bent at $120^{\circ}$ were used. The profiles of temperature variation were recorded continuously at one second interval using a thermometer with memory function (Fluke Co. U.S.A.) and 0.7mm thermocouples (Omega Co., U.S.A.). To simulate typical clinical conditions, 35mm square samples of bovine scapular bone were utilized. The samples were approximately 20mm thick with the cortical thickness on the drilling side ranging from 1 to 2mm. A sample was placed in a container of saline solution so that its lower half is submerged into the solution and the upper half exposed to the room air, which averaged $24.9^{\circ}C$. The temperature of the saline solution was maintained at $36.5^{\circ}C$ using an electric heater (J. O Tech Co., Korea). This experimental condition was similar to that of a patient s opened mouth. The study revealed that a 2mm twist drill required greatest attention. As a guide drill, a twist drill is required to bore through a 'virgin bone,' rather than merely enlarging an already drilled hole as is the case with other drills. This typically generates greater amount of heat. Furthermore, one tends to apply a greater pressure to overcome drilling difficulty, thus producing even greater amount heat. 150 experiments were conducted for 2mm twist drill. For 140 cases, drill pressure of 750g was sufficient, and 10 cases required additional 500 or 100g of drilling pressure. In case of the former. 3 of the 140 cases produced the temperature greater than $47^{\circ}C$, the threshold temperature of degeneration of bone tissue (1983. Eriksson et al.) which is also the reference temperature in this study. In each of the 10 cases requiring extra pressure, the temperature exceeded the reference temperature. More significantly, a surge of heat was observed in each of these cases This observations led to addtional 20 drilling experiments on dense bones. For 10 of these cases, the pressure of 1,250g was applied. For the other 10, 1.750g were applied. In each of these cases, it was also observed that the temperature rose abruptly far above the thresh old temperature of $47^{\circ}C$, sometimes even to 70 or $80^{\circ}C$. It was also observed that the increased drilling pressure influenced the shortening of drilling time more than the rise of drilling temperature. This suggests the desirability of clinically reconsidering application of extra pressures to prevent possible injury to bone tissues. An analysis of these two extra pressure groups of 1,250g and 1,750g revealed that the t-statistics for reduced amount of drilling time due to extra pressure and increased peak temperature due to the same were 10.80 and 2.08 respectively suggesting that drilling time was more influenced than temperature. All the subsequent drillings after the drilling with a 2mm twist drill did not produce excessive heat, i.e. the heat generation is at the same or below the body temperature level. Some of screw tap, pilot, and countersink showed negative correlation coefficients between the generated heat and the drilling time. indicating the more the drilling time, the lower the temperature. The study also revealed that the drilling time was increased as a function of frequency of the use of the drill. Under the drilling pressure of 750g, it was revealed that the drilling time for an old twist drill that has already drilled 40 times was 4.5 times longer than a new drill The measurement was taken for the first 10 drillings of a new drill and 10 drillings of an old drill that has already been used for 40 drillings. 'Test Statistics' of small samples t-test was 3.49, confirming that the used twist drills require longer drilling time than new ones. On the other hand, it was revealed that there was no significant difference in drilling temperature between the new drill and the old twist drill. Finally, the following conclusions were reached from this study : 1 Used drilling bur causes almost no change in drilling temperature but increase in drilling time through 50 drillings under the manufacturer-recommended cooling conditions and the drilling pressure of 750g. 2. The heat that is generated through drilling mattered only in the case of 2mm twist drills, the first drill to be used in bone drilling process for all the other drills there is no significant problem. 3. If the drilling pressure is increased when a 2mm twist drill reaches a dense bone, the temperature rises abruptly even under the manufacturer-recommended cooling conditions. 4. Drilling heat was the highest at the final moment of the drilling process.

Comparison of Feed Efficiency Between Rotifers Enriched Lipid-contents to Enrichment and Enhanced Digestive Enzymes Activity to Starch (영양강화 Rotifer와 효소활성 향상 Rotifer의 먹이효율 비교)

  • Kwon, O-Nam;Park, Heum-Gi
    • Journal of Aquaculture
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    • v.22 no.1
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    • pp.105-111
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    • 2009
  • In this study, we carried out an experiment for estimation the larval digestibility in aspects which digestive enzymatic activities and nutrition of the rotifers, Brachionus rotundiformis. Thus we enhanced the digestive enzymatic activity through the addition of starch for the increase of digestibility of rotifer (starch-rotifer), and compared with the feed efficiency through rearing of the olive flounder, Paralichthys olivaceus used rotifer lipid-enriched with Algamac $2000^{(R)}$ (CE-rotifer). The digestive enzyme activities (except for TG-lipase), total protein contents, total essential amino acid, essential amino acids (methionin and phenylalanine) of starch-rotifer (the rotifer used a starch as additive, and enriched not) was assayed significantly higher than CE-rotifer (P<0.05). And total lipid, lipid classes (except for sterol) and fatty acids as DHA and EPA showed higher in CE-rotifer than starch-rotifer (P<0.05). But, sterol contents and ST/TG ratio were shown significantly higher in starch-rotifer (P<0.05). The flounder larvae supplied the two rotifers showed standard length and body weight that not significantly differed with ranges $3.72{\sim}3.79\;mm$ and $32.9{\sim}37.8\;mg$/larva on 6 days after hatching (DAH), respectively (P>0.05). However, these of 12 DAH showed the values of significantly higher to $5.94{\pm}0.249\;mm$, $144.0{\pm}23.86\;mg$/larva and $26.2{\pm}12.13%$ in standard length, body weight and survival in CE-flounder than that of starch-flounder (P<0.05). The hydrolytic enzymatic activities of flounder larvae severally supplied the two rotifers showed the significantly higher activities in acidic -amylase, neutral -amylase, TG-lipase, lysozyme and acidic phosphatase in starch-flounder on 5 DAH (P<0.05). But neutral $\alpha$-amylase, three proteases and two phosphatases of CE-flounder on 11 DAH showed the significantly higher activities than that of starch-flounder (P<0.05). Therefore, for the flounder, Paralichthys olivaceus larvae just depleted yolk was more beneficial to supply the feed, rotifer, enhanced the digestibility than to supply the feed lipid-enriched for aspect of larval digestibility up to 6 DAH, thereafter nutrition of absorption due to the development of digestive organs suggested that enrichment effect appeared with larval somatic growth. Consequently, investigation more detailed about the larval digestive physiological and nutritional requirement variations after 6 DAH will be necessary, thereafter.

Analysis of the Forest Vegetation of Mt. Ilwol (일월산의 삼림식생 분석)

  • Lee, Ho-Joo;Heung-Lak Choung;Doo-Weon Byun;Chang-Ho Kim
    • The Korean Journal of Ecology
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    • v.16 no.3
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    • pp.239-259
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    • 1993
  • The vegetation types and distributional characteristics of the forest vegetation on Mt. Ilwol were investigated by the Z-M school method. Geographical distribution of the vegetation was a central/montane(CEMO) type of the Korean Peninsula subtype. The forest was classified into five vegetation units: Artemisio-Quercetum mongolicae (Artemisio-Quercetum typicum subass. nov., Artemsio-Quercetum pinetosum dens~florae subass. nov. ). Lychno-Quercetum mongolicae (Lychno-Quercetum Calamagrostetosum arundinaceae subass. nov.. Lychno-Quercetum ligularetosum f~scherii subass. nov.), Fraxinus rhynchophylla community, Quercus variabilis community and Larix leptolepis afforestation. Species with percentage constance degree more than 61% are Quercus mongolica (89.5%, V), Lespedeza maximowiczii (73.7%, IV), Isodon japonicus(73.7%, IV), Fraxinus rhynchophylla (68.%, IV), Arlemisia stolonifera (65, 8%, IV), etc. Artemisio-Quercetum mongolicae was chiefly distributed on the ridge lines of low altitudes (570~700m) and on the ground with exposed rocks in sloping surface (800~1, 000m). Both Fraxinus sievoldiana and Rhododendrou schlippenbachii of the Artemisio-Quercetum mongolicae showed a predominant condition all over the subtree and shrub layers. Of Artemisio-Quercetum pinetosum densifloraes, Pinus densiflora becomes powerful over the tree layer and, on the other hand, Quercus mongolica over the subtree layer. Lychno-Quercetum mongolicae was distributed somewhat on the humid region of the high altitude area (840~1.200m). Fraxinus rhynchophylla community was chiefly distributed in the vicinity of the ravines. Quereus rariabilis community was distributed at subxeric slope on southeast side of the mountain. Larix leptolepis has been reforested at the ecotone between Artemisio-Quercetum mongolicae and Lychno-Quercetum mongolicae. Percentage cover degrees of the subtree layer In Lychno-Quercetum mongolicae, Quereus rariabilis community and Larix leptolepis afforestation were relatively low, 15.3%, 5% and 2.5%, respectively.The contents of moisutre and organic matter in the soil were much higher in Luchno-Quercetum monogolicae and Larix leptolepis afforestaion than in the other communities. Contents o Ca, Mg, K and the available phosphorus were relaively high compared with forests of the other area. This result seems to be influenced by a forest fire. As the altitude increases, the moisure content in the forest soil also increases.

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