• 한방비만학회지
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• 제21권2호
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• pp.59-68
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• 2021

Objectives: We investigated the effects of Clean-DM4 (C-DM4), a polyherbal extract consisting of Coptidis Rhizoma, Salviae Miltiorrhiza Radix, and Cinnamomi Cortex on high fat diet (HFD)-induced obesity and diabetes in mice. Methods: The C57BL/6 mice (6 weeks) were fed a HFD for 8 weeks and then administrated with C-DM4 extract at 500 mg/kg (p.o.) once daily for 4 weeks. The changes of body weights, calorie intakes, and fasting blood glucose (FBG) levels were measured in mice. The serum levels of glucose, insulin, total cholesterol, aspartate aminotransferase (AST), and alanine aminotransferase (ALT) were measured in mice by enzyme-based assay. It was also observed the histological changes of pancreas, liver, and fat tissues with hematoxylin and eosin (H&E) staining. Results: The increase of calorie intakes and FBG levels in HFD-induced obesity mice was significantly decreased by oral administration of C-DM4 extract. C-DM4 extract administration was significantly reduced the increased levels of glucose, insulin, total cholesterol, AST, and ALT in obesity mice. In addition, C-DM4 extract inhibited lipid droplet accumulation in liver tissues of obesity mice, hyperplasia of pancreatic islets, and enlargement of adipocytes in adipose tissues. Conclusions: Our study indicates that C-DM4 extract could help improve obesity and to prevent diabetes progression.

• 대한본초학회지
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• 제32권6호
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• pp.63-69
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• 2017

Objectives : The purpose of this study is to investigate the preventive and therapeutic efficacy of osteoarthritis using a Yeonsan-Ogye egg. so, we researched at effects of Yeonsan-Ogye egg extract on MIA-induced Osteoarthritis animal models. Methods : Yeonsan-Ogye egg extract was administered 500 mg/kg/day, 1000 mg/kg/day and 2000 mg/kg/day to SD-Rat for 2 weeks. After that, osteoarthritis was induced with $60mg/m{\ell}$ of monosodium iodoacetate (MIA) and futher administration was continued for 4 weeks. 3D imaging of cartilage patella were obtained using a Micro-CT system and the pathology change of knee was observed by H&E and safranin-O staining. The weight bearing ratio was measured by incapacitance test meter. MMP-2, MMP-9, COMP, CTX II, calcitonin and glycosaminoglycan level in serum were measured using a ELISA. Results : Micro-CT and Histopathological analysis showed the volume of the patella cartilage and the proteoglycan contents were increased in all groups. also weight bearing ratio was decreased in all groups compared with control group. Calcitonin production was increased in and 2000 mg/kg/day group and glycosaminoglycan production was increased in all groups. In addition, MMP-2, MMP-9, COMP and CTX II production were decreased in 1000 and 2000 mg/kg/day groups respectively in comparison with control. Conclusions : The results for Yeonsan-Ogye egg showed prevention and treatment efficacy against arthritis at serum and the cartilage. These results may be used a remedy for new korea medicine to ease the symptoms mentioned above. also, suggest that Yeonsan-Ogye egg can be used preventive and therapeutic material for osteoarthritis.

• 한국환경과학회지
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• 제31권8호
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• pp.715-725
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• 2022

Lactic Acid Bacteria (LAB) are among the representative probiotics that have been used for a long time in fermented food. Although there are many studies on detecting the radical scavenging activity of LAB, few studies have been conducted on the environmental factors that improve scavenging activity. This study investigated the environmental factors affecting the DPPH radical scavenging and various antioxidant activities of Kimchi-derived Lactobacillus plantarum K-21 with antihypertensive and radical scavenging activities. The optimal conditions for scavenging DPPH radicals were glucose 2%, bactopeptone 0.5%, Tween 80 0.05%, L-cysteine 0.05%, and an initial pH 6.5 at 35℃. Under optimal conditions, the DPPH radical scavenging activity was 94.8±2.2%, which was 1.5 times higher than that of the basic medium. In addition, L. plantarum K-21 had other antioxidant activities; ABTS radical scavenging (93.6±1.5%), hydroxyl radical scavenging (8.5±0.9%), metal chelating (65.9±0.5%), NO scavenging (53.1±19%), SOD-like (25.1±1.5%), and reducing power (11.7±1.4%) activities were detected. Therefore, L. plantarum K-21 may act not only as a starter for lactic acid-fermented foods with improved functionality but also as a drug for various diseases caused by oxidative stress.

• 한국수산과학회지
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• 제18권5호
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• pp.491-495
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• 1985

1984년 11월 2일부터 1985년 2월 3일까지 부산수산대학 어류양식 연구실에서 평균 73.3g 및 26.7g되는 이스라엘잉어 각각 15미(총중량 1,100g및 400g)씩을 수용하고, 우리 나라에서 질이 우수하다고 인정되고 있는 상품사료와 $40\%$ 대두박분 대체사료를 대조구 사료로 하고, 인산일수소나트륨($Na_{2}HPO_{4}{\cdot}12H_{2}O$)을 $2\%$ 첨가하여 만든 인첨가 $40\%$ 대두박분 대체사료를 실험사료구로 하여 사료효율 시험을 하였다. 상품사료에서는 큰 개체군과 작은 개체군에서 각각 사료계수 1.340, 1.368, 1일 성장률 $1.696\%,\;1.708%$로 나타났고, 인첨가 $40\%$ 대두박분 대체사료에서는 사료계수 1.431, 1.558, 1일 성장율 $1.619\%,\;1.589\%$로 성장률이 상품사료에 비해 약간 떨어지기는 했지만 거의 비슷한 결과를 나타내었다. 그런데 $40\%$ 대두박분 대체사료는 사료계수 1.863, 1.820, 1일 성장률 $1.365\%,\;1.438\%$로 성장률이 크게 뒤떨어진 결과를 나타내었다. 그러므로 이상의 실험결과에 의하면 $40\%$ 대두박분 대체사료는 잉어의 사료로서는 적절하지 못했지만, 이에 적당량의 인을 첨가하게 되면 우수한 사료로 될 수 있다는 것을 밝혀주고 있다.

• Journal of Ginseng Research
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• 제41권2호
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• pp.169-179
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• 2017

Background: Ginsenosides are the main pharmacological components of Panax ginseng root, which are thought to be primarily responsible for the suppressing effect on oxidative stress. Methods: 2,2-diphenyl-1-picrylhydrazyl radical scavenging activity and oxygen radical absorption capacity were applied to evaluate the antioxidant activities of the ginsenosides. Human embryonic kidney 293 (HEK-293) cells were incubated with ginsenosides extracted by pulsed electric field (PEF) and solvent cold soak extraction (SCSE) for 24 h and then the injury was induced by $40{\mu}M$ $H_2O_2$. The cell viability and surface morphology of HEK-293 cells were studied using MTS assay and scanning electron microscopy, respectively. Dichloro-dihydro-fluorescein diacetate fluorescent probe assay was used to measure the level of intracellular reactive oxygen species. The intracellular antioxidant activities of ginsenosides were evaluated by cellular antioxidant activity assay in HepG2 cells. Results: The PEF extracts displayed the higher 2,2-diphenyl-1-picrylhydrazyl radical scavenging activity and stronger oxygen radical absorption capacity (with an oxygen radical absorption capacity value of $14.48{\pm}4.04{\mu}M\;TE\;per\;{\mu}g/mL$). The HEK-293 cell model also suggested that the protective effect of PEF extracts was dose-dependently greater than SCSE extracts. Dichloro-dihydro-fluorescein diacetate assay further proved that PEF extracts are more active (8% higher than SCSE extracts) in reducing intracellular reactive oxygen species accumulation. In addition, scanning electron microscopy images showed that the HEK-293 cells, which were treated with PEF extracts, maintained more intact surface morphology. Cellular antioxidant activity values indicated that ginsenosides extracted by PEF had stronger cellular antioxidant activity than SCSE ginsenosides extracts. Conclusion: The present study demonstrated the antioxidative effect of ginsenosides extracted by PEF in vitro. Furthermore, rather than SCSE, PEF may be more useful as an alternative extraction technique for the extraction of ginsenosides with enhanced antioxidant activity.

• 한국토양비료학회지
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• 제47권6호
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• pp.437-442
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• 2014

Foodborne illness due to the consumption of contaminated raw strawberries is a continuing food safety concern. This study investigated and evaluated contamination levels of bacteria on strawberries at farms stage to evaluate potential hazards associated with fresh strawberries. A total of 315 samples, 105 samples from 5 sampling sites (A to E) of 21 farms and 210 samples from 1 sampling site of 6 farms, was collected every month for four months and analyzed to enumerate aerobic bacterial counts, Coliforms/E. coli, Bacillus cereus and Staphylococcus aureus. In addition, the prevalence study of five pathogens (S. aureus, E. coli, E. coli O157:H7, Salmonella spp. and Listeria monocytogenes) was performed on each sample. Aerobic bacterial counts ranged from 0.48 to 6.36 Log CFU/g, with the highest bacterial cell counts recorded for D and E sites. Coliforms were detected in 71 samples (22.5%) with a minimum of 0.48 cfu/g and a maximum of more than 4 Log CFU/g. B. cereus was detected in 98 samples (31.1%) among total samples analyzed. S. aureus was detected in 2 samples with a minimum of 0.48 Log CFU/g and a maximum of 1.38 Log CFU/g. E. coli, E. coli O157:H7, Salmonella spp. and L. monocytogenes were not isolated from any of the samples. The microbial contamination levels of strawberries determined in this study may be used as the fundamental data for microbiological risk assessment.

• 한국식품저장유통학회지
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• 제17권2호
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• pp.196-201
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• 2010

자몽종자추출물(GSE)이 다른 농도(0, 0.6, 1.0%)로 첨가된 젤라틴 필름을 제조하여 필름의 물성과 딸기의 포장 효과를 각각 조사하였다. GSE의 농도가 증가함에 따라 젤라틴 필름의 신장률은 증가하였으나 인장강도는 대조구의 46.39 MPa에서 1.0% GSE 함유 젤라틴 필름 경우에 31.96 MPa로 감소하였고, 필름의 투습도는 GSE의 농도에 의해 영향을 받지 않았다. GSE 1.0%가 함유된 젤라틴 필름은 E. coli O157:H7과 L. monocytogenes의 성장을 억제하였다. GSE 1.0%가 함유된 젤라틴 필름을 딸기 포장에 적용하였을 때 저장 12일 차에서 대조구에 비해 총 호기성 세균, 효모 및 곰팡이의 수가 각각 1.60, 1.43 log CFU/g 낮은 값을 나타내었다. 또한 1.0% GSE-젤라틴 필름으로 포장한 딸기가 대조구나 젤라틴 필름만으로 포장한 딸기에 비해 관능적 품질이 우수하였다. 따라서 1.0% GSE-젤라틴 필름 포장이 딸기의 저장 중 미생물학적 위해인자의 감소와 딸기의 품질유지에 효과적인 방법이라고 판단된다.

• 한국생물공학회:학술대회논문집
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• 한국생물공학회 2003년도 생물공학의 동향(XII)
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• pp.40-44
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• 2003

Soil-borne infectious disease including Pythium aphanidermatum and Rhizoctonia solani causes severe damage to plants, such as cucumber. This soil-borne infectious disease was not controlled effectively by chemical pesticide. Since these diseases spread through the soil, chemical agents are usually ineffective. Instead, biological control, including antagonistic microbe can be used as a preferred control method. An efficient method was developed to select an antagonistic strain to be used as a biological control agent strain. In this new method, surface tension reduction potential of an isolate was included in the ‘decision factor’ in addition to the other factors, such as growth rate, and pathogen inhibition rate. Considering these 3 decision factors by a statistical method, an isolate from soil was selected and was identified as Bacillus sp. GB16. In the pot test, this strain showed the best performance among the isolated strains. The lowest disease incidence rate and fastest seed growth was observed when Bacillus sp. GB16 was used. Therefore this strain was considered as plant growth promoting rhizobacteria (PGPR). The action of surface tension reducing component was deduced as the enhancement of wetting, spreading, and residing of antagonistic strain in the rhizosphere. This result showed that new selection method was significantly effective in selecting the best antagonistic strain for biological control of soil-borne infectious plant pathogen. The antifungal substances against P. aphanidermatum and R. solani were partially purified from the culture filtrates of Bacillus sp. GB16. In this study, lipopeptide possessing antifungal activity was isolated from Bacillus sp. GB16 cultures by various purification procedures and was identified as a surfactin-like lipopeptide based on the Fourier transform infrared spectroscopy (FT-IR), nuclear magnetic resonance (NMR), high performance liquid chromatography mass spectroscopy (HPLC-MS), and quadrupole time-of-flight (Q-TOF) ESI-MS/MS data. The lipopeptide, named GB16-BS, completely inhibited the growth of Pythium aphanidermatum, Rhizoctonia solani, Penicillium sp., and Botrytis cineria at concentrations of 10 and 50 mg/L, respectively. A novel method to prevent the foaming and to provide oxygen was developed. During the production of surface active agent, such as lipopeptide (surfactin), large amount of foam was produced by aeration. This resulted in the carryover of cells to the outside of the fermentor, which leads to the significant loss of cells. Instead of using cell-toxic antifoaming agents, low amount of hydrogen peroxide was added. Catalase produced by cells converted hydrogen peroxide into oxygen and water. Also addition of corn oil as an oxygen vector as well as antifoaming agent was attempted. In addition, Ca-stearate, a metal soap, was added to enhance the antifoam activity of com oil. These methods could prevent the foaming significantly and maintained high dissolved oxygen in spite of lower aeration and agitation. Using these methods, high cell density, could be achieved with increased lipopeptide productivity. In conclusion to produce an effective biological control agent for soil-borne infectious disease, following strategies were attempted i) effective screening of antagonist by including surface tension as an important decision factor ii) identification of antifungal compound produced from the isolated strain iii) novel oxygenation by $H_2O_2-catalase$ with vegetable oil for antifungal lipopeptide production.

• 한국잡초학회지
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• 제7권3호
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• pp.329-336
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• 1987

Glyphosate의 수종(數種) 제초제간(除草劑間)의 혼용(混用)에 따른 상호작용(相互作用)을 검토(檢討)할 목적(目的)으로 겨풀에 대한 경엽처리효과(莖葉處理效果)와 피를 위시한 기타 잡초종(雜草種)에 대한 발생전처리효과(發生前處理效果)를 동시에 평가(評價)하였다. 폿트를 이용하여 plastic house에서 수행(遂行)되었으며, 주요(主要) 결과(結果)를 다음과 같이 요약(要約)할 수 있다. 1. 약제처리후(藥劑處理後)의 경시적(經時的)인 달관평가(達觀評價)에 의하면, glyphosate에 oxyfluorfen을 혼용(混用)함으로써 겨풀의 살초속도(殺草速度)와 살초효과(殺草效果)가 모두 향상(向上)되었으나 alachlor, simazine, gluphosinate 또는 quizalofop-ethyl의 혼용(混用)으로는 약효발현(藥效發現)이 지연되었고, paraquat 혼용(混用)으로는 효과(效果)가 빨랐던 반면(反面) 약효(藥效)는 떨어지는 경향(傾向)이었다. 2. 처리후(處理後) 30일(日)에의 겨풀 생체중(生體重)으로 보아, 공시약제(供試藥劑) 각각의 고약량(高藥量) 수준(水準)(실용(實用)의 1/2) 혼용(混用)에서는 alachlor 또는 paraquat 이외의 모든 조합(組合)에 서 glyphosate 단제(單劑) 보다 비등(比等) 이상(以上)의 살초력(殺草力)을 나타내었다. 3. Glyphosate에 대한 공시(供試) 약제(藥劑)의 어떤 조합(組合) 혼용(混用)에서도 synergism은 인정(認定)되지 않았고, 오히려 저약량수준(低藥量水準)의 simazme이나 paraquat 혼용(混用)에서는 유의적(有意的)인 길항작용(拮抗作用)이 인정(認定)되었다. 4. 피 및 기타 초종(草種)에 대한 토양잔류성(土壤殘留性) 제초효과(除草效果)는 glyphosate와의 혼용(混用) 여부(與否)에 관계없이 oxyfluorfen, alachlor, simazine 및 일부 quizalofop-ethyl이 처리(處理)된 곳에서 인정(認定)이 되었다.

• Asian-Australasian Journal of Animal Sciences
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• 제25권4호
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• pp.515-523
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• 2012

The objective of this study was to investigate the variability in broiler performance, apparent metabolisable energy (AME) and ileal digestible energy (IDE) between five different maize samples fed with and without xylanase at 16,000 U/kg. Various in vitro characterisations were conducted to determine if any could predict performance or AME. Samples of the maize were harvested in five diverse regions and fed individually in a mash diet as follows (g/kg): test maize 608.3; soya bean meal (SBM) 324.1; poultry fat 25.2; salt 4.6; met 2.6; lys 1.6; thr 0.5; limestone 9.7, dical 18.4; vit/min 5.0; CP 210 and ME (kcal/kg) 3,085. The diets were fed to 720 broilers with 6 replicates, each containing 12 birds per treatment, from 0 to 18 d of age. Maize samples were analysed for starch, protein, crude fibre, fat, protein solubility index (PSI) and vitreousness using near infra red reflectance spectroscopy (NIR). They were also assayed using an in vitro starch digestibility method. The results showed that there was no effect of harvest region on the feed intake (FI), body weight gain (BWG) or feed conversion ratio (FCR) of the broilers over the 18 d period (p = 0.959, 0.926, 0.819 respectively). There was an improvement in all parameters with the addition of xylanase (FI p = 0.011; BWG and FCR p<0.001). There was a significant positive effect of xylanase on IDE, AME, IDE Intake (IDEI) and AME intake (AMEI) (p<0.0001 in all cases). Although there was no significant effect of maize source, there was a strong trend towards variability in IDE (p = 0.066) and AME (p = 0.058). There were no significant correlations (p<0.05) between any proximate or physiochemical values and any performance or AME values. This may suggest that none of those selected were suitable predictors for performance or AME. The broilers performed well according to the breed guidelines, with slightly increased FI, increased BWG and similar FCR prior to the addition of xylanase. When FCR and BWG were analysed with FI as a covariate, xylanase addition remained significant suggesting that the improvement in BWG and FCR was driven by an increase in digestibility and nutrient availability.