Browse > Article
http://dx.doi.org/10.15429/jkomor.2021.21.2.59

Anti-Obesity and Anti-Diabetic Effects of a Polyherbal Extract Consisting of Coptidis Rhizoma, Salviae Miltiorrhizae Radix, and Cinnamomi Cortex in High Fat Diet-Induced Obesity Mice  

Jung, Su Min (Department of Herbology, College of Korean Medicine, Dongguk University)
Kwon, Se Eun (Department of Herbology, College of Korean Medicine, Dongguk University)
Kang, Seok Yong (Korean Medicine R&D Institute, Dongguk University)
Kim, Su Jin (Department of Anesthesiology and Pain Medicine, Dongguk University Gyeongju Hospital, College of Medicine, Dongguk University)
Jung, Hyo Won (Department of Herbology, College of Korean Medicine, Dongguk University)
Park, Yong-Ki (Department of Herbology, College of Korean Medicine, Dongguk University)
Publication Information
Journal of Korean Medicine for Obesity Research / v.21, no.2, 2021 , pp. 59-68 More about this Journal
Abstract
Objectives: We investigated the effects of Clean-DM4 (C-DM4), a polyherbal extract consisting of Coptidis Rhizoma, Salviae Miltiorrhiza Radix, and Cinnamomi Cortex on high fat diet (HFD)-induced obesity and diabetes in mice. Methods: The C57BL/6 mice (6 weeks) were fed a HFD for 8 weeks and then administrated with C-DM4 extract at 500 mg/kg (p.o.) once daily for 4 weeks. The changes of body weights, calorie intakes, and fasting blood glucose (FBG) levels were measured in mice. The serum levels of glucose, insulin, total cholesterol, aspartate aminotransferase (AST), and alanine aminotransferase (ALT) were measured in mice by enzyme-based assay. It was also observed the histological changes of pancreas, liver, and fat tissues with hematoxylin and eosin (H&E) staining. Results: The increase of calorie intakes and FBG levels in HFD-induced obesity mice was significantly decreased by oral administration of C-DM4 extract. C-DM4 extract administration was significantly reduced the increased levels of glucose, insulin, total cholesterol, AST, and ALT in obesity mice. In addition, C-DM4 extract inhibited lipid droplet accumulation in liver tissues of obesity mice, hyperplasia of pancreatic islets, and enlargement of adipocytes in adipose tissues. Conclusions: Our study indicates that C-DM4 extract could help improve obesity and to prevent diabetes progression.
Keywords
Obesity; Type 2 diabetes mellitus; High-fat diet; Coptidis rhizoma extract; Salvia Miltiorrhiza; Cinnamomi Cortex;
Citations & Related Records
연도 인용수 순위
  • Reference
1 Xiao S, Zhang Z, Chen M, Zou J, Jiang S, Qian D, et al. Xiexin Tang ameliorates dyslipidemia in high-fat diet-induced obese rats via elevating gut microbiota-derived short chain fatty acids production and adjusting energy metabolism. J Ethnopharmacol. 2019 ; 241 : 112032.   DOI
2 Kim GH, Hong KK, Cho HB, Choi CM, Kim SB. Anti-inflammatory effects of Salvia Miltiorrhizae Radix water extract in RAW 264.7 cells and mouse induced by lipopolysaccharide. J Korean Obstet Gynecol. 2019 ; 32(2) : 1-17.   DOI
3 Barazzoni R, Cappellari GG, Ragni M, Nisoli E. Insulin resistance in obesity: an overview of fundamental alterations. Eat Weight Disord. 2018 ; 23(2) : 149-57.   DOI
4 Popkin BM, Adair LS, Ng SW. Global nutrition transition and the pandemic of obesity in developing countries. Nutr Rev. 2012 ; 70(1) : 3-21.   DOI
5 The Association of Korean Medicine. Obesity [Internet]. Seoul: 2009 [cited 2021 Aug 2]. Available from: https://www.akom.org/Home/AkomArticleHealth/105320?Healt hType=3.
6 Jung SM, Seol YH, Chun KY, Park MH, Liu Y, Kang SY, et al. Antiobesity and antidiabetic effects of polyherbal extract with Atractylodis Rhizoma, Anemarrhenae Rhizoma, Cinnamomi Cortex, and Moutan Radicles Cortex in high fat diet-induced obesity mice. J Korean Med Obes Res. 2020 ; 20(2) : 69-77.   DOI
7 Byun SH, Seo BI. Oriental medicines effects on cure and prevention of an obesty (I). J Korean Med. 2000 ; 21(1) : 3-10.
8 Korean Society for the Study of Obesity. Treatment guidelines for obesity. Seoul : Korean Society for the Study of Obesity. 2018.
9 Kang NH, Mukherjee S, Yun JW. Trans-cinnamic acid stimulates white fat browning and activates brown adipocytes. Nutrients. 2019 ; 11(3) : 577.   DOI
10 Zhao W, Yuan Y, Zhao H, Han Y, Chen X. Aqueous extract of Salvia miltiorrhiza Bunge-Radix Puerariae herb pair ameliorates diabetic vascular injury by inhibiting oxidative stress in streptozotocin-induced diabetic rats. Food Chem Toxicol. 2019 ; 129 : 97-107.   DOI
11 The Society of Korean Medicine Rehabilitaion. Korean rehabilitation medicine. Seoul : Koonja publisher. 2005 : 349-59.
12 Mahler RJ. The relationship between the hyperplastic pancreatic islet and insulin insensitivity in obesity. Acta Diabetol Lat. 1981 ; 18(1) : 1-17.   DOI
13 Yoon HJ, Lee YH, Cha BS. Causal relationship of non-alcoholic fatty liver disease with obesity and insulin resistance. J Korean Diabetes. 2014 ; 15(2) : 76-81.   DOI
14 Willams CL, Campanaro LA, Squillace M, Bollella M. Management of childhood obesity pediatric practice. Ann N Y Acad Sci. 1997 ; 817 : 225-40.   DOI
15 Donnelly KL, Smith CI, Schwarzenberg SJ, Jessurun J, Boldt MD, Parks EJ. Sources of fatty acids stored in liver and secreted via lipoproteins in patients with nonalcoholic fatty liver disease. J Clin Invest. 2005 ; 115(5) : 1343-51.   DOI
16 Koo SH. Nonalcoholic fatty liver disease: molecular mechanisms for the hepatic steatosis. Clin Mol Hepatol. 2013 ; 19(3) : 210-5.   DOI
17 Kim JK, Oh MS, Song TW. Oriental and western study on medication treatment of obesity. Daejeon Univ J Orient Med. 2002 ; 11(1) : 193-9.
18 Textbook Compilation Committee of National University of Korean Medicine, Korea. Herbology. Seoul : Younglimsa. 2018 : 218-9, 377-8, 458-9.
19 Jeong HJ. The effect of Galkun-Whanglyeon-Whanggum-Tang and Sopunghwalhyeol-tang in HUVEC on anti-inflammatory, anti-oxidant activity. Master's thesis. Naju: Dongshin University. 2020 : 1-67.
20 Cui X, Qian DW, Jiang S, Shang EX, Zhu ZH, Duan JA. Scutellariae Radix and Coptidis Rhizoma improve glucose and lipid metabolism in T2DM rats via regulation of the metabolic profiling and MAPK/PI3K/Akt signaling pathway. Int J Mol Sci. 2018 ; 19(11) : 3634.   DOI
21 Wang WB, Zhao LL, He ZY, Wu N, Li QX, Qiu XJ, et al. Metabolomics-based evidence of the hypoglycemic effect of Ge-Gen-Jiao-Tai-Wan in type 2 diabetic rats via UHPLC-QTOF/MS analysis. J Ethnopharmacol. 2018 ; 219 : 299-318.   DOI
22 Lyu Y, Lin L, Xie Y, Li D, Xiao M, Zhang Y, et al. Blood-glucose-lowering effect of Coptidis Rhizoma Extracts from different origins via gut microbiota modulation in db/db mice. Front Pharmacol. 2021 ; 12 : 684358.   DOI
23 Zou X, Huang W, Lu F, Fang K, Wang D, Zhao S, et al. The effects of Jiao-Tai-Wan on sleep, inflammation and insulin resistance in obestiy-resistant rats with chronic partial sleep deprivation. BMC Complement Altern Med. 2017 ; 17(1) : 165.   DOI
24 Di S, Han L, An X, Kong R, Gao Z, Yang Y, et al. In silico network pharmacology and in vivo analysis of berberine-related mechanisms against type 2 diabetes mellitus and its complications. J Ethnopharmacol. 2021 ; 276 : 114180.   DOI
25 Chen G, Yang X, Yang X, Li L, Luo J, Dong H, et al. Jia-Wei-Jiao-Tai-Wan ameliorates type 2 diabetes by improving β cell function and reducing insulin resistance in diabetic rats. BMC Complement Altern Med. 2017 ; 17(1) : 507.   DOI
26 Kim YJ, Kim JH, Noh YJ, Kim SJ, Hwang IH, Kim DK. Anti-oxidative effect of Salvia miltiorrhiza Bunge in Caenorhabditis elegans. Kor J Pharmacogn. 2018 ; 49(4) : 322-7.
27 Ran Q, Wang J, Wang L, Zeng HR, Yang XB, Huang QW. Rhizoma coptidis as a potential treatment agent for type 2 diabetes mellitus and the underlying mechanisms: a review. Front Pharmacol. 2019 ; 10 : 805.   DOI
28 Ahn SH, Kim KB. The effect of Coptidis Rhizoma and Glycyrrhiza Uralensis on lipid deposition with nonalcoholic fatty liver disease(NAFLD). J Pediatr Korean Med. 2019 ; 33(2) : 12-21.   DOI
29 Lee SE. Anti-obesity effect and action mechanism of Dansam-Samultang in C57BL/6 obese mice fed high fat diet. J Korea Soc Beauty Art. 2018 ; 19(2) : 113-25.   DOI
30 Kouame K, Peter AI, Akang EN, Moodley R, Naidu EC, Azu OO. Histological and biochemical effects of Cinnamomum cassia nanoparticles in kidneys of diabetic Sprague-Dawley rats. Bosn J Basic Med Sci. 2019 ; 19(2) : 138-45.
31 National Health Insurance Service. Health insurance obesity(E66) medical expenses [Internet]. Seoul: 2020 [cited 2021 Aug 4]. Available from: https://www.docdocdoc.co.kr/news/articleView.html?idxno=2003363.
32 Liu W, Chen X, Ge Y, Wang H, Phosat C, Li J, et al. Network pharmacology strategy for revealing the pharmacological mechanism of pharmacokinetic target components of San-Ye-Tang-Zhi-Qing formula for the treatment of type 2 diabetes mellitus. J Ethnopharmacol. 2020 ; 260 : 113044.   DOI
33 Korean Diabetes Association. Diabetes fact sheet in Korea 2018. Seoul : Korean Diabetes Association. 2018 : 19-20.
34 Heber D. Herbal preparations for obesity, are they useful? In: Bray GA, editor. Risks of obesity. Office management of obesity. Philadelphia : Elsevier Inc. 2004 : 107-26, 222-43.
35 Hwang MJ, Shin HD, Song MY. Literature review of herbal medicines on treatment of obesity since 2000 - mainly about Ephedra Herba. J Korean Med Obes Res. 2007 ; 7(1) : 39-54.
36 Uslu GA, Gelen V, Uslu H, Ozen H. Effects of Cinnamomum cassia extract on oxidative stress, immunreactivity of iNOS and impaired thoracic aortic reactivity induced by type II diabetes in rats. Braz J Pharm Sci. 2018 ; 54(3) : e17785.
37 Ma H, He K, Zhu J, Li X, Ye X. The anti-hyperglycemia effects of Rhizoma Coptidis alkaloids: a systematic review of modern pharmacological studies of the traditional herbal medicine. Fitoterapia. 2019 ; 134 : 210-20.   DOI
38 Zhou J, Pan J, Xiang Z, Wang Q, Tong Q, Fang J, et al. Xiaokeyinshui extract combination, a berberine- containing agent, exerts anti-diabetic and renal protective effects on rats in multi-target mechanisms. J Ethnopharmacol. 2020 ; 262 : 113098.   DOI