Journal of Environmental Science International (한국환경과학회지)

The Korean Environmental Sciences Society (한국환경과학회)
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Enhanced DPPH Radical Scavenging Activity of Lactobacillus plantarum K-21 Isolated from Kimchi and its Various Antioxidant Effects

김치유래 Lactobacillus plantarum K-21의 DPPH 라디칼 제거활성 증진 및 다양한 항산화 효과

  • Kim, Yerin (Department of Life Science and Environmental Biochemistry, Life and Industry Convergence Institute, Pusan National University) ;
  • Kim, Yedam (Department of Life Science and Environmental Biochemistry, Life and Industry Convergence Institute, Pusan National University) ;
  • Jeon, Chae-Min (Department of Life Science and Environmental Biochemistry, Life and Industry Convergence Institute, Pusan National University) ;
  • Park, Gyulim (Department of Life Science and Environmental Biochemistry, Life and Industry Convergence Institute, Pusan National University) ;
  • Lee, O-Mi (Avian Disease Division, Animal and Plant Quarantine Agency) ;
  • Son, Hong-Joo (Department of Life Science and Environmental Biochemistry, Life and Industry Convergence Institute, Pusan National University)
  • 김예린 (부산대학교 생명환경화학과 및 생명산업융합연구원) ;
  • 김예담 (부산대학교 생명환경화학과 및 생명산업융합연구원) ;
  • 전채민 (부산대학교 생명환경화학과 및 생명산업융합연구원) ;
  • 박규림 (부산대학교 생명환경화학과 및 생명산업융합연구원) ;
  • 이오미 (농림축산검역본부 조류세균과) ;
  • 손홍주 (부산대학교 생명환경화학과 및 생명산업융합연구원)
  • Received : 2022.07.05
  • Accepted : 2022.07.29
  • Published : 2022.08.31
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Abstract

Lactic Acid Bacteria (LAB) are among the representative probiotics that have been used for a long time in fermented food. Although there are many studies on detecting the radical scavenging activity of LAB, few studies have been conducted on the environmental factors that improve scavenging activity. This study investigated the environmental factors affecting the DPPH radical scavenging and various antioxidant activities of Kimchi-derived Lactobacillus plantarum K-21 with antihypertensive and radical scavenging activities. The optimal conditions for scavenging DPPH radicals were glucose 2%, bactopeptone 0.5%, Tween 80 0.05%, L-cysteine 0.05%, and an initial pH 6.5 at 35℃. Under optimal conditions, the DPPH radical scavenging activity was 94.8±2.2%, which was 1.5 times higher than that of the basic medium. In addition, L. plantarum K-21 had other antioxidant activities; ABTS radical scavenging (93.6±1.5%), hydroxyl radical scavenging (8.5±0.9%), metal chelating (65.9±0.5%), NO scavenging (53.1±19%), SOD-like (25.1±1.5%), and reducing power (11.7±1.4%) activities were detected. Therefore, L. plantarum K-21 may act not only as a starter for lactic acid-fermented foods with improved functionality but also as a drug for various diseases caused by oxidative stress.

Keywords

References

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