• Applied Biological Chemistry
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• v.9
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• pp.97-104
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• 1968

A survey of the free amino acids and free sugars in the juice of fourteen citrus varieties was made by means of paper chromatography and the pectin content in the rind was detected The results of the survey are summarized as follows. 1) Sixteen aminoacids detecteu in fourteen varieties under the condition of this study were distributed with. different frequency as follow. Proline, gamma-amino-butyric acid, alanine, valine, serine, glutamic acid, aspartic acid (in 14 varieties), lysine(12), leucine threonine(11), isoleucine(10), arginine(9), glycine(6) ${\beta}-alanine$(4) asparagine(3) unknown(2). 2) Proline, gamma-amino-butyric acid were mostly abundant in all varieties and alanine was next in the amount. 3) The varieties in the decreasing order of number of amino acids container were C. grandis madow C. leiocarpa (14 acids), C. sulcata, C. hassaku. native citron, Fortunella japonica(13) C. grandis heiko, C. Tamurana iyo, C. nobilis(12) C. reticulata C. junos(11) C. natsudaidai(10) C. miyakawa unshiu, C. ohali unshiu(9). 4) The varieties which appear to contain all essential amino acids(6 acids)detected were C. grandis madow C. grandis heiko, C. sulcata, C. Tamurana iyo, C. hassaku and native citron, and C. natsudaidai, C. unshiu were the least (1-3 acids). 5) Glucose fructose, sucrose and maltose were detected in all varieties. 6) The pectin content in the rind ranged from 8.64% F.W.(C. junos) up to 17.0% for C, grandis madow and the mean was $11.63{\pm}2.69%$.

• Ocean and Polar Research
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• v.33 no.1
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• pp.55-68
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• 2011

The distribution and inter-annual variation of nutrients (N, P, Si) and dissolved/particulate organic carbon were investigated in the equatorial thermocline ridge ($7^{\circ}{\sim}11.5^{\circ}N$, $131.5^{\circ}W$) of the northeast Pacific. From the Oceanic Nino Index and Multivariate ENSO Index provided by NOAA, normal condition was observed in July 2003 and August 2005 on the aspect of global climate/ocean change. However, La Ni$\~{n}$a and El Ni$\~{n}$o episodes occurred in July 2007 and August 2009, respectively. Thermocline ridge in the study area was located at $9^{\circ}N$ in July 2003, $8^{\circ}N$ in August 2005, $10^{\circ}N$ in July 2007, and $10.5^{\circ}N$ in August 2009 under the influence of global climate/ocean change and surface current system (North Equatorial Counter Current and North Equatorial Current) of the northeast Pacific. Maximum depth integrated values (DIV) of nutrients in the upper layer (0~100 m depth range) were shown in July 2007 (mean 21.12 gN/$m^2$, 4.27 gP/$m^2$, 33.72 gSi/$m^2$) and higher variability of DIV in the equatorial thermocline ridge was observed at $10^{\circ}N$ during the study periods. Also, maximum concentration of dissolved organic carbon (DOC) in the upper 50 m depth layer was observed in July 2007 (mean $107.48{\pm}14.58\;{\mu}M$), and particulate organic carbon (POC, mean $9.42{\pm}3.02\;{\mu}M$) was similar to that of DOC. Nutrient concentration in the surface layer increased with effect of upwelling phenomenon in the equatorial thermocline ridge and La Ni$\~{n}$a episode, which had formed in the central Pacific. This process also resulted in the increasing of organic carbon concentration (DOC and POC) in the surface layer. From these results, it is suggested that spatial and temporal variation of chemical and biological factors were generated by physical processes in the equatorial thermocline ridge.

• Journal of Bio-Environment Control
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• v.15 no.4
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• pp.335-339
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• 2006

Effects of deficit irrigation by different soil moisture-based water potential on total soluble solids and fruit yields in fresh tomato were investigated. Amount of irrigation was saved about 11%, 25% and 41% at -20 kPa,-30 kPa and -40 kPa treatment compared to the -15 kPa treatment as a standard practice, respectively. Deficit irrigation with -30 kPa and -40 kPa treatment significantly increased total soluble solids by 11% and 24% at first truss compared to the -15 kPa treatment, with no significant loss of yield, respectively. However, deficit irrigation with -30 kPa and -40 kPa treatment significantly increased total soluble solids at second and third truss, but resulted in substantial yield loss compared to the standard practice. Total fruit yields at -30 kPa and -40 kPa treatment was decreased by 16.5% and 25.1% compared to the -15 kPa treatment. However, marketable fruits based on fruit size (150$\sim$250g) was increased by 27.8% at -30 kPa treatment compared to the -15 kPa treatment.

• The Korean Journal of Pesticide Science
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• v.9 no.3
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• pp.231-236
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• 2005

This study was conducted to determine the amounts of pesticide residues after treatment of criterion dose with 4 pesticides(tolclofos-m, folpet, procymidone, and triflumizole) under cultivated period and to compare the biological half-life of pesticides with 6 kinetic models(first, zero and second order kinetics, power function, elovich and parabolic model) and to establish proposed field tolerance using biological half-lives. Recovery of 4 pesticides form strawberry was ranged from 85.1 to 105.5%. For all of 4 pesticides, dissipation rate was over 73% at 5 days after application. Among 6 kinetic models, first order kinetic model (FO) was best fit to describe the relationship between residual pattern of pesticides and time. Therefore, half-lives were calculated by FO for establishing the field tolerance. These results showed that half-life should be calculated by comparative best fit kinetic model and field tolerance can help to prevent unacceptable agricultural products from marketing. It is good for both consumers and farmers having safe agricultural products and financial benefits, respectively.

• Korean Journal of Medicinal Crop Science
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• v.10 no.2
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• pp.132-138
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• 2002

This study was performed to determine the antimutagenic and cytotoxic effect of ,Kalopanacis cortex endothelium. Methanol extract was used on Salmonella typhimurium TA98, TA100 and three cancer cell lines. In the Ames test, methanol extract of Kalopanacis cortex endothelium alone did not exhibit any mutagenicity but showed substantial inhibitory effects against mutation induced by 4-nitroquinoline-l-oxide(4NQO), N'-methyl- N'-nitro-N-nitrosoguanidine(MNNG) and benzo(a)pyrene(B(a)P). The methanol extract of Kalopanacis cortex endothelium showed approximately 79.29% and 75.38% inhibitory effect on the mutagenesis induced by 4NQO and B(a)P, respectively, against TA98 strain. Whereas 79.49%, 89.3% and 68.85% inhibitions were observed on the mutagenesis induced by 4NQO, MNNG and B(a)P against TA100 strain. Methanol extracts from Kalopanacis cortex endothelium showed high antimutagenic effects against 4NQO, MNNG and B(a)P. The anticancer effects of methanol extract from Kalopanacis cortex endothelium against human lung carcinoma(A549), human breast adenocarcinoma (MCF-7) and human hepatocellular carcinoma(Hep3B) were investigated. The treatment of 0.5mg/ml Kalopanacis cortex endothelium methanol extracts had the highest cytotoxicity against A549(81.54%), followed by MCF-7(81.92%) and Hep3B (78.57%). In contrast 0.5mg/ml treatment of methanol extracts from Kalopanacis cortex endothelium had only $4{\sim}25%$ cytotoxicity on normal human liver cell(293).

• Allergy, Asthma & Immunology Research
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• v.10 no.6
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• pp.648-661
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• 2018

Purpose: Pollen-food allergy syndrome (PFAS) is an immunoglobulin E (IgE)-mediated allergy in pollinosis patients caused by raw fruits and vegetables and is the most common food allergy in adults. However, there has been no nationwide study on PFAS in Korea. In this study, we investigated the prevalence and clinical characteristics of PFAS in Korea. Methods: Twenty-two investigators participated in this study, in which patients with allergic rhinoconjunctivitis and/or bronchial asthma with pollen allergy were enrolled. The questionnaires included demographic characteristics, a list of fruits and vegetables, and clinical manifestations of food allergy. Pollen allergy was diagnosed by skin prick test and/or measurement of the serum level of specific IgE. Results: A total of 648 pollinosis patients were enrolled. The prevalence of PFAS was 41.7% (n = 270). PFAS patients exhibited cutaneous (43.0%), respiratory (20.0%), cardiovascular (3.7%) or neurologic symptoms (4.8%) in addition to oropharyngeal symptoms. Anaphylaxis was noted in 8.9% of the PFAS patients. Seventy types of foods were linked to PFAS; e.g., peach (48.5%), apple (46.7%), kiwi (30.4%), peanut (17.4%), plum (16.3%), chestnut (14.8%), pineapple (13.7%), walnut (14.1%), Korean melon (12.6%), tomato (11.9%), melon (11.5%) and apricot (10.7%). Korean foods such as taro/taro stem (8.9%), ginseong (8.2%), perilla leaf (4.4%), bellflower root (4.4%), crown daisy (3.0%), deodeok (3.3%), kudzu root (3.0%) and lotus root (2.6%) were also linked to PFAS. Conclusions: This was the first nationwide study of PFAS in Korea. The prevalence of PFAS was 41.7%, and 8.9% of the PFAS patients had anaphylaxis. These results will provide clinically useful information to physicians.

• Korean Journal of Food Science and Technology
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• v.33 no.5
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• pp.619-625
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• 2001

We determined whether the supplementation of Polygonatum Odoratum (Mill) Druce (POD) extract had a good effect on insulin resistance in peripheral tissues of 90% pancreatectomized (Px) and sham-operated (Sham) male Sprague Dawley rats. Px and Sham rats were divided into two groups; one group daily consumed 0.3 g of POD extracts per 1 ㎏ body weight for two months, and the other group had a placebo. All rats freely consumed a 40% fat diet. At the end of the experiment, a euglycemic hyperinsulinemic (EH) clamp was performed in a fasting, awake, and unstressed state to determine insulin resistance. At EH clamp, body weights were higher in Sham rats than Px rats, and serum glucose levels of baseline were affected by diabetic status and POD administration. Serum insulin concentrations were higher in Sham rats than Px rats, and POD administration decreased them in Sham rats compared to P. Glucose disposal rates in peripheral tissues increased with POD in both Px (n=10) and Sham (n=10) rats. But glycogen deposits in soleus muscle increased with POD administration in Px and Sham rats, and total glycogen synthase activity and fraction velocity were higher in POD groups. Triglyceride contents in quadriceps muscles decreased with POD in Px rats. In conclusions, POD improves insulin resistance by enhancing glucose utilization with increasing glycogen deposit and decreasing triglyceride contents in muscles.

• Korean Journal of Environmental Agriculture
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• v.36 no.3
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• pp.169-174
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• 2017

BACKGROUND:Kiwifruit growers build their vineyards using many windbreaks to protect their kiwifruit vines from defoliation injury by strong winds such as typhoon. In this study, we have compared fruit quality, budbreak rate and floral bud as affected by windbreaks. And also we surveyed several microclimate indices of kiwifruit orchard depending on the covering materials of arch-type windbreaks. METHODS AND RESULTS: Five different windbreak materials including polyethylene film (PE), blue- and white-colored nets were tested in pipe-framed archtype kiwifruit vineyards as the covering materials. Photosynthetically active radiation (PAR), annual mean temperature (AMT) and chill unit (CU) as well as fruit quality were compared among the covering materials. In all treatments, annual PAR was more than $400{\mu}mol\;m^{-2}s^{-1}$, in which kiwifruit leaf could reach its maximum photosynthesis, since the leaves were emerged. Annual mean temperature was greater in 0.1 mm-PE covering as much as $1-2^{\circ}C$ than other windbreaks. In CU calculated by three different models, all windbreaks showed more than 1400 CU that is fully fulfilled CU for kiwifruit rest completion. There were no difference in budbreak rate among the covering materials. Fruit weight was heavier in 0.1 mm-PE and white-net (4 mm) than other windbreaks. CONCLUSION: Regardless of the windbreak materials, the PAR quantity was enough for kiwifruit photosynthesis. And CU for kiwifruit rest completion was fully achieved in all treatments. However, with respect to fruit weight, quantity of PAR, and AMT, etc., It is highly recommended for kiwifruit growers to choose 0.1 mm-PE and white-net (4 mm) as for their windbreaks materials.

• Restorative Dentistry and Endodontics
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• v.33 no.2
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• pp.148-161
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• 2008

The purpose of this study was to evaluate the effect of chlorhexidine (CHX) on microtensile bond strength (${\mu}TBS$) of dentin bonding systems. Dentin collagenolytic and gelatinolytic activities can be suppressed by protease inhibitors, indicating that MMPs (Matrix metalloproteinases) inhibition could be beneficial in the preservation of hybrid layers. Chlorhexidine (CHX) is known as an inhibitor of MMPs activity in vitro. The experiment was proceeded as follows: At first, flat occlusal surfaces were prepared on mid-coronal dentin of extracted third molars. GI (Glass Ionomer) group was treated with dentin conditioner, and then, applied with 2 % CHX. Both SM (Scotchbond Multipurpose) and SB (Single Bond) group were applied with CHX after acid-etched with 37% phosphoric acid. TS (Clearfil Tri-S) group was applied with CHX, and then, with adhesives. Hybrid composite Z-250 and resin-modified glass ionomer Fuji-II LC was built up on experimental dentin surfaces. Half of them were subjected to 10,000 thermocycle, while the others were tested immediately. With the resulting data, statistically two-way ANOVA was performed to assess the ${\mu}TBS$ before and after thermo cycling and the effect of CHX. All statistical tests were carried out at the 95 % level of confidence. The failure mode of the testing samples was observed under a scanning electron microscopy (SEM). Within limited results, the results of this study were as follows; 1. In all experimental groups applied with 2 % chlorhexidine, the microtensile bond strength increased, and thermo cycling decreased the micro tensile bond strength (P > 0.05). 2. Compared to the thermocycling groups without chlorhexidine, those with both thermocycling and chlorhexidine showed higher microtensile bond strength, and there was significant difference especially in GI and TS groups. 3. SEM analysis of failure mode distribution revealed the adhesive failure at hybrid layer in most of the specimen. and the shift of the failure site from bottom to top of the hybrid layer with chlorhexidine groups. 2 % chlorhexidine application after acid-etching proved to preserve the durability of the hybrid layer and microtensile bond strength of dentin bonding systems.

• Journal of Mushroom
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• v.7 no.3
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• pp.130-134
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• 2009

To develop new white variety of Oyster mushroom, all white varieties which have been collected and kept in the lab were revived and screened their cultural characteristics. 84 intra-specific Oyster mushroom hybrids between the white-colored mutants Suhan and Wonhyeong were developed using hyphal anastomosis technique in 2007. The Po2007-63 ($2842-7{\times}0205-7$) was shown the best cultural characteristics, selected to be a new variety and named as 'Goni'. The new commercial strain, 'Goni' has white pilei and grows well under spring and autumn conditions in Korea. The fruiting bodies of 'Goni' are of an excellent quality in that not only the stipe is thick and long but also the pileus is small and hard. The optimum temperatures for mycelial growth and fruiting body development were $25-30^{\circ}C$ and $10-16^{\circ}C$, respectively. Time period required for the initiation of the first fruiting body is about 3 to 5 days depending on the temperatures. The shape of fruiting body is thin funnel shape. Fruiting body production per bottle was about $91{\pm}13$ g which is almost 97% quantity compared to that of other variety 'Miso'. Relatively low temperature incubation ($11^{\circ}C$) resulted in the development of better quality of 'Goni' mushrooms. When two different media including potato dextrose medium and mushroom complete medium were compared, the growth of mushroom were much faster in mushroom complete medium at $20-25^{\circ}C$, but not at $25^{\circ}C$. Similar results were observed with other variety 'Miso'. Analysis of the genetic characteristics of the new commercial strain 'Goni' showed a major DNA profile as that of the parental Suhan when primer URP 1 was used, but different to 'Miso' that was used as a control. When screens were performed with primer URP 2, DNA patterns were similar both to that of the parents and 'Miso'. This new variety of the white Oyster mushroom has a clean and fresh image that corresponds well to "health food". We therefore expect that this new strain will satisfy the consumers demand for variety and excellent mushrooms.