• Title/Summary/Keyword: ${\mu}$-Analysis

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Quantitative Analysis of Lignans in the Fruits of Acanthopanax Species by HPLC

  • Kim, Hye-Min;Kim, Ju-Sun;Lee, Sang-Hyun;Lee, Sung-Jin;Lee, Gung-Pyo;Kang, Sam-Sik;Cho, Seon-Haeng;Cheoi, Dae-Sung
    • Food Science and Biotechnology
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    • v.15 no.5
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    • pp.778-780
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    • 2006
  • High performance liquid chromatography (HPLC) was used for the analysis of the lignans eleutheroside B and eleutheroside E in the fruits of Acanthopanax species. A reverse-phase system using a gradient of $H_2O$ and acetonitrile as the mobile phase was developed and detection was at 210 nm. The analysis was successfully carried out within 20 min. The content of eleutheroside B and eleutheroside E in Acanthopanax species was measured in the fruits of A. senticosus (0.58 and $1.66\;{\mu}g/mg$, respectively), A. sessiliflorus (1.15 and $8.49\;{\mu}g/mg$, respectively), A. koreanum (2.16 and $1.80\;{\mu}g/mg$, respectively), and A. divaricatus (1.06 and $7.08\;{\mu}g/mg$, respectively).

Optimization of Gene Transfection Using Fluorescence-Activated Cell Sorter(FACS) Analysis of Green Fluorescent Protein(GFP) (Green Fluorescent Protein(GFP)의 Fluorescence-Activated Cell Sorter(FACS) 분석을 통한 유전자 이입의 최적화)

  • 김태경;박민태;이균민
    • KSBB Journal
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    • v.14 no.3
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    • pp.377-379
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    • 1999
  • In order to improve the transfection efficiency of CHO/dhfr- cells using cationic lipid, optimal concentrations of the cationic lipid($LipofectAmine^{TM}$) and DNA(pEGFP-C1) need to be determined. The use of green fluorescent protein(GFP) gene as a reporter gene facilitated the quantification of transfection efficiency. The green fluorescence intensity of each cell transfected at various lipid-DNA concentrations was measured using fluorescence-activated cell sorter(FACS) analysis. A combination of $2.0{\mu}L$ cationic lipid and 0.4{$\mu}g$ DNA in a well resulted in the highest trasfection efficiency. Taken together, the method using FACS analysis of GFP is simple and fast, facilitating the optimization of transfection.

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IR Signal Analysis and Band Selection for Two-color Seeker (Two-color 탐색기를 위한 적외선 신호 분석 및 대역 선정)

  • 오정수;서동선;최종수
    • The Journal of Korean Institute of Communications and Information Sciences
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    • v.25 no.1B
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    • pp.112-119
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    • 2000
  • A two-color infrared (IR) seeker employs two detection-bands for efficient target tracking under countermeasures and background noises. This paper describes a study on the detection-band selections for the two-color seeker. To select the detection-bands, we analysis infrared radiation characteristics of a target, a flare, and background noises and then other characteristics closely related with the received power, such as the atmospheric transmission, acquisition range, background noise effect, and signal-to-noises. The analysis shows that the pertinent bands of two-color IR seeker are 3 um~5 um for target detection and 1.5 um~l.7 um for counter-countermeasures. The results of our study will play a significant role in the further study of two-color seekers.

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The Characterizing Analysis of a Buried-Channel MOSFET based on the 3-D Numerical Simulation

  • Kim, Man-Ho;Kim, Jong-Soo
    • Journal of Electrical Engineering and Technology
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    • v.2 no.2
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    • pp.267-273
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    • 2007
  • A depletion-mode MOSFET has been analyzed to evaluate its electrical behavior using a novel 3-D numerical simulation package. The characterizing analysis of the BC MOSFET was performed through short-channel narrow-channel and small-geometry effects that are investigated, in detail, in terms of the threshold voltage. The DIBL effect becomes significant for a short-channel device with a channel length of $<\;3({\mu}m)$. For narrow-channel devices the variation of the threshold voltage was sharp for $<4({\mu}m)$ due to the strong narrow-channel effect. In the case of small-geometry devices, the shift of the threshold voltage was less sensitive due to the combination of the DIBL and substrate bias effects, as compared with that observed from the short-channel and narrow-channel devices. The characterizing analysis of the narrow-channel and small-geometry devices, especially with channel width of $<\;4({\mu}m)$ and channel area of $<\;4{\times}4({\mu}m^2)$ respectively, can be accurately performed only from a 3-D numerical simulation due to their sharp variations in threshold voltages.

Analysis and Design of Si3N4 Rib-optical Waveguides for Evanescent-wave Integrated-optical Biosensors (소산파 집적광학 바이오센서에 적합한 Si3N4 립-광도파로 해석 및 설계에 관한 연구)

  • Jung, Hongsik
    • Korean Journal of Optics and Photonics
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    • v.30 no.1
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    • pp.15-22
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    • 2019
  • $Si_3N_4$ rib-optical waveguides for evanescent-wave integrated-optical biosensors were analytically interpreted, to derive the single-mode propagation conditions. The integrated-optical biosensor structure based on two-mode interference was proposed, and the rib width and thickness and core thickness for a single-mode and two-mode waveguide (sensing region) were proposed to be $3{\mu}m$, 2 nm, and 150 nm and $3{\mu}m$, 20 nm, and 340 nm respectively. The optical characteristics of each guided-wave mode were investigated utilizing the film mode-matching (FMM) analysis.

3D micro-CT analysis of void formations and push-out bonding strength of resin cements used for fiber post cementation

  • Uzun, Ismail Hakki;Malkoc, Meral Arslan;Keles, Ali;Ogreten, Ayse Tuba
    • The Journal of Advanced Prosthodontics
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    • v.8 no.2
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    • pp.101-109
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    • 2016
  • PURPOSE. To investigate the void parameters within the resin cements used for fiber post cementation by micro-CT (${\mu}CT$) and regional push-out bonding strength. MATERIALS AND METHODS. Twenty-one, single and round shaped roots were enlarged with a low-speed drill following by endodontic treatment. The roots were divided into three groups (n=7) and fiber posts were cemented with Maxcem Elite, Multilink N and Superbond C&B resin cements. Specimens were scanned using ${\mu}CT$ scanner at resolution of $13.7{\mu}m$. The number, area, and volume of voids between dentin and post were evaluated. A method of analysis based on the post segmentation was used, and coronal, middle and apical thirds considered separately. After the ${\mu}CT$ analysis, roots were embedded in epoxy resin and sectioned into 2 mm thick slices (63 sections in total). Push-out testing was performed with universal testing device at 0.5 mm/min cross-head speed. Data were analyzed with Kruskal-Wallis and Mann-Whitney U tests (${\alpha}=.05$). RESULTS. Overall, significant differences between the resin cements and the post level were observed in the void number, area, and volume (P<.05). Super-Bond C&B showed the most void formation ($44.86{\pm}22.71$). Multilink N showed the least void surface ($3.51{\pm}2.24mm^2$) and volume ($0.01{\pm}0.01mm^3$). Regional push-out bond strength of the cements was not different (P>.05). CONCLUSION. ${\mu}CT$ proved to be a powerful non-destructive 3D analysis tool for visualizing the void parameters. Multilink N had the lowest void parameters. When efficiency of all cements was evaluated, direct relationship between the post region and push-out bonding strength was not observed.

Competitive Extraction and Trace Analysis of BTEX and MTBE by Solid-Phase Microextraction (SPME) (고체상미량추출법을 이용한 BTEX와 MTBE의 경쟁적 추출효과 및 미량분석에 관한 연구)

  • An, Sang-Woo;Chun, Suk-Young;Lee, Si-Jin;Park, Jae-Woo;Chang, Soon-Woong
    • Journal of Korean Society on Water Environment
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    • v.26 no.4
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    • pp.622-628
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    • 2010
  • In this study, Solid-phase microextraction (SPME) with GC/FID was studied as a possible alternative to liquid-liquid extraction for the analysis of BTEX and MTBE. Experimental parameters affecting the SPME process (such as kind of fibers, adsorption time, desorption time, volume ratio of sample to headspace, salt addition, and magnetic stirring) were optimized. Experimental parameters such as CAR/PDMS, adsorption time of 20 min, desorption time of 5 min at $250^{\circ}C$, headspace volume of 50 mL, sodium chloride (NaCl) concentration of 25% combined with magnetic stirring were selected in optimal experimental conditions for analysis of BTEX and MTBE. The general affinity of analytes to CAR/PDMS fiber was high in the order p-Xylene>Toluene>Ethylbenzene>MTBE>Benzene. The linearity of $R^2$ for BTEX and MTBE was from 0.970 to 0.999 when analyte concentration ranges from $30{\mu}g/L$ to $500{\mu}g/L$, respectively. The relative standard deviation (% RSD) were from 2.5% to 3.2% for concentration of $100{\mu}g/L$ (n=5), respectively. Finally, the limited of detection (LOD) observed in our study for BTEX and MTBE were from $7.5{\mu}g/L$ to $15{\mu}g/L$, respectively.

Radial Type Satellite Attitude Controller Design using LMI Method and Robustness Analysis (LMI 방법을 이용한 방사형 인공위성 제어로직 설계 및 강건성 분석)

  • Rhee, Seung-Wu
    • Journal of the Korean Society for Aeronautical & Space Sciences
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    • v.43 no.11
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    • pp.998-1007
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    • 2015
  • The $H_{\infty}$ control theory using LMI method is applied to design an attitude controller of radial type satellite that has strongly coupled channels due to the large product of inertia. It is observed that the cross-over frequency of open-loop with $H_{\infty}$ controller is lower than that of open-loop without controller, which is not typical phenomenon in an optimal control design result: it is interpreted that due to a large product of inertia, there is certain limit in increasing agility of satellite by just tuning weighting function. ${\mu}$-analysis is performed to verify the stability and performance robustness with the assumption of +/-5% MOI variation. ${\mu}$-analysis result shows that the variation of principal MOI degrades the stability and performance robustness more than the variation of POI does.

Theoretical modeling and analysis of thulium doped fluoride fiber amplifier at 1.47 $\mu\textrm{m}$ band amplification (툴륨이 첨가된 플루오르 계열의 광섬유 증폭기의 1.47$\mu\textrm{m}$ 증폭 대역에 대한 모델링과 분석에 대한 연구)

  • 이원재;민범기;박재형;박남규
    • Korean Journal of Optics and Photonics
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    • v.11 no.3
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    • pp.171-178
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    • 2000
  • We present a numerical model which enables to analyze $1.47mu$m amplification band characteristics for thulium doped fluoride fiber amplifiers. We focused on upconversion pumping scheme, thus many transitions affecting $1.47mu$m band amplification was considered simultaneously. Backward propagating waves and transverse mode were also considered in the model. The parameters for modeling were then acquired using published experimental data and related theories such as Judd-Ofelt theory for radiative transition, empirical energy gap law for non-radiative transition, and McCumber relations for cross-sections. The simulation showed well-matched results with experiment and internal dynamics. amics.

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Process Capability Analysis by the Experiment of Chips (Chips 실험에 의한 공정능력의 해석)

  • 송서일;황의철
    • Journal of Korean Society of Industrial and Systems Engineering
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    • v.10 no.16
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    • pp.63-73
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    • 1987
  • Process capability is reflected on four major factors such as materials, equipments. skill of operators, and methods. The status of the process is typically represented by the mean value 〔$\mu$〕as a central tendancy, and the variance 〔$\sigma$$^2$〕 as a magnitude of dispersion. This paper analyzes the process capability by the experiment of chips is accounted on a population from the process. So, this paper analyzes the next four cases : (1) When the process distribution is changed from N[$\mu$$_1$, $\sigma$$^2$〕to N〔$\mu$$_2$, $\sigma$$^2$〕. (2) When the process distribution is changed from N[$\mu$, $\sigma$$_1$$^2$] to N[$\mu$, $\sigma$$_2$$^2$]. (3) When the population is compounded by the different two distributions of N〔$\mu$$_1$, $\sigma$$^2$〕and N〔$\mu$$_2$, $\sigma$$^2$〕. (4) When the population is compounded by the different two distributions of N〔$\mu$, $\sigma$$_1$$^2$〕 and N[$\mu$$\sigma$$_2$$^2$].

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