• 제목/요약/키워드: ${\beta}$-lactamase

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Sulbactam 유도체의 베타락타마제 효소억제력과 베타락탐항생제 병용시 활성비교 ($\beta$-Lactamase Inhibitory Activity and Comparative Activity of Sulbactam Derivatives Combined with $\beta$-Lactam Antibiotics)

  • 임채욱;박희석;김용현;임철부
    • 약학회지
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    • 제46권6호
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    • pp.387-391
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    • 2002
  • In vitro $\beta$-lactamase inhibitory activity of 6-exomethylene sulbactam compounds (CH-120, 130, 140, 145, 150, 155) was compared with clavulanic acid, sulbactam and tazobactam. The inhibitory activity of CH-140 was stronger than sulbactam and clavulanic acid against Type II, III, IV, TEM enzymes and stronger than tazobactam against Type IV enzyme. The inhibitory activity of CH-145 was stronger than sulbactam and clavulanic acid against Type I, II, III, IV, TEM enzymes and stronger than tazobactam against Type III, IV enzymes. The in vitro antimicrobial activity of CH-140 and CH-145 combined with piperacillin and ceftriaxone was compared with the sulbactam and tazobactam against $\beta$-lactamase producing 31 strains. But, synergistic activity of CH-140 and CH-145 was inferior to tazobactam.

국내 가금유래 병원성 대장균의 extended-spectrum β-lactamase(ESBL) 특성 조사 (Survey of extended-spectrum β-Lactamase (ESBL) in pathogenic Escherichia coli isolated from poultry in Korea)

  • 성명숙;김진현;조재근;설성용;김기석
    • 대한수의학회지
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    • 제48권3호
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    • pp.259-265
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    • 2008
  • This study was conducted to investigate incidence of extended-spectrum ${\beta}$-lactamase (ESBL) producing strains and characteristics of ESBL gene in pathogenic Escherichia coli isolated from poultry during the period from April 2003 to December 2005 in Korea. Among 203 isolates, 4 isolates (3 from broilers and 1 from layer) were confirmed as ESBL producing strains by double disk synergy test, polymerase chain reaction and sequencing for ${\beta}$-lactamase genes. $bla_{CTX-M-15}$ and $bla_{CMY-2}$ were detected in these 4 isolates and were transferred to recipient by conjugation, respectively. Also, these ESBL producing strains were associated with multiple drug resistance. In conclusion, these results exhibit incidence of CTX-M and CMY-2 ${\beta}$-lactamase in pathogenic E coli from poultry in Korea, and clinically important meaning in human. And they also suggest the needs for rapid and broad surveillance to monitor ESBL genes and R plasmid transferring resistant gene in poultry.

Detection and Identification of $\beta$-lactamase, Enterotoxin and Other Exotoxins Genes of Staphylococcus aureus by PCR

  • Yoon, Y.H.;Kim, K.I.
    • Asian-Australasian Journal of Animal Sciences
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    • 제16권3호
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    • pp.425-429
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    • 2003
  • Staphylococcus aureus is a major pathogen for cattle, causing various forms of subclinical and clinical mastitis and could be a causative agent of food poisoning, it produces various superantigenic exotoxins which have a great public health significance. A total of 72 S. aureus clinical isolates from dairy farms located in Kyunggi Province Korea were examined for the species identification by biochemical method, and for the detection of $\beta$-lactamase, enterotoxin and other exotoxins genes by PCR. The results of species identification by biochemical method agreed with those of PCR done with species specific primer STA-AU. $\beta$-lactamase is an enzyme closely associated with the resistance to antibiotic penicillin, which is an important means of treatment of mastitis, all the isolates were positive for the presence of genes encoding $\beta$-lactamase, which were reproduced in penicillin susceptibility disc assay. Six types of toxin genes, Staphylococcal enterotoxin (SE)A, SEB, SEC, SEE, toxic shock syndrome toxin (TSST-1) and exfoliative toxin A (ET A) were detected in 72 isolates by PCR associated genotypic method in this study, none of the isolates carried the genes for enterotoxin D (SED) and exfoliative toxin B (ETB). The occurrence rate of exotoxin genes rated as 12.5%, and the precision of the PCR identification results has been confirmed using the reference strains.

방선균 일주에서 포자형성과 호알칼리성 단백질 분해효소의 생합성과의 관계성 (Relationship between Sporulation and Synthesis of Alkaline Protease in Streptomyces sp.)

  • 정병철;신현승;이계준
    • 미생물학회지
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    • 제26권4호
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    • pp.355-361
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    • 1988
  • 성장속도가 빠르고포지형성이 우수한 방사균 일주를 토양에서 분리한 뒤 분리균의 특성을 조사한 결과 세포외 단백질 분해 효소가 중성과 알카리성의 두 종류가 생성되었으며 $\beta$-lactamase도 생성함을 알았다. 이 균주를 acriflarin 또는 NTG로서 처리하여 얻은 변이주는 포자의 형성, $\beta$-lactamase의 생성 및 protease의 생합성 능력이 소실 또는 크게 저하되었다. 일단계의 변이주 취득에서 동시에 형질의 변화가 다양하게 나타난 원인을 규명한 결과 호알카리성 protease의 생합성이 크게 저하되었음을 알았다. 따라서 방선균에서 포자형성과 호알카리성 protease의 활성이 일정한 연관성이 있을 것으로 판단되었다.

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Neisseria gonorrhoeae분리균주의 특성 (Characterization of Isolated strains of Neisseria gonorrhoea)

  • 최영기;정윤섭;김수영;이형환
    • 미생물학회지
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    • 제24권1호
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    • pp.73-78
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    • 1986
  • N. gonorrhoeae 81균주를 320개의 가검물에서 분리동정한 후에 증식에 대한 VCN의 영향과 lsovitalex의 양에 따른 영향, 당분해능에 관한 실험과 ${\beta}-lactamase$생산균의 검출 및 plasmid분리에 대하여 연구하였다. 전체 320개의 가검물중에서 81균주의 N.gonorrhoeae가 분리동정되었고, chocolate agar에서는 81균주 중 72균주가,1% Isovitalex와 1 % VCN을 함유한 Thayer- Martin배지에서는 80균주가, 2 % Isovitalex와 1 % VCN이 든 Thayer-Martin 배지는 55균주가 N. gonorrhoeae의 특성을 나타냈다. Coagglutination시험에 양성인 81균주 중에서 67균주는 48시간 배양후에,10균주는 72시간 배양후에 glucose 배지에서 산을 생산하였으나, 나머지 4균주는 96시간 배양후에도 산을 생산하지 않았다. 인천지역 특수직업여성에서 분리된 81균주의 N.gonorrhoeae 중에서 41 균주가 ${\beta}-lactamase$를 생산하였고, ${\beta}-lactamase$ 생산 균주인 N. gonorrhoeae PL-118은 2.6. 4.5 및 24.5Mdal의 plasmid DNA를 포함하였다.

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Synthesis and $\beta$-lactamase inhibitory activity of 6-exomethylene penamsulfone derivatives-III (In vitro $\beta$-lactamase inhibitory activity and comparative susceptibility test of 6-exomethylene penamsulfone derivatives)

  • Park, Hee-Suk;Yoon, Sang-Bae;Chaeuk Im;Yim, Chul-Bu
    • 한국응용약물학회:학술대회논문집
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    • 한국응용약물학회 1997년도 춘계학술대회
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    • pp.72-72
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    • 1997
  • 합성한 5종의 6-Exomethylene penamsulfone 유도체의 Type I, Type II, Type III, TypeIV, TEM 효소에 대한 $\beta$-lactamase저해효과를 spectrophotometric assay방법으로 측정하였다. 또한 여기서 우수한 효소억제활성을 보여준 3종의 화합물을 가지고 In vitro antibacterial activity를 Agar dilution method법으로 27종의 $\beta$-lactamase생성균주에 대하여, Sulbactam, Ampicillin 및 Cefoperazone과 병용투여하여 MIC를 측정하였다.

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${\beta}-Lactamase$ 억제작용이 기대되는 7-Arylidene Cephalosporanate 유도체의 합성 (Synthesis of 7-Arylidene Cephalosporanates for ${\beta}-lactamase$ Inhibitor)

  • 이종민;임철부;임채욱
    • 약학회지
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    • 제52권4호
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    • pp.311-315
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    • 2008
  • The synthesis of 7-arylidene cephalosporanates for ${\beta}-lactamase$ inhibitor was described. The reactions of substituted benzyl halides $[1]{\sim}[3]$ with triphenylphosphine gave triphenylphcsphonium chlorides $[4]{\sim}[6]$. These phosphonium salts were treated with n-butyllithium to give ylides, which were reated with 7-oxocephalosporanate [7] by Wittig reaction to afford the 7-exomethylene cephalosporanates $[8]{\sim}[10]$. These cephalosporanates were oxidized to cephalosporanate sulfones $[11]{\sim}[13]$ with mCPBA. The deprotection of benzhydryl cephalosporanate $[8]{\sim}[13]$ with $AlCl_3$ and $NaHCO_{3}$ gave sodium salts of 7-arylidene cephalosporanates $[14]{\sim}[19]$.

Multidrug-Resistant Providencia Isolates Carrying $bla_{PER-1},\;bla_{VIM-2}$, and armA

  • Lee, Hee-Woo;Kang, Hee-Young;Shin, Kyeong-Seob;Kim, Jung-Min
    • Journal of Microbiology
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    • 제45권3호
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    • pp.272-274
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    • 2007
  • During May to July 2004, three strains of Providencia spp. with multidrug-resistance (MDR) were isolated from urinary specimen of three patients hospitalized with a same hospital room. By PCR analysis, all three strains have been found to carry both VIM-2 type $metallo-{\beta}-lactamase$ gene and PER-1 type extended spectrum ${\beta}-lactamase$ gene. One out of three strains carried additional resistance gene, armA, 16S rRNA methylase gene responsible for high level resistance to aminoglycosides. To our knowledge, this is the first report on the identification of Providencia spp. simultaneously carrying $bla_{VIM-2},\;bla_{PER-1}$, and armA genes.

부산시내 종합병원의 임상 검체에서 분리된 Extended -Spectrum $\beta$-Lactamase 생성 Klebsiella pneumoniae의 형별 분류 (Extended-Spectrum $\beta$-Lactamase (ESBL) Typing of Klebsiella pneumoniae Isolated from Clinical Specimen in Pusan)

  • 김윤태;이훈구
    • 미생물학회지
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    • 제36권3호
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    • pp.221-227
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    • 2000
  • 본 연구는 등전점과 PCR 생성물을 통하여 광범위 $\beta$-Lactamase(ESBL) 를 생성하는 Klebsiella pneumoniae의 ESBL 유형을 결정하였다. 부산시내 소재 3개 종합병원의 임상검체로부터 20균주의 ESBL 을생성하는 Klebsiella pneumoniae를 분리하였다. 제 3세대 cephalosporin인 cefotaime, cef-tazidime, ceftriaxone으로 double disk synergy test를 시행하여 ESBL 생성균주를 확인하였다. 등전점 및 PCR결과 TEM 형 (pI 5.2~6.0, 1080 bp), TEM+SHV 혼합형(pI 5.2~6.0 및 pI 7.0~7.4 로 1080 bp, 599bp), SHV형 (pI 7.0~ 7.4, 599 bp) , 그리고 PCR결과는 SHV형으로 나타났으나 등전점을 나타내지 않는 것과 등전점 및 PCR 결과 어느 쪽에도 속하지 않는 non TEM non SHV 형 등 5개군으로 나뉘어 졌다.

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Investigation of Klebsiella pneumoniae Isolates Producing SHV-12 and SHV-11 ${\beta}$-Lactamases in Korean Hospitals

  • Lee, Kyeong-Min;Yoo, Jae-Il;Yoo, Yong-Sun;Yoo, Jung-Sik;Chung, Gyung-Tae;Ahn, Tae-In;Lee, Yeong-Seon
    • Journal of Microbiology and Biotechnology
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    • 제19권9호
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    • pp.1065-1069
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    • 2009
  • Of 143 clinical isolates of Klebsiella pneumoniae collected from Korean non-tertiary hospitals, 24 (16.8%) showed an extended-spectrum ${\beta}$-lactamase-positive phenotype. PCR and sequence analysis revealed the presence of TEM-116 (n=13), CTX-M-3 (n=5), CTX-M-14 (n=2), CTX-M-15 (n=3), and SHV-12 (n=16). Each of the 24 isolates encoded more than one ${\beta}$-lactamase, and seven isolates (29%) harbored two different SHV-type ${\beta}$-lactamase genes ($bla_{SHV-11}$ and $bla_{SHV-12}$) bounded by insertion sequence IS26 in a single transferable plasmid.