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Investigation of Klebsiella pneumoniae Isolates Producing SHV-12 and SHV-11 ${\beta}$-Lactamases in Korean Hospitals  

Lee, Kyeong-Min (Division of Antimicrobial Resistance, National Institute of Health)
Yoo, Jae-Il (Division of Antimicrobial Resistance, National Institute of Health)
Yoo, Yong-Sun (Division of Antimicrobial Resistance, National Institute of Health)
Yoo, Jung-Sik (Division of Antimicrobial Resistance, National Institute of Health)
Chung, Gyung-Tae (Division of Antimicrobial Resistance, National Institute of Health)
Ahn, Tae-In (School of Biological Sciences, Seoul National University)
Lee, Yeong-Seon (Division of Antimicrobial Resistance, National Institute of Health)
Publication Information
Journal of Microbiology and Biotechnology / v.19, no.9, 2009 , pp. 1065-1069 More about this Journal
Abstract
Of 143 clinical isolates of Klebsiella pneumoniae collected from Korean non-tertiary hospitals, 24 (16.8%) showed an extended-spectrum ${\beta}$-lactamase-positive phenotype. PCR and sequence analysis revealed the presence of TEM-116 (n=13), CTX-M-3 (n=5), CTX-M-14 (n=2), CTX-M-15 (n=3), and SHV-12 (n=16). Each of the 24 isolates encoded more than one ${\beta}$-lactamase, and seven isolates (29%) harbored two different SHV-type ${\beta}$-lactamase genes ($bla_{SHV-11}$ and $bla_{SHV-12}$) bounded by insertion sequence IS26 in a single transferable plasmid.
Keywords
Klebsiella pneumoniae; SHV-11; SHV-12; extended-spectrum ${\beta}$-lactamase;
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