한우 성우 및 송아지에서 신장 및 방광의 정상적인 초음파영상에 관한 연구를 하기 위하여 본 연구를 수행하였다. 초음파 검사법에 의하여 19두의 한우 성우의 우측 신장 및 방광과 16두의 한우 송아지의 양측 신장 및 방광의 위치, 면적 및 구조를 관찰하였다. 3.5 또는 5.0-MHz convex transducer 및 5.0-MHz sector transducer를 사용하여, 신장은 요추측와 및 최후 늑간강에서 관찰하였으며, 방광은 하복부 정중선에서 관찰하였다. 송아지의 우측 신장은 6.9-9.9cm의 길이, 4.2-6.6 cm의 폭, 그리고 2.5-3.3 cm의 깊이를 나타내었다. 우측 신장의 실질 및 신동의 직경은 각각 0.77-1.26 cm 및 0.67-0.94 cm를 나타내었다. 송아지의 좌측 신장에서도 유사한 결과를 나타내었다. 성우의 우측 신장은 10.6-11.7 cm의 폭, 그리고 5.0-8.4cm의 깊이를 나타내었다. 우측 신장의 실질 및 신동의 직경은 각각 1.9-3.1 및 1.6-2.7cm를 나타내었다. 송아지에서 방광의 직경, 원주 및 면적은 각각 52.3mm, 162.8mm alc $22.3cm^{2}$이었으며, 성우에서의 방광의 직경, 원주 및 면적은 각각 94.3mm, 293.8mm alc $69.4^{2}$이었다. 본 연구에서의 초음파검사 결과는 한우 성우 및 한우 송아지의 신장 및 방광의 형태학적 변화의 진단을 위한 참고자료로서 사용될 수 있다는 결론을 얻었다.

The effect of aqua-acupuncture of total saponin on the recovery of liver function in -intoxicated rats was exemined. Rats were divided into normal, control and three experimental groups (As-I group : aqua-acupuncture of saline to Gansu locus and Aa-III group : aqua-a-cupuncture of total saponin to blank locus at the thigh). Changes of some serum biochemical parameters (ALT, AST and ALP, total protein and albumin, and albumin/globulin (A/G) ratio) were determined. Serum ALT activities were increased by $CCI_{4}$T and decreased in all aqua-acupuncture groups compared with the control group. Aa-I group showed significant decrease of ALT activities on the 2nd (P<0.05) and 3rd (P<0.05) day, respectively. Aa-II group showed significant decrease on the 3rd day (P<0.05) day. Serum AST activities were increased by $CCI_{4}$T and decreased in all apua-acupuncture groups compared with the control group. Aa-I group showed significant decrease on the 1st (P<0.05), 2nd (P<0.001) and 3rd (P<0.001) day, respectively. Aa-II group showed significant decrease on the 3rd (P<0.001) day. Aa-III group showed significant decrese on the 1st (P<0.05) and 3rd (P<0.001) day, respectrvely. Serum ALP activities were increased by $CCI_{4}$T and decreased in all aqua-a-cupuncture groups compared with the control group. Aa-I group showed significant decrease on the 3rd (P<0.01) day. Total protein, albumin and A/G ratio were decreased by $CCI_{4}$T and increased in all aqua-acupuncture groups compared with control group. Aa-I group showed significant increase only on the 3rd day in albumin (P<0.01). It was thought that the aqua-acupuncture of total saponin to Gansu locus was effective on the recovery of liver function in $CCI_{4}$T-intoxicated rats.

The aim of this study was to assess the precision of the estimates of the time of estrous cycle, optimal breeding and ovulation derived by vaginal cytology. The thirteen Korea Jin-do dogs were examined the vaginal cytology, plasma estradiol-17$$\beta $ and progesterone assay during the estrous cycle. Day 0 was the day of the first male acceptance. The main change of vaginal cytology during the estrous cycle was the high proportion of anuclear cell and erythrocyte in proestrus, superficial cell, anuclear cell and erythrocyte in estrus, parabasal cell, large intermediate cell and leukocytes in diestrus, and parabasal cell and small intermediate cell in anestrus, respectively. These data indicated that vaginal cytology was reliable method for estimating estrous cycle in Korea Jin-do dogs. In the cell indices during estrus the maximum eosinoghilic index was $92.0{\pm}$2.6 (Mean{\pm} SEM$)% at Day 2 and the maximum cornification indez was $96.0{\pm}1.3%$ at Day 2, respectively. The eosinothilic indez and cornification indez of up to 70% were found at Day -1 to Day 5 and Day -6 to Day 8, and up to 80% at Day 1 to Day 4 and Day -4 to Day 6, respectively. From these data it was presumed that eosinophilic index was more reliable index for monitoring optimal breeding time than cornification indexm because eosinophilic index peak period was shorter than cornification indeX peak period and Day 2 was the day of ovulation. Therefore, optimal breeding time was the eosinophilic index peak period, more than 80% of eosinoghilic index. The $estradiol-17{\beta}$ peak, with 3 days delayed when progesterone concentration was $4.5{\pm}0.5 ng/ml$. These data estimated that the ovulation time was the day of eosinophilic index peak, Day 2. breeding time and pvulation time in Korea Jin-do dogs.

65마리 개의 자연 발생된 종양 샘플에서 apoptosis의 수준을 알아보고자 본 실험을 실행하였다. 65 증예는 골육종 23예, 임파종 11예, 다른 형의 육종 11예, 상피종 13예, 기타 종양 7예로 구성되었다. 대분의 종양에서 다양한 범위의 apoptosis 백분율을 나타내었다. 80% 이상의 수준은 apoptosis가 전체의 55.4%(35 예)로 가장 높은 수준을 보였고, 20-39%의 수준을 보인 것은 전체의 7.7%(5 예)로 가장 낮은 수준을 보였다. 60-79%, 40-59% 및 20% 미만은 같은 수준인 12.3%(8 예)를 나타냈다. 특히 상피종에서 대부분의 증예가 69.2%) 80% 가 넘는 apoptosis를 나타내었다. 각 종류별 종양에서 평균 apoptosis의 백분율은 골육종, 임파종, 다른 형의 육종, 상피종 및 기타 종양에서 각각 72.7%, 61.4%, 71.1%, 74.8% 및 79.7% 이었다.

The study was conducted to determine normal blood and serum chemical values on deer. Normal blood and serum chemical values were studied in 8 healthy deers bred at Dalseung Park in Taegu. The values were analyzed with a blood chemical analyzer and calculated by Chawen's statistical analysis. The blood values of RBC, WBC and PCV were $9.73{\pm}1.19{\times} 10^{6}/{\mu}l, 3.37{\pm}0.88{\times}10^{3}/{\mu}l, 47.25{\pm}4.65 % respectively. The serum chemical value were: GOT; 83.0{\pm}39.4 IU/L, GPT; 50.12{\pm}25.83 IU/L, BUN; 36.63{\pm}8.70 mg/dl. CPK ; 1,296{\pm}495 IU/L, ALP; 238{\pm}54 IU/L, glucose; 143{\pm}52 mg/dl, total protein; 6.95{\pm}0.61 g/dl, amylase ; 116.0{\pm}34.5 IU/L,$ respectively. These values will be clinically helpful to diagnose the disease of deer.

Malassezia pachydercatis균은 개 외이염의 주된 원인균으로 알려져 있으나, 그 감염률은 지역에 따라 많은 차이가 있다. 그러나 그 기병론적인 역할에 대해서는 아직 명확히 밝혀지지 않았다. 이에 본문에서는 국내에서 사육되는 개의 외이염에서 Malassezia pachydercatis 균의 분리율을 조사하고, Malassezia pachydercatis에 대한 혈중 항체와 외이염과의 연관성을 밝혀 Malassezia pachydercatis에 의한 외이염 진단을 위한 도구로서 간접 ELISA 법의 유용성을 평가해 보고자 본 연구를 수행하였다. 총 112두 중, 외이염에 감염된 개 44두와 정상견 68두를 대상으로, 심부외이도에서 취한 가검물을 배양검사하였고, Malassezia pachydercatis 항원에 대한 혈청내 IgG와 IgM 역가를 간접 ELISA법에 의해 측정하였다. 살아있는 Malassezia pachydercatis 균을 3두의 정상견 외이도에 접종하여 Malassezia pachydercatis 균의 실험적 감염에 대한 조직병리학적 소견, 조직내 침투 정도, 그리고 접종 후 시간경과에 따른 IgG와 IgM의 혈중 역가변화를 측정하였다. Malassezia pachydercatis 균은 외이염이 있는 개 귀에서 가장 높게 분리(50.0%)되었고, 정상귀에서는 낮게 분리(6.3%)되었다. Malassezia pachydercatis 항원을 이용한 간접 ELISA 법 진단에서, Malassezia pachydercatis 양성배양인 외이염 개체에서 IgG와 IgM에 대한 민감도는 각각 41.7%, 20.8%이었고 특이도는 88.2%, 85.2% 이었다. 실험적으로 Malassezia pachydercatis 를 접종한 3두중 1두에서만 조직병리학적 변화와 모낭에 Malassezia pachydercatis 균체가 확인되었으며, IgG와 IgM 혈중역가도 유의성 있게 증가하였다. 국내에서 사육되는 개 외이염의 주된 원인균은 Malassezia pachydercatis 균이었고 이 균의 혈중항체는 유의성 있게 증가하였으며(p<0.005), 간접 ELISA법은 Malassezia pachydercatis 에 의한 개외이염의 유용한 진단도구임을 증명하였다.

In this paper the development of an automatic body temperature measuring system which can be attached to the milking machine has been studied. Since the disease is highly related to the body temperature of the cattle, early detection of the abnormal temperature would prevent the severe problems which may occur in dairy farms. The electronic component AD590 is used as a temperature sensor for the system. The device is highly robust against the noise since the output signal is the current. So it can be applied to the long distance sensing. The resolution of the signal is $0.1{\circ}C$ and the current is 10 mV. Also the A/D converter is designed for interfacing the sensor with a computer. A temperature measuring experiment using the developed system has been done for measuring the temperature of human beings and the system was proven to be useful for measuring the body temperature of the dairy cattle properly.

The effectiveness of sodium carboxymethylcellulose (SCMC) and deztran in the prevention of adhesion formation on the uterus and embryo collection in rabbits was elucidated. Following induction of adhesion on uterus and uterine horns by abrasion and retrograde flushing of embryos in gonadotropins primed rabbits, the solutions of saline (for control), 1% SCMC, 10% dextran and a synthetic solution of 1% SCNC and 10% dextran in saline were infused in the abdominal cavity at the dose of 5 ml/kg of body weight. The average percentage of adhesion was 11.1, 28.6, 41.7 and 73.3% in the rabbits infused with the synthetic, 1% SCMC, 10% dextran and saline solutions, respectively. The synthetic solution was more effective than other solutions in the rabbits. The average number and recovery rate of embryos were significantly (P<0.01) higher in the synthetic solution group than 1% SCMC, 10% dextran or saline solution groups. Among the collected embryos in the groups, the distribution of the normal embryos was higher in the synthetic solution group (99%) and the 10% dextran solution group (95.7%) than the 1% SCMC solution group (78.4%) and saline (66.2%). Theretore, a synthetic solution which is combined with 1% SCMC and 10% deztran in saline can be effectively used for the prevention of adhesion formation after uterine surgery and embryo collection.

This study was carried out to evaluate the efficiency of production of cloned embryos by nuclear translatation (NT) when using 4-cell to compact morula stage embryos as nuclear donor. In micromanitulation and electrofusion of blastomeres from 4-cell to morula stage embryos, the successful injection rate was higher with late stage blastomeres, on the contrary the fusion rate was lower. The in vitro developmental rate of NT embryos was not significantly different between cell-stages of donor blastomeres. Although the overall rate of production of cloned embryos with 4-cell. 8-cell, early and late morula stage embryos was 14.0, 18.0, 15.3 and 14.1%, respectively, the mean number of blastocysts produced with a donor embryo was the most (4.51) with the compact morulae. Therefore, it can be suggested that the embryos at thelate stage is more beneficial for the mulciple production of cloned embryos, If the late stage blastomeres have maintained their totipotency to produce intact offspring.

Ketosis can exist in both clinicla and subclinical forms. Detection of the subclinical form of ketosis by the use of a nitroprusside based test powder gas been shown to be a relatively simple and reliable procedure. Howere, very liffle is known about the indcidence or prevalence of the subclinical ketosis in Korea. In the present study, samples of urine taken from 288 dairy cows in Chonnam area were examined to demonstrate the presence of ketone bodies, using ross test. All the cows were within 4 weeks either before or after parturition. The gerd size was from 10 to more than 100 cows, and the cows did not show any clinical signs of ketosis. Of the 288 cows, 85 (29.5%) were positive to Ross test; of the positive cases 42.4% were +, 44.7% were ++, and the remaining 12.9% were +++. The prevalence increased from 3rd parity, peaked at 6th parity and thereafter decreased. The prevalence was higher after parturition than before parturition, with the highest occurrence during the 2nd week after calving. The prevalence was higher during winter. Also cows kept indoor throughout the year showed higher occurrence of the subclinical ketosis. The subclinical ketosis did not affect the specific gravity of the urine; however, increasing amount of ketone bodies in the urine decreased the pH of urine.

Comet assay 는 포유류 세포에서 DNA의 파괴를 측정하는데 있어 신속하고 단순하며 시각적이고 민감한 방법이다. Apoptosis에서는 세포핵의 광범위한 DNA의 붕괴가 일어나므로 comet assay는 종양세포에서 apoptosis가 발생되었는가를 알아내는데 유용하다. 본 연구는 apoptosis 연구의 결과가 변화되지 않도록 comet assay slides를 좀 더 오래 보관할 수 있는 방법을 개발하고자 시행되었다. 개의 종양세포를 가지고 alkali comet assay를 끝낸 뒤 slides를 진공 건조기에서 꺼내서 증류수로 점적하여 10-20분간 침수시키고 현광현미경하에서 육안적으로 관찰하였다. 건조후 3-4일, 1주, 2주, 3주, 4주 및 7주의 slides에서 apoptosis 회복율(%)은 각각 98.1, 98.3, 99.4, 80.8 및 35.2%이었다. 3주 이내의 slides에서 대조군과 비교하여 apoptosis 회복율에서 차이가 없었으나 4주 이상의 slides를 건조후 침수시키는 방법을 이용하였을 때 apoptosis 평가에서 건조 후 3주간까지는 처음의 결과와 차이가 없으며, 이 방법을 이용하여 comet slide의 좀 더 긴 기간이 보관과 보관후의 재평가에서 이용될 수 있는 좋은 방법이 된다.

This study was performed to analyse the common calls of poddle spectrographically : bark, growl, howl, snore, yelp and whine. The sonograms of 6 common calls were shown their own specific features. There were significant differences among each types of common callsin the parceter of minimun frequency of call (MIFC), maximun frequency of call (MAFC), duration of call (DC), interval between call (IBC), dominant frequency (DF), F1 formant, F2 formant and F3 formant (P<0.01). It was considered that it was possible to record the main common calls dogs by sonograms and it sould be applied to objective basic data for understanding the psychological stats of dogs, the social relationship among them and the relationship sith human being.

In this study, dldctronic identification system of individual dairy cow was developed for autocatization of stoxkvreeding management. To automize the breeding management, it is necessary to obtain and analyze the individual information distinguished from others perferentially. Electronic identification system can distinguish individual livestock from others with electromagnetic wave signal recognition system. Electoronic identification system consists of transmitter transmitting the oscillated signal and receiver set. The transmitted signal from transmitter clung to individual livestock is received from the receiving antenna and the signal in different according to the established value of the register. By distinct signal recieved from the reciever, wi can distinguish the identity of a livestock from others clearly. This system can manage $2^{12}$ individuals with a reciever theoretically. However in order to reduce the errors by analogous signal, this system uses only triple number and can manage 1365 individuals with a reciever practically. This system can be connevtted to Max 232 and microcomputer for the breeding management efficiently.

This study was performed to investigate the effects of xylazine on the motility of abomasum and proximal colon in cattle. Bipolar electrodes were implanted in the subserosa of the abomasum and colon. Electromyogram of the motility was recorded by polygraph after intramuscular administration of xylazine at the doses of 0.1 and 0.2 mg/kg. The mtility of abomasum was completely inhibited at 5~11 minutes after xylazine administration. However, that of the abomasum was reappeared at 15 minutes and greatly increased to supranormal motility at 41~51 minutes, then recovered to normal activity at 70~123 minutes. Meanwhile, the motility of proximal colon was completely inhibited at 3~10 minutes and reappeared at 60~150 minutes after xylazine administration, then recovered gradually to normal pattern at 240 minutes. Our data indicate that xylazine (0.1~0.2 mg/kg) affect on the abomasal motility in a biphasic manner, initial inhibition and following activation, but on the colon motility in a monophasic inhibition.

A comparative study of three absorbable suture materials of chromic catgut, Dexon II (a polyglycolic acid suture with a polycaprolate coating system) and Coated Vicryl (a polyglactin 910 suture with a glycolide-lactide-calcium stearate coating system) was undertaken in terms of tensile strength, breaking elongation, appearances of the suture materials in the subcutaneous layer of rats and of tissue reaction in the intramuscular layer of rats. The initial tensile strength of chromic catgut and the tensile strength after 21 days were about 1.55 kg and 0.19 kg, respectively. Those of Dexon II were about 2.01 kg and 0. 20 kg, respectively. Those of Coated Vicryl were about 2.39 kg and 0.48 kg, respectively. Coated Vicryl showed the highest tensile strength among the three materials during the whole period. On the other hand, Dexon II showed the highest breaking elongation among the three materials during the first week. But the breaking elongation of Dexon II rapidly continuously decreased during the whole period. The appearances of the suture materials in the subcutaneous layer of rats showed that chromic catgut was the fastest among the three materials, whereas it formed comparatively much connective tissue. The intramuscular absorption rate and tissue reaction of Dexon II were similar to those of Coated Vicryl. The intramuscular absorption rate of these was fairly late when compared with chromic catgut. And the tissue reaction appearance of these showed the formation of a granuloma with foreign body giant cells, macrothages and epitheloid cells by the 28th day in the experimental period.

제주도에 서식중인 흑기러기(Branta bernicla)가 식욕감퇴, 점진적인 수척, 호흡촉박 등을 나타내다 폐사하였다. 부검소견으로서는 폐 및 복부기낭에 다수의 위약한 흰색-황색 결절이, 병리조직학적검사결과 괴사병소에 균사와 Aspergillus의소포가 확인되었다. 배양결과 Aspergillus fumigatus 감염증으로 진단된 국내 최초의 보고로 생각된다.

일반적인 치료에 반응을 나타내지 않는 호흡곤란증에 걸린 4년령의 Shepherd개에서 피부, 눈, 림프절의 병변을 동반한 치명적인 파종성 크립토콕스병이 진단되었다. 세포학적검사에 의해 난원형 내지 구형이고, 두꺼운 협막을 갖고 있는, 형태학적으로 Cryptococcus neoformans와 일치하는 효모균이 증명되었다. 이 병원체는 혈액, 오줌, 콧물, 견갑전림프절 흡인물, 피부생검 시료, 피부 면봉 시료 등을 25$\circ $C의 Pal씨 배지에 접종하여 용이하게 분리되었다 배양물을 PHOL염색액으로 염색하여 현미경으로 검사한 결과 얇은 막에 싸인 발아를 나타내거나. 나타내지 않는 구형 내지 난원형의 효모균이 증명되었다. 이 개는 ketaconazole 로 치료를 시작 한 후 6일만에 폐사하였다. 공기, 흙, 비둘기 배설물, 톱밥 등을 Pal씨 배지에 접종하여 C.neoformans가 배양됨으로써 역학적으로 환경이 병원소 역할을 한 것으로 판단되었다. 환축과 환경으로부터 분리된 균주는 세밀한 동정결과 neoformans (serotype AD)에 속하며, Filobasidiella neoformans "alpha" mating type을 나타내었다. 이 연구결과, Pal씨 배지가 크립토콕스병의 조기진단과 역학적 조사에 훌륭한 감별배지라는 것이 입증되었다. 개량된 Pal씨 배지는 C. neoformans의 genetic crossing을 판단하는데 성공적으로 사용할 수 있었다.

Fiberglass may be a pulmonary toxin and carcinogen because it imitates asbestos, as do other man-made mineral fibers. A 5-months-old Jind dog was accidentally exposed to fiberglass extensively for 3 months in a site of apartment construction. Canine acute clinical pneumoconiosis caused by fiberglass was diagnosed by history, ezamination of environment, physical examination, and chest x-ray check of patient. The main signs were anorexia, dyspnea, increased irregular opacities and air trapping in lung field of chest x-ray films. Because the fiverglass which is used for several purpose are dangerous for health of animal and human. fiberglass dust must be controlled to minimize the adverse effects of fiberglass in the environment.

The left tusk of Asian elephant (Elephas maximus) was splitted longitudinally and its middle portion avulsed by slip. The pulp was exposed and bled. A loosened tusk fragment continued to irritate its sulcus. We performed a partial pulpotomy and pulp capping. We filled the wide space between the loosened tusk fragment and the counterpart with zinc oxide eugenol and zine phophate cement but it was failed, and then we fastened the loosened tusk fragment to the counterpart by gauze and plaster. The ollsened tusk fragment grew to drop 100 days after the insult and main portion with pulp capping was healthy.

Chemotherapy of transmissible venereal tumor (TVT) in a dog has been tried, using vincritstine, cyclophosphamide, and methotrexate. The dog was hiven an combination chemotherapy and underwent complete regression of the tumors with on recurrence. The result of our clinical trial indicates that combination chemotherapy is an effective modality for dogs with TVT.