Objectives : In order to evaluate the cytotoxicity of hexavalent chromium, the cytoprotective effect of phenolic compounds against hexavalent chromium-induced cytotoxicity, cell viability, cell adhesion ability, lactate dehydrogenase(LDH) activity, and morphological changes of cells were examined. Methods : We measured the cytotoxicity of hexavalent chromium with 3-[4,5-dimethyl-thiazol-2-yl]-2,5-diphenyltetrazolium bromide(MTT), 2,3-bis-[2-methoxy-4-nitro-5-sulfophenyl]-2H-tetrazolium-5-caboxanilide (XTT), LDH and DPPH methods. Results : The cytotoxicity of hexavalent chromium($IC_{50}$, $44.0-51.0{\mu}M$) was high according to the toxic criteria. Cytoprotective effect of phenolic compounds against $IC_{50}$ value of hexavalent chromium in cell morphology increased in a concentration-dependent manner. Conclusions : These results suggest that 3,4,5-trihydroxybenzoic acid may be used as a cytoprotective agent against chromium(IV)-mediated cytotoxicity.

Objectives : This study was investigated to the relationship ayurvedic constitution and each skin hydration and pH. Methods : The questionaire survey and skin analysis of female students attending university located in Chugnam was carried out in order to assess skin types according to ayurvedic constituion such as vata, pitta and kapha. Data analysis were used describe statistics, ANOVA in Duncan's multiple comparative test and Pearson's correlation by SPSS. Skin hydration and pH was measured by Corneometer and skin-pH meter. Results : The results of this study are as follows. First, in terms of knowledge of ayurvedic constitutional types most students are not aware of that. Second, the most common constitution in the ayurveda medical system was pitta. Third, in the analysis of skin type by constitution of ayurveda, vata had dry skin while pitta and kapha tended to have more combination skin. However, this result didn't show any statistically significant difference either. Forth, the analysis of skin tone by constitution of ayurveda showed that vata was white, and pitta and kapha were just average. Discussion and Conclusion : The results of this study was shown that the difference between Korean and Indian, so we should develop effective diagnosis tool for Korean people. In the future, we expect that ayurvedic skin and body program developed by this study spread and practical use at esthetic industry and domain of esthetics in Korea.

Objective : This study was to examine a relationship among subjective psychological stress, physical stress response and oxidative stress. Methods : The subjects were consisted of 87 healthy volunteers. To assess subjective psychological stress, SRI(stress response inventory) was completed. To assess psychological and physical stress response, HRV(Heart Rate Variability) were tested. To assess oxidative stress, d-roms(Derivatives of reactive oxygen metabolites test) test was conducted. Subjects were divided into 3 groups depending on SRI(Stress Response Inventory) score, low stress response(LSR: lower than 30 percentile), Medium(MSR: 30-70 percentile) and high stress response(HSR: higher than 70 percentile). The Relationship between Stress Response and Oxidative Stress was estimated by correlation and One-way ANOVA analysis. Results : There were no significant differences of demographic data among 3 groups. There was a significant difference of oxidative stress among 3 groups. Conclusion : Our results suggest that there is a weak positive correlation between subjective psychological stress and oxidative stress. There is a significant difference of oxidative stress between HSR group and LSR group.

Objectives : In order to evaluate the cytotoxicity of 3,4,5-trihydroxybenzoic acid and related compounds on the growth of normal cell lines and human oral epithelioid cell line, cell viability, cell adhesion ability, and morphological changes of cells were examined. Methods : We measured the cytotoxicity of 3,4,5-trihydroxybenzoic acid and related compounds with 3-[4,5-dimethyl-thiazol-2-yl]-2,5-diphenyltetrazolium bromide-[MTT), and 2,3-bis-[2-methoxy-4-nitro-5-sulfophenyl]-2H-tetrazolium -5-caboxanilide-[XTT) methods. Results : The cytotoxicity of 3,4,5-trihydroxybenzoic acid($IC_{50}$, $2,552.40\;{\mu}M$) was low according to the toxic criteria. Cytotoxic effect of 3,4,5-trihydroxybenzoic acid and related compounds against $IC_{50}$ value in cell morphology increased in a concentration-dependent manner. In light microscopy, $100\;{\mu}M$ 3,4,5-trihydroxy-benzoic acid showed th highest cytotoxic activity. Conclusions : These results suggest that 3,4,5-trihydroxybenzoic acid may have a potential anticancer activity.

Objective : This research was aimed to investigate the anti-tumor effect, safety, mechanism and metabolizing enzyme of Agrimonia pilosa LEDEB(APL) in female C57B/L mouse. Methods : At first, to evaluate the anti-tumor activity of APL, we divided into four groups, normal, control, APL100(100mg/kg), APL150(150mg/kg). LLC obtained American Type Culture Collection was used. LLC had been inoculated to induce tumor. To measure the anti-tumor effect of APL, we calibrate tumor size and weight. To study for mechanism of anti-tumor in APL, we used western blotting and to know metabolizing enzyme in APL we used to real-time PCR. Results : APL100, APL150 inhibited tumor growth after medicine injected. APL did not only induced caspase-dependent apoptosis in LLC-bearing mouse tumor. In APL100, it were decreased 72% in CYP3A11. In APL150, it were decreased 62%, 75% in CYP3A11 and MRP1a respectively. Conclusion : These results suggests that APL has some anti-tumor effects in female C57B/L mouse tumor. APL should be careful use with other drugs related with CYP3A11 or MRP1a.

This study was to examine of physical and psychological traits to sasang constitution in college students. The subjects in this study were 114 people, who were consists of 35 persons of Taeeumin, 34 persons of Soyangin and 45 persons of Soeumin. We were analyzed psychological trait with the Myers-Briggs Type Indicators(MBTI) and State-Trait Anxiety Inventory(STAI) according to sasang constitution. Also, We were analyzed physical trait with the Body Mass Index(BMI) according to sasang constitution. Each sasang constitutions personality type showed significantly differences only in the extraversion and introversion(p=0.012). In the relationship of BMI, Taeeumin showed bigger BMI compared to others (p<0.001). As a result of logistic regression analysis, Soyangin was higher in the extraversion(odds ratio[OR], 3.48 ; 95% confidence interval [95% CI], 1.18-10.26) compared with Taeeumin. Also, each sasang constitutions showed significantly differences in trait-anxiety(p=0.047). The score of trait-anxiety in Soeumin was significantly higher than that in Taeeumin. The limitation of this study was that particular samples could not represent the population, because the students from one college had participated in this study. In the future, we will perform this study in general population. At the same time, we will also develop approaches considering physical and psychological aspects depending on the types of constitution in the management of subjects.

Purpose : To evaluate the effects of Samultang(SMT) on the metals in the dams and fetuses Method : SMT(Decoction) was administered orally using different concentrations to pregnant Sprague-Dawley rats for 20days after being assigned randomly to 5 groups. Each group has 11 dams. Pregnant rats were sacrificed at the 20th day of gestation and metal concentration in the dams and fetuses was observed using a ICP-MS and mercury analyzer. Results : Hg concentration in the blood of dams was lower in the $SMT{\times}1$, $SMT{\times}2$, $SMT{\times}4$group than VC(Vehicle Control) and Control group. Hg concentration in the organs of dams was lower in the $SMT{\times}1$, $SMT{\times}2$, $SMT{\times}4$ group than VC, Control group. Whereas Cd concentration in the organs of fetuses was getting lower as increasing SMT dose, Hg concentration in the organs of fetuses was getting higher as increasing SMT dose. Discussion and Conclusion : There were not any significant harmful differences of metal concentration in the dams and fetuses treated by SMT. Particularly, SMT showed the possibility of efficacy to reduce the concentration of Hg in dams but to increase the concentration of Hg in fetuses. The mechanism of reduction is not yet proven. A hypothesis says there is a possibility of the chelation effect between the essential elements of herbs and heavy metals. Another hypothesis is the possibility of Hg transmission from dam to fetus. Further study should be conducted to make guidelines for the safe use of herbal medicine.

Objectives : The purpose of this study was evaluated hypoglycemic effect of culture broth of germanium-fortified Tricholoma matsutake mycelium and yeast. Methods : We examined $\alpha$-glucosidase inhibitory activity, blood glucose level, concentration of serum lipid, and serum metabolic variables of Tricholoma matsutake mycelium and yeast fortified Germanium. in streptozotocin induced diabetic rat. Results : In the $\alpha$-glucosidase inhibitory activity, germanium-fortified yeast was significantly higher than germanium-fortified Tricholoma matsutake mycelium. The hypoglycemic effects of germaniumfortified Tricholoma matsutake mycelium was higher than germanium-fortified yeast. The activity of alkaline phosphatase(ALP), aspartate aminotransferase(AST) and alanine aminotransferase(ALT) was significantly lower in the germanium-fortified Tricholoma matsutake mycelium and yeast than in diabetic control(DC) group and diabetic positive control(PC) group. The concentration of total cholesterol and triglyceride of germanium-fortified yeast was significantly lower than germanium-fortified Tricholoma matsutake mycelium, DC group and PC group. Conclusions : The results suggest that germanium-fortified Tricholoma matsutake mycelium and yeast have improvement effects in blood glucose, serum lipid and liver function.

This study was performed to evaluate the effect of HCB on the bone mass and its related factors in estrogen-deficient animal model. The model rats of osteoporosis showed a significant decrease in bone density, bone ash density, calcium content of femur bone. At the 14th day after ovariectomy-surgery, rats were administered with HCB, extract of Houyttnia cordata, per orally, and continued for 10 weeks. And osteoporosis related parameters were determined to investigate the effect of HCB. Osteoporotic rats showed lower serum estrogen level, higher body weight than normal rats, and showed atrophy of uterine horns.

Background : Currently, ontology research has led the trend of technical development in medical informatics area. For Korean medicine, the reference terminology should be developed to facilitate the ontology research. Objective : This research aimed to design the management system for traditional Korean medical terms. Method :We built the internet-based system in which multi-users work simultaneously by using the relational database system(SQL Server2005) and visual studio 2005. Result : By this system, researchers can collect, refine, and inspect Korean medicine terms efficiently, and the terms can be transcribed into synonym, Korean, Chinese, and simplified Chinese. It enables the terms be input into the system accurately and managed by its classification. Conclusion : We developed the concept groups and its hierarchy system for Korean medicine terms which provides the basis for ontology system.

Background and Objectives: The purpose of the study was to explore the attitude of workers toward a system of collaborative hospital practice between western and traditional Korean medicine, to identify factors influencing this attitude, and discuss the reasons socioeconomic groups' differences. Method: The data were collected with a questionnaire for this study from 14 April 2009 to 1 May 2009. Data were analyzed mainly via non-parametric statistics and logistic regressions utilising SPSS 17.0 (Statistical Package for the Social Sciences) to determine the workers' attitude about the hospital system and to predict factors contributing to positive attitudes. Results: A total of 1,260 workers working for large factories in Gwangju Metropolitan City. Findings confirmed that more than 40% of the workers show interest in the system and about 44% of the workers also express positive attitudes. Factors found to influence the workers' response included marital status, income level, health status, experience in complementary medicine services, the number of health care facilities' visit. Conclusions: The prospects to establish the system of collaborative hospital practice as reflected by the workers' view about the Korean health care service delivery system. Their attitudes toward the system differed among socioeconomic groups. Government and health care providers should identify the socioeconomic subgroups' demands and opinions in order to find and develop measures of integrating western and traditional Korean medicine in health care facilities.