Ultrastructural changes of Entamoeba histolytica, a concomitant strain YS-9, which was treated in the immune serum was examined. The amoebae in the serum became immobilized state from about 30 minutes of the treatment and recovered at about 60-90 minutes. In the cells of control group, helix structures were scattered throughout the cytoplasm. The particles comprising the helix structure averaged 20 nm in diameter. At the beginning stage of the immobilization, helical aggregates(chromatoid body) which associated with vacuoles appeared abundantly in the cytoplasm, but gradually tended to aggregate along peripheral region of the cell, specially in intactly immobilized state. Each parallel array of aggregates measured about 45 nm in width. When the cells became remobilize, pseudopodia appeared again, but helical aggregates disappeared and numerous helix structures were observed in the cell periphery. Distribution of glycogen particles showed no change, and acid phosphatase activities were seen in both the immobilized and the control group. The reaction was markedly noticed in the vacuoles.

방아깨비의 종형유충을 재료로 하여 혈구의 미세구조를 관찰한 바 4 가지의 혈구 형태를 구분해 냈다. Prohemocyte는 크기가 가장 작으며 미분화된 소기관을 가졌고 Plasmatocyte는 그 수가 비교적 많고 발달된 소기관을 가졌으며, Granular cell 은 방추형 또는 난형으로 세포질내에 몇가지 과립을 가진 것이 특징이다. Adipohemocyte는 많은 지방과립을 함유하고 microtubule 특징적으로 나타났다.

This experiment was undertaken in order to localize the labeled dbcAMP (dibutyryl cyclic AMP) in oocytes whose development has been suppressed by cold dbcAMP for 6 or 19 hours in vitro. Mouse oocytes were obtained from the ovaries of 3-4 week old A strain female mice, by puncturing the Graafian follicles in the modified Krebs-Ringer bicarbonate salt solution under the dissecting microscope. Those oocytes which have intact germinal vesicle were cultured in the basic culture medium supplemented with 0.4% bovine serum albumin (BSA). Cultivation of the oocytes was carried out in a microtube developed by Cho (1974). The cultures were then incubated in a humidified 5% $CO_2$ incubator maintained at $37^{\circ}C$ for 6 or 19 hours (Donahue, 1968). DbcAMP was added to culture medium for a final concentration of 100ug/ml, and $^3H-dbc$ AMP (specific activity 13 Ci/mM) for a final concentration of $40{\mu}Ci/ml$ was also added to the medium. For electron microscopic autoradiography, those oocytes recovered from the culture were washed with phosphate buffer (pH 7.4), and immediately prefixed in a 2.5% glutaraldehyde overnight and postfixed for 2 hours at $4 ^{\circ}C$ in 1% osmium tetroxide in phosphate buffer with pH 7.4 (Palade, 1952). After fixation, the materials were dehydrated in graded alcohol series and embedded in Epon 812 mixture based on the standard procedures (Luft, 1961). The thin sections $600-700{\AA}$ thick were mounted on the grids of 200 meshes. The grids containing sections were coated with a nuclear emulsion Kodak NTB-3 and stored in a cold dark box (at $4^{\circ}C$) for 3 weeks. After exposure, the samples were developed with Kodak D-19 and stained with uranyl acetate and lead citrate. Routine observation was made with Hitachi HU-11E electron microsocope. The results of the observation were as followings: 1. It was found that the labeled dbcAMP penetrated the egg plasma membrane and dispersed at random in the cytoplasm. 2. It was also observed that most of the labeled dbcAMP was attached to microfibrillar lattices portion of the oocyte cytoplasm. There fore, it is presumed that the receptor of the dbcAMP is localized in the microfibrillar lattices of the oocyte. 3. It also seems that some other cell organells such as mitochondria, Golgi complex, cortical granules are not directly related to the action of the dbcAMP. 4. The labeled dbcAMP was neither observed in the membrane nor in the nucleus. Therefore, it seems that there is no relationship between the concentration of dbcAMP and the nuclear membranous permeability. 5. There was no difference in number of dbcAMP particles when oocytes were cultured for 6 hours and 19 hours. 6. However, it was observed that, in same of the oocytes suppressed in germinal vesicle by dbcAMP for 19 hours, cell organells were moved and concentrated to a small portion of the cytoplasm, and that the morphology of the organells greatly changed to an abnormal. form. Therefore, it is supposed that those oocytes were in the process of degeneration. From the above results, it is expected that dbcAMP penetrated the egg membrane and was bound to the receptor which seems to be located in the microfibrillar lattiees portion, and that this dbcAMP-receptor complex inhibited some enzyme system of the oocytes which are essential for the germinal vesicle breakdown.

용탕의 온도는 탄소의 비율을 높이기 위하여 $1500^{\circ}C$ 이하로 해야 하며, 주입온도는 $1460^{\circ}C$ 내외가 적당하다. 주조상태에서 소지의 침상석출물은 orthorhombic $(Fe,\;Mn)_3C$로 판명되었다. 냉간압연비는 클수록 결정이 미세화되고 또한 경도도 증가하였다. 표면탈탄이 얼어 나면 표면층에 martensite가 형성되어 성형하기가 어려우므로 argon 분위기를 쓰는 것이 좋았다. 두께 1 mm 판재의 경우 수인처리 온도가 $950^{\circ}C$ 이하일 때 탄화물 stringer 가 나타났고, $1020^{\circ}C$ 에서 30 분간 가열했을 때 탄화물이 의 소지에 고용되었다. C 함량이 클수록 인장강도와 연신률이 증가하였다. Ni, Cr 및 V 을 미량 첨가했을 때 인장강도 및 연신률이 커졌으며 또한 굽힘성도 좋았다.