This study was performed to compare the kinematics among three different safety shoes(type 1: ergonomically designed and high quality shoes, 2: curved and cushioned safety shoes, and 3: regular safety shoes) and to find the effect of insole during walking. Ten healthy subjects were recruited for this study. The range of motion of knee and ankle joint, angle of rear foot and angle of heel contact were measured using a three dimensional motion analysis system. In the second peak, the angle of heel contact showed statistically significant difference between safety shoes and insole, however, there was no statistical significance among three different safety shoes. The angle of ankle increased significantly at initial contact, first peak, the second peak and the toe off phase compared with type 1 and 2 safety shoes, and the angle of ankle showed statistically significant difference between with and without applying the insole. During the first peak, the second peak and the toe off phase, the angle of knee was statistical significance between safety shoes and insole. In heel contact, the angles of Achilles' tendon showed statistically significant difference between safety shoes and insole. The rear foot angles showed statistically significant difference between safety shoes and insole during heel contact and early heel contact. These results suggest that the type 1 safety shoes were superior to others in the statistics, and applying insole could be a possible method to prevent fatigue of lower extremity and musculoskeletal disorders. Further studies are needed to find the effect of ergonomically designed safety shoes and insole on practical value in prevention of musculoskeletal disorder, fatigue and satisfaction of workers.